CuGenDBv2

Gene ID	Moc08g40070
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	(R)-mandelonitrile lyase 1-like
Genome location	chr8:30714608..30715387
RNA-Seq Expression	Moc08g40070
Synteny	Moc08g40070
Gene Ontology terms	GO:0016614 - oxidoreductase activity, acting on CH-OH group of donors (molecular function) GO:0016829 - lyase activity (molecular function) GO:0050660 - flavin adenine dinucleotide binding (molecular function)
InterPro domains	IPR000172 - Glucose-methanol-choline oxidoreductase, N-terminal IPR036188 - FAD/NAD(P)-binding domain superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

XP_022153313.1 (R)-mandelonitrile lyase 1-like [Momordica charantia]

1.1e-18

91.38

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Query:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        GAVE LNKANPANLKVVI ATVERIIFSGLSA+GVSYSDSEGKLHTAFIR KGEI LS
Subjt:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS

XP_022153314.1 (R)-mandelonitrile lyase 1-like [Momordica charantia]

6.2e-19

91.38

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Query:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        GAVE LNKANP NLKV I ATVERIIFSGLSA+GVSYSDSEGKLHTAFIRNKGEIILS
Subjt:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS

XP_022153315.1 (R)-mandelonitrile lyase 1-like [Momordica charantia]

3.1e-18

87.93

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Query:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        GAVE LNKANP N+KV I  TVERIIFSGLSA+GVSYSDSEGKLHTAFIRNKGEIILS
Subjt:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS

XP_023538274.1 (R)-mandelonitrile lyase 1-like [Cucurbita pepo subsp. pepo]

8.4e-16

81.03

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Query:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        GAVEFLNK    NLKVV+ A+V RIIFSGLSATGVSYSDS+GK HTA+IRNKGEIILS
Subjt:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS

XP_038889318.1 (R)-mandelonitrile lyase 3-like [Benincasa hispida]

1.4e-15

79.31

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Query:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        GAVE LNKA P NLKV + ATV+RI+FSGLSA GV YSDS+GKLHTAFIR KGEIILS
Subjt:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS

TrEMBL top hits

e value

%identity

Alignment

A0A5A7VGR6 (R)-mandelonitrile lyase 1-like

1.2e-15

79.31

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Query:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        GAVEFLNKAN  N+KV I ATV+RI+FSGLSA GV YSDS+GKLH AFIR KGEIILS
Subjt:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS

A0A5D3CKE4 (R)-mandelonitrile lyase 1-like

1.2e-15

79.31

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Query:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        GAVEFLNKAN  N+KV I ATV+RI+FSGLSA GV YSDS+GKLH AFIR KGEIILS
Subjt:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS

A0A6J1DGG8 (R)-mandelonitrile lyase 1-like

1.5e-18

87.93

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Query:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        GAVE LNKANP N+KV I  TVERIIFSGLSA+GVSYSDSEGKLHTAFIRNKGEIILS
Subjt:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS

A0A6J1DH55 (R)-mandelonitrile lyase 1-like

5.2e-19

91.38

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Query:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        GAVE LNKANPANLKVVI ATVERIIFSGLSA+GVSYSDSEGKLHTAFIR KGEI LS
Subjt:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS

A0A6J1DIQ0 (R)-mandelonitrile lyase 1-like

3.0e-19

91.38

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Query:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        GAVE LNKANP NLKV I ATVERIIFSGLSA+GVSYSDSEGKLHTAFIRNKGEIILS
Subjt:  GAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILS

SwissProt top hits

e value

%identity

Alignment

O50048 (R)-mandelonitrile lyase 2

1.2e-09

54.24

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Query:  EFLNKANPANLKVVIPATVERIIF----SGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        E LNK +P NL+V + A VE+IIF    SG++A GV Y+DS G  H AF+R  GE+ILS
Subjt:  EFLNKANPANLKVVIPATVERIIF----SGLSATGVSYSDSEGKLHTAFIRNKGEIILS

O82784 (R)-mandelonitrile lyase 4

2.1e-09

54.24

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Query:  EFLNKANPANLKVVIPATVERIIF----SGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        E LNK +P NLKV + A V++IIF    SGL+A GV Y+DS G  H A +  KGE+ILS
Subjt:  EFLNKANPANLKVVIPATVERIIF----SGLSATGVSYSDSEGKLHTAFIRNKGEIILS

P52706 (R)-mandelonitrile lyase 1

1.1e-10

57.58

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Query:  KGT--GAVEFLNKANPANLKVVIPATVERIIFS---GLSATGVSYSDSEGKLHTAFIRNKGEIILS
        KGT   A E LNK N  NL+V + A+VE+IIFS   GL+ATGV Y DS G  H AF+R+KGE+I+S
Subjt:  KGT--GAVEFLNKANPANLKVVIPATVERIIFS---GLSATGVSYSDSEGKLHTAFIRNKGEIILS

P52707 (R)-mandelonitrile lyase 3

5.5e-10

54.24

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Query:  EFLNKANPANLKVVIPATVERIIF----SGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        E LNK +P NL+V + A VE+IIF    SG++A GV Y+DS G  H AF+R +GE+ILS
Subjt:  EFLNKANPANLKVVIPATVERIIF----SGLSATGVSYSDSEGKLHTAFIRNKGEIILS

Q945K2 (R)-mandelonitrile lyase 2

1.1e-10

57.58

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Query:  KGT--GAVEFLNKANPANLKVVIPATVERIIFS---GLSATGVSYSDSEGKLHTAFIRNKGEIILS
        KGT   A E LNK N  NL+V + A+VE+IIFS   GL+ATGV Y DS G  H AF+R+KGE+I+S
Subjt:  KGT--GAVEFLNKANPANLKVVIPATVERIIFS---GLSATGVSYSDSEGKLHTAFIRNKGEIILS

Arabidopsis top hits

e value

%identity

Alignment

AT1G72970.1 Glucose-methanol-choline (GMC) oxidoreductase family protein

4.2e-05

44.44

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Query:  AVEFLNKANPANLKVVIPATVERIIF--SGL--SATGVSYSDSEGKLHTAFIRNK--GEIILS
        A E L  ANP  L+V+I ATV++I+F  SG     TGV + D +G  H A + N+   E+ILS
Subjt:  AVEFLNKANPANLKVVIPATVERIIF--SGL--SATGVSYSDSEGKLHTAFIRNK--GEIILS

AT1G72970.2 Glucose-methanol-choline (GMC) oxidoreductase family protein

4.2e-05

44.44

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Query:  AVEFLNKANPANLKVVIPATVERIIF--SGL--SATGVSYSDSEGKLHTAFIRNK--GEIILS
        A E L  ANP  L+V+I ATV++I+F  SG     TGV + D +G  H A + N+   E+ILS
Subjt:  AVEFLNKANPANLKVVIPATVERIIF--SGL--SATGVSYSDSEGKLHTAFIRNK--GEIILS

AT1G73050.1 Glucose-methanol-choline (GMC) oxidoreductase family protein

1.0e-06

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Query:  AVEFLNKANPANLKVVIPATVERIIF--------SGLSATGVSYSDSEGKLHTAFIRNKGEIILS
        + + L  A  +N++V + ATVER++         S +SA GV Y D  G+ H A IR++GE+ILS
Subjt:  AVEFLNKANPANLKVVIPATVERIIF--------SGLSATGVSYSDSEGKLHTAFIRNKGEIILS

Sequences

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CDS sequence

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ATGGAAAAGGGAACTGGAGCTGTGGAGTTTCTCAATAAGGCTAATCCTGCAAATCTTAAAGTTGTCATCCCGGCTACAGTTGAAAGAATCATTTTCTCTGGTTTATCTGC
AACTGGAGTTTCATATTCCGATTCAGAAGGAAAGTTGCACACAGCATTCATACGCAACAAAGGAGAGATTATTCTAAGCTGGAACCATTGGGAGTCCACAGCTTCTCCTC
CTAAGTGGGATTGGCCCAAAATCTCAACTTTCATCCTTAAAACTACCTGTCGTCCTGCACCAACCATACGTCGGCGAATTCACGTCCGACAATCCCCGTTTCGGCGCCAG
CATCGTGCTTCCATTCCCACTGGTCCCTTCATCGGGAAAAGTGGTCGGAACTTTGAAGAACAATATCCATTTGCAATCCTTCGCTAG

mRNA sequence

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Protein sequence

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MEKGTGAVEFLNKANPANLKVVIPATVERIIFSGLSATGVSYSDSEGKLHTAFIRNKGEIILSWNHWESTASPPKWDWPKISTFILKTTCRPAPTIRRRIHVRQSPFRRQ
HRASIPTGPFIGKSGRNFEEQYPFAILR

Moc08g40070 (gene) of Bitter gourd (OHB3-1) v2 genome