CuGenDBv2

Gene ID	Spg002476
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Ribonuclease T(2)
Genome location	scaffold6:3508123..3510439
RNA-Seq Expression	Spg002476
Synteny	Spg002476
Gene Ontology terms	GO:0016070 - RNA metabolic process (biological process) GO:0003723 - RNA binding (molecular function) GO:0033897 - ribonuclease T2 activity (molecular function)
InterPro domains	IPR001568 - Ribonuclease T2-like IPR036430 - Ribonuclease T2-like superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAG7014875.1 Ribonuclease 2, partial [Cucurbita argyrosperma subsp. argyrosperma]

5.7e-30

47.85

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Query:  MAFFSIK----VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCG
        MA FSIK    V+AP VF+   +V QL     A E    GGEQREFDYF+L L WP T+C+NT  CCP NGCCRGADSPTEFTI   +   N  G   C 
Subjt:  MAFFSIK----VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCG

Query:  HNIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHE
         + +++                          L ED+EKYWPT+ CG+TS CH T GSFWAHE
Subjt:  HNIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHE

XP_022140985.1 ribonuclease 2-like [Momordica charantia]

1.7e-29

46.34

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Query:  MAFFSIK---VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGH
        MA  SIK     A  VF++  VV QLQ AAFA     G  EQREFDYF+L L WP T+C N   CCP+NGCCRGA+SPTEFTI   +      G   C  
Subjt:  MAFFSIK---VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGH

Query:  NIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ
        +                           I+SL++D++KYWPT++CG+TSTCH T GSFWAHE +
Subjt:  NIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ

XP_022922895.1 ribonuclease 2-like [Cucurbita moschata]

2.0e-30

47.88

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Query:  MAFFSIK----VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCG
        MA FSIK    V+AP VF+   +V QL     A E    GGEQREFDYF+L L WP T+C+NT  CCP NGCCRGADSPTEFTI   +   N  G   C 
Subjt:  MAFFSIK----VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCG

Query:  HNIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ
         + +                         I  L ED+EKYWPT++CG+TS CH T GSFWAHE +
Subjt:  HNIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ

XP_022984766.1 ribonuclease 2-like [Cucurbita maxima]

4.0e-31

47.88

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Query:  MAFFSIK----VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCG
        MA F IK    V+AP VF+++++  QL     A E  +GGG Q+EFDYF+L L WP T+C+NT  CCP NGCCRGADSPTEFTI   +   N  G   C 
Subjt:  MAFFSIK----VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCG

Query:  HNIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ
         + +              +ISL          LSED+EKYWPT++CG+TS CH T GSFWAHE +
Subjt:  HNIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ

XP_023553229.1 ribonuclease 2-like [Cucurbita pepo subsp. pepo]

1.3e-29

47.88

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Query:  MAFFSIK----VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCG
        MA FSIK    V+AP VF+   +V QL     A E  +GGG QREFDYF+L L WP T+C+NT  CCP NGCCRGADSPTEFTI   +   N  G   C 
Subjt:  MAFFSIK----VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCG

Query:  HNIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ
         + +              +ISL          L ED++KYWPT++CG+T  CH T GSFWAHE +
Subjt:  HNIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ

TrEMBL top hits

e value

%identity

Alignment

A0A1S3CHX1 Ribonuclease T(2)

2.9e-27

44.1

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Query:  MAFFSIKVLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGHNIM
        MA FSIK   P+VF+ + V        F+ E    G EQREFDYF+L L WP T+C+N   CCP+N CCRGADSPTEFTI   +   N  G   C  +  
Subjt:  MAFFSIKVLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGHNIM

Query:  TINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ
        + N   + +                   L+ED++KYWPT++CG++STCH T GSFWAHE +
Subjt:  TINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ

A0A5A7V3T6 Ribonuclease T(2)

4.9e-27

44.65

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Query:  MAFFSIKVLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGHNIM
        MA FSIK   P+VF+ + V        F+ E    G EQREFDYF+L L WP T+C+N   CCP+N CCRGADSPTEFTI   +   N  G   C  +  
Subjt:  MAFFSIKVLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGHNIM

Query:  TINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHE
        + N   + +                   L+ED++KYWPT++CG++STCH T GSFWAHE
Subjt:  TINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHE

A0A6J1CGP7 Ribonuclease T(2)

8.0e-30

46.34

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Query:  MAFFSIK---VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGH
        MA  SIK     A  VF++  VV QLQ AAFA     G  EQREFDYF+L L WP T+C N   CCP+NGCCRGA+SPTEFTI   +      G   C  
Subjt:  MAFFSIK---VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGH

Query:  NIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ
        +                           I+SL++D++KYWPT++CG+TSTCH T GSFWAHE +
Subjt:  NIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ

A0A6J1EA39 Ribonuclease T(2)

9.5e-31

47.88

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Query:  MAFFSIK----VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCG
        MA FSIK    V+AP VF+   +V QL     A E    GGEQREFDYF+L L WP T+C+NT  CCP NGCCRGADSPTEFTI   +   N  G   C 
Subjt:  MAFFSIK----VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCG

Query:  HNIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ
         + +                         I  L ED+EKYWPT++CG+TS CH T GSFWAHE +
Subjt:  HNIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ

A0A6J1J670 Ribonuclease T(2)

1.9e-31

47.88

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Query:  MAFFSIK----VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCG
        MA F IK    V+AP VF+++++  QL     A E  +GGG Q+EFDYF+L L WP T+C+NT  CCP NGCCRGADSPTEFTI   +   N  G   C 
Subjt:  MAFFSIK----VLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCG

Query:  HNIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ
         + +              +ISL          LSED+EKYWPT++CG+TS CH T GSFWAHE +
Subjt:  HNIMTINGLHVVLMKALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ

SwissProt top hits

e value

%identity

Alignment

P42814 Ribonuclease 2

1.0e-21

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Query:  AFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGHNIMTINGLHVVLMKALKKISLLYGPSLTI
        AFAG+ +     QREFDYF L+L WP T CR T  CC  N CCRG+D+PT+FTI   +   N      C +                K+IS         
Subjt:  AFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGHNIMTINGLHVVLMKALKKISLLYGPSLTI

Query:  ISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ---ILPSPIF
         +L + LEKYWP+  CGS S+C+   GSFW HE +      SP+F
Subjt:  ISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ---ILPSPIF

Arabidopsis top hits

e value

%identity

Alignment

AT2G39780.1 ribonuclease 2

7.2e-23

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Query:  AFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGHNIMTINGLHVVLMKALKKISLLYGPSLTI
        AFAG+ +     QREFDYF L+L WP T CR T  CC  N CCRG+D+PT+FTI   +   N      C +                K+IS         
Subjt:  AFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGHNIMTINGLHVVLMKALKKISLLYGPSLTI

Query:  ISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ---ILPSPIF
         +L + LEKYWP+  CGS S+C+   GSFW HE +      SP+F
Subjt:  ISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQ---ILPSPIF

AT2G39780.2 ribonuclease 2

1.6e-22

41.35

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Query:  AFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGHNIMTINGLHVVLMKALKKISLLYGPSLTI
        AFAG+ +     QREFDYF L+L WP T CR T  CC  N CCRG+D+PT+FTI   +   N      C +                K+IS         
Subjt:  AFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQ-FYVSLNLNGQMGCGHNIMTINGLHVVLMKALKKISLLYGPSLTI

Query:  ISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHE
         +L + LEKYWP+  CGS S+C+   GSFW HE
Subjt:  ISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHE

Sequences

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CDS sequence

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ATGGCTTTTTTCTCCATCAAAGTGTTGGCGCCGGTAGTATTTGTTGTTGTTAGTGTTGTTGCTCAGCTGCAGACGGCGGCGTTTGCGGGGGAGGGCCTCACCGGCGGCGG
GGAGCAGCGGGAGTTTGACTACTTTATATTAACTCTACTATGGCCACCCACTGCCTGCAGAAACACAAGCCTCTGTTGCCCCACCAACGGTTGCTGCCGCGGAGCTGATT
CGCCCACTGAATTTACAATTCAATTTTATGTGAGTTTGAATTTGAATGGGCAGATGGGCTGTGGCCACAATATAATGACAATCAATGGCCTTCATGTTGTACTGATGAAA
GCTTTGAAGAAAATCAGTTTACTTTATGGTCCTAGTCTTACTATTATAAGCTTGAGTGAGGATCTTGAGAAGTATTGGCCAACTTTTCAATGTGGTTCTACATCAACCTG
CCATGAGACAAATGGATCATTTTGGGCTCATGAGGTTCAAATTCTTCCCTCTCCAATCTTCTTCTTCCTTAGCTGA

mRNA sequence

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Protein sequence

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MAFFSIKVLAPVVFVVVSVVAQLQTAAFAGEGLTGGGEQREFDYFILTLLWPPTACRNTSLCCPTNGCCRGADSPTEFTIQFYVSLNLNGQMGCGHNIMTINGLHVVLMK
ALKKISLLYGPSLTIISLSEDLEKYWPTFQCGSTSTCHETNGSFWAHEVQILPSPIFFFLS

Spg002476 (gene) of Sponge gourd (cylindrica) v1 genome