CuGenDBv2

Gene ID	MS005021
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Alanine--tRNA ligase
Genome location	scaffold176:2155658..2156798
RNA-Seq Expression	MS005021
Synteny	MS005021
Gene Ontology terms	GO:0006419 - alanyl-tRNA aminoacylation (biological process) GO:0006468 - protein phosphorylation (biological process) GO:0009742 - brassinosteroid mediated signaling pathway (biological process) GO:0005739 - mitochondrion (cellular component) GO:0005886 - plasma membrane (cellular component) GO:0009507 - chloroplast (cellular component) GO:0000049 - tRNA binding (molecular function) GO:0004672 - protein kinase activity (molecular function) GO:0004813 - alanine-tRNA ligase activity (molecular function) GO:0005524 - ATP binding (molecular function) GO:0008270 - zinc ion binding (molecular function)
InterPro domains	IPR018164 - Alanyl-tRNA synthetase, class IIc, N-terminal

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6604957.1 Alanine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia]

8.5e-62

84.67

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Query:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV
        MEF+LKLPNH P SYG  SLT L R+SLY   PAS A PRRKV  SSGYITRN APFPSTM   RHF+PNEARGTQLKTRTSVKPV EELVEDKSKDL V
Subjt:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV

Query:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
        +GDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
Subjt:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK

KAG7034991.1 Alanine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma]

8.5e-62

84.67

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Query:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV
        MEF+LKLPNH P SYG  SLT L R+SLY   PAS A PRRKV  SSGYITRN APFPSTM   RHF+PNEARGTQLKTRTSVKPV EELVEDKSKDL V
Subjt:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV

Query:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
        +GDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
Subjt:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK

XP_022140525.1 alanine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Momordica charantia]

2.1e-76

99.33

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Query:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV
        MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLP EARGTQLKTRTSVKPVNEELVEDKSKDLLV
Subjt:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV

Query:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
        TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
Subjt:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK

XP_022947694.1 alanine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita moschata]

1.0e-62

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Query:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV
        MEF+LKLPNH P SYG  SLT L R+SLY   PAS A PRRKV  SSGYITRN APFPSTM   RHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDL V
Subjt:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV

Query:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
        +GDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
Subjt:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK

XP_022970911.1 alanine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita maxima]

2.6e-63

86.67

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Query:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV
        MEF+LKLPNH P SYG  SLT L R+SLY   PAS A PRRKV  SSGYITRN APFPSTM   RHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDL V
Subjt:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV

Query:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
        TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
Subjt:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK

TrEMBL top hits

e value

%identity

Alignment

A0A1S3BK22 Probable alanine--tRNA ligase, chloroplastic

5.2e-57

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Query:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV
        MEFSLKLPNHLP ++   SLTSL  ++LY    AS APPRRK+M SSGY TRN A FPST   RR+FL  EARGTQLKT+TSVKPVNEELVEDKSKDL V
Subjt:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV

Query:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
        +GDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKP+FLGK
Subjt:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK

A0A5A7V333 Alanine--tRNA ligase

5.2e-57

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Query:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV
        MEFSLKLPNHLP ++   SLTSL  ++LY    AS APPRRK+M SSGY TRN A FPST   RR+FL  EARGTQLKT+TSVKPVNEELVEDKSKDL V
Subjt:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV

Query:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
        +GDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKP+FLGK
Subjt:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK

A0A6J1CFB3 Probable alanine--tRNA ligase, chloroplastic

1.0e-76

99.33

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Query:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV
        MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLP EARGTQLKTRTSVKPVNEELVEDKSKDLLV
Subjt:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV

Query:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
        TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
Subjt:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK

A0A6J1G7K6 Probable alanine--tRNA ligase, chloroplastic

4.8e-63

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Query:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV
        MEF+LKLPNH P SYG  SLT L R+SLY   PAS A PRRKV  SSGYITRN APFPSTM   RHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDL V
Subjt:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV

Query:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
        +GDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
Subjt:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK

A0A6J1I1V0 Probable alanine--tRNA ligase, chloroplastic

1.3e-63

86.67

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Query:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV
        MEF+LKLPNH P SYG  SLT L R+SLY   PAS A PRRKV  SSGYITRN APFPSTM   RHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDL V
Subjt:  MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLV

Query:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
        TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
Subjt:  TGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK

SwissProt top hits

e value

%identity

Alignment

B8B4H5 Alanine--tRNA ligase, chloroplastic/mitochondrial

9.2e-19

61.73

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Query:  RGTQLKTRT---SVKPVNEELVEDKSKDLLVTGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
        RG  +KT +   SV+   +E+    S +   +GD+IRRRFL FYA+RGHK+LPS+SLVP+DPTV LTIAGMLQFKPIFLGK
Subjt:  RGTQLKTRT---SVKPVNEELVEDKSKDLLVTGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK

B9FSH5 Alanine--tRNA ligase, chloroplastic/mitochondrial

9.2e-19

61.73

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Query:  RGTQLKTRT---SVKPVNEELVEDKSKDLLVTGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK
        RG  +KT +   SV+   +E+    S +   +GD+IRRRFL FYA+RGHK+LPS+SLVP+DPTV LTIAGMLQFKPIFLGK
Subjt:  RGTQLKTRT---SVKPVNEELVEDKSKDLLVTGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK

B9HQZ6 Alanine--tRNA ligase, chloroplastic/mitochondrial

3.4e-29

58.68

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Query:  PASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQL---KTRTSVKPVNEELVEDKSKDLLVTGDSIRRRFLQFYASRGHKVLPSASLVPE
        P+ +  P      S+G+ TRN A F  ++    +F+   AR  +     T+ SV+PV EELVEDK+K+  V+GD+IRRRFL+FYASR HKVLPSASLVP+
Subjt:  PASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQL---KTRTSVKPVNEELVEDKSKDLLVTGDSIRRRFLQFYASRGHKVLPSASLVPE

Query:  DPTVLLTIAGMLQFKPIFLGK
        DPTVLLTIAGMLQFKPIFLGK
Subjt:  DPTVLLTIAGMLQFKPIFLGK

C5Z7K4 Alanine--tRNA ligase, chloroplastic/mitochondrial

5.8e-21

56.6

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Query:  TRNPAPFPSTMQSRRHFL--PNEARGTQL----KTRTSVKPVNEELVEDKSKDLLVTGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFK
        +R  +P  ST  +RR  L  P    G +L    +TR SV+P  +EL    + +   +GD+IRRRFL+FYA+RGHK+LPS+SLVP+DPTVLLTIAGMLQFK
Subjt:  TRNPAPFPSTMQSRRHFL--PNEARGTQL----KTRTSVKPVNEELVEDKSKDLLVTGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFK

Query:  PIFLGK
        PIFLGK
Subjt:  PIFLGK

Q9FFC7 Alanine--tRNA ligase, chloroplastic/mitochondrial

7.3e-24

57.14

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Query:  VMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQL---KTRTSVKPVNEELVED-KSKDLLVTGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIA
        +  S+ ++TR  A    ++      LP   +  Q+    +  SV+PV+E+  ED +SKD  V+GDSIRRRFL+F+ASRGHKVLPS+SLVPEDPTVLLTIA
Subjt:  VMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQL---KTRTSVKPVNEELVED-KSKDLLVTGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIA

Query:  GMLQFKPIFLGK
        GMLQFKPIFLGK
Subjt:  GMLQFKPIFLGK

Arabidopsis top hits

e value

%identity

Alignment

AT1G50200.1 Alanyl-tRNA synthetase

8.3e-07

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Query:  IRRRFLQFYASRGHKVLPSASLVP-EDPTVLLTIAGMLQFKPIFLG
        +R  ++ F+  +GHK  PS+ +VP  DPT+L   AGM Q+KPIFLG
Subjt:  IRRRFLQFYASRGHKVLPSASLVP-EDPTVLLTIAGMLQFKPIFLG

AT1G50200.2 Alanyl-tRNA synthetase

8.3e-07

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Query:  IRRRFLQFYASRGHKVLPSASLVP-EDPTVLLTIAGMLQFKPIFLG
        +R  ++ F+  +GHK  PS+ +VP  DPT+L   AGM Q+KPIFLG
Subjt:  IRRRFLQFYASRGHKVLPSASLVP-EDPTVLLTIAGMLQFKPIFLG

AT5G22800.1 Alanyl-tRNA synthetase, class IIc

5.2e-25

57.14

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Query:  VMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQL---KTRTSVKPVNEELVED-KSKDLLVTGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIA
        +  S+ ++TR  A    ++      LP   +  Q+    +  SV+PV+E+  ED +SKD  V+GDSIRRRFL+F+ASRGHKVLPS+SLVPEDPTVLLTIA
Subjt:  VMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQL---KTRTSVKPVNEELVED-KSKDLLVTGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIA

Query:  GMLQFKPIFLGK
        GMLQFKPIFLGK
Subjt:  GMLQFKPIFLGK

Sequences

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CDS sequence

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ATGGAATTCAGCCTGAAGCTCCCAAATCATCTCCCTTGTAGTTATGGCGACAAATCTCTTACTTCCCTTTCACGTGCAAGCTTATACCGCAAAAATCCTGCCTCCTCTGC
TCCACCCAGGCGGAAAGTTATGTCTTCTTCCGGCTATATTACAAGAAATCCGGCCCCATTTCCTTCCACCATGCAGTCCCGCAGACATTTCCTTCCAAATGAAGCTCGGG
GCACACAACTCAAAACAAGAACATCTGTGAAACCTGTGAATGAGGAATTAGTAGAGGACAAGTCCAAGGATCTCCTAGTGACAGGCGATTCTATTCGTCGTCGGTTTCTT
CAATTCTATGCTTCCCGTGGACACAAAGTTCTTCCCAGTGCTTCTTTGGTACCAGAAGATCCCACGGTATTATTGACAATTGCAGGAATGCTTCAATTCAAGCCTATCTT
CCTTGGAAAG

mRNA sequence

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Protein sequence

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MEFSLKLPNHLPCSYGDKSLTSLSRASLYRKNPASSAPPRRKVMSSSGYITRNPAPFPSTMQSRRHFLPNEARGTQLKTRTSVKPVNEELVEDKSKDLLVTGDSIRRRFL
QFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGK

MS005021 (gene) of Bitter gourd (TR) v1 genome