CuGenDBv2

Gene ID	PI0008402
Organism	Cucumis metuliferus PI 482460 (Melon (PI 482460) v1)
Description	Reverse transcriptase
Genome location	chr01:21836141..21836602
RNA-Seq Expression	PI0008402
Synteny	PI0008402
Gene Ontology terms	GO:0006278 - RNA-dependent DNA biosynthetic process (biological process) GO:0015074 - DNA integration (biological process) GO:0003676 - nucleic acid binding (molecular function) GO:0003964 - RNA-directed DNA polymerase activity (molecular function) GO:0008270 - zinc ion binding (molecular function)
InterPro domains	IPR000477 - Reverse transcriptase domain IPR043128 - Reverse transcriptase/Diguanylate cyclase domain IPR043502 - DNA/RNA polymerase superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0032755.1 reverse transcriptase [Cucumis melo var. makuwa]

1.8e-67

83.01

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MPFGLTN+P  FMDLMN+IFH+YLDQFVIVFIDDILVYS+D+++HEEHLRIVLQTLREKQLYAK +KCEFWL Q+VFLGHVVSA GVS+DPQKV  +VNW
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF
        ERP SATEVRSF GL GYYRRFIEDFSR ALPLTALTRKN KF+ SDK+EQSF
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF

KAA0050493.1 DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]

5.2e-67

83.66

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MPFGLTN+P  FMDLMN+IFH+YLDQFVIVFIDDILVYS+D+++HEEHLRIVLQTLREKQLYAK +KCEFWL Q+VFLGHVVSA GVS+DPQKV  VVNW
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF
        ERP SATEVRSF GL GYYRRFIEDFSR ALPLTALTRKN KF+ SDK EQSF
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF

TYJ96975.1 DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]

3.1e-67

83.66

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MPFGLTN+P  FMDLMN+IFHQYLDQFVIVFIDDILVYS+D+++HEEHLRIVLQTLR+KQLYAK +KCEFWL Q+VFLGHVVSA GVS+DPQKV  VVNW
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF
        ERP SATEVRSF GL GYYRRFIEDFS+ ALPLTALTRKNAKF+ SDK EQSF
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF

TYK00847.1 DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]

2.1e-68

85.62

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MPFGL N+P  FMDLMN+IFHQYLDQFVI+FIDDILVYS+DKKAHEEHLRIVLQTL +KQLYAK NKCEFWLNQ+VFLGHVVSA GVS+DPQKV VVVNW
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF
        ERPASATEVRSF GL GYYRRF+EDFSR ALPLTALTRKNAK++ SDK EQSF
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF

TYK05031.1 reverse transcriptase [Cucumis melo var. makuwa]

4.0e-67

84.97

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MPFGLTN+P+ FM+LMN+IFHQYLDQFVIVFIDDILVYS+DKKAHEEHLRIVLQTLR+KQLYAK +KCE WLNQ+VFLGHVVSADGVS+DPQKV  V NW
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF
        ERP SATEV SF GL GYYRRFIEDFSR ALPLTALTRKNAKF+ SDK EQSF
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF

TrEMBL top hits

e value

%identity

Alignment

A0A5A7SU37 Reverse transcriptase

8.7e-68

83.01

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MPFGLTN+P  FMDLMN+IFH+YLDQFVIVFIDDILVYS+D+++HEEHLRIVLQTLREKQLYAK +KCEFWL Q+VFLGHVVSA GVS+DPQKV  +VNW
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF
        ERP SATEVRSF GL GYYRRFIEDFSR ALPLTALTRKN KF+ SDK+EQSF
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF

A0A5A7U2V7 Reverse transcriptase

2.5e-67

83.66

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MPFGLTN+P  FMDLMN+IFH+YLDQFVIVFIDDILVYS+D+++HEEHLRIVLQTLREKQLYAK +KCEFWL Q+VFLGHVVSA GVS+DPQKV  VVNW
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF
        ERP SATEVRSF GL GYYRRFIEDFSR ALPLTALTRKN KF+ SDK EQSF
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF

A0A5D3BEU1 DNA/RNA polymerases superfamily protein

1.5e-67

83.66

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MPFGLTN+P  FMDLMN+IFHQYLDQFVIVFIDDILVYS+D+++HEEHLRIVLQTLR+KQLYAK +KCEFWL Q+VFLGHVVSA GVS+DPQKV  VVNW
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF
        ERP SATEVRSF GL GYYRRFIEDFS+ ALPLTALTRKNAKF+ SDK EQSF
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF

A0A5D3BNL5 DNA/RNA polymerases superfamily protein

1.0e-68

85.62

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MPFGL N+P  FMDLMN+IFHQYLDQFVI+FIDDILVYS+DKKAHEEHLRIVLQTL +KQLYAK NKCEFWLNQ+VFLGHVVSA GVS+DPQKV VVVNW
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF
        ERPASATEVRSF GL GYYRRF+EDFSR ALPLTALTRKNAK++ SDK EQSF
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF

A0A5D3BZC5 Reverse transcriptase

1.9e-67

84.97

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MPFGLTN+P+ FM+LMN+IFHQYLDQFVIVFIDDILVYS+DKKAHEEHLRIVLQTLR+KQLYAK +KCE WLNQ+VFLGHVVSADGVS+DPQKV  V NW
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF
        ERP SATEV SF GL GYYRRFIEDFSR ALPLTALTRKNAKF+ SDK EQSF
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF

SwissProt top hits

e value

%identity

Alignment

P04323 Retrovirus-related Pol polyprotein from transposon 17.6

3.6e-26

37.01

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MPFGL N+P  F   MN I    L++  +V++DDI+V+S     H + L +V + L +  L  +L+KCEF   +  FLGHV++ DG+  +P+K+  +  +
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSD-KYEQSF
          P    E+++F GL GYYR+FI +F+  A P+T   +KN K   ++ +Y+ +F
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSD-KYEQSF

P0CT34 Transposon Tf2-1 polyprotein

5.0e-20

31.58

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MP+G++ +P  F   +N I  +  +  V+ ++DDIL++S  +  H +H++ VLQ L+   L     KCEF  +Q+ F+G+ +S  G +   + +  V+ W
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQS
        ++P +  E+R F G V Y R+FI   S+   PL  L +K+ ++K +    Q+
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQS

P0CT41 Transposon Tf2-12 polyprotein

5.0e-20

31.58

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MP+G++ +P  F   +N I  +  +  V+ ++DDIL++S  +  H +H++ VLQ L+   L     KCEF  +Q+ F+G+ +S  G +   + +  V+ W
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQS
        ++P +  E+R F G V Y R+FI   S+   PL  L +K+ ++K +    Q+
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQS

P20825 Retrovirus-related Pol polyprotein from transposon 297

5.1e-25

38.03

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        MPFGL N+P  F   MN I    L++  +V++DDI+++S     H   +++V   L +  L  +L+KCEF   +  FLGH+V+ DG+  +P KV  +V++
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAK
          P    E+R+F GL GYYR+FI +++  A P+T+  +K  K
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNAK

Q8I7P9 Retrovirus-related Pol polyprotein from transposon opus

2.1e-26

40.58

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Query:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW
        +PFGL N+P  F  +++ I  +++ +   V+IDDI+V+S D   H ++LR+VL +L +  L   L K  F   Q+ FLG++V+ADG+  DP+KV  +   
Subjt:  MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNW

Query:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTR
          P S  E++ F G+  YYR+FI+D+++ A PLT LTR
Subjt:  ERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTR

Arabidopsis top hits

e value

%identity

Alignment

ATMG00860.1 DNA/RNA polymerases superfamily protein

3.0e-20

45.83

Show/hide

Query:  HLRIVLQTLREKQLYAKLNKCEFWLNQIVFLG--HVVSADGVSIDPQKVGVVVNWERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNA
        HL +VLQ   + Q YA   KC F   QI +LG  H++S +GVS DP K+  +V W  P + TE+R F GL GYYRRF++++ +   PLT L +KN+
Subjt:  HLRIVLQTLREKQLYAKLNKCEFWLNQIVFLG--HVVSADGVSIDPQKVGVVVNWERPASATEVRSFQGLVGYYRRFIEDFSRSALPLTALTRKNA

Sequences

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CDS sequence

Show/hide CDS sequence

ATGCCATTCGGATTAACGAATTCGCCAAAATTTTTCATGGATCTCATGAACAAGATCTTCCATCAATATTTAGATCAGTTCGTGATAGTGTTCATTGATGACATACTAGT
GTACTCGATTGATAAGAAAGCCCATGAGGAACATCTAAGGATTGTTCTACAAACGCTACGTGAAAAACAGTTGTATGCCAAGTTGAACAAATGTGAGTTTTGGTTGAACC
AGATAGTGTTCTTAGGGCATGTAGTTTCAGCGGACGGGGTTAGTATTGATCCGCAAAAAGTTGGAGTTGTTGTCAACTGGGAGAGACCAGCTAGTGCGACAGAAGTACGT
AGTTTCCAAGGCTTGGTAGGATATTACAGACGTTTTATTGAGGATTTCTCACGATCAGCATTACCCTTAACAGCTTTGACAAGGAAGAATGCTAAGTTTAAGTGCTCAGA
TAAATATGAACAGAGTTTCTAG

mRNA sequence

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Protein sequence

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MPFGLTNSPKFFMDLMNKIFHQYLDQFVIVFIDDILVYSIDKKAHEEHLRIVLQTLREKQLYAKLNKCEFWLNQIVFLGHVVSADGVSIDPQKVGVVVNWERPASATEVR
SFQGLVGYYRRFIEDFSRSALPLTALTRKNAKFKCSDKYEQSF

PI0008402 (gene) of Melon (PI 482460) v1 genome