CuGenDBv2

Gene ID	PI0009123
Organism	Cucumis metuliferus PI 482460 (Melon (PI 482460) v1)
Description	PseudoU_synth_1 domain-containing protein
Genome location	chr12:24615508..24616182
RNA-Seq Expression	PI0009123
Synteny	PI0009123
Gene Ontology terms	GO:0031119 - tRNA pseudouridine synthesis (biological process) GO:0003723 - RNA binding (molecular function) GO:0009982 - pseudouridine synthase activity (molecular function)
InterPro domains	IPR001406 - Pseudouridine synthase I, TruA IPR020095 - Pseudouridine synthase I, TruA, C-terminal

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0059994.1 putative tRNA pseudouridine synthase [Cucumis melo var. makuwa]

3.8e-77

86.36

Show/hide

Query:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE
        MIGTAVAVKRKLLPRD+IALSLTKFSRIVLPIAPAEVLVLRGNSFS RKRPGVPTRPDLVTLNESDDI+KSVNEFYRSAILPEVT+FLDTESPPWKEWVE
Subjt:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE

Query:  KLDKYTSIPNEELDEVRSAWKAWK------------GVINGINYGNWALNGRGLWSPTSRWAFNRAASTADNQLGT
        KLDKYTSIPNEELDEVRSAWKAWK            GVIN I+YGNWALNG GLW PT R  FN AASTADNQLGT
Subjt:  KLDKYTSIPNEELDEVRSAWKAWK------------GVINGINYGNWALNGRGLWSPTSRWAFNRAASTADNQLGT

XP_008457308.1 PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo]

8.4e-61

96.77

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Query:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE
        MIGTAVAVKRKLLPRD+IALSLTKFSRIVLPIAPAEVLVLRGNSFS RKRPGVPTRPDLVTLNESDDI+KSVNEFYRSAILPEVT+FLDTESPPWKEWVE
Subjt:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE

Query:  KLDKYTSIPNEELDEVRSAWKAWK
        KLDKYTSIPNEELDEVRSAWKAWK
Subjt:  KLDKYTSIPNEELDEVRSAWKAWK

XP_031742713.1 putative tRNA pseudouridine synthase isoform X1 [Cucumis sativus]

2.5e-57

93.55

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Query:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE
        MI TAVAVKRKLLPRDII LSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPG P R +L TLNESDDIV SVNEFYRSAILPEVTKFLDTESPPWKEWVE
Subjt:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE

Query:  KLDKYTSIPNEELDEVRSAWKAWK
        KLDKYTSIPNEELDEVRSAWKAWK
Subjt:  KLDKYTSIPNEELDEVRSAWKAWK

XP_038894774.1 putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida]

2.5e-57

91.87

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Query:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE
        MIGTA+AVKRKLLPRD+IALSL+KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVP RPDL+TLNESDDIVKSVNEFYRSAILPEVT FLDTE PPWKEWVE
Subjt:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE

Query:  KLDKYTSIPNEELDEVRSAWKAW
        KLDKYTSIP+E+LDEVR+AWKAW
Subjt:  KLDKYTSIPNEELDEVRSAWKAW

XP_038894775.1 putative tRNA pseudouridine synthase isoform X2 [Benincasa hispida]

2.5e-57

91.87

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Query:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE
        MIGTA+AVKRKLLPRD+IALSL+KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVP RPDL+TLNESDDIVKSVNEFYRSAILPEVT FLDTE PPWKEWVE
Subjt:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE

Query:  KLDKYTSIPNEELDEVRSAWKAW
        KLDKYTSIP+E+LDEVR+AWKAW
Subjt:  KLDKYTSIPNEELDEVRSAWKAW

TrEMBL top hits

e value

%identity

Alignment

A0A0A0M0Z2 PseudoU_synth_1 domain-containing protein

1.2e-57

93.55

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Query:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE
        MI TAVAVKRKLLPRDII LSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPG P R +L TLNESDDIV SVNEFYRSAILPEVTKFLDTESPPWKEWVE
Subjt:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE

Query:  KLDKYTSIPNEELDEVRSAWKAWK
        KLDKYTSIPNEELDEVRSAWKAWK
Subjt:  KLDKYTSIPNEELDEVRSAWKAWK

A0A1S3C5W1 putative tRNA pseudouridine synthase

4.1e-61

96.77

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Query:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE
        MIGTAVAVKRKLLPRD+IALSLTKFSRIVLPIAPAEVLVLRGNSFS RKRPGVPTRPDLVTLNESDDI+KSVNEFYRSAILPEVT+FLDTESPPWKEWVE
Subjt:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE

Query:  KLDKYTSIPNEELDEVRSAWKAWK
        KLDKYTSIPNEELDEVRSAWKAWK
Subjt:  KLDKYTSIPNEELDEVRSAWKAWK

A0A5A7UY60 Putative tRNA pseudouridine synthase

1.8e-77

86.36

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Query:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE
        MIGTAVAVKRKLLPRD+IALSLTKFSRIVLPIAPAEVLVLRGNSFS RKRPGVPTRPDLVTLNESDDI+KSVNEFYRSAILPEVT+FLDTESPPWKEWVE
Subjt:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE

Query:  KLDKYTSIPNEELDEVRSAWKAWK------------GVINGINYGNWALNGRGLWSPTSRWAFNRAASTADNQLGT
        KLDKYTSIPNEELDEVRSAWKAWK            GVIN I+YGNWALNG GLW PT R  FN AASTADNQLGT
Subjt:  KLDKYTSIPNEELDEVRSAWKAWK------------GVINGINYGNWALNGRGLWSPTSRWAFNRAASTADNQLGT

A0A6J1HM83 putative tRNA pseudouridine synthase

8.8e-56

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Query:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE
        M+GTA+AVKRKLLPRDIIALSL+KFSRIVLPIAPAEVLVLRGN FSNRKRPG P RPDL+TLNES DIVKSVNEFYRSAILPEVT FLD E PPWKEWVE
Subjt:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE

Query:  KLDKYTSIPNEELDEVRSAWKAWKG
        KLDKYTSIPNE+L+EVR+AWKAW G
Subjt:  KLDKYTSIPNEELDEVRSAWKAWKG

A0A6J1I156 putative tRNA pseudouridine synthase

3.9e-56

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Query:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE
        M+GTA+AVKRKLLPRDIIALSL+KFSRIVLPIAPAEVLVLRGN FSNRKRPG P RPDL+TLN+SDDIVKSVNEFYRSAILPEVT FLD E PPWKEWVE
Subjt:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE

Query:  KLDKYTSIPNEELDEVRSAWKAWKG
        KLDKYTSIPNE+L+EVR+AWKAW G
Subjt:  KLDKYTSIPNEELDEVRSAWKAWKG

SwissProt top hits

e value

%identity

Alignment

O22928 Putative tRNA pseudouridine synthase

9.7e-36

57.26

Show/hide

Query:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE
        MIGTAVAVKR+LLPRDII LSL KF+RIVLP+AP+EVL+LRGNSF  R+   +P RP +    ES+++ K + EFYR+ ++P+V+ FLD+E  PWKEWV+
Subjt:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE

Query:  KLDKYTSIPNEELDEVRSAWKAWK
         LD+   + +EEL++VR  W+ WK
Subjt:  KLDKYTSIPNEELDEVRSAWKAWK

Arabidopsis top hits

e value

%identity

Alignment

AT2G30320.1 Pseudouridine synthase family protein

6.9e-37

57.26

Show/hide

Query:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE
        MIGTAVAVKR+LLPRDII LSL KF+RIVLP+AP+EVL+LRGNSF  R+   +P RP +    ES+++ K + EFYR+ ++P+V+ FLD+E  PWKEWV+
Subjt:  MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVE

Query:  KLDKYTSIPNEELDEVRSAWKAWK
         LD+   + +EEL++VR  W+ WK
Subjt:  KLDKYTSIPNEELDEVRSAWKAWK

Sequences

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CDS sequence

Show/hide CDS sequence

ATGATAGGAACGGCTGTAGCAGTGAAGCGGAAACTTCTCCCAAGAGATATTATAGCATTGTCACTGACCAAGTTCTCTAGAATAGTTCTACCCATTGCTCCAGCTGAAGT
CCTAGTCCTAAGAGGGAACAGTTTTTCCAACAGAAAACGGCCAGGAGTTCCAACACGGCCAGACTTGGTCACACTGAATGAATCAGACGACATAGTCAAGTCAGTGAATG
AATTCTACAGATCAGCAATATTGCCCGAGGTAACTAAGTTCCTGGACACAGAAAGCCCCCCATGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCGAAT
GAAGAATTGGATGAAGTTAGGAGTGCTTGGAAAGCATGGAAGGGAGTTATTAATGGCATAAATTATGGGAATTGGGCATTGAATGGACGTGGTCTGTGGTCACCCACCAG
CCGGTGGGCGTTTAATAGAGCAGCATCAACTGCAGACAATCAACTCGGGACTCGGCACAACCACAAGGGAGTGAGCTCGCATTCAATGTAA

mRNA sequence

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Protein sequence

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MIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPTRPDLVTLNESDDIVKSVNEFYRSAILPEVTKFLDTESPPWKEWVEKLDKYTSIPN
EELDEVRSAWKAWKGVINGINYGNWALNGRGLWSPTSRWAFNRAASTADNQLGTRHNHKGVSSHSM

PI0009123 (gene) of Melon (PI 482460) v1 genome