CuGenDBv2

Gene ID	Spg004851
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RNase H domain-containing protein
Genome location	scaffold9:21492771..21493927
RNA-Seq Expression	Spg004851
Synteny	Spg004851
Gene Ontology terms	GO:0003676 - nucleic acid binding (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily IPR044730 - Ribonuclease H-like domain, plant type

Homology

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GenBank top hits

e value

%identity

Alignment

KAF4352389.1 hypothetical protein F8388_016668 [Cannabis sativa]

3.1e-10

31.54

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Query:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI
        P  + L+NSD +   N    G+  I R+H G  L  E +FI       + E VAIRMGL L +S  C ++++ ADC+  +N +  K    +   ++++ I
Subjt:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI

Query:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA
              F    F++ PR  N VA+ +A  A
Subjt:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA

KAF4356524.1 hypothetical protein G4B88_001072 [Cannabis sativa]

1.8e-10

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Query:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI
        P  + L+N D +   +    G+  I RDH G  + AE +F        LAE  AI+MGL L +   C+++L+  DC+  +N +  +  P +   +++++I
Subjt:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI

Query:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA
              F   DF++ PR  N VA+ +A  A
Subjt:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA

XP_030477903.1 uncharacterized protein LOC115694940 [Cannabis sativa]

3.1e-10

31.54

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Query:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI
        P  + L+NSD +   N    G+  I R+H G  L  E +FI       + E VAIRMGL L +S  C ++++ ADC+  +N +  K    +   ++++ I
Subjt:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI

Query:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA
              F    F++ PR  N VA+ +A  A
Subjt:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA

XP_030494852.1 uncharacterized protein LOC115710636 [Cannabis sativa]

1.1e-12

34.62

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Query:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI
        P  + L+NSD +   N    G+  I RDH G  + AE +FI       LAE VAIRMGL LV+   C  +L+ ADC+  +N +  K    +   ++++ I
Subjt:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI

Query:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA
              F   +F++ PR  N VA+ +A  A
Subjt:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA

XP_038902513.1 uncharacterized protein LOC120089172 [Benincasa hispida]

9.4e-15

38.21

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Query:  VNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEIWNLIPF
        +N D AW  +P S+G   I RD+ G+    +   ID  +  PLAE   +  GL+L   +  K ++V++DC  AI+  LK  +  S V   +EEIW +   
Subjt:  VNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEIWNLIPF

Query:  FRMIDFVYIPRKYNTVADCIAKR
        F  I+F YIPR  N +AD +AKR
Subjt:  FRMIDFVYIPRKYNTVADCIAKR

TrEMBL top hits

e value

%identity

Alignment

A0A7J6DL63 Uncharacterized protein

1.5e-10

31.54

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Query:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI
        P  + L+NSD +   N    G+  I R+H G  L  E +FI       + E VAIRMGL L +S  C ++++ ADC+  +N +  K    +   ++++ I
Subjt:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI

Query:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA
              F    F++ PR  N VA+ +A  A
Subjt:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA

A0A7J6E3X6 Uncharacterized protein

1.5e-10

31.54

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Query:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI
        P  + L+NSD +   N    G+  I R+H G  L  E +FI       + E VAIRMGL L +S  C ++++ ADC+  +N +  K    +   ++++ I
Subjt:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI

Query:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA
              F    F++ PR  N VA+ +A  A
Subjt:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA

A0A7J6EDW6 Uncharacterized protein

8.9e-11

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Query:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI
        P  + L+N D +   +    G+  I RDH G  + AE +F        LAE  AI+MGL L +   C+++L+  DC+  +N +  +  P +   +++++I
Subjt:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI

Query:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA
              F   DF++ PR  N VA+ +A  A
Subjt:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA

A0A803PAJ6 Uncharacterized protein

5.5e-13

34.62

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Query:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI
        P  + L+NSD +   N    G+  I RDH G  + AE +FI       LAE VAIRMGL LV+   C  +L+ ADC+  +N +  K    +   ++++ I
Subjt:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI

Query:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA
              F   +F++ PR  N VA+ +A  A
Subjt:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA

A0A803QB70 Uncharacterized protein

1.5e-10

31.54

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Query:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI
        P  + L+NSD +   N    G+  I R+H G  L  E +FI       + E VAIRMGL L +S  C ++++ ADC+  +N +  K    +   ++++ I
Subjt:  PKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEI

Query:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA
              F    F++ PR  N VA+ +A  A
Subjt:  WNLIPFFRMIDFVYIPRKYNTVADCIAKRA

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT4G29090.1 Ribonuclease H-like superfamily protein

2.0e-07

24.39

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Query:  NSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEIWNLIPFF
        N+D  W R+    G+G + R+  G      A+ +        AEL A+R  +  +       ++ E+D ++ I  +L     W  ++  ++++  L+  F
Subjt:  NSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGLKLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEIWNLIPFF

Query:  RMIDFVYIPRKYNTVADCIAKRA
          + FV+IPR+ NT+A+ +A+ +
Subjt:  RMIDFVYIPRKYNTVADCIAKRA

Sequences

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CDS sequence

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ATGGGGAATTCTTTTAAACCGGCCTATCATGCTCTATTTGAATGCCAAAGAGCAAAGGAGATATGGAAACTCACATGTGATCGAGTATTTCTGGACATCGATTTCAACGG
CAGCTTCATTAATAGATGGTTGAAAATTTGCTCAAATCTGATGCCTAAAGAACGCGATCTGGTGAATTCTGATGTCGCTTGGACAAGAAACCCTCCATCTACTGGTGTCG
GTGTCATTTGCAGAGACCACTATGGAAATTTTCTGGGTGCTGAAGCCCAGTTTATTGACTTGGATTTTGAAGGCCCATTAGCAGAATTGGTGGCTATTCGGATGGGCTTA
AAACTGGTGATATCATTGGGTTGCAAAAATTTATTGGTAGAAGCTGATTGTGAAATGGCTATTAATTTTGTCTTGAAGAAATCCTTGCCGTGGAGTTGTGTTGAATCAGT
AGTAGAAGAAATTTGGAATCTTATCCCCTTTTTCCGTATGATAGACTTTGTTTACATTCCGAGAAAGTATAATACTGTAGCAGACTGTATAGCAAAAAGGGCCTAA

mRNA sequence

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Protein sequence

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MGNSFKPAYHALFECQRAKEIWKLTCDRVFLDIDFNGSFINRWLKICSNLMPKERDLVNSDVAWTRNPPSTGVGVICRDHYGNFLGAEAQFIDLDFEGPLAELVAIRMGL
KLVISLGCKNLLVEADCEMAINFVLKKSLPWSCVESVVEEIWNLIPFFRMIDFVYIPRKYNTVADCIAKRA

Spg004851 (gene) of Sponge gourd (cylindrica) v1 genome