CuGenDBv2

Gene ID	Sgr022059
Organism	Siraitia grosvenorii cv. Qingpiguo (Monk fruit (Qingpiguo) v1)
Description	(-)-germacrene D synthase-like isoform X2
Genome location	tig00153874:553609..553902
RNA-Seq Expression	Sgr022059
Synteny	Sgr022059
Gene Ontology terms	GO:0016102 - diterpenoid biosynthetic process (biological process) GO:0000287 - magnesium ion binding (molecular function) GO:0010333 - terpene synthase activity (molecular function)
InterPro domains	IPR001906 - Terpene synthase, N-terminal domain IPR008930 - Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid IPR036965 - Terpene synthase, N-terminal domain superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

XP_022143526.1 (-)-germacrene D synthase-like isoform X1 [Momordica charantia]

3.4e-19

77.14

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC
        M+ A +K SEKLSLI+SIQ LGLSYHFEGEINE LE+MQN   N+DDDEDIY  ALRFRLLRQQGY VSC
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC

XP_022143529.1 (-)-germacrene D synthase-like isoform X1 [Momordica charantia]

1.2e-19

78.57

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC
        ML A EK SEKLSLI+SIQ LG+SYHFEGEINE LE+MQN   N+DDDEDIY  ALRFRLLRQQGY VSC
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC

XP_022143530.1 valencene synthase-like isoform X2 [Momordica charantia]

1.2e-19

78.57

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC
        ML A EK SEKLSLI+SIQ LG+SYHFEGEINE LE+MQN   N+DDDEDIY  ALRFRLLRQQGY VSC
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC

XP_022143531.1 (-)-germacrene D synthase-like isoform X1 [Momordica charantia]

6.8e-20

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC
        ML A EK SEKLSLI+SIQ LGLSYHFEGEINE LE+MQN   N+DDDEDIY  ALRFRLLRQQGY VSC
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC

XP_022143532.1 (-)-germacrene D synthase-like isoform X2 [Momordica charantia]

6.8e-20

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC
        ML A EK SEKLSLI+SIQ LGLSYHFEGEINE LE+MQN   N+DDDEDIY  ALRFRLLRQQGY VSC
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC

TrEMBL top hits

e value

%identity

Alignment

A0A6J1CP21 valencene synthase-like isoform X2

5.6e-20

78.57

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC
        ML A EK SEKLSLI+SIQ LG+SYHFEGEINE LE+MQN   N+DDDEDIY  ALRFRLLRQQGY VSC
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC

A0A6J1CPK3 (-)-germacrene D synthase-like isoform X1

5.6e-20

78.57

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC
        ML A EK SEKLSLI+SIQ LG+SYHFEGEINE LE+MQN   N+DDDEDIY  ALRFRLLRQQGY VSC
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC

A0A6J1CQI4 (-)-germacrene D synthase-like isoform X1

1.6e-19

77.14

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC
        M+ A +K SEKLSLI+SIQ LGLSYHFEGEINE LE+MQN   N+DDDEDIY  ALRFRLLRQQGY VSC
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC

A0A6J1CQI9 (-)-germacrene D synthase-like isoform X1

3.3e-20

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC
        ML A EK SEKLSLI+SIQ LGLSYHFEGEINE LE+MQN   N+DDDEDIY  ALRFRLLRQQGY VSC
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC

A0A6J1CR35 (-)-germacrene D synthase-like isoform X2

3.3e-20

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC
        ML A EK SEKLSLI+SIQ LGLSYHFEGEINE LE+MQN   N+DDDEDIY  ALRFRLLRQQGY VSC
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC

SwissProt top hits

e value

%identity

Alignment

B2KSJ5 (+)-gamma-cadinene synthase

5.4e-12

49.35

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPHN--------VDDDEDIYFTALRFRLLRQQGYFVSC
        M+A VE    KL+L++SIQ LG+SYHFE E++E LE++   +N          + ED++ TAL FRLLRQQGY +SC
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPHN--------VDDDEDIYFTALRFRLLRQQGYFVSC

B9SBV6 Probable terpene synthase 3

4.4e-14

58.82

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPHNVDDDEDIYFTALRFRLLRQQGYFVS
        +++  +KPS KL LI+ IQ LG++YHFE EI E +E + +  N DDDED+Y  ALRFRLLRQQGY VS
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPHNVDDDEDIYFTALRFRLLRQQGYFVS

B9SCB6 Probable terpene synthase 2

2.4e-12

51.43

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC
        M+A+ ++PS+ L+LI+ +Q LG+SYHFE EI E L+ + N + + D D+D++  ALRFRLLR+QG  VSC
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPH-NVDDDEDIYFTALRFRLLRQQGYFVSC

Q6Q3H2 Valencene synthase

4.1e-12

53.62

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Query:  AAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNP-HNVDD-DEDIYFTALRFRLLRQQGYFVSC
        AAV  PS+ L+ I+++Q LG++YHFE EI E L+++ N  H+ +D D D+Y  AL FRLLRQQGY +SC
Subjt:  AAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNP-HNVDD-DEDIYFTALRFRLLRQQGYFVSC

Q6Q3H3 (-)-germacrene D synthase

5.8e-14

53.62

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Query:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPHNVDDDEDIYFTALRFRLLRQQGYFVSC
        ++AA +  ++KL LI++IQ LG++YHFE EI+E L++M +   V  +ED+Y  +LRFRLLRQQGY VSC
Subjt:  MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPHNVDDDEDIYFTALRFRLLRQQGYFVSC

Arabidopsis top hits

e value

%identity

Alignment

AT1G61680.2 terpene synthase 14

9.8e-09

39.33

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Query:  AAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPHNVDDDEDIYFTALRFRLLRQQGYFVSCGESLEQSSKNYF--AIENDLFG
        A V+ PSE L +I+ IQ LG+  HF  EI +TL +M     +  + D++  ALRFRLLRQ+G++V      +++ K  F   ++ND+ G
Subjt:  AAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPHNVDDDEDIYFTALRFRLLRQQGYFVSCGESLEQSSKNYF--AIENDLFG

AT3G25810.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein

4.7e-11

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Query:  MLAAVEKPS--EKLSLINSIQHLGLSYHFEGEINETLE--YMQNPHNV---DDDEDIYFTALRFRLLRQQGYFVS
        ML  VE  S  EKL LI+ +Q LG+SYHFE EIN  L   +++N  N+   D +ED++ TAL FRLLRQ G+ VS
Subjt:  MLAAVEKPS--EKLSLINSIQHLGLSYHFEGEINETLE--YMQNPHNV---DDDEDIYFTALRFRLLRQQGYFVS

AT3G25820.1 terpene synthase-like sequence-1,8-cineole

1.5e-09

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Query:  EKLSLINSIQHLGLSYHFEGEINETLEYM-----QNPHNVDDDEDIYFTALRFRLLRQQGYFVS
        E+L LI+ +Q LG+SYHFE EIN+TL  +     +N    D +ED++ T+L FRLLRQ G+ VS
Subjt:  EKLSLINSIQHLGLSYHFEGEINETLEYM-----QNPHNVDDDEDIYFTALRFRLLRQQGYFVS

AT3G25820.2 terpene synthase-like sequence-1,8-cineole

1.5e-09

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Query:  EKLSLINSIQHLGLSYHFEGEINETLEYM-----QNPHNVDDDEDIYFTALRFRLLRQQGYFVS
        E+L LI+ +Q LG+SYHFE EIN+TL  +     +N    D +ED++ T+L FRLLRQ G+ VS
Subjt:  EKLSLINSIQHLGLSYHFEGEINETLEYM-----QNPHNVDDDEDIYFTALRFRLLRQQGYFVS

AT3G25830.1 terpene synthase-like sequence-1,8-cineole

1.5e-09

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Query:  EKLSLINSIQHLGLSYHFEGEINETLEYM-----QNPHNVDDDEDIYFTALRFRLLRQQGYFVS
        E+L LI+ +Q LG+SYHFE EIN+TL  +     +N    D +ED++ T+L FRLLRQ G+ VS
Subjt:  EKLSLINSIQHLGLSYHFEGEINETLEYM-----QNPHNVDDDEDIYFTALRFRLLRQQGYFVS

Sequences

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CDS sequence

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ATGCTTGCAGCTGTTGAAAAACCTTCAGAAAAACTAAGTTTGATTAATTCAATCCAGCATCTAGGTCTGTCTTACCATTTTGAAGGGGAAATTAATGAAACTTTAGAGTA
CATGCAAAATCCTCACAATGTTGATGATGATGAGGATATTTACTTTACCGCTCTTAGGTTTCGATTGCTTAGACAACAAGGTTACTTTGTTTCATGTGGTGAGTCCCTCG
AGCAATCTTCAAAGAATTACTTCGCTATAGAAAATGATTTATTTGGGTACCCTTCAAACAGAGATATTCAATAA

mRNA sequence

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Protein sequence

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MLAAVEKPSEKLSLINSIQHLGLSYHFEGEINETLEYMQNPHNVDDDEDIYFTALRFRLLRQQGYFVSCGESLEQSSKNYFAIENDLFGYPSNRDIQ

Sgr022059 (gene) of Monk fruit (Qingpiguo) v1 genome