CuGenDBv2

Gene ID	MS015457
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Threonylcarbamoyladenosine tRNA methylthiotransferase
Genome location	scaffold424:230515..242019
RNA-Seq Expression	MS015457
Synteny	MS015457
Gene Ontology terms	GO:0035600 - tRNA methylthiolation (biological process) GO:0005783 - endoplasmic reticulum (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0035598 - N6-threonylcarbomyladenosine methylthiotransferase activity (molecular function) GO:0046872 - metal ion binding (molecular function) GO:0051539 - 4 iron, 4 sulfur cluster binding (molecular function) GO:0061712 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase (molecular function)
InterPro domains	IPR002792 - TRAM domain IPR005839 - Methylthiotransferase IPR006466 - MiaB-like tRNA modifying enzyme, archaeal-type IPR006638 - Elp3/MiaB/NifB IPR007197 - Radical SAM IPR013848 - Methylthiotransferase, N-terminal IPR020612 - Methylthiotransferase, conserved site IPR023404 - Radical SAM, alpha/beta horseshoe IPR038135 - Methylthiotransferase, N-terminal domain superfamily

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

XP_022155234.1 threonylcarbamoyladenosine tRNA methylthiotransferase [Momordica charantia]

0.0e+00

94.9

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Query:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP
        MEDIEDLLIGGGGGGGAPPGYRLPLTAVG+KPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT             QSDSEYMAGQLSAFGYLLSDNP
Subjt:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP

Query:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI
        EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE                VRKNKFI
Subjt:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI

Query:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK
        EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Subjt:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK

Query:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA
        EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA
Subjt:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA

Query:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT
        RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVK+TSVGRWSVFGDVIETISTRHHKTT
Subjt:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT

Query:  TLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASGEWGFV
        TLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWL RRRKRHVAKREERESSGIASERKQASGEWGFV
Subjt:  TLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASGEWGFV

Query:  DRILVGGILISIFAIVGVLFHLGSTTL
        DRILVGGILISIFAIVGVLFHLGSTTL
Subjt:  DRILVGGILISIFAIVGVLFHLGSTTL

XP_022949069.1 threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita moschata]

7.5e-303

85.65

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Query:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP
        MEDIEDLLI GGGGGGAPPGYRLP++AVG+KPKKKNKLKSNPDADDSS+ L PTQN L PKIPGT             QSDSEYMAGQLSAFGYLLS+NP
Subjt:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP

Query:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI
        EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE                VRKNKFI
Subjt:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI

Query:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK
        EILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVRTVINEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Subjt:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK

Query:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA
        EIANVLCHPCVYSFLHVPVQSGSD ILSAMNREYTV EFRTVVDTLTELVPGMQ+ATDIICGFPGETDEDFC+TINLIKEYKLPQVHISQFYPRPGTPAA
Subjt:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA

Query:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT
        RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVI  ETMLGTSA+VKITSVGRWSVFG+VIETI T+HHKTT
Subjt:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT

Query:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPES-CKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASG---
         LE+MPIQ KV PC + HE C+ S EPE CACGPES CK PV LD KDDSS NI L EEPKRKN++EWLLR+RK H  KREERES  + SERKQAS    
Subjt:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPES-CKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASG---

Query:  --EWGFVDRILVGGILISIFAIVGVLFHLGSTTL
          EWG VDRILVGGILISIF IVG+LFHLGSTTL
Subjt:  --EWGFVDRILVGGILISIFAIVGVLFHLGSTTL

XP_022997808.1 threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita maxima]

2.8e-302

85.33

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Query:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP
        MEDIEDLLIGGGGGGGAPPGYRLP++AVG+K KKKNKLK NPDADDSS+ L PTQN L PKIPGT             QSDSEYMAGQLSAFGYLLS+NP
Subjt:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP

Query:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI
        EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE                VRKNKFI
Subjt:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI

Query:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK
        EILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVRTVINEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Subjt:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK

Query:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA
        EIANVLCHPCVYSFLHVPVQSGSD ILSAMNREYTV EFRTVVDTLTELVPGMQ+ATDIICGFPGETDEDFC+TINLIKEYKLPQVHISQFYPRPGTPAA
Subjt:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA

Query:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT
        RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVI  E MLGTSA+VKITSVGRWSVFG+VIETI T+HHKTT
Subjt:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT

Query:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPES-CKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQAS----
         LE+MPIQ KV PC N+HE C+ S EPE CACGPES CK PV LD KDDSS NI L EEPKRKN++EWLLR+RK HV KREE ES  + S RKQAS    
Subjt:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPES-CKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQAS----

Query:  -GEWGFVDRILVGGILISIFAIVGVLFHLGSTTL
          EWG VDRILVGGILISIF IVG+LFHLGSTTL
Subjt:  -GEWGFVDRILVGGILISIFAIVGVLFHLGSTTL

XP_023524910.1 threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita pepo subsp. pepo]

1.4e-301

85.33

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Query:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP
        MEDIEDLLI   GGGGAPPGYRLP++AVG+KPKKKNKLK NPDADDSS+ L PTQN L PKIPGT             QSDSEYMAGQLSAFGYLLS+NP
Subjt:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP

Query:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI
        EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE                VRKNKFI
Subjt:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI

Query:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK
        EILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVRTVINEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Subjt:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK

Query:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA
        EIANVLCHPCVYSFLHVPVQSGSD ILSAMNREYTV EFRTVVDTLTELVPGMQ+ATDIICGFPGETDEDFC+TINLIKEYKLPQVHISQFYPRPGTPAA
Subjt:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA

Query:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT
        RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVI PE MLGTSA+VKITSVGRWSVFG+VIETI T+HHKTT
Subjt:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT

Query:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPES-CKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQAS----
         LE+MPIQ KV PC N HE C+ S EPE CACG ES CK PV LD KDDSS NI L EEPKRKN++EWLLR+RK HV KREERES  + SERKQAS    
Subjt:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPES-CKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQAS----

Query:  -GEWGFVDRILVGGILISIFAIVGVLFHLGSTTL
          EWG VDRILVGGILISIF IVG+LFHLGSTTL
Subjt:  -GEWGFVDRILVGGILISIFAIVGVLFHLGSTTL

XP_038890156.1 threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Benincasa hispida]

2.2e-302

85.31

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Query:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP
        MEDIEDLLI   GGGGAPPGYRLP+ AVG+KPKKKN LK NP+ADDSSN+LSPTQNPLVPKIPGT             QSDSEYMAGQLSAFGYLLSDNP
Subjt:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP

Query:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI
        E+ADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE                VRKNKFI
Subjt:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI

Query:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK
        EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVR+VINEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Subjt:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK

Query:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA
        EIANVL HPCVYSFLHVPVQSGSD ILSAMNREYTV EFRTVVDTLTELVPGMQ+ATDIICGFPGETDEDFC+TINLIKEYKLPQVHISQFYPRPGTPAA
Subjt:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA

Query:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT
        RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITS GRWSVFG+VIETIST+HHKTT
Subjt:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT

Query:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASG----
        TLE+ PI+DKV PC N HE C+ S EPE CACGPESCK  V  D+KDDSS NI L EEPKRKN++EW LRRRK HV KREERES  I SERKQA      
Subjt:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASG----

Query:  -EWGFVDRILVGGILISIFAIVGVLFHLGSTTL
         EWG +DRILVGGILIS   I G+LFHLGSTTL
Subjt:  -EWGFVDRILVGGILISIFAIVGVLFHLGSTTL

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KSZ4 Threonylcarbamoyladenosine tRNA methylthiotransferase

1.4e-291

82.75

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Query:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP
        MEDIEDLLI  GGGGGAPPGYRLP+TAVG+KPKKKN  KSN  ADDSSN+ SP QNPLVPKIPGT             QSDSEYMAGQLSAFGYLLSDNP
Subjt:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP

Query:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI
        E+ADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE                VRKNKFI
Subjt:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI

Query:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK
        EILPINVGCLGACTYCKTKHARGHLGSYTVDSLV+RVR+VINEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIV+ELP DASTMLRIGMTNPPFILEHLK
Subjt:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK

Query:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA
        EIA VL HPCVYSFLHVPVQSGSDAILSAMNREYTV EFRTVVDTLTELVPGMQIATDIICGFPGETDEDFC+TINLIKEY LPQVHISQFYPRPGTPAA
Subjt:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA

Query:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT
        RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSA VKITS+GRWSVFG+VIE IST+HHKTT
Subjt:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT

Query:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQ--ASG--
        TLE+   QDKV PC NTHE C+ STEPE CACG ESCK  V + ++ +SS N+   EEPKRKN+IEW+LRRRK HV  + E   + I +ERKQ  A G  
Subjt:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQ--ASG--

Query:  -EWGFVDRILVGGILISIFAIVGVLFHLGSTT
         EWG VD+ILVGGILIS F I G+LFHLGSTT
Subjt:  -EWGFVDRILVGGILISIFAIVGVLFHLGSTT

A0A1S3CA89 Threonylcarbamoyladenosine tRNA methylthiotransferase

2.4e-291

83.25

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Query:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP
        MEDIEDLLI   GGGGAPPGYRLP+TAVG+KPKKKN  KSNP ADDSSNQ SPTQNPLVPKIPGT             QSDSEYMAGQLSAFGYLLSDNP
Subjt:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP

Query:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI
        E+ADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE                VRKNKFI
Subjt:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI

Query:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK
        EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVR+VINEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Subjt:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK

Query:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA
        EIA VL HPCVYSFLHVPVQSGSDAILSAMNREYTV EFRTVVDTLTELVPGMQIATDIICGFPGETDEDFC+TINLIKEY LPQVHISQFYPRPGTPAA
Subjt:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA

Query:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT
        RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAM KITS+GRWSVFG+VIETIST+HHK T
Subjt:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT

Query:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQ--ASG--
        TLE+   +DKV PC NTHE C+ STEPE CAC   SCK  V++ ++ DSS NI L EEPKRKN+IEW+LRRRK  V  + E   + I +ERKQ  A G  
Subjt:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQ--ASG--

Query:  -EWGFVDRILVGGILISIFAIVGVLFHLGSTTL
         EWG VD ILVGGILIS F I G+LFH+GSTTL
Subjt:  -EWGFVDRILVGGILISIFAIVGVLFHLGSTTL

A0A6J1DPN3 Threonylcarbamoyladenosine tRNA methylthiotransferase

0.0e+00

94.9

Show/hide

Query:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP
        MEDIEDLLIGGGGGGGAPPGYRLPLTAVG+KPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT             QSDSEYMAGQLSAFGYLLSDNP
Subjt:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP

Query:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI
        EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE                VRKNKFI
Subjt:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI

Query:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK
        EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Subjt:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK

Query:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA
        EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA
Subjt:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA

Query:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT
        RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVK+TSVGRWSVFGDVIETISTRHHKTT
Subjt:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT

Query:  TLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASGEWGFV
        TLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWL RRRKRHVAKREERESSGIASERKQASGEWGFV
Subjt:  TLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASGEWGFV

Query:  DRILVGGILISIFAIVGVLFHLGSTTL
        DRILVGGILISIFAIVGVLFHLGSTTL
Subjt:  DRILVGGILISIFAIVGVLFHLGSTTL

A0A6J1GBS1 Threonylcarbamoyladenosine tRNA methylthiotransferase

3.6e-303

85.65

Show/hide

Query:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP
        MEDIEDLLI GGGGGGAPPGYRLP++AVG+KPKKKNKLKSNPDADDSS+ L PTQN L PKIPGT             QSDSEYMAGQLSAFGYLLS+NP
Subjt:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP

Query:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI
        EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE                VRKNKFI
Subjt:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI

Query:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK
        EILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVRTVINEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Subjt:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK

Query:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA
        EIANVLCHPCVYSFLHVPVQSGSD ILSAMNREYTV EFRTVVDTLTELVPGMQ+ATDIICGFPGETDEDFC+TINLIKEYKLPQVHISQFYPRPGTPAA
Subjt:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA

Query:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT
        RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVI  ETMLGTSA+VKITSVGRWSVFG+VIETI T+HHKTT
Subjt:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT

Query:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPES-CKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASG---
         LE+MPIQ KV PC + HE C+ S EPE CACGPES CK PV LD KDDSS NI L EEPKRKN++EWLLR+RK H  KREERES  + SERKQAS    
Subjt:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPES-CKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASG---

Query:  --EWGFVDRILVGGILISIFAIVGVLFHLGSTTL
          EWG VDRILVGGILISIF IVG+LFHLGSTTL
Subjt:  --EWGFVDRILVGGILISIFAIVGVLFHLGSTTL

A0A6J1KEZ5 Threonylcarbamoyladenosine tRNA methylthiotransferase

1.4e-302

85.33

Show/hide

Query:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP
        MEDIEDLLIGGGGGGGAPPGYRLP++AVG+K KKKNKLK NPDADDSS+ L PTQN L PKIPGT             QSDSEYMAGQLSAFGYLLS+NP
Subjt:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSDNP

Query:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI
        EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE                VRKNKFI
Subjt:  EEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNKFI

Query:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK
        EILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVRTVINEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Subjt:  EILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK

Query:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA
        EIANVLCHPCVYSFLHVPVQSGSD ILSAMNREYTV EFRTVVDTLTELVPGMQ+ATDIICGFPGETDEDFC+TINLIKEYKLPQVHISQFYPRPGTPAA
Subjt:  EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAA

Query:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT
        RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVI  E MLGTSA+VKITSVGRWSVFG+VIETI T+HHKTT
Subjt:  RMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTT

Query:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPES-CKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQAS----
         LE+MPIQ KV PC N+HE C+ S EPE CACGPES CK PV LD KDDSS NI L EEPKRKN++EWLLR+RK HV KREE ES  + S RKQAS    
Subjt:  TLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPES-CKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQAS----

Query:  -GEWGFVDRILVGGILISIFAIVGVLFHLGSTTL
          EWG VDRILVGGILISIF IVG+LFHLGSTTL
Subjt:  -GEWGFVDRILVGGILISIFAIVGVLFHLGSTTL

SwissProt top hits

e value

%identity

Alignment

Q291H5 Threonylcarbamoyladenosine tRNA methylthiotransferase

6.9e-126

49.1

Show/hide

Query:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPK--IPGTQ-------------SDSEYMAGQLSAFGYLLSD
        ++DIEDL+         P        +V ++ KK+ ++K   DA++      PT  P + +  IPGTQ             SDSEYMAGQL+A+GY LS 
Subjt:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPK--IPGTQ-------------SDSEYMAGQLSAFGYLLSD

Query:  NPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH----------------------
          +EADLWL+N+CTVK+PS+      I    +  K +VVAGCVPQG+     L G+S++GVQQIDRVVEVVEETLKGH                      
Subjt:  NPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH----------------------

Query:  -EVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTN
         +VRKN  IEI+ IN GCL  CTYCKTKHARG L SY  + +V R R    EG  E+WL+SEDTGAYGRDIG +LP LL  +V  +P     MLR+GMTN
Subjt:  -EVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTN

Query:  PPFILEHLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQF
        PP+ILEHL+E+A VL HP VY+FLHVPVQSGSD++L  M REY   +F  VVD L E VPG+ IATDIICGFP ET+EDF +T+ L   Y+ P + I+QF
Subjt:  PPFILEHLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQF

Query:  YPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIE
        +PRPGTPAA+M+++P+ +VKKR++ LT +F ++ PY    G +  + +TEI+ D +H VGH K Y QVL+   + +LGT   V+ITSV ++S+ G++++
Subjt:  YPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIE

Q5VV42 Threonylcarbamoyladenosine tRNA methylthiotransferase

4.7e-122

52.04

Show/hide

Query:  SDSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH---
        SD EYMAGQL+A+GY +++N  +ADLWL+N+CTVK+P++      I K +   K +V+AGCVPQ       L+G+SI+GVQQIDRVVEVVEET+KGH   
Subjt:  SDSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH---

Query:  --------------------EVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNA
                            ++RKN  IEI+ IN GCL ACTYCKTKHARG+L SY +D LV R +    EGV E+WL+SEDTGAYGRDIG NLP+LL  
Subjt:  --------------------EVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNA

Query:  IVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFC
        +V  +P  A  MLR+GMTNPP+ILEHL+E+A +L HP VY+FLH+PVQS SD++L  M REY V +F+ VVD L E VPG+ IATDIICGFPGETD+DF 
Subjt:  IVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFC

Query:  QTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSA
        +T+ L++EYK P + I+QFYPRPGTPAA+M++VP+ + K+R+++L+ VF +++PY+   G  +++ +TE + D    V H + Y QVLV      +G   
Subjt:  QTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSA

Query:  MVKITSVGRWSVFGDVI
         V I   G+  + G  +
Subjt:  MVKITSVGRWSVFGDVI

Q6NS26 Threonylcarbamoyladenosine tRNA methylthiotransferase

3.2e-123

48.53

Show/hide

Query:  LTAVGIKPKKKNKLKSN--PDA-DDSSNQLSPTQNPLVPKIPGTQ-------------SDSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSAM
        ++A   KP  +   + N  P A   + N +   + P    IPGTQ             SD EYMAGQL+A+GY +++ PE+ADLWL+N+CTVKSP++   
Subjt:  LTAVGIKPKKKNKLKSN--PDA-DDSSNQLSPTQNPLVPKIPGTQ-------------SDSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSAM

Query:  DTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH-----------------------EVRKNKFIEILPINVGCLGACT
           I K + A K +VV+GCVPQ       ++G+SI+GVQQIDRVVEVVEET+KGH                       ++RKN  IEI+ IN GCL ACT
Subjt:  DTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH-----------------------EVRKNKFIEILPINVGCLGACT

Query:  YCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSF
        YCKTKHARG L SY V+ LV R      EGV E+WL+SEDTGAYGRDIG +LP+LL  +V  +P  A  MLR+GMTNPP+ILEHL+E+A +L HP VY+F
Subjt:  YCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSF

Query:  LHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRS
        LH+PVQS SD++L  M REY + +F+ VVD L E VPG+ IATDIICGFPGETDEDF +T+ L++EYK P + I+QFYPRPGTPAA+M++V + + K+R+
Subjt:  LHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRS

Query:  RELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIE
        +EL+ +F ++ PY+   G+ +++ +TE + D  + V H + Y QVLV      +G    VKI   G+  + G  ++
Subjt:  RELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIE

Q6P4Y0 Threonylcarbamoyladenosine tRNA methylthiotransferase

2.2e-124

48.53

Show/hide

Query:  LTAVGIKPKKKNKLKSN--PDA-DDSSNQLSPTQNPLVPKIPGTQ-------------SDSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSAM
        ++A   KP  +   + N  P A   + N++   + P    IPGTQ             SD EYMAGQL+A+GY +++ PE+ADLWL+N+CTVKSP++   
Subjt:  LTAVGIKPKKKNKLKSN--PDA-DDSSNQLSPTQNPLVPKIPGTQ-------------SDSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSAM

Query:  DTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH-----------------------EVRKNKFIEILPINVGCLGACT
           I K + A K +V++GCVPQ     + ++G+SI+GVQQIDRVVEVVEET+KGH                       ++RKN  IEI+ IN GCL ACT
Subjt:  DTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH-----------------------EVRKNKFIEILPINVGCLGACT

Query:  YCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSF
        YCKTKHARG L SY V+ LV R      EGV E+WL+SEDTGAYGRDIG +LP+LL  +V  +P  A  MLR+GMTNPP+ILEHL+E+A +L HP VY+F
Subjt:  YCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSF

Query:  LHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRS
        LH+PVQS SD++L  M REY + +F+ VVD L E VPG+ IATDIICGFPGETDEDF +T+ L++EYK P + I+QFYPRPGTPAA+M++VP+ + K+R+
Subjt:  LHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRS

Query:  RELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIE
        +EL+ +F +++PY+   G  + + +TE + D  + V H + Y QVLV      +G    VKI   G+  + G  ++
Subjt:  RELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIE

Q7K4W1 Threonylcarbamoyladenosine tRNA methylthiotransferase

3.4e-125

47.79

Show/hide

Query:  MEDIEDLLIGGGGGGGAPPGYRLP-LTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGTQ-------------SDSEYMAGQLSAFGYLLSDN
        ++DIEDL+          P  R      V ++ KK+++++   ++ +   +  PT +  V  IPGTQ             SDSEYMAGQL+A+GY LS  
Subjt:  MEDIEDLLIGGGGGGGAPPGYRLP-LTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGTQ-------------SDSEYMAGQLSAFGYLLSDN

Query:  PEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH-----------------------
         EEADLWL+N+CTVK+PS+      I       K +VVAGCVPQG+     L G+S++GVQQIDRVVEVVEETLKGH                       
Subjt:  PEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH-----------------------

Query:  -EVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTN
         +VRKN  IEI+ IN GCL  CTYCKTKHARG L SY  + +V+R R    EG  E+WL+SEDTGAYGRDIG +LP LL  +V  +P     MLR+GMTN
Subjt:  -EVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTN

Query:  PPFILEHLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQF
        PP+ILEHL+E+ANVL HP VYSFLHVPVQSGSD++L  M REY   +F  VVD L E VPG+ IATDIICGFP ET++DF +T+ L  +Y+ P + I+QF
Subjt:  PPFILEHLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQF

Query:  YPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVI--
        +PRPGTPAA+M ++P+ +VKKR++ LT +F ++ PY    G +  + +TE++ D +H VGH K Y QVL+   + +LGT   V+ITS  ++S+ G+++  
Subjt:  YPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVI--

Query:  ETISTRHHKTTTLEEMPIQDK
        E   TR  K   L  + +Q +
Subjt:  ETISTRHHKTTTLEEMPIQDK

Arabidopsis top hits

e value

%identity

Alignment

AT1G72090.1 Methylthiotransferase

2.2e-223

66.61

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Query:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPK--KKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSD
        MEDIEDLL   GG GGAPPG+RLPL AVGI PK  K  ++ S PD   +SN+ S    P   KIPGT             QSDSEYMAGQLSAFGY L++
Subjt:  MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPK--KKNKLKSNPDADDSSNQLSPTQNPLVPKIPGT-------------QSDSEYMAGQLSAFGYLLSD

Query:  NPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNK
         PEEADLWLINTCTVKSPSQSAM TLIT+ ++ KKPLV+AGCVPQGSRDLKELEGVS+VGVQQIDRVVE+VEETLKGHE                VR+N 
Subjt:  NPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE----------------VRKNK

Query:  FIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEH
        FIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV+RVRTVI+EGVKE+WLSSEDTGAYGRDIGVNLP LLNAIV ELP D STMLRIGMTNPPFILEH
Subjt:  FIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEH

Query:  LKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTP
        LKEIA VL HPCVY+FLHVPVQSGSD++L+AMNREYT  EFRTVVDTLTELVPGMQIATDIICGFPGETDEDF QT+ LIK+YK PQVHISQFYPRPGTP
Subjt:  LKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTP

Query:  AARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHK
        AA+MKKV S IVK+RSRELTSVFEAF PY GME R ERIWITE+A DGIHLVGHTKGY+QVLV  PE+MLGTSAM +ITSVGRWSVFG+VIET S+ + +
Subjt:  AARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHK

Query:  TTTLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASGEWG
        T + EE     K+PC +    C      E C C  ESC          + S NIS  ++ K K              +K+EE+E   +       +  WG
Subjt:  TTTLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASGEWG

Query:  FVDRILVGGILISIFAIVGVLFHLGSTTL
        F+D+ LV G+ +S   I+ +L  +    L
Subjt:  FVDRILVGGILISIFAIVGVLFHLGSTTL

AT4G36390.1 Methylthiotransferase

8.7e-23

23.32

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Query:  SDSEYMAGQLSAFGYL-LSDNPEEADLWLINTCTVKSPSQS----------------AMDTLITKCKNAKKP-LVVAGCVPQGSRDLKELEGVSIVGV--
        +D E +   +   GY  +  +PE A++  +NTC ++  ++                  ++    + K+ K P +VV GC+ +  +D K L+   +V V  
Subjt:  SDSEYMAGQLSAFGYL-LSDNPEEADLWLINTCTVKSPSQS----------------AMDTLITKCKNAKKP-LVVAGCVPQGSRDLKELEGVSIVGV--

Query:  -----QQIDRVVEVVEETLKGHEV---RKNKFIEILPINV-------------GCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSED
             + + R++E V+   KG       +  + +I P+ +             GC   C +C     RG   S  V+S+++ V  +   GVKEV L  ++
Subjt:  -----QQIDRVVEVVEETLKGHEV---RKNKFIEILPINV-------------GCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSED

Query:  TGAYGRD------------------------IGVNLPSLLNAIVAELPPDASTMLRIGMTNP-----PFILEHLKEIANVLCHPCVYSFLHVPVQSGSDA
          +Y  D                        +G+    LL+ +  E P      +R   T+P     P  L +L    + +C     + +H+P QSG+  
Subjt:  TGAYGRD------------------------IGVNLPSLLNAIVAELPPDASTMLRIGMTNP-----PFILEHLKEIANVLCHPCVYSFLHVPVQSGSDA

Query:  ILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAAR--MKKVPSAIVKKRSRELTSVFEA
        IL  M R YT + +  +V  +  ++P + I +D I GF GET+E+  +T++L++       ++  +  R  T A R     VP  + ++R  EL   F  
Subjt:  ILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAAR--MKKVPSAIVKKRSRELTSVFEA

Query:  FTP--YNGMEGRVERIWI--TEIAADGIHLVGHT-KGYIQVLVIAP
         T   Y+   G  + + +      A    L+G T KG+    V  P
Subjt:  FTP--YNGMEGRVERIWI--TEIAADGIHLVGHT-KGYIQVLVIAP

Sequences

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CDS sequence

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ATGGAAGACATAGAAGACCTGCTGATCGGCGGCGGTGGAGGAGGCGGCGCTCCCCCTGGTTACCGGCTTCCGTTAACCGCAGTGGGTATAAAGCCGAAGAAGAAGAATAA
GCTCAAATCCAATCCTGATGCCGATGATTCCAGCAACCAGCTCTCTCCGACCCAAAATCCACTTGTTCCTAAGATTCCAGGCACCCAGAGCGACAGTGAATACATGGCTG
GTCAGCTTTCAGCATTTGGTTATTTGTTAAGTGACAATCCTGAAGAGGCAGACTTGTGGCTTATAAATACTTGCACAGTTAAATCCCCTAGCCAGTCTGCTATGGACACT
CTAATAACAAAATGCAAGAATGCGAAAAAGCCTCTTGTGGTAGCTGGGTGTGTACCACAAGGTAGTAGAGATTTAAAGGAGCTAGAGGGGGTCAGTATAGTAGGAGTCCA
GCAGATTGACCGTGTGGTAGAAGTTGTGGAAGAGACACTGAAAGGTCATGAAGTAAGGAAGAACAAGTTCATTGAAATTCTTCCAATCAATGTTGGTTGTTTAGGTGCTT
GCACTTATTGTAAGACAAAACATGCTCGTGGTCACTTGGGAAGTTACACTGTAGATAGCCTTGTGAAGCGTGTAAGAACTGTCATCAACGAAGGAGTCAAGGAAGTATGG
TTGAGCAGTGAAGATACTGGAGCGTATGGCCGTGACATTGGGGTCAATCTTCCAAGTTTATTAAATGCAATTGTTGCTGAGCTTCCACCGGATGCAAGCACAATGCTTCG
GATTGGGATGACAAATCCTCCTTTTATTCTTGAGCACTTGAAAGAGATAGCTAATGTCTTGTGTCATCCATGCGTATACTCCTTTTTGCATGTGCCAGTTCAATCTGGAA
GTGACGCAATCTTGAGTGCAATGAATCGAGAATATACAGTCGATGAATTCAGGACTGTAGTAGATACCTTAACTGAGCTTGTTCCTGGGATGCAGATTGCAACTGATATA
ATATGTGGATTTCCTGGGGAAACGGATGAAGATTTTTGTCAAACTATCAACCTTATTAAGGAGTACAAATTACCTCAAGTTCATATCTCACAGTTCTATCCTCGACCAGG
GACACCTGCTGCTAGGATGAAGAAAGTTCCAAGTGCTATTGTCAAGAAACGAAGCCGTGAGTTGACTTCTGTTTTCGAAGCTTTTACACCATATAATGGAATGGAGGGCA
GAGTGGAAAGAATTTGGATAACAGAAATTGCAGCAGATGGCATTCACTTGGTTGGCCACACCAAGGGATACATACAGGTGCTCGTAATTGCCCCAGAAACCATGCTGGGA
ACTTCAGCCATGGTGAAGATAACATCCGTTGGAAGGTGGTCCGTTTTTGGGGACGTGATCGAAACCATCAGCACCAGACATCATAAAACAACTACTCTCGAAGAGATGCC
GATCCAAGACAAAGTTCCTTGCTGTAACACCCACGAGAAGTGTTCAAGTTCCACAGAACCCGAACCCTGTGCTTGTGGGCCAGAAAGCTGCAAAGCACCAGTAACTCTGG
ATAATAAAGATGATTCTTCAAGTAATATTTCATTGGCTGAAGAGCCAAAACGGAAAAATGTGATAGAATGGCTACTGAGGAGACGGAAGAGGCATGTGGCCAAGAGAGAG
GAGAGAGAAAGTAGTGGCATAGCATCTGAGAGAAAGCAGGCATCGGGTGAGTGGGGCTTTGTTGATAGGATTCTTGTTGGTGGAATACTAATCAGTATCTTTGCAATTGT
TGGTGTACTGTTTCATCTTGGATCCACCACTCTT

mRNA sequence

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Protein sequence

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MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGTQSDSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSAMDT
LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVW
LSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDI
ICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLG
TSAMVKITSVGRWSVFGDVIETISTRHHKTTTLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKRE
ERESSGIASERKQASGEWGFVDRILVGGILISIFAIVGVLFHLGSTTL

MS015457 (gene) of Bitter gourd (TR) v1 genome