CuGenDBv2

Gene ID	MS020525
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	mediator of RNA polymerase II transcription subunit 32
Genome location	scaffold375:890480..890920
RNA-Seq Expression	MS020525
Synteny	MS020525
Gene Ontology terms	GO:0006355 - regulation of transcription, DNA-templated (biological process) GO:0009631 - cold acclimation (biological process) GO:0010150 - leaf senescence (biological process) GO:0048364 - root development (biological process) GO:0016592 - mediator complex (cellular component)
InterPro domains	IPR033244 - Mediator of RNA polymerase II transcription subunit 32

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0045925.1 mediator of RNA polymerase II transcription subunit 32 [Cucumis melo var. makuwa]

1.0e-64

93.88

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA
        MDSLVDSLNNAYQEFVAGAANVLEANE+SGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSL GKSGQ +  TGLPPISA
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA

Query:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ
        VRLEQMSKAVRWLVIELQ GSGAAASASAHSH SAPFDARFTEDA Q
Subjt:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ

XP_004148855.1 mediator of RNA polymerase II transcription subunit 32 [Cucumis sativus]

1.8e-64

93.88

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA
        MDSLVDSLNNAYQEFVAGAANVLEANE+SGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSL GKSGQ +A T LPPISA
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA

Query:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ
        VRLEQMSKAVRWLVIELQ GSGAAASASAHSH SAPFDARFTEDA Q
Subjt:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ

XP_022148505.1 mediator of RNA polymerase II transcription subunit 32 [Momordica charantia]

5.7e-71

100

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA
        MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA

Query:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ
        VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ
Subjt:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ

XP_022986622.1 mediator of RNA polymerase II transcription subunit 32-like isoform X2 [Cucurbita maxima]

2.3e-64

93.29

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAA-ATGLPPIS
        MDSLVDSLNNAYQEFV+GAANVLEANE+SGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSL GKSGQ AA  TGLPPIS
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAA-ATGLPPIS

Query:  AVRLEQMSKAVRWLVIELQQGSG-AAASASAHSHPSAPFDARFTEDAAQ
        A+RLEQMSKAVRWLVIELQ GSG AAASASAHSHPSAPFDARFTEDA Q
Subjt:  AVRLEQMSKAVRWLVIELQQGSG-AAASASAHSHPSAPFDARFTEDAAQ

XP_038902813.1 mediator of RNA polymerase II transcription subunit 32 [Benincasa hispida]

2.8e-65

94.56

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA
        MDSLVDSLNNAYQEFVAGAANVLEANE+SGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSL GKSGQ AA TG+PPISA
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA

Query:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ
        VRLEQMSKAVRWLVIELQ GSGAAASASAHSH SAPFDARFTEDA Q
Subjt:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LIN7 Uncharacterized protein

8.7e-65

93.88

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA
        MDSLVDSLNNAYQEFVAGAANVLEANE+SGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSL GKSGQ +A T LPPISA
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA

Query:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ
        VRLEQMSKAVRWLVIELQ GSGAAASASAHSH SAPFDARFTEDA Q
Subjt:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ

A0A5A7TWZ7 Mediator of RNA polymerase II transcription subunit 32

5.1e-65

93.88

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA
        MDSLVDSLNNAYQEFVAGAANVLEANE+SGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSL GKSGQ +  TGLPPISA
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA

Query:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ
        VRLEQMSKAVRWLVIELQ GSGAAASASAHSH SAPFDARFTEDA Q
Subjt:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ

A0A6J1D330 mediator of RNA polymerase II transcription subunit 32

2.8e-71

100

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA
        MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISA

Query:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ
        VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ
Subjt:  VRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ

A0A6J1JEJ1 mediator of RNA polymerase II transcription subunit 32-like isoform X1

1.1e-64

93.29

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAA-ATGLPPIS
        MDSLVDSLNNAYQEFV+GAANVLEANE+SGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSL GKSGQ AA  TGLPPIS
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAA-ATGLPPIS

Query:  AVRLEQMSKAVRWLVIELQQGSG-AAASASAHSHPSAPFDARFTEDAAQ
        A+RLEQMSKAVRWLVIELQ GSG AAASASAHSHPSAPFDARFTEDA Q
Subjt:  AVRLEQMSKAVRWLVIELQQGSG-AAASASAHSHPSAPFDARFTEDAAQ

A0A6J1JH28 mediator of RNA polymerase II transcription subunit 32-like isoform X2

1.1e-64

93.29

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAA-ATGLPPIS
        MDSLVDSLNNAYQEFV+GAANVLEANE+SGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSL GKSGQ AA  TGLPPIS
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAA-ATGLPPIS

Query:  AVRLEQMSKAVRWLVIELQQGSG-AAASASAHSHPSAPFDARFTEDAAQ
        A+RLEQMSKAVRWLVIELQ GSG AAASASAHSHPSAPFDARFTEDA Q
Subjt:  AVRLEQMSKAVRWLVIELQQGSG-AAASASAHSHPSAPFDARFTEDAAQ

SwissProt top hits

e value

%identity

Alignment

Q84VW5 Mediator of RNA polymerase II transcription subunit 32

3.0e-46

68.63

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPA------AATG
        MD++VDSLN AY++FV  +A VLE+ ES+G QK   TD ALENFK+KWELFRVACDQAEEFVESVKQRIGSECLVDEATG  T  +G  A      AAT 
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPA------AATG

Query:  LPPISAVRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ
        LPPISAVRLEQMS+AVRWLV+ELQ+GSG  A  S HS  S  FD+RF+ED+ Q
Subjt:  LPPISAVRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ

Arabidopsis top hits

e value

%identity

Alignment

AT1G11760.1 MED32; Has 41 Blast hits to 40 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).

2.1e-47

68.63

Show/hide

Query:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPA------AATG
        MD++VDSLN AY++FV  +A VLE+ ES+G QK   TD ALENFK+KWELFRVACDQAEEFVESVKQRIGSECLVDEATG  T  +G  A      AAT 
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPA------AATG

Query:  LPPISAVRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ
        LPPISAVRLEQMS+AVRWLV+ELQ+GSG  A  S HS  S  FD+RF+ED+ Q
Subjt:  LPPISAVRLEQMSKAVRWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ

Sequences

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CDS sequence

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ATGGACAGCTTAGTGGACTCTTTAAATAACGCGTACCAGGAGTTTGTTGCTGGGGCAGCCAATGTTCTTGAAGCCAACGAAAGCTCTGGGGCTCAGAAGACAGCAGCAAC
AGATGCCGCTCTTGAGAATTTTAAACAGAAATGGGAGCTATTCAGGGTTGCCTGTGACCAAGCAGAGGAGTTTGTGGAGTCTGTGAAGCAGAGGATAGGATCAGAGTGCC
TGGTGGATGAGGCCACTGGTTCACTGACTGGCAAGTCTGGGCAGCCTGCCGCTGCCACCGGTCTTCCACCAATCAGTGCCGTTCGGCTGGAACAGATGAGCAAAGCTGTG
AGATGGCTTGTGATTGAACTGCAGCAGGGCTCAGGAGCTGCTGCTTCTGCTTCAGCACATTCTCACCCTTCTGCCCCTTTTGATGCTAGATTTACCGAGGATGCAGCCCA
A

mRNA sequence

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Protein sequence

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MDSLVDSLNNAYQEFVAGAANVLEANESSGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLTGKSGQPAAATGLPPISAVRLEQMSKAV
RWLVIELQQGSGAAASASAHSHPSAPFDARFTEDAAQ

MS020525 (gene) of Bitter gourd (TR) v1 genome