CuGenDBv2

Gene ID	PI0015536
Organism	Cucumis metuliferus PI 482460 (Melon (PI 482460) v1)
Description	Prolyl-tRNA synthetase
Genome location	chr05:6793484..6795644
RNA-Seq Expression	PI0015536
Synteny	PI0015536
Gene Ontology terms	GO:0006433 - prolyl-tRNA aminoacylation (biological process) GO:0005737 - cytoplasm (cellular component) GO:0017101 - aminoacyl-tRNA synthetase multienzyme complex (cellular component) GO:0004827 - proline-tRNA ligase activity (molecular function) GO:0005524 - ATP binding (molecular function)
InterPro domains	IPR004499 - Proline-tRNA ligase, class IIa, archaeal-type IPR016061 - Proline-tRNA ligase, class II, C-terminal IPR017449 - Prolyl-tRNA synthetase, class II

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0050272.1 proline--tRNA ligase [Cucumis melo var. makuwa]

1.0e-45

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Query:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA
        PK L      VRAVRRDNSA KDIPR  LVEQVKELLESIQQSLFDAAKEK+DACIQV+NTWEEFTEALGQKKMILAPWC+EEEVEKDVKTRTKGE+GAA
Subjt:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA

Query:  KTLCSPFEQPPLPE-VVCFS
        KTLCSPFEQPPLPE   CF+
Subjt:  KTLCSPFEQPPLPE-VVCFS

XP_008466310.1 PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo]

1.0e-45

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Query:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA
        PK L      VRAVRRDNSA KDIPR  LVEQVKELLESIQQSLFDAAKEK+DACIQV+NTWEEFTEALGQKKMILAPWC+EEEVEKDVKTRTKGE+GAA
Subjt:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA

Query:  KTLCSPFEQPPLPE-VVCFS
        KTLCSPFEQPPLPE   CF+
Subjt:  KTLCSPFEQPPLPE-VVCFS

XP_008466343.1 PREDICTED: proline--tRNA ligase, cytoplasmic isoform X4 [Cucumis melo]

1.0e-45

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Query:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA
        PK L      VRAVRRDNSA KDIPR  LVEQVKELLESIQQSLFDAAKEK+DACIQV+NTWEEFTEALGQKKMILAPWC+EEEVEKDVKTRTKGE+GAA
Subjt:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA

Query:  KTLCSPFEQPPLPE-VVCFS
        KTLCSPFEQPPLPE   CF+
Subjt:  KTLCSPFEQPPLPE-VVCFS

XP_038907011.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida]

4.6e-46

85.83

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Query:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA
        PK L      VRAVRRDNS  KDIPRDSLVEQVKELLESIQQSLFDAAKEK+D CIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGE+GAA
Subjt:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA

Query:  KTLCSPFEQPPLPE-VVCFS
        KTLCSPFEQP LPE   CF+
Subjt:  KTLCSPFEQPPLPE-VVCFS

XP_038907039.1 proline--tRNA ligase, cytoplasmic-like isoform X2 [Benincasa hispida]

4.6e-46

85.83

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Query:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA
        PK L      VRAVRRDNS  KDIPRDSLVEQVKELLESIQQSLFDAAKEK+D CIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGE+GAA
Subjt:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA

Query:  KTLCSPFEQPPLPE-VVCFS
        KTLCSPFEQP LPE   CF+
Subjt:  KTLCSPFEQPPLPE-VVCFS

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KTZ1 Prolyl-tRNA synthetase

2.5e-45

84.17

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Query:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA
        PK L      VRAVRRDNS  KDIPRDSLVEQVKELLESIQQSLFDAAK K+D CIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGE+GAA
Subjt:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA

Query:  KTLCSPFEQPPLPE-VVCFS
        KTLCSPF+QP LPE   CF+
Subjt:  KTLCSPFEQPPLPE-VVCFS

A0A1S3CR78 Prolyl-tRNA synthetase

5.0e-46

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Query:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA
        PK L      VRAVRRDNSA KDIPR  LVEQVKELLESIQQSLFDAAKEK+DACIQV+NTWEEFTEALGQKKMILAPWC+EEEVEKDVKTRTKGE+GAA
Subjt:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA

Query:  KTLCSPFEQPPLPE-VVCFS
        KTLCSPFEQPPLPE   CF+
Subjt:  KTLCSPFEQPPLPE-VVCFS

A0A1S4DS87 Prolyl-tRNA synthetase

5.0e-46

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Query:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA
        PK L      VRAVRRDNSA KDIPR  LVEQVKELLESIQQSLFDAAKEK+DACIQV+NTWEEFTEALGQKKMILAPWC+EEEVEKDVKTRTKGE+GAA
Subjt:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA

Query:  KTLCSPFEQPPLPE-VVCFS
        KTLCSPFEQPPLPE   CF+
Subjt:  KTLCSPFEQPPLPE-VVCFS

A0A5A7TN35 Prolyl-tRNA synthetase

4.2e-45

84.17

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Query:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA
        PK L      VRAVRRDNSA KDIPRDSLVEQVKELLESIQQSLFDAAK K+D CIQVINTWEEFTEAL QKKMILAPWCDEEEVEKDVKTRTKGE+GAA
Subjt:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA

Query:  KTLCSPFEQPPLPE-VVCFS
        KTLCSPF+QP LPE   CF+
Subjt:  KTLCSPFEQPPLPE-VVCFS

A0A5A7U355 Prolyl-tRNA synthetase

5.0e-46

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Query:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA
        PK L      VRAVRRDNSA KDIPR  LVEQVKELLESIQQSLFDAAKEK+DACIQV+NTWEEFTEALGQKKMILAPWC+EEEVEKDVKTRTKGE+GAA
Subjt:  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAA

Query:  KTLCSPFEQPPLPE-VVCFS
        KTLCSPFEQPPLPE   CF+
Subjt:  KTLCSPFEQPPLPE-VVCFS

SwissProt top hits

e value

%identity

Alignment

O60155 Putative proline--tRNA ligase C19C7.06

1.6e-12

34.82

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Query:  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVG------------
        V AVRRD   K  +P ++L + V +LL  IQ ++++ AK ++DA +  +  W +F  AL +K +++ PWC+  E EK++K  +  +V             
Subjt:  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVG------------

Query:  AAKTLCSPFEQP
         AK+LC P EQP
Subjt:  AAKTLCSPFEQP

O82567 Choline/ethanolaminephosphotransferase 1

2.1e-25

82.54

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Query:  LYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTGMCM
        LYPFVVL+GGVL WD+LSP ++I +YPHLV+ GTGLAFGFLVGRMILAHLCDEPKGLKT MCM
Subjt:  LYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTGMCM

O82568 Choline/ethanolaminephosphotransferase 2

4.0e-24

79.37

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Query:  LYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTGMCM
        LYP V L+ GVL WD+LSP D+I +YPHLV+ GTGLAFGFLVGRMILAHLCDEPKGLKT MCM
Subjt:  LYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTGMCM

Q8I5R7 Proline--tRNA ligase

2.0e-15

37.69

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Query:  PKGLKTGMCMVRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCD----EEEVEKDVK----TR
        PK L+   C++  VRRDN+ K ++ ++S++ + +++L  I ++LF  AK+K D  I  + ++ E   AL +KKM+LAPWC+    EEE++K+ +     +
Subjt:  PKGLKTGMCMVRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCD----EEEVEKDVK----TR

Query:  TKGEV---GAAKTLCSPFEQPPL-PEVVCF
        T  E    GA K LC P +QPP+ P + CF
Subjt:  TKGEV---GAAKTLCSPFEQPPL-PEVVCF

Q9M1R2 Proline--tRNA ligase, cytoplasmic

2.8e-38

69.09

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Query:  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQP
        VR VRRDN  K DIPR SLVE VKELLE IQQ++++ AK+K++AC+Q + TW+EF +AL +KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP
Subjt:  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQP

Query:  PLPE-VVCFS
         LPE  +CF+
Subjt:  PLPE-VVCFS

Arabidopsis top hits

e value

%identity

Alignment

AT1G13560.1 aminoalcoholphosphotransferase 1

1.5e-26

82.54

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Query:  LYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTGMCM
        LYPFVVL+GGVL WD+LSP ++I +YPHLV+ GTGLAFGFLVGRMILAHLCDEPKGLKT MCM
Subjt:  LYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTGMCM

AT1G13560.2 aminoalcoholphosphotransferase 1

1.5e-26

82.54

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Query:  LYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTGMCM
        LYPFVVL+GGVL WD+LSP ++I +YPHLV+ GTGLAFGFLVGRMILAHLCDEPKGLKT MCM
Subjt:  LYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTGMCM

AT3G62120.1 Class II aaRS and biotin synthetases superfamily protein

2.0e-39

69.09

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Query:  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQP
        VR VRRDN  K DIPR SLVE VKELLE IQQ++++ AK+K++AC+Q + TW+EF +AL +KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP
Subjt:  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQP

Query:  PLPE-VVCFS
         LPE  +CF+
Subjt:  PLPE-VVCFS

AT3G62120.2 Class II aaRS and biotin synthetases superfamily protein

2.0e-39

69.09

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Query:  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQP
        VR VRRDN  K DIPR SLVE VKELLE IQQ++++ AK+K++AC+Q + TW+EF +AL +KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP
Subjt:  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQP

Query:  PLPE-VVCFS
         LPE  +CF+
Subjt:  PLPE-VVCFS

AT5G10880.1 tRNA synthetase-related / tRNA ligase-related

3.4e-31

59.13

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Query:  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEV-----GAAKTLCS
        VR V RDN AK D+ R  L+EQVK+LLE IQ +L+D AK K + C Q + TW+EF EAL QKK+ILAPWCD+ EVEKDVK RT+G+      G AKTLC+
Subjt:  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEV-----GAAKTLCS

Query:  PFEQPPL-PEVVCFS
        P EQP L  E +CF+
Subjt:  PFEQPPL-PEVVCFS

Sequences

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CDS sequence

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ATGCTTCTGTGCAGTGTGTACAATGTTTACAAGCTTTACCCGTTTGTAGTGCTTGTCGGAGGAGTCTTAGCATGGGATCATTTGTCACCTTCTGACATTATTGGAAGCTA
TCCCCATTTGGTTATAACTGGAACTGGACTTGCTTTTGGATTCTTAGTGGGTAGAATGATTCTAGCCCACCTTTGTGATGAACCCAAGGGCTTGAAAACTGGAATGTGCA
TGGTACGTGCAGTTCGGCGTGATAATTCTGCAAAGGACATACCTAGGGATTCGTTGGTTGAACAAGTGAAAGAGTTGCTAGAAAGTATTCAACAAAGCCTTTTTGATGCG
GCAAAAGAAAAACAAGATGCATGCATTCAGGTTATTAATACATGGGAGGAGTTTACTGAAGCCCTTGGTCAGAAGAAAATGATATTAGCTCCATGGTGTGATGAAGAGGA
GGTCGAGAAGGATGTGAAAACAAGGACTAAAGGTGAGGTGGGAGCAGCGAAAACCTTATGTTCCCCGTTCGAGCAGCCTCCCCTTCCAGAAGTTGTTTGTTTTTCATAG

mRNA sequence

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Protein sequence

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MLLCSVYNVYKLYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTGMCMVRAVRRDNSAKDIPRDSLVEQVKELLESIQQSLFDA
AKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEVVCFS

PI0015536 (gene) of Melon (PI 482460) v1 genome