CuGenDBv2

Gene ID	PI0020352
Organism	Cucumis metuliferus PI 482460 (Melon (PI 482460) v1)
Description	Reverse transcriptase
Genome location	chr05:16984947..16985420
RNA-Seq Expression	PI0020352
Synteny	PI0020352
Gene Ontology terms	GO:0006278 - RNA-dependent DNA biosynthetic process (biological process) GO:0006355 - regulation of transcription, DNA-templated (biological process) GO:0006508 - proteolysis (biological process) GO:0015074 - DNA integration (biological process) GO:0090305 - nucleic acid phosphodiester bond hydrolysis (biological process) GO:0003700 - DNA-binding transcription factor activity (molecular function) GO:0003964 - RNA-directed DNA polymerase activity (molecular function) GO:0004190 - aspartic-type endopeptidase activity (molecular function) GO:0004519 - endonuclease activity (molecular function) GO:0008270 - zinc ion binding (molecular function) GO:0043565 - sequence-specific DNA binding (molecular function)
InterPro domains	IPR000477 - Reverse transcriptase domain IPR043128 - Reverse transcriptase/Diguanylate cyclase domain IPR043502 - DNA/RNA polymerase superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

AAO45752.1 pol protein [Cucumis melo subsp. melo]

8.0e-71

84.71

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Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVF+EFLDTFVIVFIDDIL+YSK E EHE+HLR VL+TL++N+L+AKFSKCEFWL+QVSFLGHVVSK  VSVDPAKIEAVT W
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK
        + PS++SEVRSFLGLAGYYRRFVE+FSRIA+PLTQLTRKG PFVW+KACEDSFQ LK
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK

KAA0040188.1 pol protein [Cucumis melo var. makuwa]

2.7e-71

85.99

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Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDIL+YSKME EHE+HLR VL+TL++N+L+AKFSKCEFWL+QVSFLGHVVSK  VSVDPAKIEAVT W
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK
        + PS++SEVRSFLGLAGYYRRFVE+FSRIA+PLTQLTRKG PFVW+KACEDSFQ LK
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK

KAA0046014.1 gag protease polyprotein [Cucumis melo var. makuwa]

9.4e-72

85.35

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Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVF+EFLDTFVIVFIDDIL+YSK E EHE+HLR VL+TL++N+L+AKFSKCEFWL+QVSFLGHVVSKD VSVDPAKIEAVT W
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK
        + PS++SEVRSFLGLAGYYRRFVE+FSRIA+PLTQLTRKG PFVW+KACEDSFQ LK
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK

KAA0060847.1 pol protein [Cucumis melo var. makuwa]

9.4e-72

85.35

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVF+EFLDTFVIVFIDDIL+YSK E EHE+HLR VL+TL++N+L+AKFSKCEFWL+QVSFLGHVVSKD VSVDPAKIEAVT W
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK
        + PS++SEVRSFLGLAGYYRRFVE+FSRIA+PLTQLTRKG PFVW+KACEDSFQ LK
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK

KAA0066266.1 pol protein [Cucumis melo var. makuwa]

8.0e-71

84.71

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVF+EFLDTFVIVFIDDIL+YSK E EHE+HLR VL+TL++N+L+AKFSKCEFWL+QVSFLGHVVSK  VSVDPAKIEAVT W
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK
        + PS++SEVRSFLGLAGYYRRFVE+FSRIA+PLTQLTRKG PFVW+KACEDSFQ LK
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK

TrEMBL top hits

e value

%identity

Alignment

A0A5A7TB42 Reverse transcriptase

1.3e-71

85.99

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDIL+YSKME EHE+HLR VL+TL++N+L+AKFSKCEFWL+QVSFLGHVVSK  VSVDPAKIEAVT W
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK
        + PS++SEVRSFLGLAGYYRRFVE+FSRIA+PLTQLTRKG PFVW+KACEDSFQ LK
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK

A0A5A7TSI1 Gag protease polyprotein

4.6e-72

85.35

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVF+EFLDTFVIVFIDDIL+YSK E EHE+HLR VL+TL++N+L+AKFSKCEFWL+QVSFLGHVVSKD VSVDPAKIEAVT W
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK
        + PS++SEVRSFLGLAGYYRRFVE+FSRIA+PLTQLTRKG PFVW+KACEDSFQ LK
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK

A0A5A7UPE4 Ty3-gypsy retrotransposon protein

3.9e-71

85.35

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVF+EFLDTFVIVFIDDIL+YSK EVEHE+HLR VL+TL++N+L+AKFSKCEFWL+ VSFLGHVVSKD VSVDP KIEAVT+W
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK
        + PS+ISEVRSFLGLAGYYRRFVE+FSRIA+PLTQLTRKG PFVW+KACEDSFQ LK
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK

A0A5A7V1X7 Pol protein

4.6e-72

85.35

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVF+EFLDTFVIVFIDDIL+YSK E EHE+HLR VL+TL++N+L+AKFSKCEFWL+QVSFLGHVVSKD VSVDPAKIEAVT W
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK
        + PS++SEVRSFLGLAGYYRRFVE+FSRIA+PLTQLTRKG PFVW+KACEDSFQ LK
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK

A0A5A7VIT9 Reverse transcriptase

3.9e-71

84.71

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVF+EFLDTFVIVFIDDIL+YSK E EHE+HLR VL+TL++N+L+AKFSKCEFWL+QVSFLGHVVSK  VSVDPAKIEAVT W
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK
        + PS++SEVRSFLGLAGYYRRFVE+FSRIA+PLTQLTRKG PFVW+KACEDSFQ LK
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK

SwissProt top hits

e value

%identity

Alignment

P04323 Retrovirus-related Pol polyprotein from transposon 17.6

2.5e-27

38.61

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Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        M FGL NAPA F   MN + +  L+   +V++DDI+V+S    EH   L  V E L +  L  +  KCEF  ++ +FLGHV++ D +  +P KIEA+  +
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPF-VWNKACEDSFQVLK
         +P+   E+++FLGL GYYR+F+ +F+ IA P+T+  +K       N   + +F+ LK
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPF-VWNKACEDSFQVLK

P0CT41 Transposon Tf2-12 polyprotein

7.1e-22

32.48

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Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        M +G++ APA F   +N +  E  ++ V+ ++DDIL++SK E EH  H++ VL+ L+   L    +KCEF   QV F+G+ +S+   +     I+ V  W
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK
          P +  E+R FLG   Y R+F+   S++  PL  L +K   + W      + + +K
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK

P10401 Retrovirus-related Pol polyprotein from transposon gypsy

1.0e-23

33.33

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Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        + FGL NA ++F   ++ V +E +     V++DD++++S+ E +H  H+  VL+ L +  +     K  F+   V +LG +VSKD    DP K++A+  +
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTR-----------KGTPFVWNKACEDSFQVLK
          P  + +VRSFLGLA YYR F++DF+ IA P+T + +           K  P  +N+   ++FQ L+
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTR-----------KGTPFVWNKACEDSFQVLK

P20825 Retrovirus-related Pol polyprotein from transposon 297

5.6e-27

37.59

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Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        M FGL NAPA F   MN + +  L+   +V++DDI+++S    EH + ++ V   L +  L  +  KCEF  ++ +FLGH+V+ D +  +P K++A+ ++
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGT
         +P+   E+R+FLGL GYYR+F+ +++ IA P+T   +K T
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGT

Q8I7P9 Retrovirus-related Pol polyprotein from transposon opus

1.1e-25

39.13

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Query:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW
        + FGL NAPA+F  +++ + +E +     V+IDDI+V+S+    H  +LR VL +L +  L     K  F   QV FLG++V+ D +  DP K+ A++  
Subjt:  MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNW

Query:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTR
          P+S+ E++ FLG+  YYR+F++D++++A PLT LTR
Subjt:  SVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTR

Arabidopsis top hits

e value

%identity

Alignment

ATMG00860.1 DNA/RNA polymerases superfamily protein

4.3e-22

41.59

Show/hide

Query:  DHLRKVLETLQENQLFAKFSKCEFWLRQVSFLG--HVVSKDSVSVDPAKIEAVTNWSVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFV
        +HL  VL+  +++Q +A   KC F   Q+++LG  H++S + VS DPAK+EA+  W  P + +E+R FLGL GYYRRFV+++ +I  PLT+L +K +   
Subjt:  DHLRKVLETLQENQLFAKFSKCEFWLRQVSFLG--HVVSKDSVSVDPAKIEAVTNWSVPSSISEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFV

Query:  WNKACEDSFQVLK
        W +    +F+ LK
Subjt:  WNKACEDSFQVLK

Sequences

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CDS sequence

Show/hide CDS sequence

ATGTCTTTTGGTTTGACTAACGCTCCTGCTGTATTTATGGATCTGATGAACAGGGTGTTTAAGGAATTCCTGGACACCTTTGTGATTGTGTTCATTGATGACATCTTAGT
CTACTCCAAGATGGAGGTCGAGCATGAGGATCATTTGCGTAAGGTCTTGGAGACACTTCAAGAGAACCAGTTATTTGCTAAGTTCTCGAAGTGCGAATTTTGGTTGAGGC
AGGTATCTTTCCTAGGCCATGTGGTGTCCAAGGACAGTGTTTCAGTGGATCCAGCTAAGATTGAAGCGGTCACCAACTGGTCCGTACCTTCCTCAATTAGCGAGGTTCGT
AGTTTTCTGGGTCTTGCAGGTTATTATCGTAGGTTTGTGGAGGATTTCTCCCGCATTGCTTCTCCGTTGACCCAGTTGACCAGAAAAGGGACTCCATTTGTGTGGAATAA
GGCTTGTGAGGACAGTTTCCAGGTCCTCAAGTAG

mRNA sequence

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Protein sequence

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MSFGLTNAPAVFMDLMNRVFKEFLDTFVIVFIDDILVYSKMEVEHEDHLRKVLETLQENQLFAKFSKCEFWLRQVSFLGHVVSKDSVSVDPAKIEAVTNWSVPSSISEVR
SFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFVWNKACEDSFQVLK

PI0020352 (gene) of Melon (PI 482460) v1 genome