CuGenDBv2

Gene ID	MS015514
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	3-isopropylmalate dehydratase
Genome location	scaffold300:57254..57373
RNA-Seq Expression	MS015514
Synteny	MS015514
Gene Ontology terms	GO:0009098 - leucine biosynthetic process (biological process) GO:0019761 - glucosinolate biosynthetic process (biological process) GO:0003861 - 3-isopropylmalate dehydratase activity (molecular function) GO:0046872 - metal ion binding (molecular function) GO:0050486 - intramolecular transferase activity, transferring hydroxy groups (molecular function) GO:0051539 - 4 iron, 4 sulfur cluster binding (molecular function)
InterPro domains	IPR015931 - Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3 IPR036008 - Aconitase, iron-sulfur domain

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6595175.1 3-isopropylmalate dehydratase large subunit, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]

5.8e-10

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        VLLGTDSHTC A AFGQFASGIGNT+AGFVLGT KSLLKV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

XP_008465910.1 PREDICTED: 3-isopropylmalate dehydratase large subunit [Cucumis melo]

5.8e-10

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        VLLGTDSHTC A AFGQFASGIGNT+AGFVLGT KSLLKV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

XP_022132847.1 3-isopropylmalate dehydratase large subunit, chloroplastic [Momordica charantia]

5.8e-10

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        VLLGTDSHTC A AFGQFASGIGNT+AGFVLGT KSLLKV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

XP_022963044.1 3-isopropylmalate dehydratase large subunit, chloroplastic [Cucurbita moschata]

5.8e-10

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        VLLGTDSHTC A AFGQFASGIGNT+AGFVLGT KSLLKV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

XP_022972644.1 3-isopropylmalate dehydratase large subunit, chloroplastic [Cucurbita maxima]

5.8e-10

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        VLLGTDSHTC A AFGQFASGIGNT+AGFVLGT KSLLKV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

TrEMBL top hits

e value

%identity

Alignment

A0A1S3CQ00 3-isopropylmalate dehydratase

2.8e-10

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        VLLGTDSHTC A AFGQFASGIGNT+AGFVLGT KSLLKV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

A0A5D3E4R8 3-isopropylmalate dehydratase

2.8e-10

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        VLLGTDSHTC A AFGQFASGIGNT+AGFVLGT KSLLKV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

A0A6J1BUZ5 3-isopropylmalate dehydratase

2.8e-10

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        VLLGTDSHTC A AFGQFASGIGNT+AGFVLGT KSLLKV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

A0A6J1HIW0 3-isopropylmalate dehydratase

2.8e-10

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        VLLGTDSHTC A AFGQFASGIGNT+AGFVLGT KSLLKV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

A0A6J1IC64 3-isopropylmalate dehydratase

2.8e-10

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        VLLGTDSHTC A AFGQFASGIGNT+AGFVLGT KSLLKV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

SwissProt top hits

e value

%identity

Alignment

O27439 Probable 3-isopropylmalate dehydratase large subunit

8.5e-04

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        V++GTDSHTC   A G FA+GIG+T+   V  T K   +V
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

O28316 3-isopropylmalate dehydratase large subunit 1

1.0e-04

52.5

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        +++G DSHTC+  A G FA+GIG+T+ GFVL   K   KV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

P81291 Isopropylmalate/citramalate isomerase large subunit

3.8e-04

52.5

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        V++G DSHTC   AFG FA+GIG+T+   V  T K   KV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

Q94AR8 3-isopropylmalate dehydratase large subunit, chloroplastic

8.5e-12

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        VLLGTDSHTC A AFGQFA+GIGNT+AGFVLGT K LLKV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

Arabidopsis top hits

e value

%identity

Alignment

AT4G13430.1 isopropyl malate isomerase large subunit 1

6.0e-13

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Query:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV
        VLLGTDSHTC A AFGQFA+GIGNT+AGFVLGT K LLKV
Subjt:  VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

Sequences

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CDS sequence

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GTTCTGCTTGGTACAGACTCTCATACCTGTATAGCTAGTGCATTTGGTCAATTTGCTAGTGGAATTGGTAACACTAATGCAGGATTTGTACTAGGCACTCGGAAATCACT
GCTCAAGGTC

mRNA sequence

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GTTCTGCTTGGTACAGACTCTCATACCTGTATAGCTAGTGCATTTGGTCAATTTGCTAGTGGAATTGGTAACACTAATGCAGGATTTGTACTAGGCACTCGGAAATCACT
GCTCAAGGTC

Protein sequence

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VLLGTDSHTCIASAFGQFASGIGNTNAGFVLGTRKSLLKV

MS015514 (gene) of Bitter gourd (TR) v1 genome