CuGenDBv2

Gene ID	PI0003129
Organism	Cucumis metuliferus PI 482460 (Melon (PI 482460) v1)
Description	Reverse transcriptase
Genome location	chr10:3639834..3640307
RNA-Seq Expression	PI0003129
Synteny	PI0003129
Gene Ontology terms	GO:0006278 - RNA-dependent DNA biosynthetic process (biological process) GO:0006355 - regulation of transcription, DNA-templated (biological process) GO:0006508 - proteolysis (biological process) GO:0015074 - DNA integration (biological process) GO:0090305 - nucleic acid phosphodiester bond hydrolysis (biological process) GO:0016021 - integral component of membrane (cellular component) GO:0003700 - DNA-binding transcription factor activity (molecular function) GO:0003964 - RNA-directed DNA polymerase activity (molecular function) GO:0004190 - aspartic-type endopeptidase activity (molecular function) GO:0004519 - endonuclease activity (molecular function) GO:0008270 - zinc ion binding (molecular function) GO:0043565 - sequence-specific DNA binding (molecular function)
InterPro domains	IPR000477 - Reverse transcriptase domain IPR043128 - Reverse transcriptase/Diguanylate cyclase domain IPR043502 - DNA/RNA polymerase superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0036671.1 gag protease polyprotein [Cucumis melo var. makuwa]

3.7e-68

82.8

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Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        MSFGLTNAP VFM+LMNRVF++FLDTFVIVFIDDIL+YSKTE EHE+HLRMVL+TL  N++YAKFSKCEFWL+QVSFLGHVVSK GV VDPAKI+AVT W
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK
        +RPS VSEVRSFLGLADYY RFVE+FSRIA+PL QLTRKGT FVWSK C+DSFQ LK
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK

KAA0046014.1 gag protease polyprotein [Cucumis melo var. makuwa]

6.3e-68

82.17

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Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        MSFGLTNAP VFM+LMNRVF++FLDTFVIVFIDDIL+YSKTE EHE+HLRMVL+TL  N++YAKFSKCEFWL+QVSFLGHVVSKDGV VDPAKI+AVT W
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK
        +RPS VSEVRSFLGLA YY RFVE+FSRIA+PL QLTRKG  FVWSK C+DSFQ LK
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK

KAA0046094.1 gag protease polyprotein [Cucumis melo var. makuwa]

2.4e-67

82.17

Show/hide

Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        MSFGLTNAP VFMNLMNRVF++FLDTFVIVFIDDIL+YSKTE EHE+HLRMVL+TL  N++YAKFSKCEFWL+QVSFLGHVVSK GV VDPAKI+AVT W
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK
        +RPS VSEVRSFLGLA YY RFVE+FSRIA+PL QLTRKG  FVWSK C+DSFQ LK
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK

KAA0057168.1 pol protein [Cucumis melo var. makuwa]

3.7e-68

82.8

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Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        MSFGLTNAP VFM+LMNRVFKDFLD+FVIVFIDDIL+YSKTE EHE+HLR VLETL AN++YAKFSKCEFWL++VSFLGHVVS +GV VDPAKI+AVTNW
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK
         RPS VSE+RSFLGLA YY RFVEDFSRIASPL QLTRKGT FVWS TC++SFQ LK
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK

KAA0060847.1 pol protein [Cucumis melo var. makuwa]

6.3e-68

82.17

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Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        MSFGLTNAP VFM+LMNRVF++FLDTFVIVFIDDIL+YSKTE EHE+HLRMVL+TL  N++YAKFSKCEFWL+QVSFLGHVVSKDGV VDPAKI+AVT W
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK
        +RPS VSEVRSFLGLA YY RFVE+FSRIA+PL QLTRKG  FVWSK C+DSFQ LK
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK

TrEMBL top hits

e value

%identity

Alignment

A0A5A7T538 Reverse transcriptase

1.8e-68

82.8

Show/hide

Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        MSFGLTNAP VFM+LMNRVF++FLDTFVIVFIDDIL+YSKTE EHE+HLRMVL+TL  N++YAKFSKCEFWL+QVSFLGHVVSK GV VDPAKI+AVT W
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK
        +RPS VSEVRSFLGLADYY RFVE+FSRIA+PL QLTRKGT FVWSK C+DSFQ LK
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK

A0A5A7TSI1 Gag protease polyprotein

3.1e-68

82.17

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Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        MSFGLTNAP VFM+LMNRVF++FLDTFVIVFIDDIL+YSKTE EHE+HLRMVL+TL  N++YAKFSKCEFWL+QVSFLGHVVSKDGV VDPAKI+AVT W
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK
        +RPS VSEVRSFLGLA YY RFVE+FSRIA+PL QLTRKG  FVWSK C+DSFQ LK
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK

A0A5A7TSQ8 Reverse transcriptase

1.2e-67

82.17

Show/hide

Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        MSFGLTNAP VFMNLMNRVF++FLDTFVIVFIDDIL+YSKTE EHE+HLRMVL+TL  N++YAKFSKCEFWL+QVSFLGHVVSK GV VDPAKI+AVT W
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK
        +RPS VSEVRSFLGLA YY RFVE+FSRIA+PL QLTRKG  FVWSK C+DSFQ LK
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK

A0A5A7UPX4 Pol protein

1.8e-68

82.8

Show/hide

Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        MSFGLTNAP VFM+LMNRVFKDFLD+FVIVFIDDIL+YSKTE EHE+HLR VLETL AN++YAKFSKCEFWL++VSFLGHVVS +GV VDPAKI+AVTNW
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK
         RPS VSE+RSFLGLA YY RFVEDFSRIASPL QLTRKGT FVWS TC++SFQ LK
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK

A0A5A7V1X7 Pol protein

3.1e-68

82.17

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Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        MSFGLTNAP VFM+LMNRVF++FLDTFVIVFIDDIL+YSKTE EHE+HLRMVL+TL  N++YAKFSKCEFWL+QVSFLGHVVSKDGV VDPAKI+AVT W
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK
        +RPS VSEVRSFLGLA YY RFVE+FSRIA+PL QLTRKG  FVWSK C+DSFQ LK
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK

SwissProt top hits

e value

%identity

Alignment

P04323 Retrovirus-related Pol polyprotein from transposon 17.6

2.0e-24

37.41

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Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        M FGL NAP  F   MN + +  L+   +V++DDI+V+S +  EH   L +V E L    +  +  KCEF  ++ +FLGHV++ DG+  +P KI+A+  +
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRK
          P+   E+++FLGL  YY +F+ +F+ IA P+ +  +K
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRK

P0CT41 Transposon Tf2-12 polyprotein

3.0e-20

31.21

Show/hide

Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        M +G++ AP  F   +N +  +  ++ V+ ++DDIL++SK+E EH  H++ VL+ L    +    +KCEF   QV F+G+ +S+ G       I  V  W
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK
         +P    E+R FLG  +Y  +F+   S++  PL  L +K   + W+ T   + + +K
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK

P10401 Retrovirus-related Pol polyprotein from transposon gypsy

2.2e-23

36.23

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Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        + FGL NA ++F   ++ V ++ +     V++DD++++S+ E +H  H+  VL+ L    +     K  F+   V +LG +VSKDG   DP K+KA+  +
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTR
          P  V +VRSFLGLA YY  F++DF+ IA P+  + +
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTR

P20825 Retrovirus-related Pol polyprotein from transposon 297

2.4e-25

35.46

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Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        M FGL NAP  F   MN + +  L+   +V++DDI+++S +  EH + +++V   L    +  +  KCEF  ++ +FLGH+V+ DG+  +P K+KA+ ++
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGT
          P+   E+R+FLGL  YY +F+ +++ IA P+    +K T
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGT

Q8I7P9 Retrovirus-related Pol polyprotein from transposon opus

6.9e-25

36.23

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Query:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW
        + FGL NAP +F  +++ + ++ +     V+IDDI+V+S+    H  +LR+VL +L    +     K  F   QV FLG++V+ DG+  DP K++A++  
Subjt:  MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNW

Query:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTR
          P+ V E++ FLG+  YY +F++D++++A PL  LTR
Subjt:  SRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTR

Arabidopsis top hits

e value

%identity

Alignment

ATMG00860.1 DNA/RNA polymerases superfamily protein

4.0e-20

40.71

Show/hide

Query:  DHLRMVLETLCANQVYAKFSKCEFWLRQVSFLG--HVVSKDGVYVDPAKIKAVTNWSRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFV
        +HL MVL+    +Q YA   KC F   Q+++LG  H++S +GV  DPAK++A+  W  P   +E+R FLGL  YY RFV+++ +I  PL +L +K  S  
Subjt:  DHLRMVLETLCANQVYAKFSKCEFWLRQVSFLG--HVVSKDGVYVDPAKIKAVTNWSRPSIVSEVRSFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFV

Query:  WSKTCKDSFQILK
        W++    +F+ LK
Subjt:  WSKTCKDSFQILK

Sequences

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CDS sequence

Show/hide CDS sequence

ATGTCTTTTGGGTTGACAAATGCTCCAACAGTGTTTATGAACTTGATGAACAGGGTGTTCAAGGATTTCTTAGACACTTTTGTGATTGTGTTCATTGACGATATCTTGGT
TTACTCCAAGACAGAGGTAGAGCATGAGGATCATTTACGCATGGTCTTAGAGACTCTTTGTGCCAACCAGGTGTATGCTAAGTTCTCGAAATGTGAGTTTTGGCTGAGGC
AAGTGTCTTTCCTAGGCCATGTGGTGTCTAAAGATGGTGTTTATGTGGACCCAGCAAAGATCAAAGCGGTTACCAATTGGTCTCGACCTTCTATAGTCAGCGAGGTTCGT
AGTTTTCTGGGTCTAGCAGATTATTACCACCGATTTGTGGAGGACTTTTCTCGTATTGCTTCTCCTTTGGCCCAGTTGACCAGAAAGGGGACCTCATTTGTTTGGAGCAA
AACTTGTAAGGATAGTTTTCAGATTCTCAAATAG

mRNA sequence

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Protein sequence

Show/hide protein sequence

MSFGLTNAPTVFMNLMNRVFKDFLDTFVIVFIDDILVYSKTEVEHEDHLRMVLETLCANQVYAKFSKCEFWLRQVSFLGHVVSKDGVYVDPAKIKAVTNWSRPSIVSEVR
SFLGLADYYHRFVEDFSRIASPLAQLTRKGTSFVWSKTCKDSFQILK

PI0003129 (gene) of Melon (PI 482460) v1 genome