CuGenDBv2

Gene ID	PI0029162
Organism	Cucumis metuliferus PI 482460 (Melon (PI 482460) v1)
Description	Reverse transcriptase
Genome location	chr06:16251511..16251984
RNA-Seq Expression	PI0029162
Synteny	PI0029162
Gene Ontology terms	GO:0006278 - RNA-dependent DNA biosynthetic process (biological process) GO:0006508 - proteolysis (biological process) GO:0015074 - DNA integration (biological process) GO:0090305 - nucleic acid phosphodiester bond hydrolysis (biological process) GO:0003676 - nucleic acid binding (molecular function) GO:0003964 - RNA-directed DNA polymerase activity (molecular function) GO:0004190 - aspartic-type endopeptidase activity (molecular function) GO:0004519 - endonuclease activity (molecular function) GO:0008270 - zinc ion binding (molecular function)
InterPro domains	IPR000477 - Reverse transcriptase domain IPR043128 - Reverse transcriptase/Diguanylate cyclase domain IPR043502 - DNA/RNA polymerase superfamily

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAA0025998.1 pol protein [Cucumis melo var. makuwa]

2.2e-73

87.9

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVFKDFLD+FVIVFIDDIL+YSKTEAEHE HL +VLETLRAN+L  KFSKCEFWLR+V+FLGHVVS +GVSVDPAKIEAVTNW
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK
        +RPS+VSE+RSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPF+WS ACE SFQ LK
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK

KAA0033486.1 reverse transcriptase [Cucumis melo var. makuwa]

1.7e-73

87.9

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVFKDFLD+FVIVFIDDIL+YSKTEAEHE HL +VLETLRAN+L  KFSKCEFWLR+V+FLGHVVS++GVSVDPAKIEAVTNW
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK
         RPS+VSE+RSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPF+WS ACE SFQ LK
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK

KAA0046571.1 reverse transcriptase [Cucumis melo var. makuwa]

2.2e-73

87.9

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVFKDFLD+FVIVFIDDIL+YSKTEAEHE HL +VLETLRAN+L  KFSKCEFWLR+V+FLGHVVS +GVSVDPAKIEAVTNW
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK
        +RPS+VSE+RSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPF+WS ACE SFQ LK
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK

TYK01576.1 pol protein [Cucumis melo var. makuwa]

2.2e-73

87.9

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVFKDFLD+FVIVFIDDIL+YSKTEAEHE HL +VLETLRAN+L  KFSKCEFWLR+V+FLGHVVS +GVSVDPAKIEAVTNW
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK
        +RPS+VSE+RSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPF+WS ACE SFQ LK
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK

TYK20443.1 pol protein [Cucumis melo var. makuwa]

2.2e-73

87.9

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVFKDFLD+FVIVFIDDIL+YSKTEAEHE HL +VLETLRAN+L  KFSKCEFWLR+V+FLGHVVS +GVSVDPAKIEAVTNW
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK
        +RPS+VSE+RSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPF+WS ACE SFQ LK
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK

TrEMBL top hits

e value

%identity

Alignment

A0A5A7SU25 Reverse transcriptase

8.3e-74

87.9

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVFKDFLD+FVIVFIDDIL+YSKTEAEHE HL +VLETLRAN+L  KFSKCEFWLR+V+FLGHVVS++GVSVDPAKIEAVTNW
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK
         RPS+VSE+RSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPF+WS ACE SFQ LK
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK

A0A5A7UIB4 Pol protein

1.1e-73

87.9

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVFKDFLD+FVIVFIDDIL+YSKTEAEHE HL +VLETLRAN+L  KFSKCEFWLR+V+FLGHVVS +GVSVDPAKIEAVTNW
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK
        +RPS+VSE+RSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPF+WS ACE SFQ LK
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK

A0A5A7V4E4 Reverse transcriptase

1.1e-73

87.9

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVFKDFLD+FVIVFIDDIL+YSKTEAEHE HL +VLETLRAN+L  KFSKCEFWLR+V+FLGHVVS +GVSVDPAKIEAVTNW
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK
         RPS+VSE+RSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPF+WS ACE SFQ LK
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK

A0A5A7VMR4 Reverse transcriptase

1.1e-73

87.9

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVFKDFLD+FVIVFIDDIL+YSKTEAEHE HL +VLETLRAN+L  KFSKCEFWLR+V+FLGHVVS +GVSVDPAKIEAVTNW
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK
         RPS+VSE+RSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPF+WS ACE SFQ LK
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK

A0A5D3BTN0 Reverse transcriptase

1.1e-73

87.9

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        MSFGLTNAPAVFMDLMNRVFKDFLD+FVIVFIDDIL+YSKTEAEHE HL +VLETLRAN+L  KFSKCEFWLR+V+FLGHVVS +GVSVDPAKIEAVTNW
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK
        +RPS+VSE+RSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPF+WS ACE SFQ LK
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK

SwissProt top hits

e value

%identity

Alignment

P04323 Retrovirus-related Pol polyprotein from transposon 17.6

1.1e-27

41.73

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        M FGL NAPA F   MN + +  L+   +V++DDI+V+S +  EH   L  V E L    L  +  KCEF  ++ +FLGHV++ DG+  +P KIEA+  +
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRK
          P+   E+++FLGL GYYR+F+ +F+ IA P+T+  +K
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRK

P0CT41 Transposon Tf2-12 polyprotein

2.2e-23

32.48

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        M +G++ APA F   +N +  +  ++ V+ ++DDIL++SK+E+EH  H++ VL+ L+   L    +KCEF   QV F+G+ +S+ G +     I+ V  W
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK
         +P +  E+R FLG   Y R+F+   S++  PL  L +K   + W+     + + +K
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK

P10401 Retrovirus-related Pol polyprotein from transposon gypsy

3.1e-25

33.93

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        + FGL NA ++F   ++ V ++ +     V++DD++++S+ E++H  H+  VL+ L    +     K  F+   V +LG +VSKDG   DP K++A+  +
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTR-----------KGTPFIWSEACEDSFQILK
          P  V +VRSFLGLA YYR F++DF+ IA P+T + +           K  P  ++E   ++FQ L+
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTR-----------KGTPFIWSEACEDSFQILK

P20825 Retrovirus-related Pol polyprotein from transposon 297

1.1e-27

38.3

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        M FGL NAPA F   MN + +  L+   +V++DDI+++S +  EH   ++ V   L    L  +  KCEF  ++ +FLGH+V+ DG+  +P K++A+ ++
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGT
          P+   E+R+FLGL GYYR+F+ +++ IA P+T   +K T
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGT

Q8I7P9 Retrovirus-related Pol polyprotein from transposon opus

4.3e-27

39.86

Show/hide

Query:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW
        + FGL NAPA+F  +++ + ++ +     V+IDDI+V+S+    H  +LR VL +L    L     K  F   QV FLG++V+ DG+  DP K+ A++  
Subjt:  MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNW

Query:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTR
          P+SV E++ FLG+  YYR+F++D++++A PLT LTR
Subjt:  SRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTR

Arabidopsis top hits

e value

%identity

Alignment

ATMG00860.1 DNA/RNA polymerases superfamily protein

4.3e-22

42.86

Show/hide

Query:  HLRKVLETLRANQLSTKFSKCEFWLRQVSFLG--HVVSKDGVSVDPAKIEAVTNWSRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIW
        HL  VL+    +Q      KC F   Q+++LG  H++S +GVS DPAK+EA+  W  P + +E+R FLGL GYYRRFV+++ +I  PLT+L +K +   W
Subjt:  HLRKVLETLRANQLSTKFSKCEFWLRQVSFLG--HVVSKDGVSVDPAKIEAVTNWSRPSSVSEVRSFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIW

Query:  SEACEDSFQILK
        +E    +F+ LK
Subjt:  SEACEDSFQILK

Sequences

Show/hide sequences

CDS sequence

Show/hide CDS sequence

ATGTCATTTGGTTTGACTAATGCTCCTGCAGTATTTATGGATCTGATGAACAGGGTGTTCAAGGATTTCTTGGACACTTTTGTAATTGTGTTCATTGATGACATCTTGGT
TTACTCCAAGACGGAGGCCGAGCATGAGGGTCATTTGCGCAAGGTCTTGGAGACTCTTCGAGCTAATCAATTGTCTACTAAGTTCTCGAAGTGTGAATTCTGGTTGAGAC
AGGTATCTTTCCTAGGCCATGTGGTGTCCAAGGATGGTGTTTCGGTGGATCCAGCAAAGATTGAAGCGGTCACCAATTGGTCCAGACCTTCCTCAGTTAGCGAGGTTCGT
AGTTTTCTAGGTCTTGCAGGTTATTACCGCAGGTTTGTGGAGGACTTCTCCCGTATTGCTTCTCCTTTGACTCAATTGACTAGGAAGGGGACTCCATTTATTTGGAGTGA
GGCTTGTGAGGATAGTTTCCAAATTCTCAAGTAG

mRNA sequence

Show/hide mRNA sequence

Protein sequence

Show/hide protein sequence

MSFGLTNAPAVFMDLMNRVFKDFLDTFVIVFIDDILVYSKTEAEHEGHLRKVLETLRANQLSTKFSKCEFWLRQVSFLGHVVSKDGVSVDPAKIEAVTNWSRPSSVSEVR
SFLGLAGYYRRFVEDFSRIASPLTQLTRKGTPFIWSEACEDSFQILK

PI0029162 (gene) of Melon (PI 482460) v1 genome