CuGenDBv2

Gene ID	MS019395
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	tRNA_int_end_N2 domain-containing protein
Genome location	scaffold28:41008..41378
RNA-Seq Expression	MS019395
Synteny	MS019395
Gene Ontology terms	GO:0000379 - tRNA-type intron splice site recognition and cleavage (biological process) GO:0090305 - nucleic acid phosphodiester bond hydrolysis (biological process) GO:0000214 - tRNA-intron endonuclease complex (cellular component) GO:0004519 - endonuclease activity (molecular function)
InterPro domains	IPR024337 - tRNA-splicing endonuclease, subunit Sen54

Homology

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GenBank top hits

e value

%identity

Alignment

XP_022156818.1 uncharacterized protein LOC111023660 [Momordica charantia]

1.6e-50

86.82

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Query:  EDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISELL
        E+TLFLMEVGALHLLDHDNSSLSLKD YKKVAEGK GCLWEQFEVYRHLKSLGFIVGK KVPWSVK V NGSDISP+SSIEN+GAWDLES+DE SISELL
Subjt:  EDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISELL

Query:  SSIQL-----ILYVFLPHSKFRKSSPGDP
        SSIQL     I  VFLPHSKFRKSSPGDP
Subjt:  SSIQL-----ILYVFLPHSKFRKSSPGDP

XP_022973337.1 tRNA-splicing endonuclease subunit Sen54 isoform X1 [Cucurbita maxima]

6.5e-44

76.92

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Query:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL
        IE+TLFL+EVGALHLLDHDNSSLSLKD YKKVAEGK  C+WEQFEVYRHLKSLG+IVGK KVPWSVK   NG DIS RSSIENKG+ D ES DE SI EL
Subjt:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL

Query:  LSSIQL-----ILYVFLPHSKFRKSSPGDP
        L+++QL     I  V+LPHSKFRKSSPGDP
Subjt:  LSSIQL-----ILYVFLPHSKFRKSSPGDP

XP_022973338.1 tRNA-splicing endonuclease subunit Sen54 isoform X2 [Cucurbita maxima]

6.5e-44

76.92

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Query:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL
        IE+TLFL+EVGALHLLDHDNSSLSLKD YKKVAEGK  C+WEQFEVYRHLKSLG+IVGK KVPWSVK   NG DIS RSSIENKG+ D ES DE SI EL
Subjt:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL

Query:  LSSIQL-----ILYVFLPHSKFRKSSPGDP
        L+++QL     I  V+LPHSKFRKSSPGDP
Subjt:  LSSIQL-----ILYVFLPHSKFRKSSPGDP

XP_022973340.1 tRNA-splicing endonuclease subunit Sen54 isoform X3 [Cucurbita maxima]

6.5e-44

76.92

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Query:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL
        IE+TLFL+EVGALHLLDHDNSSLSLKD YKKVAEGK  C+WEQFEVYRHLKSLG+IVGK KVPWSVK   NG DIS RSSIENKG+ D ES DE SI EL
Subjt:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL

Query:  LSSIQL-----ILYVFLPHSKFRKSSPGDP
        L+++QL     I  V+LPHSKFRKSSPGDP
Subjt:  LSSIQL-----ILYVFLPHSKFRKSSPGDP

XP_038883355.1 uncharacterized protein LOC120074337 [Benincasa hispida]

1.1e-43

77.69

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Query:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL
        IE+TLFL+EVGALHLLDHDNSSLSL+D YKK+AEGK GCLWEQFEVYRHLKSLGFIVGK KVPWS+K V +GS+IS  SSIENKGA D++S DE SISEL
Subjt:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL

Query:  LSSIQL-----ILYVFLPHSKFRKSSPGDP
        L  IQL     I  VFLPH+KFRKSSPGDP
Subjt:  LSSIQL-----ILYVFLPHSKFRKSSPGDP

TrEMBL top hits

e value

%identity

Alignment

A0A6J1DRN3 uncharacterized protein LOC111023660

7.7e-51

86.82

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Query:  EDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISELL
        E+TLFLMEVGALHLLDHDNSSLSLKD YKKVAEGK GCLWEQFEVYRHLKSLGFIVGK KVPWSVK V NGSDISP+SSIEN+GAWDLES+DE SISELL
Subjt:  EDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISELL

Query:  SSIQL-----ILYVFLPHSKFRKSSPGDP
        SSIQL     I  VFLPHSKFRKSSPGDP
Subjt:  SSIQL-----ILYVFLPHSKFRKSSPGDP

A0A6J1FKK4 tRNA-splicing endonuclease subunit Sen54

7.7e-43

75.38

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Query:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL
        IE+TLFL+EVGALHLLDHDNSSLSLKD YKKVAEGK  C+WEQFEVYRHLKSLG+IVGK KVPWSVK   NG DIS +SSIENKG+ D  S DE SI EL
Subjt:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL

Query:  LSSIQL-----ILYVFLPHSKFRKSSPGDP
        + +IQL     I  V+LPHSKFRKSSPGDP
Subjt:  LSSIQL-----ILYVFLPHSKFRKSSPGDP

A0A6J1I794 tRNA-splicing endonuclease subunit Sen54 isoform X1

3.1e-44

76.92

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Query:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL
        IE+TLFL+EVGALHLLDHDNSSLSLKD YKKVAEGK  C+WEQFEVYRHLKSLG+IVGK KVPWSVK   NG DIS RSSIENKG+ D ES DE SI EL
Subjt:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL

Query:  LSSIQL-----ILYVFLPHSKFRKSSPGDP
        L+++QL     I  V+LPHSKFRKSSPGDP
Subjt:  LSSIQL-----ILYVFLPHSKFRKSSPGDP

A0A6J1I8D9 tRNA-splicing endonuclease subunit Sen54 isoform X3

3.1e-44

76.92

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Query:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL
        IE+TLFL+EVGALHLLDHDNSSLSLKD YKKVAEGK  C+WEQFEVYRHLKSLG+IVGK KVPWSVK   NG DIS RSSIENKG+ D ES DE SI EL
Subjt:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL

Query:  LSSIQL-----ILYVFLPHSKFRKSSPGDP
        L+++QL     I  V+LPHSKFRKSSPGDP
Subjt:  LSSIQL-----ILYVFLPHSKFRKSSPGDP

A0A6J1ICR8 tRNA-splicing endonuclease subunit Sen54 isoform X2

3.1e-44

76.92

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Query:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL
        IE+TLFL+EVGALHLLDHDNSSLSLKD YKKVAEGK  C+WEQFEVYRHLKSLG+IVGK KVPWSVK   NG DIS RSSIENKG+ D ES DE SI EL
Subjt:  IEDTLFLMEVGALHLLDHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVK-VSNGSDISPRSSIENKGAWDLESRDE-SISEL

Query:  LSSIQL-----ILYVFLPHSKFRKSSPGDP
        L+++QL     I  V+LPHSKFRKSSPGDP
Subjt:  LSSIQL-----ILYVFLPHSKFRKSSPGDP

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT3G02370.1 unknown protein

7.9e-24

43.41

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Query:  IEDTLFLMEVGALHLL-DHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVKVSNGSDISPRSSIENKGAWDLESRDESISELL
        IE+ L+L E+G L LL D D+  +SLKD Y ++AEGK+GC WE +EVYR+LK LG+I+G+  VPW+ K +   + +P    E+  A +     +S+++LL
Subjt:  IEDTLFLMEVGALHLL-DHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVKVSNGSDISPRSSIENKGAWDLESRDESISELL

Query:  SSIQL-----ILYVFLPHSKFRKSSPGDP
        S + +     +  V+LP+S+F+KSSPG+P
Subjt:  SSIQL-----ILYVFLPHSKFRKSSPGDP

AT3G02370.2 unknown protein

7.9e-24

43.41

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Query:  IEDTLFLMEVGALHLL-DHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVKVSNGSDISPRSSIENKGAWDLESRDESISELL
        IE+ L+L E+G L LL D D+  +SLKD Y ++AEGK+GC WE +EVYR+LK LG+I+G+  VPW+ K +   + +P    E+  A +     +S+++LL
Subjt:  IEDTLFLMEVGALHLL-DHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVKVSNGSDISPRSSIENKGAWDLESRDESISELL

Query:  SSIQL-----ILYVFLPHSKFRKSSPGDP
        S + +     +  V+LP+S+F+KSSPG+P
Subjt:  SSIQL-----ILYVFLPHSKFRKSSPGDP

AT3G57360.1 unknown protein

4.8e-21

39.53

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Query:  IEDTLFLMEVGALHLL-DHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVKVSNGSDISPRSSIENKGAWDLESRDESISELL
        IE+ L+L E+G L +L + D+  + LKD Y+K+AE K GC WE +EVYR+LK LG+I+G+  V W++K        P    E+  A +  + ++++++LL
Subjt:  IEDTLFLMEVGALHLL-DHDNSSLSLKD-YKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVKVSNGSDISPRSSIENKGAWDLESRDESISELL

Query:  SSIQL-----ILYVFLPHSKFRKSSPGDP
          +Q+     +  V+LP+S+F+KSSPG+P
Subjt:  SSIQL-----ILYVFLPHSKFRKSSPGDP

Sequences

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CDS sequence

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ATCGAGGACACTTTATTTCTTATGGAAGTTGGGGCCTTGCATCTTCTGGATCATGATAATTCAAGTCTTTCTTTGAAAGATTATAAGAAGGTAGCTGAAGGAAAATTCGG
ATGTCTCTGGGAGCAATTTGAGGTTTATAGGCACCTCAAGTCTCTTGGTTTCATTGTTGGAAAGCCTAAAGTTCCTTGGTCTGTGAAGGTTAGTAATGGCAGTGACATTT
CTCCTCGAAGTTCTATTGAGAACAAAGGAGCATGGGATTTGGAATCAAGAGATGAGAGTATCTCTGAACTATTAAGTTCCATTCAGCTCATTTTATATGTTTTTCTTCCA
CACAGCAAGTTTAGAAAGTCTTCTCCTGGTGACCCT

mRNA sequence

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Protein sequence

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IEDTLFLMEVGALHLLDHDNSSLSLKDYKKVAEGKFGCLWEQFEVYRHLKSLGFIVGKPKVPWSVKVSNGSDISPRSSIENKGAWDLESRDESISELLSSIQLILYVFLP
HSKFRKSSPGDP

MS019395 (gene) of Bitter gourd (TR) v1 genome