CuGenDBv2

Gene ID	MS012425
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	mediator of RNA polymerase II transcription subunit 28
Genome location	scaffold63:600116..601986
RNA-Seq Expression	MS012425
Synteny	MS012425
Gene Ontology terms	GO:0006355 - regulation of transcription, DNA-templated (biological process) GO:0016592 - mediator complex (cellular component)
InterPro domains	IPR034456 - Mediator of RNA polymerase II transcription subunit 28

Homology

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GenBank top hits

e value

%identity

Alignment

XP_004152267.1 mediator of RNA polymerase II transcription subunit 28 [Cucumis sativus]

1.5e-63

92.2

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Query:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS
        MA+RQP+DQQH LDPQLQS QSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ+DVERHARDFMEAAKKLQLYFIGLQREDQP KVETLRKEIS
Subjt:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS

Query:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
        AIEEELK+KN+IIKK E+LIEGWKKDLKDQL KHNNELEKV
Subjt:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV

XP_008454378.1 PREDICTED: mediator of RNA polymerase II transcription subunit 28 [Cucumis melo]

2.1e-62

91.49

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Query:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS
        MA+RQ +DQQH LDPQ QS QSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ+DVERHARDFMEAAKKLQLYFIGLQREDQP KVETLRKEIS
Subjt:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS

Query:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
        AIEEELK+KNDIIKK ERLIEGWKKDLK+QL KHNNELEKV
Subjt:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV

XP_022149222.1 mediator of RNA polymerase II transcription subunit 28 [Momordica charantia]

1.8e-69

100

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Query:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS
        MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS
Subjt:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS

Query:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
        AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
Subjt:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV

XP_022922953.1 mediator of RNA polymerase II transcription subunit 28 [Cucurbita moschata]

1.9e-63

92.2

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Query:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS
        MA+RQP+DQQ+Q+DPQLQS QSPREDM++YVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQP KVETLRKEIS
Subjt:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS

Query:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
        AIEEELKLK+DIIKK ERLIEGWKKDLKDQL KHNNELEKV
Subjt:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV

XP_038904970.1 mediator of RNA polymerase II transcription subunit 28 [Benincasa hispida]

5.0e-64

93.62

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Query:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS
        MA+RQP+DQQH LDPQLQS QSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ+DVERHARDFMEAAKKLQLYFIGLQREDQP KVETLRKEIS
Subjt:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS

Query:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
        AIEEELKLKN+IIKK ERLIEGWKKDLKDQL KHNNELEKV
Subjt:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KT19 Uncharacterized protein

7.0e-64

92.2

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Query:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS
        MA+RQP+DQQH LDPQLQS QSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ+DVERHARDFMEAAKKLQLYFIGLQREDQP KVETLRKEIS
Subjt:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS

Query:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
        AIEEELK+KN+IIKK E+LIEGWKKDLKDQL KHNNELEKV
Subjt:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV

A0A1S3BZ89 mediator of RNA polymerase II transcription subunit 28

1.0e-62

91.49

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Query:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS
        MA+RQ +DQQH LDPQ QS QSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ+DVERHARDFMEAAKKLQLYFIGLQREDQP KVETLRKEIS
Subjt:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS

Query:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
        AIEEELK+KNDIIKK ERLIEGWKKDLK+QL KHNNELEKV
Subjt:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV

A0A6J1D7C5 mediator of RNA polymerase II transcription subunit 28

8.6e-70

100

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Query:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS
        MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS
Subjt:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS

Query:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
        AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
Subjt:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV

A0A6J1E897 mediator of RNA polymerase II transcription subunit 28

9.2e-64

92.2

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Query:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS
        MA+RQP+DQQ+Q+DPQLQS QSPREDM++YVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQP KVETLRKEIS
Subjt:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS

Query:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
        AIEEELKLK+DIIKK ERLIEGWKKDLKDQL KHNNELEKV
Subjt:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV

A0A6J1JAT8 mediator of RNA polymerase II transcription subunit 28

9.2e-64

92.2

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Query:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS
        MA+RQP+DQQ+Q+DPQLQS QSPREDM++YVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQP KVETLRKEIS
Subjt:  MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEIS

Query:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
        AIEEELKLK+DIIKK ERLIEGWKKDLKDQL KHNNELEKV
Subjt:  AIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV

SwissProt top hits

e value

%identity

Alignment

Q9LFA5 Mediator of RNA polymerase II transcription subunit 28

6.8e-40

65.19

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Query:  PQLQSPQSPR-------EDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQ-PIKVETLRKEISAIEEEL
        P ++SP++P        ED++A V ALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYF+GL+RED+ P + E+L+K+I+ +EEEL
Subjt:  PQLQSPQSPR-------EDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQ-PIKVETLRKEISAIEEEL

Query:  KLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
        K K+++IKKH RL +  +K +K+Q+ KH +ELEKV
Subjt:  KLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV

Arabidopsis top hits

e value

%identity

Alignment

AT3G52860.1 unknown protein

4.8e-41

65.19

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Query:  PQLQSPQSPR-------EDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQ-PIKVETLRKEISAIEEEL
        P ++SP++P        ED++A V ALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYF+GL+RED+ P + E+L+K+I+ +EEEL
Subjt:  PQLQSPQSPR-------EDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQ-PIKVETLRKEISAIEEEL

Query:  KLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV
        K K+++IKKH RL +  +K +K+Q+ KH +ELEKV
Subjt:  KLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV

Sequences

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CDS sequence

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ATGGCTGATCGACAACCAATGGATCAACAGCATCAACTTGATCCACAGTTGCAATCCCCGCAGTCACCGAGAGAGGACATGATTGCATATGTGATGGCCTTGGAAGCTGC
TCTGCTTCCGTGCTTGCCTGCAAGAGAGTTACAAGCAATAGACCGATCTCCTCACCCTTCTCATCAAATTGACGTGGAGAGACATGCTAGGGATTTTATGGAGGCAGCTA
AAAAGCTTCAACTGTACTTCATTGGTTTACAACGTGAGGATCAACCAATCAAAGTGGAAACACTAAGAAAGGAGATATCCGCCATTGAGGAGGAACTAAAATTAAAGAAT
GACATTATCAAGAAGCATGAGAGGCTCATTGAAGGGTGGAAGAAGGATTTGAAAGACCAGTTAGCCAAACATAACAATGAATTAGAGAAGGTT

mRNA sequence

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Protein sequence

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MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEISAIEEELKLKN
DIIKKHERLIEGWKKDLKDQLAKHNNELEKV

MS012425 (gene) of Bitter gourd (TR) v1 genome