CuGenDBv2

Gene ID	MS008516
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	DNA-directed RNA polymerase subunit beta'
Genome location	scaffold4:1937532..1937749
RNA-Seq Expression	MS008516
Synteny	MS008516
Gene Ontology terms	GO:0006351 - transcription, DNA-templated (biological process) GO:0009507 - chloroplast (cellular component) GO:0000287 - magnesium ion binding (molecular function) GO:0003677 - DNA binding (molecular function) GO:0003899 - DNA-directed 5'-3' RNA polymerase activity (molecular function) GO:0008270 - zinc ion binding (molecular function)
InterPro domains	IPR000722 - RNA polymerase, alpha subunit IPR042102 - RNA polymerase Rpb1, domain 3 superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

QAU08817.1 RpoC1, partial [Triplostegia grandiflora]

1.0e-25

87.67

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE QAEARLLMFSHMNLLS AIGNPISVPTQDMLIGLYVL  GNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

QXO90323.1 RNA polymerase beta' subunit [Tara cacalaco]

1.0e-25

87.67

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE QAEARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SGNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

QZJ45910.1 RNA polymerase beta' subunit [Mazus omeiensis]

1.0e-25

87.67

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE QAEARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SGNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

YP_009663468.1 RpoC1 [Ipomoea setifera]

1.0e-25

87.67

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE QAEARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SGNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

YP_009939302.1 RNA polymerase beta' subunit [Salvia plebeia]

1.0e-25

87.67

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE QAEARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SGNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

TrEMBL top hits

e value

%identity

Alignment

A0A411JXK4 DNA-directed RNA polymerase subunit gamma

5.1e-26

87.67

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE QAEARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SGNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

A0A4P9JKS1 DNA-directed RNA polymerase subunit beta'

5.1e-26

87.67

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE QAEARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SGNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

A0A7G8JTA5 DNA-directed RNA polymerase subunit beta'

5.1e-26

87.67

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE QAEARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SGNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

A0A7L7S1Z1 DNA-directed RNA polymerase subunit beta'

5.1e-26

87.67

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE QAEARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SGNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

A0A7M1CGA3 DNA-directed RNA polymerase subunit beta'

5.1e-26

87.67

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE QAEARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SGNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

SwissProt top hits

e value

%identity

Alignment

P12116 DNA-directed RNA polymerase subunit beta'

4.0e-28

86.3

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE Q EARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SGNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

Q2MIA9 DNA-directed RNA polymerase subunit beta'

4.0e-28

86.3

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE Q EARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SGNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

Q2MIJ6 DNA-directed RNA polymerase subunit beta'

4.0e-28

86.3

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE Q EARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SGNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

Q3C1G8 DNA-directed RNA polymerase subunit beta'

4.0e-28

86.3

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH
        +I +H LVCKGFNADFD DQMAVHVPLSLE Q EARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SGNH
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

Q4VZP2 DNA-directed RNA polymerase subunit beta'

4.0e-28

88.89

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGN
        +I +H LVCKGFNADFD DQMAVHVPLSLE QAEARLLMFSHMNLLSSAIG+PISVPTQDMLIGLYVL SGN
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGN

Arabidopsis top hits

e value

%identity

Alignment

AT4G35800.1 RNA polymerase II large subunit

1.9e-04

42.11

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Query:  VCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIG
        V   +NADFD D+M +HVP S ET+AE   LM     ++S     P+    QD L+G
Subjt:  VCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIG

ATCG00180.1 DNA-directed RNA polymerase family protein

5.3e-28

87.32

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Query:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSG
        +I +H LVCKGFNADFD DQMAVHVPLSLE QAEARLLMFSHMNLLS AIG+PISVPTQDMLIGLYVL SG
Subjt:  SIDIHQLVCKGFNADFD-DQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSG

Sequences

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CDS sequence

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TCAATTGATATACATCAATTAGTTTGTAAGGGATTCAATGCAGACTTTGATGATCAAATGGCTGTTCATGTACCTTTATCTTTGGAGACTCAAGCAGAGGCGCGTTTACT
CATGTTTTCTCATATGAATCTCTTGTCTTCAGCTATTGGGAATCCCATTTCTGTACCAACTCAAGATATGCTTATTGGGCTCTATGTATTAATGAGCGGGAATCAC

mRNA sequence

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Protein sequence

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SIDIHQLVCKGFNADFDDQMAVHVPLSLETQAEARLLMFSHMNLLSSAIGNPISVPTQDMLIGLYVLMSGNH

MS008516 (gene) of Bitter gourd (TR) v1 genome