CuGenDBv2

Gene ID	PI0016041
Organism	Cucumis metuliferus PI 482460 (Melon (PI 482460) v1)
Description	Hyoscyamine 6-dioxygenase-like
Genome location	chr01:9366866..9367869
RNA-Seq Expression	PI0016041
Synteny	PI0016041
Gene Ontology terms	GO:0016491 - oxidoreductase activity (molecular function)
InterPro domains	IPR027443 - Isopenicillin N synthase-like superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0059649.1 hyoscyamine 6-dioxygenase-like [Cucumis melo var. makuwa]

3.8e-26

64.76

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Query:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK
        MSQTKFVSNW+GVESVPETYVYPPEKRF +SVKKTIPVIDWA        KK        D +R    + VINHGV KTLVEETM++ KEFHAMSPE K+
Subjt:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK

Query:  GNAQK
            K
Subjt:  GNAQK

XP_004148957.1 hyoscyamine 6-dioxygenase [Cucumis sativus]

2.2e-21

57.14

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Query:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK
        MS+TKFVSNW GV SVPETYVYPPEKRF +SVKK+IPVIDW       S  +  +  +  D ++    + VINHGV KTLVEE M+I KEFH MS E K+
Subjt:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK

Query:  GNAQK
            K
Subjt:  GNAQK

XP_008451257.1 PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo]

5.0e-26

65.71

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Query:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK
        MSQTKFVSNW+GVESVPETYVYPPEKRF VSVKKTIPVIDWA        KK     +   F      + VINHGV KTLVEETM+I KEFHAMSPE K+
Subjt:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK

Query:  GNAQK
            K
Subjt:  GNAQK

XP_011659260.2 hyoscyamine 6-dioxygenase [Cucumis sativus]

2.3e-18

51.43

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Query:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK
        M+Q  FVSNWEGVESVPE+YV+P EKR  +++KKTIPV+D+A      +  +  +  K LD +    +  VINHGVSK LVEETM + KEFH+M P+ K+
Subjt:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK

Query:  GNAQK
            K
Subjt:  GNAQK

XP_011659768.2 protein DOWNY MILDEW RESISTANCE 6 [Cucumis sativus]

3.4e-22

60.95

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Query:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK
        MSQTKFVSNW+G+ SVPETYV PPEKR  VSVKK IPVID      L +  +  +S K LD S+      VINHGVSK LVEETMR+S+EFHAMS E K+
Subjt:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK

Query:  GNAQK
            K
Subjt:  GNAQK

TrEMBL top hits

e value

%identity

Alignment

A0A0A0K4U9 Fe2OG dioxygenase domain-containing protein

1.1e-18

51.43

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Query:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK
        M+Q  FVSNWEGVESVPE+YV+P EKR  +++KKTIPV+D+A      +  +  +  K LD +    +  VINHGVSK LVEETM + KEFH+M P+ K+
Subjt:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK

Query:  GNAQK
            K
Subjt:  GNAQK

A0A0A0K5H0 Fe2OG dioxygenase domain-containing protein

1.1e-21

57.14

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Query:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK
        MS+TKFVSNW GV SVPETYVYPPEKRF +SVKK+IPVIDW       S  +  +  +  D ++    + VINHGV KTLVEE M+I KEFH MS E K+
Subjt:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK

Query:  GNAQK
            K
Subjt:  GNAQK

A0A0A0K745 Fe2OG dioxygenase domain-containing protein

1.6e-22

60.95

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Query:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK
        MSQTKFVSNW+G+ SVPETYV PPEKR  VSVKK IPVID      L +  +  +S K LD S+      VINHGVSK LVEETMR+S+EFHAMS E K+
Subjt:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK

Query:  GNAQK
            K
Subjt:  GNAQK

A0A1S3BRU2 hyoscyamine 6-dioxygenase-like

2.4e-26

65.71

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Query:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK
        MSQTKFVSNW+GVESVPETYVYPPEKRF VSVKKTIPVIDWA        KK     +   F      + VINHGV KTLVEETM+I KEFHAMSPE K+
Subjt:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK

Query:  GNAQK
            K
Subjt:  GNAQK

A0A5A7V1J0 Hyoscyamine 6-dioxygenase-like

1.9e-26

64.76

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Query:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK
        MSQTKFVSNW+GVESVPETYVYPPEKRF +SVKKTIPVIDWA        KK        D +R    + VINHGV KTLVEETM++ KEFHAMSPE K+
Subjt:  MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKK

Query:  GNAQK
            K
Subjt:  GNAQK

SwissProt top hits

e value

%identity

Alignment

P24397 Hyoscyamine 6-dioxygenase

1.6e-06

36.36

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Query:  FVSNWEGVESVPETYVYPPEKRF--NVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETK----
        FVSNW   +SV E+++ P +KR   +V V   +P+ID  Q   L   +   ++    DF  F+    VINHG  + L+ ETM + KEF A+  E K    
Subjt:  FVSNWEGVESVPETYVYPPEKRF--NVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETK----

Query:  -KGNAQKIQI
         KG A K ++
Subjt:  -KGNAQKIQI

Arabidopsis top hits

e value

%identity

Alignment

AT5G20550.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein

1.2e-04

29.57

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Query:  MSQTKFVSNWEGV---ESVPETYVYPPE-----KRFNVSVK-KTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEF
        M + KF +  E V   E +PE Y+ PP      +  N +V    IP ID + ++      +  +S      S +  ++ VINHG++K L+++  +++KEF
Subjt:  MSQTKFVSNWEGV---ESVPETYVYPPE-----KRFNVSVK-KTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEF

Query:  HAMSPETKKGNAQKI
         A+  E K+  A++I
Subjt:  HAMSPETKKGNAQKI

AT5G24530.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein

3.4e-04

29.59

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Query:  KFVSNWEGVESVPETYVYPPEKR---FNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETK
        K +S      ++PE YV P   R     VS  +  P+ID +     F  ++   +     F        VINHGV+K +++E + +++EF +MS E K
Subjt:  KFVSNWEGVESVPETYVYPPEKR---FNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETK

Sequences

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CDS sequence

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ATGTCTCAAACGAAGTTTGTGTCTAATTGGGAGGGTGTTGAATCAGTTCCAGAAACTTATGTGTATCCTCCAGAGAAGAGGTTTAACGTCTCGGTGAAGAAGACGATTCC
AGTGATCGATTGGGCGCAGATGATCGAGCTGTTCTCCCCAAAAAAATTCTCGATGTCTCTCAAGAGTTTGGATTTTTCCAGGTTCGAAAGGCTAATGAATGTAATAAACC
ACGGCGTCTCAAAAACATTAGTAGAAGAGACAATGAGGATCTCGAAGGAGTTTCATGCCATGTCACCAGAGACAAAGAAAGGGAATGCTCAAAAGATCCAAATAAAAGTT
GTAGAATTTATTCAAGCTCTCCAAATTATGAAAAAGAACAAGTTCACCTCTGGAAAGATTCTTTGA

mRNA sequence

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Protein sequence

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MSQTKFVSNWEGVESVPETYVYPPEKRFNVSVKKTIPVIDWAQMIELFSPKKFSMSLKSLDFSRFERLMNVINHGVSKTLVEETMRISKEFHAMSPETKKGNAQKIQIKV
VEFIQALQIMKKNKFTSGKIL

PI0016041 (gene) of Melon (PI 482460) v1 genome