CuGenDBv2

Gene ID	PI0019720
Organism	Cucumis metuliferus PI 482460 (Melon (PI 482460) v1)
Description	PseudoU_synth_1 domain-containing protein
Genome location	chr12:24610498..24613418
RNA-Seq Expression	PI0019720
Synteny	PI0019720
Gene Ontology terms	GO:0031119 - tRNA pseudouridine synthesis (biological process) GO:0003723 - RNA binding (molecular function) GO:0009982 - pseudouridine synthase activity (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

XP_004145230.1 putative tRNA pseudouridine synthase isoform X2 [Cucumis sativus]

1.1e-32

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Query:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRGPHFMLLSVSLDHP
        M+VSSL LPLS WITK SSSCFRFP PVF SRTP LRP LL+ FSS TASTDAFST+CAPDQL KWQAFRKKKVVLRIGYVGTDYRG    +    L HP
Subjt:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRGPHFMLLSVSLDHP

XP_008457308.1 PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo]

1.6e-36

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Query:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRGPHFMLLSVSLDHP
        M+VSSL LPLS WITK+SSSCFRFPYPVF  RTPRLRPTLLYCFSS  ASTDAFSTQCAPDQL KWQAFRKKKVVLRIGYVGTDYRG    +    L HP
Subjt:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRGPHFMLLSVSLDHP

XP_031742713.1 putative tRNA pseudouridine synthase isoform X1 [Cucumis sativus]

9.9e-34

69.83

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Query:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRGPHFMLLSVSLDHP
        M+VSSL LPLS WITK SSSCFRFP PVF SRTP LRP LL+ FSS TASTDAFST+CAPDQL KWQAFRKKKVVLRIGYVGTDYRG    +    L HP
Subjt:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRGPHFMLLSVSLDHP

Query:  VANPSPFLINSLVLKI
          N +  L ++  LK+
Subjt:  VANPSPFLINSLVLKI

XP_038894776.1 putative tRNA pseudouridine synthase isoform X3 [Benincasa hispida]

4.6e-31

82.76

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Query:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRG
        MAVSSL LPLS WITKTSSSCF FPYP F SRT RL PTL  C +S TASTDAFSTQCAP+QL KW+AFRKKKVVLRIGYVGTDYRG
Subjt:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRG

XP_038894779.1 putative tRNA pseudouridine synthase isoform X5 [Benincasa hispida]

4.6e-31

82.76

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Query:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRG
        MAVSSL LPLS WITKTSSSCF FPYP F SRT RL PTL  C +S TASTDAFSTQCAP+QL KW+AFRKKKVVLRIGYVGTDYRG
Subjt:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRG

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LVB6 Uncharacterized protein

2.8e-58

78.67

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Query:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRGPHFMLLSVSLDHP
        M+VSSL LPLS WITK SSSCFRFP PVF SRTP LRP LL+ FSS TASTDAFST+CAPDQL KWQAFRKKKVVLRIGYVGTDYRGPHF LLSVSLDHP
Subjt:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRGPHFMLLSVSLDHP

Query:  VANPSPFLINSLVLKIQGYKCNATNCHIQQSNKNWKLQYIKLVEFVKVTM
        + NPSPFLINSLVL IQGYKCNATNCHIQ S   + L YI+ +    +TM
Subjt:  VANPSPFLINSLVLKIQGYKCNATNCHIQQSNKNWKLQYIKLVEFVKVTM

A0A1S3C5W1 putative tRNA pseudouridine synthase

7.9e-37

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Query:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRGPHFMLLSVSLDHP
        M+VSSL LPLS WITK+SSSCFRFPYPVF  RTPRLRPTLLYCFSS  ASTDAFSTQCAPDQL KWQAFRKKKVVLRIGYVGTDYRG    +    L HP
Subjt:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRGPHFMLLSVSLDHP

A0A6J1DMV6 putative tRNA pseudouridine synthase isoform X2

3.3e-27

74.71

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Query:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRG
        MAVSSL  PLS W TKTSS+CFR PY  F SR+P LRP LL C SSP+ASTDA ST+CAPDQL  W+AFRKKKVV+RIGY GTDYRG
Subjt:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRG

A0A6J1HM83 putative tRNA pseudouridine synthase

2.3e-28

77.01

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Query:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRG
        MAVSSL  PLS W  KTS  CFRFPYP F S T RL P LL C SS TASTDA STQCAPDQL KW+AFRKKKVV+RIGYVGTDYRG
Subjt:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRG

A0A6J1I156 putative tRNA pseudouridine synthase

1.2e-29

79.31

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Query:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRG
        MAVSSL  PLS W TKTS  CFRFPYP F S T RLRP LL C SS TASTDA STQCAPDQL KW+AFRKKKVV+RIGYVGTDYRG
Subjt:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRG

SwissProt top hits

e value

%identity

Alignment

O22928 Putative tRNA pseudouridine synthase

3.7e-07

42.86

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Query:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAP---DQLL--KWQAFRKKKVVLRIGYVGTDYRGPHFMLLSV
        MAVS L +P+ + +         F YP   +R  R+ P LL  FSS +A+    ST  +P   D  L  KW+++RKKKVV+RIGYVGTDYRG     L +
Subjt:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAP---DQLL--KWQAFRKKKVVLRIGYVGTDYRGPHFMLLSV

Query:  SLDHP
          D P
Subjt:  SLDHP

Arabidopsis top hits

e value

%identity

Alignment

AT2G30320.1 Pseudouridine synthase family protein

2.6e-08

42.86

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Query:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAP---DQLL--KWQAFRKKKVVLRIGYVGTDYRGPHFMLLSV
        MAVS L +P+ + +         F YP   +R  R+ P LL  FSS +A+    ST  +P   D  L  KW+++RKKKVV+RIGYVGTDYRG     L +
Subjt:  MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAP---DQLL--KWQAFRKKKVVLRIGYVGTDYRGPHFMLLSV

Query:  SLDHP
          D P
Subjt:  SLDHP

Sequences

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CDS sequence

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ATGGCAGTCTCTTCTTTGGGACTCCCACTTTCCACATGGATAACCAAAACTTCATCTTCATGTTTTCGTTTCCCCTACCCTGTTTTCGCTTCCCGGACCCCTCGTCTTAG
GCCTACACTGCTCTACTGTTTCTCATCTCCTACTGCCTCCACCGATGCTTTTTCCACTCAGTGCGCTCCGGACCAGCTCCTGAAATGGCAAGCTTTCCGTAAGAAGAAGG
TCGTTTTGCGAATCGGCTATGTCGGGACTGATTATCGAGGTCCTCATTTCATGTTACTCTCTGTTTCTCTTGACCACCCTGTTGCGAACCCTTCACCATTTCTCATTAAC
TCACTAGTTTTGAAAATTCAGGGCTACAAATGCAACGCAACCAACTGTCACATCCAACAATCGAACAAGAATTGGAAGCTGCAATATATAAAGCTGGTGGAATTCGTGAA
AGTAACTATGGAAATCTGA

mRNA sequence

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Protein sequence

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MAVSSLGLPLSTWITKTSSSCFRFPYPVFASRTPRLRPTLLYCFSSPTASTDAFSTQCAPDQLLKWQAFRKKKVVLRIGYVGTDYRGPHFMLLSVSLDHPVANPSPFLIN
SLVLKIQGYKCNATNCHIQQSNKNWKLQYIKLVEFVKVTMEI

PI0019720 (gene) of Melon (PI 482460) v1 genome