CuGenDBv2

Gene ID	MC05g1222
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	mediator of RNA polymerase II transcription subunit 27
Genome location	MC05:16500034..16503032
RNA-Seq Expression	MC05g1222
Synteny	MC05g1222
Gene Ontology terms	GO:0006357 - regulation of transcription by RNA polymerase II (biological process) GO:0045893 - positive regulation of transcription, DNA-templated (biological process) GO:0016592 - mediator complex (cellular component) GO:0003713 - transcription coactivator activity (molecular function)
InterPro domains	IPR021627 - Mediator complex, subunit Med27

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

XP_022151438.1 mediator of RNA polymerase II transcription subunit 27 [Momordica charantia]

4.43e-287

95.28

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Query:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ
        MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN

Query:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA
        EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA
Subjt:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA

Query:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP
        FFAPDE                    EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP
Subjt:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP

Query:  ISSLKDQRSDPVQAFHIGCFSEEL
        ISSLKDQRSDPVQAFHIGCFSEEL
Subjt:  ISSLKDQRSDPVQAFHIGCFSEEL

XP_022940393.1 mediator of RNA polymerase II transcription subunit 27 [Cucurbita moschata]

1.57e-250

84.12

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Query:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ
        MRQTQ PAQG TATVSP T   HS GSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTA PHQSTKPLHLFQKED SMRQHL DLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLD+E+  N+EPA KKPCFSHGVT++KQ+ELN
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN

Query:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA
        +  +LSDILTCMEKEVPDLKIFTYERLDWLKQA AL SQAN+NSIGALKEHNYHSPRM P SL A+S DK +VIELIIPSVFRAVVSLHPVGSVEPDAVA
Subjt:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA

Query:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP
        FF+PDE                    EHAATVLQYF+GIQ KAAL+PLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTS++ S VP
Subjt:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP

Query:  ISSLKDQRSDPVQAFHIGCFSE
        ++SLKDQ+ D VQAFHIGCF+E
Subjt:  ISSLKDQRSDPVQAFHIGCFSE

XP_023005694.1 mediator of RNA polymerase II transcription subunit 27 [Cucurbita maxima]

6.37e-250

83.89

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Query:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ
        MRQTQ PAQG TATVSP T   HS GSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTA PHQSTKPLHLFQKED SMRQHL DLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLD+E+  N+EPA KKPCFSHGVT++KQ+ELN
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN

Query:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA
        +  +LSDILTCMEKEVPDLKIFTYERLDWLKQA  L SQAN+NSIG LKEHNYHSPRM P SL A+S DK +VIELIIPSVFRAVVSLHPVGSVEPDAVA
Subjt:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA

Query:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP
        FF+PDE                    EHAATVLQYF+GIQ KAAL+PLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTS++ SAVP
Subjt:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP

Query:  ISSLKDQRSDPVQAFHIGCFSE
        ++SLKDQ+ D VQAFHIGCF+E
Subjt:  ISSLKDQRSDPVQAFHIGCFSE

XP_023539656.1 mediator of RNA polymerase II transcription subunit 27 [Cucurbita pepo subsp. pepo]

4.70e-252

84.6

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Query:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ
        MRQTQ PAQG TATVSP T   HS GSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTA PHQSTKPLHLFQKED SMRQHL DLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDE+  N+EPA KKPCFSHGVT++KQ+ELN
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN

Query:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA
        +  +LSDILTC+EKEVPDLKIFTYERLDWLKQA ALSSQAN+NSIGALKEHNYHSPRM P SL A+S DK +VIELIIPSVFRAVVSLHPVGSVEPDAVA
Subjt:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA

Query:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP
        FF+PDE                    EHAATVLQYF+GIQ KAAL+PLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTS++ SAVP
Subjt:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP

Query:  ISSLKDQRSDPVQAFHIGCFSE
        ++SLKDQ+ D VQAFHIGCF+E
Subjt:  ISSLKDQRSDPVQAFHIGCFSE

XP_038904949.1 mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida]

1.33e-249

83.49

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Query:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ
        MRQTQQPAQGQTATVSPNTA PHS  SA++APPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTA PHQSTKPLHLFQKED SMRQHL DLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL+DE+  N EPALKKPCFSHGVT++KQ+ELN
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN

Query:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA
        + R+LS+ILTCMEKEVP+LKIFTYERLDWLKQA AL SQAN+NSIG LKEH+YHSPRM PGSL A+  DK  VIEL+IPSVFRAVVSLHPVGSVEPDAVA
Subjt:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA

Query:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP
        FF+PDE                    EHAATVLQYFLG Q KAALYPLLLWICSYQNLFSKACSKCG+LLSVNKQSDLLLPPA+RPYKQFC S+  S+VP
Subjt:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP

Query:  ISSLKDQRSDPVQAFHIGCFSEEL
        I+SLKDQ+ D VQAFHI CFS EL
Subjt:  ISSLKDQRSDPVQAFHIGCFSEEL

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LGT1 Uncharacterized protein

2.77e-241

81.37

Show/hide

Query:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ
        MR  QQP+QGQTATVSP+T   HS  S ++APPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTA PHQS+KPLHLFQKED SMRQHL DLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL+DE+  N EPALKKPCFSHGVT++KQ+EL 
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN

Query:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA
        + R+LSDIL+CMEKEVP+LKIFTYERLDWLKQA AL SQAN+NSIGALKEH+YHSPRM PG+L A+  DK  VIELIIPSVFRAVVSLHPVGSVEPDA+A
Subjt:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA

Query:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP
        FF+PDE                    EHAATVLQYFLG Q KAALYPLLLWICSYQNLFSKACSKCGR LSVNKQSDLLLPPA+RPYKQFC S+  S VP
Subjt:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP

Query:  ISSLKDQRSDPVQAFHIGCFSEEL
        I+SLKDQ+ D VQAFHI CFSEE+
Subjt:  ISSLKDQRSDPVQAFHIGCFSEEL

A0A1S3B7L6 mediator of RNA polymerase II transcription subunit 27

2.38e-242

81.37

Show/hide

Query:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ
        MR  QQP+QGQTATVSPNT   HS  SA++APPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTA PHQS+KPLHLFQKED SMRQHL DLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL+DE+  N EPALKKPCFSHGVT++KQ+EL 
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN

Query:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA
        + R+LSDIL+C+EKEVP+LKIFTYERLDWLKQA AL SQAN+NSIGALKEH+YHSPRM PG+L A+  DK  VIELIIPSVFRAVVSLHP+GSVEPDA+A
Subjt:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA

Query:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP
        FF+PDE                    EHAATVLQYFLG Q KAALYPLLLWICSYQNLFSKACSKCGR LSVNKQSDLLLPP +RPYKQFC S+  SAVP
Subjt:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP

Query:  ISSLKDQRSDPVQAFHIGCFSEEL
        I+SLKDQ+ D VQAFHI CFSEE+
Subjt:  ISSLKDQRSDPVQAFHIGCFSEEL

A0A6J1DC62 mediator of RNA polymerase II transcription subunit 27

2.14e-287

95.28

Show/hide

Query:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ
        MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN

Query:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA
        EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA
Subjt:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA

Query:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP
        FFAPDE                    EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP
Subjt:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP

Query:  ISSLKDQRSDPVQAFHIGCFSEEL
        ISSLKDQRSDPVQAFHIGCFSEEL
Subjt:  ISSLKDQRSDPVQAFHIGCFSEEL

A0A6J1FP59 mediator of RNA polymerase II transcription subunit 27

7.59e-251

84.12

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Query:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ
        MRQTQ PAQG TATVSP T   HS GSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTA PHQSTKPLHLFQKED SMRQHL DLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLD+E+  N+EPA KKPCFSHGVT++KQ+ELN
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN

Query:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA
        +  +LSDILTCMEKEVPDLKIFTYERLDWLKQA AL SQAN+NSIGALKEHNYHSPRM P SL A+S DK +VIELIIPSVFRAVVSLHPVGSVEPDAVA
Subjt:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA

Query:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP
        FF+PDE                    EHAATVLQYF+GIQ KAAL+PLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTS++ S VP
Subjt:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP

Query:  ISSLKDQRSDPVQAFHIGCFSE
        ++SLKDQ+ D VQAFHIGCF+E
Subjt:  ISSLKDQRSDPVQAFHIGCFSE

A0A6J1KZZ9 mediator of RNA polymerase II transcription subunit 27

3.09e-250

83.89

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Query:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ
        MRQTQ PAQG TATVSP T   HS GSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTA PHQSTKPLHLFQKED SMRQHL DLRAVGKQ
Subjt:  MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLD+E+  N+EPA KKPCFSHGVT++KQ+ELN
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELN

Query:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA
        +  +LSDILTCMEKEVPDLKIFTYERLDWLKQA  L SQAN+NSIG LKEHNYHSPRM P SL A+S DK +VIELIIPSVFRAVVSLHPVGSVEPDAVA
Subjt:  EPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA

Query:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP
        FF+PDE                    EHAATVLQYF+GIQ KAAL+PLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTS++ SAVP
Subjt:  FFAPDE--------------------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP

Query:  ISSLKDQRSDPVQAFHIGCFSE
        ++SLKDQ+ D VQAFHIGCF+E
Subjt:  ISSLKDQRSDPVQAFHIGCFSE

SwissProt top hits

e value

%identity

Alignment

Q8RWM3 Mediator of RNA polymerase II transcription subunit 27

1.2e-110

54.93

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Query:  NTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQLEESGVLSESLLSRSNS
        N A   +  S  +APPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V + P  +  PL LF +ED SMRQHL+DLR +GK+LEESGVL+ESL SRSNS
Subjt:  NTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQLEESGVLSESLLSRSNS

Query:  WGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELNEPRTLSDILTCMEKEVP
        WGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPH+DD   L +EP+ KK   SH + E  ++E  + +TL DI + +EK VP
Subjt:  WGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELNEPRTLSDILTCMEKEVP

Query:  DLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVAFFAPDE-----------
         +K+ TY RL+WLK+A +L    +D+   A K     S ++  G L     DK++VIEL  PS+FRA+VSL P GSV+PDAVAFF+PDE           
Subjt:  DLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVAFFAPDE-----------

Query:  ---------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVPISSLKDQRSDPVQAFHI
                 EHAAT LQYFLG     ALY LLLWICS++++FSK C+KCGRLL+++K+S L+LPP  R Y++         +P++       D  +A+H 
Subjt:  ---------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVPISSLKDQRSDPVQAFHI

Query:  GCFSEE
         C  ++
Subjt:  GCFSEE

Arabidopsis top hits

e value

%identity

Alignment

AT3G09180.1 CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med27 (InterPro:IPR021627); Has 112 Blast hits to 112 proteins in 38 species: Archae - 0; Bacteria - 0; Metazoa - 79; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink).

8.8e-112

54.93

Show/hide

Query:  NTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQLEESGVLSESLLSRSNS
        N A   +  S  +APPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V + P  +  PL LF +ED SMRQHL+DLR +GK+LEESGVL+ESL SRSNS
Subjt:  NTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQLEESGVLSESLLSRSNS

Query:  WGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELNEPRTLSDILTCMEKEVP
        WGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPH+DD   L +EP+ KK   SH + E  ++E  + +TL DI + +EK VP
Subjt:  WGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELNEPRTLSDILTCMEKEVP

Query:  DLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVAFFAPDE-----------
         +K+ TY RL+WLK+A +L    +D+   A K     S ++  G L     DK++VIEL  PS+FRA+VSL P GSV+PDAVAFF+PDE           
Subjt:  DLKIFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVAFFAPDE-----------

Query:  ---------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVPISSLKDQRSDPVQAFHI
                 EHAAT LQYFLG     ALY LLLWICS++++FSK C+KCGRLL+++K+S L+LPP  R Y++         +P++       D  +A+H 
Subjt:  ---------EHAATVLQYFLGIQEKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVPISSLKDQRSDPVQAFHI

Query:  GCFSEE
         C  ++
Subjt:  GCFSEE

Sequences

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CDS sequence

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ATGCGCCAGACGCAGCAGCCAGCGCAGGGCCAGACTGCGACGGTGAGCCCTAACACTGCTGCGCCTCACTCCGGCGGCTCTGCAGAGGAGGCGCCGCCGAAGCAGGTGGC
ACTCGCCATGGATAGACTCGGCCACGCCGCCCGTCTCATCGCCGACATCAGGCTCGGCGCCGATCGCCTTCTCGAAGCCCTATGCGTCACCGCGCACCCTCACCAGAGCA
CCAAGCCTCTCCACTTGTTTCAGAAAGAAGACGATTCGATGCGCCAACACCTCCGAGACCTTCGCGCAGTTGGAAAGCAGCTCGAAGAGTCTGGTGTTCTCAGTGAATCT
CTTCTTTCACGAAGTAATTCTTGGGGTCTACATATGCCTTTAGTGTGTCCTGACGGTGCAGTGGTTGCTTATGCTTGGAAACGTCAACTGGCTGGTCAGGCGGGTGCATC
TGCAGTTGACCGGACGAGGTTAGCTCTTAAGGCCTTCACTGACCAGAAAAGACGGTTCTTCCCTCATCTTGATGATGAAAGCAGGTTGAATCTTGAGCCTGCTCTGAAGA
AGCCTTGTTTTTCTCACGGAGTGACAGAGATTAAACAAGACGAGCTTAATGAACCTAGAACACTTTCAGATATTCTAACATGTATGGAAAAGGAAGTGCCAGATCTTAAG
ATTTTCACTTACGAGCGATTAGACTGGTTGAAACAAGCTCCAGCACTTTCCTCACAAGCAAATGATAATTCGATCGGAGCACTAAAAGAACATAACTATCATAGTCCTAG
GATGGGCCCTGGATCACTTGCTGCCAGTTCCAAAGATAAAGTTTCTGTGATTGAGTTGATTATTCCTTCTGTCTTTAGGGCCGTAGTATCACTGCATCCAGTTGGTTCCG
TAGAACCAGATGCTGTAGCTTTCTTTGCTCCTGATGAGGAGCATGCTGCCACTGTGCTGCAGTATTTTCTTGGAATTCAAGAAAAAGCAGCTCTATATCCTCTATTGCTT
TGGATCTGCAGCTACCAAAATTTATTTTCAAAAGCTTGTAGTAAATGCGGACGGCTACTTTCTGTTAATAAACAATCAGATTTATTGTTACCTCCTGCCATTCGCCCTTA
CAAGCAGTTTTGTACATCAAGAGTCTTATCAGCTGTGCCCATATCCTCACTGAAAGACCAGAGGTCTGATCCTGTTCAAGCTTTTCACATCGGTTGCTTCTCAGAAGAAC
TA

mRNA sequence

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Protein sequence

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MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQLEESGVLSES
LLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEPALKKPCFSHGVTEIKQDELNEPRTLSDILTCMEKEVPDLK
IFTYERLDWLKQAPALSSQANDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVAFFAPDEEHAATVLQYFLGIQEKAALYPLLL
WICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVPISSLKDQRSDPVQAFHIGCFSEEL

MC05g1222 (gene) of Bitter gourd (Dali-11) v1 genome