CuGenDBv2

Gene ID	MS002077
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	protein MIS12 homolog
Genome location	scaffold30:2229421..2231914
RNA-Seq Expression	MS002077
Synteny	MS002077
Gene Ontology terms	GO:0000070 - mitotic sister chromatid segregation (biological process) GO:0034501 - protein localization to kinetochore (biological process) GO:0051301 - cell division (biological process) GO:0051382 - kinetochore assembly (biological process) GO:0000818 - nuclear MIS12/MIND complex (cellular component)
InterPro domains	IPR008685 - Centromere protein Mis12

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAG6589575.1 Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia]

7.6e-113

87.14

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Query:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN
        MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDLI+GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN

Query:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE
        DESP  TS+S DCLYDV LDTELD LRN+LSEVRKENVVLNQELQALERQTASSNSQ+N FNEAL+LYE+ SVNE FQE+M TASELRAKIGKLKKRR+E
Subjt:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE

Query:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL
        ESKLTKVEK+HTNGD+SHHHKGFSNAKLDDIQEFLADLK L
Subjt:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL

KAG7023263.1 Protein MIS12-like protein [Cucurbita argyrosperma subsp. argyrosperma]

4.9e-112

86.72

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Query:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN
        MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDLI+GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN

Query:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE
        DESP  TS+S DCLYDV LDTELD LRN+LSEVRKENVVLNQELQALERQ ASSNSQ+N FNEAL+LYE  SVNE FQE+M TASELRAKIGKLKKRR+E
Subjt:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE

Query:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL
        ESKLTKVEK+HTNGD+SHHHKGFSNAKLDDIQEFLADLK L
Subjt:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL

XP_022134693.1 protein MIS12 homolog [Momordica charantia]

4.2e-127

98.76

Show/hide

Query:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN
        MEGSKGEAVFDSLNLNP+LFINEALNLVDDLVDDAFDFYQ QASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN

Query:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE
        DESPADTSISHDCLYDVGLDTELDSLRNRLSEVR+ENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE
Subjt:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE

Query:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL
        ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL
Subjt:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL

XP_022921372.1 protein MIS12 homolog [Cucurbita moschata]

1.1e-111

86.31

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Query:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN
        MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDLI+GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN

Query:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE
        DESP  TS+S DCLYDV LDTELD LRN+LSEVRKEN+VLNQELQALERQ ASSNSQ+N FNEAL+LYE  SVNE FQE+M TASELRAKIGKLKKRR+E
Subjt:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE

Query:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL
        ESKLTKVEK+HTNGD SHHHKGFSNAKLDDIQEFLADLK L
Subjt:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL

XP_023515499.1 protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]

9.9e-113

87.14

Show/hide

Query:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN
        MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDL +GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN

Query:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE
        DESP  TS+S DCLYDV LDTELD LRN+LSEVRKENVVLNQELQALERQTASSNSQ+N FNEAL+LYE+ SVNE FQE+M TASELRAKIGKLKKRR+E
Subjt:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE

Query:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL
        ESKLTKVEK+HTNGD+SHHHKGFSNAKLDDIQEFLADLKTL
Subjt:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL

TrEMBL top hits

e value

%identity

Alignment

A0A5A7UQ95 Centromere protein Mis12

2.4e-104

81.74

Show/hide

Query:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN
        MEGSKGE+VF+SLNLNP+LFINEALN VDDLVDDAFDFYQSQASATLKTEG D+SQDL +GIS+VR+ V   LDKRLAMWEKYCLNHCF+VPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN

Query:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE
        DESP  TSI+HD   DV LDTELD LRN+LSEVRKEN+VLNQELQALERQTASSNSQI+HFNEAL+LYE++SVN+ FQE++ TASELR+KIGK+KKRR E
Subjt:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE

Query:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL
        E+KL KVEKVHTNGD+SHHH GFSNAKLDDIQEFL+DLK +
Subjt:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL

A0A6J1C2Q6 protein MIS12 homolog

2.0e-127

98.76

Show/hide

Query:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN
        MEGSKGEAVFDSLNLNP+LFINEALNLVDDLVDDAFDFYQ QASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN

Query:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE
        DESPADTSISHDCLYDVGLDTELDSLRNRLSEVR+ENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE
Subjt:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE

Query:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL
        ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL
Subjt:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL

A0A6J1E0A1 protein MIS12 homolog

5.3e-112

86.31

Show/hide

Query:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN
        MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDLI+GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN

Query:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE
        DESP  TS+S DCLYDV LDTELD LRN+LSEVRKEN+VLNQELQALERQ ASSNSQ+N FNEAL+LYE  SVNE FQE+M TASELRAKIGKLKKRR+E
Subjt:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE

Query:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL
        ESKLTKVEK+HTNGD SHHHKGFSNAKLDDIQEFLADLK L
Subjt:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL

A0A6J1JD43 protein MIS12 homolog isoform X1

2.2e-110

85.95

Show/hide

Query:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN
        MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDLI+GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN

Query:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQ-EVMNTASELRAKIGKLKKRRI
        DESP  TS+S DCLYDV LDTELD LR  LSEVRKEN+VLNQELQALERQTASSNSQ+N FNEAL+LYE+ SVNE FQ E+M TASELRAKIGKLKKRR+
Subjt:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQ-EVMNTASELRAKIGKLKKRRI

Query:  EESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL
        EESKLTKVEK+HTN D+SHHHKGFSNAKLDDIQEFLADLKTL
Subjt:  EESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL

A0A6J1JME3 protein MIS12 homolog isoform X2

9.1e-112

86.31

Show/hide

Query:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN
        MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDLI+GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSN

Query:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE
        DESP  TS+S DCLYDV LDTELD LR  LSEVRKEN+VLNQELQALERQTASSNSQ+N FNEAL+LYE+ SVNE FQE+M TASELRAKIGKLKKRR+E
Subjt:  DESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIE

Query:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL
        ESKLTKVEK+HTN D+SHHHKGFSNAKLDDIQEFLADLKTL
Subjt:  ESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL

SwissProt top hits

e value

%identity

Alignment

Q2V0Z5 Protein MIS12 homolog

2.8e-57

51.01

Show/hide

Query:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVD--KSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLP
        MEGSK EAVFDS+NLNP++FINEA+N V+D VD AFDFY   AS +LK +G D  KSQ L  GI+RVR  + SV+D RL +WE Y L  CFAVP+GF LP
Subjt:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVD--KSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLP

Query:  SNDESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRR
         ++ES   +S+  D LYD+ LD ELDSLR++L+ V K +V L+ ELQALER + S    +   NEALELY+E+S++E F+E+   ASELRA + +LK RR
Subjt:  SNDESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRR

Query:  IEESKLTKVEKVHTNGDMSHHHKGFS----NAKLDDIQEFLADLKTL
        ++ S+  KV++      + +H K FS    + KL+D+++F A+L+ +
Subjt:  IEESKLTKVEKVHTNGDMSHHHKGFS----NAKLDDIQEFLADLKTL

Arabidopsis top hits

e value

%identity

Alignment

AT5G35520.1 minichromosome instability 12 (mis12)-like

2.0e-58

51.01

Show/hide

Query:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVD--KSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLP
        MEGSK EAVFDS+NLNP++FINEA+N V+D VD AFDFY   AS +LK +G D  KSQ L  GI+RVR  + SV+D RL +WE Y L  CFAVP+GF LP
Subjt:  MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVD--KSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLP

Query:  SNDESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRR
         ++ES   +S+  D LYD+ LD ELDSLR++L+ V K +V L+ ELQALER + S    +   NEALELY+E+S++E F+E+   ASELRA + +LK RR
Subjt:  SNDESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRR

Query:  IEESKLTKVEKVHTNGDMSHHHKGFS----NAKLDDIQEFLADLKTL
        ++ S+  KV++      + +H K FS    + KL+D+++F A+L+ +
Subjt:  IEESKLTKVEKVHTNGDMSHHHKGFS----NAKLDDIQEFLADLKTL

Sequences

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CDS sequence

Show/hide CDS sequence

ATGGAAGGAAGTAAGGGCGAAGCGGTATTCGATTCACTGAATCTGAATCCACGGCTCTTCATTAATGAAGCTCTCAATCTTGTCGACGATTTGGTCGATGACGCTTTCGA
TTTTTATCAATCACAAGCATCAGCCACCTTGAAGACCGAGGGCGTGGATAAATCCCAGGATCTTATCATGGGCATATCGCGCGTCCGATCGTTCGTCCATTCAGTTTTGG
ATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTGAATCACTGTTTTGCGGTCCCTGAAGGCTTTTCTTTACCCTCAAATGATGAATCACCTGCCGACACTTCGATAAGT
CATGATTGCCTCTATGACGTGGGTCTGGATACAGAGTTAGATTCTTTGAGGAACAGGCTCTCTGAGGTCAGAAAAGAGAATGTTGTATTGAACCAAGAACTCCAAGCTTT
AGAAAGGCAAACTGCTTCCAGTAACTCACAAATCAATCATTTCAACGAGGCATTAGAATTATACGAGGAGACTTCAGTGAATGAGAGGTTCCAGGAAGTGATGAACACTG
CTTCGGAGCTCCGAGCGAAAATAGGAAAGCTGAAGAAAAGGAGGATAGAGGAATCCAAGCTCACTAAAGTAGAGAAGGTTCATACAAATGGCGACATGTCTCACCATCAC
AAGGGCTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTTTTAGCTGATTTGAAGACCCTT

mRNA sequence

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Protein sequence

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MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSIS
HDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHH
KGFSNAKLDDIQEFLADLKTL

MS002077 (gene) of Bitter gourd (TR) v1 genome