CuGenDBv2

Gene ID	Spg000122
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
Genome location	scaffold6:21683561..21686179
RNA-Seq Expression	Spg000122
Synteny	Spg000122
Gene Ontology terms	GO:0032981 - mitochondrial respiratory chain complex I assembly (biological process) GO:0005743 - mitochondrial inner membrane (cellular component) GO:0070469 - respirasome (cellular component)
InterPro domains	IPR007763 - NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0033972.1 NDUFA12 domain-containing protein [Cucumis melo var. makuwa]

1.4e-44

100

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Query:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
        MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
Subjt:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA

TYK02574.1 NDUFA12 domain-containing protein [Cucumis melo var. makuwa]

1.4e-44

100

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Query:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
        MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
Subjt:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA

XP_016903027.1 PREDICTED: uncharacterized protein LOC103501624 [Cucumis melo]

4.9e-45

100

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Query:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVAR
        MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVAR
Subjt:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVAR

XP_038903173.1 uncharacterized protein LOC120089839 isoform X1 [Benincasa hispida]

1.4e-44

100

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Query:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
        MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
Subjt:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA

XP_038903174.1 uncharacterized protein LOC120089839 isoform X2 [Benincasa hispida]

1.4e-44

100

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Query:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
        MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
Subjt:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA

TrEMBL top hits

e value

%identity

Alignment

A0A0A0L526 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

3.8e-43

96.7

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Query:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
        MSKLFGKIA FFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWV+FKGEDDPTSIPVEWICWLNGQRKKAPTPEEM ELEARRERVRQNVA
Subjt:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA

A0A1S4E480 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

2.3e-45

100

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Query:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVAR
        MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVAR
Subjt:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVAR

A0A5A7SS01 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

6.8e-45

100

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Query:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
        MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
Subjt:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA

A0A5D3BTS9 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

6.8e-45

100

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Query:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
        MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
Subjt:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA

A0A6J1JVV8 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

1.1e-42

95.6

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Query:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
        MSKLF KIAGFF++RTL+GVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA
Subjt:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVA

SwissProt top hits

e value

%identity

Alignment

Q54MV7 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

5.1e-05

44.07

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Query:  TLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKG--EDDPTSIPVEWICWLNGQRKKAPT
        TLVGVDK GNRY+  ++EI G     RWV +     +DPT+IP E+  W++    K P+
Subjt:  TLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKG--EDDPTSIPVEWICWLNGQRKKAPT

Q59J78 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2

7.4e-04

33.75

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Query:  RTLVGVDKAGNRYFFVKEEID---GIMKEKRWVVFKG----EDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVR
        R  VG D  GN+Y++V E  +     ++EKR V        + +   IP EW  W+   RK  PT EE+++ E  RE ++
Subjt:  RTLVGVDKAGNRYFFVKEEID---GIMKEKRWVVFKG----EDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVR

Q8N183 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2

9.6e-04

32.47

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Query:  VGVDKAGNRYFFV---KEEIDGIMKEKRWVVFKGED----DPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVR
        VG D+ GN+Y+++   K      ++EKR V    +     +   IP EW  W+   RK  PT EE+++ E  RE ++
Subjt:  VGVDKAGNRYFFV---KEEIDGIMKEKRWVVFKGED----DPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVR

Arabidopsis top hits

e value

%identity

Alignment

AT4G26965.1 NADH:ubiquinone oxidoreductase, 17.2kDa subunit

1.1e-34

60.71

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Query:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVARASGKQSLI
        MS+L+ + AG FS+++ +GVDK GN+YF   EEIDG +KEKRWV F+ E+DPTSIPVEWICWLNGQRK+APTPEEM+ELEARRERV+ NVA    ++   
Subjt:  MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVARASGKQSLI

Query:  HSMGQLGSSRTI
            ++G+ R I
Subjt:  HSMGQLGSSRTI

AT4G26965.2 NADH:ubiquinone oxidoreductase, 17.2kDa subunit

4.7e-22

65.33

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Query:  MKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVARASGKQSLIHSMGQLGSSRTI
        MKEKRWV F+ E+DPTSIPVEWICWLNGQRK+APTPEEM+ELEARRERV+ NVA    ++       ++G+ R I
Subjt:  MKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVARASGKQSLIHSMGQLGSSRTI

Sequences

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CDS sequence

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ATGTCGAAGCTTTTCGGGAAAATTGCCGGCTTCTTCAGCAATAGAACTCTGGTGGGAGTGGACAAAGCGGGAAATAGATACTTCTTTGTAAAAGAAGAAATCGATGGCAT
AATGAAAGAGAAGAGATGGGTGGTGTTTAAGGGTGAGGATGATCCAACGTCAATTCCCGTGGAATGGATATGTTGGTTAAATGGGCAGCGTAAAAAGGCTCCAACCCCAG
AGGAAATGATGGAGCTAGAAGCTAGGCGTGAACGTGTTCGACAAAATGTTGCCCGAGCTTCAGGCAAACAAAGCCTCATTCATTCGATGGGTCAGCTTGGCAGTTCAAGG
ACCATTGAGGAATGGACTTCCGAGCTAGAGGCCTTGTTCGAGTATTTGGGTGCTGATGCTCAGCAATGA

mRNA sequence

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Protein sequence

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MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVARASGKQSLIHSMGQLGSSR
TIEEWTSELEALFEYLGADAQQ

Spg000122 (gene) of Sponge gourd (cylindrica) v1 genome