CuGenDBv2

Gene ID	PI0015487
Organism	Cucumis metuliferus PI 482460 (Melon (PI 482460) v1)
Description	Reverse transcriptase
Genome location	chr03:23299980..23300450
RNA-Seq Expression	PI0015487
Synteny	PI0015487
Gene Ontology terms	GO:0006278 - RNA-dependent DNA biosynthetic process (biological process) GO:0006355 - regulation of transcription, DNA-templated (biological process) GO:0006508 - proteolysis (biological process) GO:0015074 - DNA integration (biological process) GO:0090305 - nucleic acid phosphodiester bond hydrolysis (biological process) GO:0003700 - DNA-binding transcription factor activity (molecular function) GO:0003964 - RNA-directed DNA polymerase activity (molecular function) GO:0004190 - aspartic-type endopeptidase activity (molecular function) GO:0004519 - endonuclease activity (molecular function) GO:0008270 - zinc ion binding (molecular function) GO:0043565 - sequence-specific DNA binding (molecular function)
InterPro domains	IPR000477 - Reverse transcriptase domain IPR043128 - Reverse transcriptase/Diguanylate cyclase domain IPR043502 - DNA/RNA polymerase superfamily

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAA0036538.1 pol protein [Cucumis melo var. makuwa]

4.7e-71

85.99

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        MSFGLTNAPTVFMDLMNRVFKDFLD+FVIVFIDDIL+YSKTEAEHE HL +VLETLRAN+L+AKFSKCEFWLR+V+FLGHVVS + V VDPAKIEAVTNW
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK
         RPS++ E+RSFLGLAGYYRRFVE+FSRIASPLTQLTRKGTPFVWS ACE SFQ+LK
Subjt:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK

KAA0046014.1 gag protease polyprotein [Cucumis melo var. makuwa]

7.2e-72

86.62

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        MSFGLTNAP VFMDLMNRVF++FLDTFVIVFIDDIL+YSKTEAEHE HLR VL+TLR N+L+AKFSKCEFWL+QVSFLGHVVSKDGV VDPAKIEAVT W
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK
        +RPS++ EVRSFLGLAGYYRRFVENFSRIA+PLTQLTRKG PFVWS+ACEDSFQ LK
Subjt:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK

KAA0059792.1 pol protein [Cucumis melo var. makuwa]

9.4e-72

86.62

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        MSFGLTNAPTVFMDLMNRVF++FLDTFVIVFIDDIL+YSKTEAEHE HLRKVL+TLR N+L+AKFSKCEFWL+QVSFLGHV+SK GV VDPAKIEAVT W
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK
        +RPS++ EVRSFLGLAGYYRRFVENFSRIA+PLTQLTRKG PFVWS+ACEDSFQ LK
Subjt:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK

KAA0060847.1 pol protein [Cucumis melo var. makuwa]

7.2e-72

86.62

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        MSFGLTNAP VFMDLMNRVF++FLDTFVIVFIDDIL+YSKTEAEHE HLR VL+TLR N+L+AKFSKCEFWL+QVSFLGHVVSKDGV VDPAKIEAVT W
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK
        +RPS++ EVRSFLGLAGYYRRFVENFSRIA+PLTQLTRKG PFVWS+ACEDSFQ LK
Subjt:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK

KAA0062112.1 pol protein [Cucumis melo var. makuwa]

1.6e-71

86.62

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        MSFGLTNAP VFMDLMNRVF++FLDTFVIVFIDDIL+YSKTEAEHENHLR VL+TLR N+L+AKFSKCEFWL+QVSFLGHVVSK GV VDPAKIEAVT W
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK
        +RPS++ EVRSFLGLAGYYRRFVENFSRIA+PLTQLTRKG PFVWS+ACEDSFQ LK
Subjt:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK

TrEMBL top hits

e value

%identity

Alignment

A0A5A7T165 Reverse transcriptase

2.3e-71

85.99

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        MSFGLTNAPTVFMDLMNRVFKDFLD+FVIVFIDDIL+YSKTEAEHE HL +VLETLRAN+L+AKFSKCEFWLR+V+FLGHVVS + V VDPAKIEAVTNW
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK
         RPS++ E+RSFLGLAGYYRRFVE+FSRIASPLTQLTRKGTPFVWS ACE SFQ+LK
Subjt:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK

A0A5A7TSI1 Gag protease polyprotein

3.5e-72

86.62

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        MSFGLTNAP VFMDLMNRVF++FLDTFVIVFIDDIL+YSKTEAEHE HLR VL+TLR N+L+AKFSKCEFWL+QVSFLGHVVSKDGV VDPAKIEAVT W
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK
        +RPS++ EVRSFLGLAGYYRRFVENFSRIA+PLTQLTRKG PFVWS+ACEDSFQ LK
Subjt:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK

A0A5A7UV42 Reverse transcriptase

4.5e-72

86.62

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        MSFGLTNAPTVFMDLMNRVF++FLDTFVIVFIDDIL+YSKTEAEHE HLRKVL+TLR N+L+AKFSKCEFWL+QVSFLGHV+SK GV VDPAKIEAVT W
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK
        +RPS++ EVRSFLGLAGYYRRFVENFSRIA+PLTQLTRKG PFVWS+ACEDSFQ LK
Subjt:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK

A0A5A7V1N3 Reverse transcriptase

7.8e-72

86.62

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        MSFGLTNAP VFMDLMNRVF++FLDTFVIVFIDDIL+YSKTEAEHENHLR VL+TLR N+L+AKFSKCEFWL+QVSFLGHVVSK GV VDPAKIEAVT W
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK
        +RPS++ EVRSFLGLAGYYRRFVENFSRIA+PLTQLTRKG PFVWS+ACEDSFQ LK
Subjt:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK

A0A5A7V1X7 Pol protein

3.5e-72

86.62

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        MSFGLTNAP VFMDLMNRVF++FLDTFVIVFIDDIL+YSKTEAEHE HLR VL+TLR N+L+AKFSKCEFWL+QVSFLGHVVSKDGV VDPAKIEAVT W
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK
        +RPS++ EVRSFLGLAGYYRRFVENFSRIA+PLTQLTRKG PFVWS+ACEDSFQ LK
Subjt:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK

SwissProt top hits

e value

%identity

Alignment

P04323 Retrovirus-related Pol polyprotein from transposon 17.6

8.1e-26

41.73

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        M FGL NAP  F   MN + +  L+   +V++DDI+V+S +  EH   L  V E L    L  +  KCEF  ++ +FLGHV++ DG+  +P KIEA+  +
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSS-IEVRSFLGLAGYYRRFVENFSRIASPLTQLTRK
          P+   E+++FLGL GYYR+F+ NF+ IA P+T+  +K
Subjt:  SRPSS-IEVRSFLGLAGYYRRFVENFSRIASPLTQLTRK

P0CT41 Transposon Tf2-12 polyprotein

7.1e-22

31.85

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        M +G++ AP  F   +N +  +  ++ V+ ++DDIL++SK+E+EH  H++ VL+ L+   L    +KCEF   QV F+G+ +S+ G       I+ V  W
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSS-IEVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK
         +P +  E+R FLG   Y R+F+   S++  PL  L +K   + W+     + + +K
Subjt:  SRPSS-IEVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK

P10401 Retrovirus-related Pol polyprotein from transposon gypsy

3.8e-23

32.74

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        + FGL NA ++F   ++ V ++ +     V++DD++++S+ E++H  H+  VL+ L    +     K  F+   V +LG +VSKDG   DP K++A+  +
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTR-----------KGTPFVWSEACEDSFQILK
          P  + +VRSFLGLA YYR F+++F+ IA P+T + +           K  P  ++E   ++FQ L+
Subjt:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTR-----------KGTPFVWSEACEDSFQILK

P20825 Retrovirus-related Pol polyprotein from transposon 297

2.1e-26

39.01

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        M FGL NAP  F   MN + +  L+   +V++DDI+++S +  EH N ++ V   L    L  +  KCEF  ++ +FLGH+V+ DG+  +P K++A+ ++
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPS-SIEVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGT
          P+   E+R+FLGL GYYR+F+ N++ IA P+T   +K T
Subjt:  SRPS-SIEVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGT

Q8I7P9 Retrovirus-related Pol polyprotein from transposon opus

4.4e-24

37.68

Show/hide

Query:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW
        + FGL NAP +F  +++ + ++ +     V+IDDI+V+S+    H  +LR VL +L    L     K  F   QV FLG++V+ DG+  DP K+ A++  
Subjt:  MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNW

Query:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTR
          P+S+ E++ FLG+  YYR+F+++++++A PLT LTR
Subjt:  SRPSSI-EVRSFLGLAGYYRRFVENFSRIASPLTQLTR

Arabidopsis top hits

e value

%identity

Alignment

ATMG00860.1 DNA/RNA polymerases superfamily protein

1.9e-22

44.25

Show/hide

Query:  NHLRKVLETLRANQLFAKFSKCEFWLRQVSFLG--HVVSKDGVLVDPAKIEAVTNWSRP-SSIEVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFV
        NHL  VL+    +Q +A   KC F   Q+++LG  H++S +GV  DPAK+EA+  W  P ++ E+R FLGL GYYRRFV+N+ +I  PLT+L +K +   
Subjt:  NHLRKVLETLRANQLFAKFSKCEFWLRQVSFLG--HVVSKDGVLVDPAKIEAVTNWSRP-SSIEVRSFLGLAGYYRRFVENFSRIASPLTQLTRKGTPFV

Query:  WSEACEDSFQILK
        W+E    +F+ LK
Subjt:  WSEACEDSFQILK

Sequences

Show/hide sequences

CDS sequence

Show/hide CDS sequence

ATGTCATTTGGCTTGACTAACGCTCCTACAGTATTCATGGATCTGATGAATAGGGTGTTCAAGGATTTCTTGGACACTTTTGTAATTGTGTTCATTGATGACATCCTGGT
TTACTCCAAAACGGAGGCCGAGCATGAGAATCATTTGCGCAAGGTCTTGGAGACTCTTCGAGCTAATCAGTTGTTTGCCAAGTTCTCGAAGTGTGAATTCTGGTTGAGGC
AGGTATCTTTCTTAGGCCATGTGGTGTCCAAGGATGGTGTCTTAGTGGATCCAGCGAAGATAGAAGCAGTCACCAATTGGTCCAGACCCTCCTCAATCGAGGTTCGTAGT
TTTCTAGGTCTTGCAGGTTATTACCGCAGGTTCGTGGAGAACTTCTCCCGTATTGCTTCTCCTTTGACTCAGTTGACCAGGAAAGGGACTCCATTTGTTTGGAGTGAGGC
TTGTGAGGACAGTTTCCAAATTCTCAAGTAG

mRNA sequence

Show/hide mRNA sequence

Protein sequence

Show/hide protein sequence

MSFGLTNAPTVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHENHLRKVLETLRANQLFAKFSKCEFWLRQVSFLGHVVSKDGVLVDPAKIEAVTNWSRPSSIEVRS
FLGLAGYYRRFVENFSRIASPLTQLTRKGTPFVWSEACEDSFQILK

PI0015487 (gene) of Melon (PI 482460) v1 genome