CuGenDBv2

Gene ID	Sgr022210
Organism	Siraitia grosvenorii cv. Qingpiguo (Monk fruit (Qingpiguo) v1)
Description	Threonylcarbamoyladenosine tRNA methylthiotransferase
Genome location	tig00153996:210498..211010
RNA-Seq Expression	Sgr022210
Synteny	Sgr022210
Gene Ontology terms	GO:0035600 - tRNA methylthiolation (biological process) GO:0005783 - endoplasmic reticulum (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0035598 - N6-threonylcarbomyladenosine methylthiotransferase activity (molecular function) GO:0046872 - metal ion binding (molecular function) GO:0051539 - 4 iron, 4 sulfur cluster binding (molecular function) GO:0061712 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase (molecular function)
InterPro domains	IPR003616 - Post-SET domain

Homology

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GenBank top hits

e value

%identity

Alignment

XP_022949069.1 threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita moschata]

3.8e-74

85.29

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Query:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPES-CKGPVTLDDKDDSSRNIPLPEEPNRKNLI
        MLGTSA+VKITSVGRWSVFGEVI+TI TKHHKTT LEDMPIQ KVPPCS+ HETCAC +EPE CACGPES CKGPV LD+KDDSSRNIPLPEEP RKNL+
Subjt:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPES-CKGPVTLDDKDDSSRNIPLPEEPNRKNLI

Query:  EWLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSN
        EWLLR+RKSH PKREE+ESP+ SERKQ SAGG M EWGVVDRILVGGILISIFTIVGLLFHL STTLSS+
Subjt:  EWLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSN

XP_022997808.1 threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita maxima]

3.8e-74

85.88

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Query:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPES-CKGPVTLDDKDDSSRNIPLPEEPNRKNLI
        MLGTSA+VKITSVGRWSVFGEVI+TI TKHHKTT LEDMPIQ KVPPCSN+HETCAC +EPE CACGPES CKGPV LD+KDDSSRNIPLPEEP RKNL+
Subjt:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPES-CKGPVTLDDKDDSSRNIPLPEEPNRKNLI

Query:  EWLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSN
        EWLLR+RKSHVPKREE ESP+ S RKQ SAGG M EWGVVDRILVGGILISIFTIVGLLFHL STTLSS+
Subjt:  EWLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSN

XP_023524910.1 threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita pepo subsp. pepo]

2.2e-74

85.88

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Query:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPES-CKGPVTLDDKDDSSRNIPLPEEPNRKNLI
        MLGTSA+VKITSVGRWSVFGEVI+TI TKHHKTT LEDMPIQ KVPPCSN HETCAC +EPE CACG ES CKGPV LD+KDDSSRNIPLPEEP RKNL+
Subjt:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPES-CKGPVTLDDKDDSSRNIPLPEEPNRKNLI

Query:  EWLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSN
        EWLLR+RKSHVPKREE+ESP+ SERKQ SAGG M EWGVVDRILVGGILISIFTIVGLLFHL STTLSS+
Subjt:  EWLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSN

XP_038890156.1 threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Benincasa hispida]

2.2e-74

84.12

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Query:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPESCKGPVTLDDKDDSSRNIPLPEEPNRKNLIE
        MLGTSAMVKITS GRWSVFGEVI+TISTKHHKTTTLED PI+DKVPPCSN HETCAC +EPE CACGPESCKG V  DDKDDSSRNIPLPEEP RKNL+E
Subjt:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPESCKGPVTLDDKDDSSRNIPLPEEPNRKNLIE

Query:  WLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSNW
        W LRRRKSHVPKREE+ESPI SERKQ  AGG + EWGV+DRILVGGILIS  TI GLLFHL STTLSS+W
Subjt:  WLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSNW

XP_038890157.1 threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2 [Benincasa hispida]

2.2e-74

84.12

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Query:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPESCKGPVTLDDKDDSSRNIPLPEEPNRKNLIE
        MLGTSAMVKITS GRWSVFGEVI+TISTKHHKTTTLED PI+DKVPPCSN HETCAC +EPE CACGPESCKG V  DDKDDSSRNIPLPEEP RKNL+E
Subjt:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPESCKGPVTLDDKDDSSRNIPLPEEPNRKNLIE

Query:  WLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSNW
        W LRRRKSHVPKREE+ESPI SERKQ  AGG + EWGV+DRILVGGILIS  TI GLLFHL STTLSS+W
Subjt:  WLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSNW

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KSZ4 Threonylcarbamoyladenosine tRNA methylthiotransferase

3.4e-65

77.19

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Query:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPESCKGPVTLDDKDDSSRNIPLPEEPNRKNLIE
        MLGTSA VKITS+GRWSVFGEVI+ ISTKHHKTTTLED   QDKV PCSNTHETCAC  EPE CACG ESCKG V + D+ +SSRN+P PEEP RKNLIE
Subjt:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPESCKGPVTLDDKDDSSRNIPLPEEPNRKNLIE

Query:  WLLRRRKSHV-PKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSNW
        W+LRRRKSHV PKREE E+PI +ERKQ  AGG + EWGVVD+ILVGGILIS FTI GLLFHL STT SS+W
Subjt:  WLLRRRKSHV-PKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSNW

A0A1S3CA89 Threonylcarbamoyladenosine tRNA methylthiotransferase

2.7e-62

76.61

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Query:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPESCKGPVTLDDKDDSSRNIPLPEEPNRKNLIE
        MLGTSAM KITS+GRWSVFGEVI+TISTKHHK TTLED   +DKV PCSNTHETCAC  EPE CAC   SCKG V++ D+ DSSRNIPLPEEP RKNLIE
Subjt:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPESCKGPVTLDDKDDSSRNIPLPEEPNRKNLIE

Query:  WLLRRRKSHV-PKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSNW
        W+LRRRKS V PKREE E+PI +ERKQ  AGG + EWGVVD ILVGGILIS FTI GLLFH+ STTLSS+W
Subjt:  WLLRRRKSHV-PKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSNW

A0A1S3CAU3 Threonylcarbamoyladenosine tRNA methylthiotransferase

2.7e-62

76.61

Show/hide

Query:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPESCKGPVTLDDKDDSSRNIPLPEEPNRKNLIE
        MLGTSAM KITS+GRWSVFGEVI+TISTKHHK TTLED   +DKV PCSNTHETCAC  EPE CAC   SCKG V++ D+ DSSRNIPLPEEP RKNLIE
Subjt:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPESCKGPVTLDDKDDSSRNIPLPEEPNRKNLIE

Query:  WLLRRRKSHV-PKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSNW
        W+LRRRKS V PKREE E+PI +ERKQ  AGG + EWGVVD ILVGGILIS FTI GLLFH+ STTLSS+W
Subjt:  WLLRRRKSHV-PKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSNW

A0A6J1GBS1 Threonylcarbamoyladenosine tRNA methylthiotransferase

1.8e-74

85.29

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Query:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPES-CKGPVTLDDKDDSSRNIPLPEEPNRKNLI
        MLGTSA+VKITSVGRWSVFGEVI+TI TKHHKTT LEDMPIQ KVPPCS+ HETCAC +EPE CACGPES CKGPV LD+KDDSSRNIPLPEEP RKNL+
Subjt:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPES-CKGPVTLDDKDDSSRNIPLPEEPNRKNLI

Query:  EWLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSN
        EWLLR+RKSH PKREE+ESP+ SERKQ SAGG M EWGVVDRILVGGILISIFTIVGLLFHL STTLSS+
Subjt:  EWLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSN

A0A6J1KEZ5 Threonylcarbamoyladenosine tRNA methylthiotransferase

1.8e-74

85.88

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Query:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPES-CKGPVTLDDKDDSSRNIPLPEEPNRKNLI
        MLGTSA+VKITSVGRWSVFGEVI+TI TKHHKTT LEDMPIQ KVPPCSN+HETCAC +EPE CACGPES CKGPV LD+KDDSSRNIPLPEEP RKNL+
Subjt:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPES-CKGPVTLDDKDDSSRNIPLPEEPNRKNLI

Query:  EWLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSN
        EWLLR+RKSHVPKREE ESP+ S RKQ SAGG M EWGVVDRILVGGILISIFTIVGLLFHL STTLSS+
Subjt:  EWLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSN

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT1G72090.1 Methylthiotransferase

2.1e-14

36.14

Show/hide

Query:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPESCKGPVTLDDKDDSSRNIPLPEEPNRKNLIE
        MLGTSAM +ITSVGRWSVFGEVI+T S+ + +T + E+  +     PCS+   TC      E C C  ESC G     +  + S NI   ++   K+   
Subjt:  MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPESCKGPVTLDDKDDSSRNIPLPEEPNRKNLIE

Query:  WLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTL
                   K+EEKE      ++ V  G  +  WG +D+ LV G+ +S  TI+ LL  +    L
Subjt:  WLLRRRKSHVPKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTL

Sequences

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CDS sequence

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ATGCTGGGAACGTCGGCTATGGTGAAGATAACATCCGTTGGAAGGTGGTCTGTTTTTGGGGAAGTGATCAAAACCATCAGCACCAAACATCATAAAACAACTACTCTCGA
AGACATGCCGATCCAAGACAAAGTTCCTCCTTGCTCTAACACTCACGAGACATGTGCATGTCCCATGGAACCTGAACCTTGCGCTTGTGGACCAGAAAGTTGTAAAGGAC
CAGTCACTCTGGATGATAAAGATGATTCTTCAAGGAATATCCCATTGCCTGAAGAGCCAAATCGGAAGAATCTTATTGAATGGCTATTGAGGAGAAGGAAGAGCCATGTG
CCCAAAAGAGAGGAGAAAGAAAGTCCCATAGAATCTGAGAGAAAGCAGGTATCGGCTGGAGGAATCATGGGTGAGTGGGGCGTGGTTGATAGGATTCTTGTTGGTGGAAT
ACTAATCAGTATCTTTACAATTGTTGGTCTACTGTTTCATCTTCGATCCACGACACTCTCCTCTAATTGGTGA

mRNA sequence

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Protein sequence

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MLGTSAMVKITSVGRWSVFGEVIKTISTKHHKTTTLEDMPIQDKVPPCSNTHETCACPMEPEPCACGPESCKGPVTLDDKDDSSRNIPLPEEPNRKNLIEWLLRRRKSHV
PKREEKESPIESERKQVSAGGIMGEWGVVDRILVGGILISIFTIVGLLFHLRSTTLSSNW

Sgr022210 (gene) of Monk fruit (Qingpiguo) v1 genome