CuGenDBv2

Gene ID	PI0014744
Organism	Cucumis metuliferus PI 482460 (Melon (PI 482460) v1)
Description	DNA-directed RNA polymerase
Genome location	chr04:16185273..16186298
RNA-Seq Expression	PI0014744
Synteny	PI0014744
Gene Ontology terms	GO:0006390 - mitochondrial transcription (biological process) GO:0034245 - mitochondrial DNA-directed RNA polymerase complex (cellular component) GO:0003677 - DNA binding (molecular function) GO:0003899 - DNA-directed 5'-3' RNA polymerase activity (molecular function)
InterPro domains	IPR002092 - DNA-directed RNA polymerase, phage-type IPR043502 - DNA/RNA polymerase superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

GAV59073.1 RNA_pol domain-containing protein [Cephalotus follicularis]

3.1e-34

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQP-----PCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE DKI V+     +P     +  GS        CK AG     VHDSYWT ACDVDEMNRILREKFVELYEAPI ENLLE FQKSF T
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQP-----PCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        LKFPPLP++GDFDLKDVLQSPYFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

PON79803.1 DNA-directed RNA polymerase, phage-type [Parasponia andersonii]

1.5e-33

65.6

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE DK+ V+     +P     +  GS        CK AG     VHDSYWT ACDVDEMNRILREKFVELYEAPI ENLLE+FQKSF T
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        LKFPPLPD+GDFDL++VL+SPYFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

XP_022149021.1 DNA-directed RNA polymerase 1B, mitochondrial [Momordica charantia]

6.9e-34

67.2

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE D++ V+     +P     +  GS        CK AG     VHDSYWT ACDVDEMNRILREKFVELYEAPI ENLLENFQKSF T
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        LKFPPLPD+GDFDLKDVLQS YFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

XP_022967967.1 DNA-directed RNA polymerase 1B, mitochondrial-like [Cucurbita maxima]

1.2e-33

66.4

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE DK+  +     +P     +  GS        CK AG     VHDSYWT ACDVDEMNR+LREKFVELYEAPI ENLLENFQKSF T
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        LKFPPLPD+GDFDLKDVLQS YFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

XP_038892390.1 DNA-directed RNA polymerase 1B, mitochondrial [Benincasa hispida]

8.2e-35

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE DK+  +     +P     +  GS        CK AG     VHDSYWT ACDVDEMNRILREKFVELYEAPI ENLLENFQKSF T
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        LKFPPLPD+GDFDLKDVLQSPYFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

TrEMBL top hits

e value

%identity

Alignment

A0A1Q3AU35 DNA-directed RNA polymerase

1.5e-34

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQP-----PCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE DKI V+     +P     +  GS        CK AG     VHDSYWT ACDVDEMNRILREKFVELYEAPI ENLLE FQKSF T
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQP-----PCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        LKFPPLP++GDFDLKDVLQSPYFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

A0A2P5DTZ2 DNA-directed RNA polymerase

2.2e-33

64.8

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+L+RE DK+ V+     +P     +  GS        CK AG     VHDSYWT ACDVDEMNRILREKFVELYEAPI ENLLE+FQKSF T
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        LKFPPLPD+GDFDL++VL+SPYFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

A0A2P5E2P4 DNA-directed RNA polymerase

7.5e-34

65.6

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE DK+ V+     +P     +  GS        CK AG     VHDSYWT ACDVDEMNRILREKFVELYEAPI ENLLE+FQKSF T
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        LKFPPLPD+GDFDL++VL+SPYFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

A0A6J1D747 DNA-directed RNA polymerase

3.4e-34

67.2

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE D++ V+     +P     +  GS        CK AG     VHDSYWT ACDVDEMNRILREKFVELYEAPI ENLLENFQKSF T
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        LKFPPLPD+GDFDLKDVLQS YFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

A0A6J1HS83 DNA-directed RNA polymerase

5.7e-34

66.4

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE DK+  +     +P     +  GS        CK AG     VHDSYWT ACDVDEMNR+LREKFVELYEAPI ENLLENFQKSF T
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        LKFPPLPD+GDFDLKDVLQS YFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

SwissProt top hits

e value

%identity

Alignment

Q8L6J2 DNA-directed RNA polymerase 2A (Fragment)

3.9e-32

58.4

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE +K+ V+     +P     +  GS        C+ AG     VHDSYWT ACDVD++NRILREKFVELYEAPI E LLE+FQ S+ T
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        L FPPLP++GDFD++DVL+SPYFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

Q8L6J4 DNA-directed RNA polymerase 1A (Fragment)

5.2e-32

58.4

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQP-----PCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE DK+ V+     +P     +  GS        CK +G     VHDSYWT A DVD+MN+ILREKFVELY+API ENLLE+FQ+SF  
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQP-----PCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        L+FPPLP++GDFDL++VL+SPYFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

Q8L6J5 DNA-directed RNA polymerase 1B, mitochondrial

3.6e-33

59.2

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQP-----PCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE DK+ V+     +P     +  GS        CK +G     VHDSYWT ACDVD+MN+ILREKFVELY+API ENLLE+FQ+SF  
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQP-----PCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        L+FPPLP++GDFDL++VL+SPYFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

Q8VWF8 DNA-directed RNA polymerase 2, chloroplastic/mitochondrial

3.9e-32

58.4

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE +K+ V+     +P     +  GS        C+ AG     VHDSYWT ACDVD++NRILREKFVELYEAPI E LLE+FQ S+ T
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        L FPPLP++GDFD++DVL+SPYFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

Q93Y94 DNA-directed RNA polymerase 1, mitochondrial

5.2e-32

58.4

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQP-----PCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE DK+ V+     +P     +  GS        CK +G     VHDSYWT A DVD+MN+ILREKFVELY+API ENLLE+FQ+SF  
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQP-----PCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        L+FPPLP++GDFDL++VL+SPYFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

Arabidopsis top hits

e value

%identity

Alignment

AT1G68990.1 male gametophyte defective 3

9.3e-29

53.6

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        + T+LQ+L+L RE DK+        +      +  GS        C  AG     VHDS+WT ACDVD MN ILREKFVELYE PI ENLLE+FQKSF  
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        + FPPLP++GDFDL+ VL+S YFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

AT1G68990.2 male gametophyte defective 3

9.3e-29

53.6

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        + T+LQ+L+L RE DK+        +      +  GS        C  AG     VHDS+WT ACDVD MN ILREKFVELYE PI ENLLE+FQKSF  
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        + FPPLP++GDFDL+ VL+S YFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

AT2G24120.1 DNA/RNA polymerases superfamily protein

4.6e-28

53.6

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        I TSLQ+L+LQRE + + V      +P     +  G+        C+ AG     VHDSYWT ACDVD MNRILREKFVELY  PI E+LL++FQ+S+  
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGY-VLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        L FPP+P +GDFDLK+VL+S YFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

AT5G15700.1 DNA/RNA polymerases superfamily protein

2.5e-29

55.2

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        + TSLQ LSLQ E D++ V      +P     +  GS        CK AG     VHDS+WT ACDVD++N ILREKFVELY  PI ENLLE+F++SF  
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLKDVLQSPYFFN
        L FPPLP++GD DLK VL SPYFFN
Subjt:  LKFPPLPDQGDFDLKDVLQSPYFFN

AT5G15700.2 DNA/RNA polymerases superfamily protein

1.1e-24

50.82

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Query:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST
        + TSLQ LSLQ E D++ V      +P     +  GS        CK AG     VHDS+WT ACDVD++N ILREKFVELY  PI ENLLE+F++SF  
Subjt:  IGTSLQLLSLQREIDKIFVE-----YPTKLRKAEKGSQ-----PPCKGAGYVLR-VHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQKSFST

Query:  LKFPPLPDQGDFDLK-DVLQSP
        L FPPLP++GD DLK +++ +P
Subjt:  LKFPPLPDQGDFDLK-DVLQSP

Sequences

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CDS sequence

Show/hide CDS sequence

ATGGACCACTCTTCTTGGATTGCCAGTGGTGCAACCTTATTGGCAACTAGGAAAGCTGTACTTGACTTGTTGCAGATTGGGACTTCCTTGCAATTGTTGTCTCTACAACG
AGAGATTGACAAGATTTTTGTTGAATACCCCACAAAATTGCGCAAGGCAGAGAAGGGTTCCCAGCCTCCTTGTAAAGGTGCTGGGTATGTTCTTAGAGTCCATGATTCAT
ATTGGACCGATGCATGTGATGTCGACGAAATGAATAGAATACTTAGGGAGAAGTTCGTTGAGCTATATGAGGCTCCTATTCCGGAGAATTTATTGGAGAACTTCCAAAAG
TCTTTCTCCACGTTGAAATTTCCTCCCTTACCTGATCAGGGAGACTTCGATCTCAAAGATGTTCTGCAGTCTCCCTATTTCTTCAATTAG

mRNA sequence

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Protein sequence

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MDHSSWIASGATLLATRKAVLDLLQIGTSLQLLSLQREIDKIFVEYPTKLRKAEKGSQPPCKGAGYVLRVHDSYWTDACDVDEMNRILREKFVELYEAPIPENLLENFQK
SFSTLKFPPLPDQGDFDLKDVLQSPYFFN

PI0014744 (gene) of Melon (PI 482460) v1 genome