CuGenDBv2

Gene ID	MS016228
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	phosphatidyl-N-methylethanolamine N-methyltransferase
Genome location	scaffold9_2:1242869..1245476
RNA-Seq Expression	MS016228
Synteny	MS016228
Gene Ontology terms	GO:0006656 - phosphatidylcholine biosynthetic process (biological process) GO:0032259 - methylation (biological process) GO:0005789 - endoplasmic reticulum membrane (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0000773 - phosphatidyl-N-methylethanolamine N-methyltransferase activity (molecular function) GO:0004608 - phosphatidylethanolamine N-methyltransferase activity (molecular function) GO:0080101 - phosphatidyl-N-dimethylethanolamine N-methyltransferase activity (molecular function)
InterPro domains	IPR007318 - Phospholipid methyltransferase IPR024960 - Phosphatidyl-N-methylethanolamine/Phosphatidylethanolamine N-methyltransferase

Homology

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GenBank top hits

e value

%identity

Alignment

KAG7036543.1 Phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma]

3.2e-65

88.55

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Query:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        M ++A IGVLLPFP+YYVLWTRPQLW N CGKW EP KLMA+VS+FLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGK
Subjt:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
        NIPWVTEFPFGYI+DPQYVGSILS+ ACLSW
Subjt:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW

XP_004137657.1 phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis sativus]

1.3e-66

90.84

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Query:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MGI A IGVLLPFP+YYVLW RPQLW N CGK REPCKLMA+VS+FLKLLQFLSI+HVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
Subjt:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
        NIPWVTEFPFGYI+DPQYVGSILS+FACLSW
Subjt:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW

XP_008465151.2 PREDICTED: phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis melo]

7.6e-67

90.84

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Query:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MGI+A IGVLLPFP+YYVLW RPQLW N CGK REPCKLMA+VS+FLKLLQFLSI+HVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
Subjt:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
        NIPWVTEFPFGYI+DPQYVGSILS+FACLSW
Subjt:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW

XP_022153398.1 phosphatidyl-N-methylethanolamine N-methyltransferase [Momordica charantia]

3.2e-73

100

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Query:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
Subjt:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
        NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
Subjt:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW

XP_038903463.1 phosphatidyl-N-methylethanolamine N-methyltransferase [Benincasa hispida]

1.9e-65

87.79

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Query:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MGI+A +GVLLPFP+YYVLW+RPQLW N CGK R+PCKLMA+VS+FLKLLQFLSI HVSSF+WPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGK
Subjt:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
        NIPWVTEFPFGYI+DPQYVGSILS+FACLSW
Subjt:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LCJ2 Uncharacterized protein

6.3e-67

90.84

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Query:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MGI A IGVLLPFP+YYVLW RPQLW N CGK REPCKLMA+VS+FLKLLQFLSI+HVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
Subjt:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
        NIPWVTEFPFGYI+DPQYVGSILS+FACLSW
Subjt:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW

A0A1S3CPQ5 phosphatidyl-N-methylethanolamine N-methyltransferase

3.7e-67

90.84

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Query:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MGI+A IGVLLPFP+YYVLW RPQLW N CGK REPCKLMA+VS+FLKLLQFLSI+HVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
Subjt:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
        NIPWVTEFPFGYI+DPQYVGSILS+FACLSW
Subjt:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW

A0A6J1DHC8 phosphatidyl-N-methylethanolamine N-methyltransferase

1.5e-73

100

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Query:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
Subjt:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
        NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
Subjt:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW

A0A6J1GCG7 phosphatidyl-N-methylethanolamine N-methyltransferase

7.7e-65

87.79

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Query:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        M ++A IGVLLPFP+YYVLWTRPQLW N CGKW EP KLMA+VS+FLKLLQ LSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGK
Subjt:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
        NIPWVTEFPFGYI+DPQYVGSILS+ ACLSW
Subjt:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW

A0A6J1KB80 phosphatidyl-N-methylethanolamine N-methyltransferase

3.5e-65

87.79

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Query:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        M ++A IGVLLPFP+YY+LWTRPQLW N CGKW EP KLMA+VS+FLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGK
Subjt:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
        NIPWVTEFPFGYI+DPQYVGSILS+ ACLSW
Subjt:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW

SwissProt top hits

e value

%identity

Alignment

Q9SAH5 Phosphatidyl-N-methylethanolamine N-methyltransferase

1.7e-56

75.57

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Query:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MG++A IGVLLPFPFY+ LWT  Q W N CG+ R+P  +MARVS  LK  Q LS+  V+S SWPPPLYFWPLMAFGQFLNFRVYQLLGE+G YYGVRFGK
Subjt:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
        NIPWVTEFPFG IRDPQYVGSI+S+ ACLSW
Subjt:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW

Arabidopsis top hits

e value

%identity

Alignment

AT1G80860.1 phospholipid N-methyltransferase

1.2e-57

75.57

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Query:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MG++A IGVLLPFPFY+ LWT  Q W N CG+ R+P  +MARVS  LK  Q LS+  V+S SWPPPLYFWPLMAFGQFLNFRVYQLLGE+G YYGVRFGK
Subjt:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
        NIPWVTEFPFG IRDPQYVGSI+S+ ACLSW
Subjt:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW

AT1G80860.2 phospholipid N-methyltransferase

1.2e-57

75.57

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Query:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MG++A IGVLLPFPFY+ LWT  Q W N CG+ R+P  +MARVS  LK  Q LS+  V+S SWPPPLYFWPLMAFGQFLNFRVYQLLGE+G YYGVRFGK
Subjt:  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW
        NIPWVTEFPFG IRDPQYVGSI+S+ ACLSW
Subjt:  NIPWVTEFPFGYIRDPQYVGSILSIFACLSW

Sequences

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CDS sequence

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ATGGGCATAATCGCAGGAATTGGAGTGCTGCTGCCATTTCCCTTCTACTACGTCTTATGGACTCGTCCACAGTTATGGGCGAATTTTTGCGGGAAATGGCGCGAACCCTG
CAAATTGATGGCTCGGGTCTCCATTTTTCTCAAGTTGCTTCAGTTCCTCTCCATCCTCCACGTCTCCTCCTTCTCATGGCCGCCGCCTCTCTACTTCTGGCCCCTCATGG
CCTTTGGCCAATTTTTGAACTTCAGGGTCTATCAGTTACTGGGTGAATCCGGTGTTTACTATGGAGTACGGTTTGGGAAGAACATTCCATGGGTGACAGAATTTCCATTT
GGATATATAAGAGATCCTCAGTATGTGGGAAGCATTTTGAGCATTTTTGCTTGTCTTTCTTGG

mRNA sequence

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Protein sequence

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MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPF
GYIRDPQYVGSILSIFACLSW

MS016228 (gene) of Bitter gourd (TR) v1 genome