CuGenDBv2

Gene ID	Spg006292
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	kinetochore protein NUF2 homolog
Genome location	scaffold4:4030892..4040939
RNA-Seq Expression	Spg006292
Synteny	Spg006292
Gene Ontology terms	GO:0007052 - mitotic spindle organization (biological process) GO:0045132 - meiotic chromosome segregation (biological process) GO:0051301 - cell division (biological process) GO:0051315 - attachment of mitotic spindle microtubules to kinetochore (biological process) GO:0051383 - kinetochore organization (biological process) GO:0031262 - Ndc80 complex (cellular component) GO:0003676 - nucleic acid binding (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function) GO:0044877 - protein-containing complex binding (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR036397 - Ribonuclease H superfamily IPR044730 - Ribonuclease H-like domain, plant type

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6588803.1 Kinetochore protein NUF2-like protein, partial [Cucurbita argyrosperma subsp. sororia]

5.0e-72

86.52

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Query:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL
        QRALEEKKLAREEAKNAE+LAMQAFQEKTGIVE+YSKVSRKMLKHLAQ+QTIQEQVNSAKSVEKEFKALKAKLSDEEV+DKSL V+LVE ++KV+QLEDL
Subjt:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL

Query:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE
        RRQTEKER++KCEEA K+LNNV  EVESM+LDLE+RQ+NVEAVLMEVDA+TSKTNS+RESNVHK Q+LLHKCENI+KE
Subjt:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE

XP_022157479.1 probable kinetochore protein NUF2 [Momordica charantia]

6.5e-72

88.2

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Query:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL
        QRALEEKK AREE KNAERLAMQAFQEKTGIVE+YSKVSRKM KHLAQ+ TIQEQVNSAKSVEKEFKALKAKLSDEE+LDKSL V++VELQ+KVQQLEDL
Subjt:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL

Query:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE
        RRQTEK RD+KCEEATKDLNNV  EVESMRLDLE+RQ NVEAVLMEVDAVTSKTNSIRES+VHK Q+LLHKCE+IIKE
Subjt:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE

XP_022927739.1 probable kinetochore protein NUF2 [Cucurbita moschata]

5.0e-72

86.52

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Query:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL
        QRALEEKKLAREEAKNAE+LAMQAFQEKTGIVE+YSKVSRKMLKHLAQ+QTIQEQVNSAKSVEKEFKALKAKLSDEEV+DKSL V+LVE ++KV+QLEDL
Subjt:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL

Query:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE
        RRQTEKER++KCEEA K+LNNV  EVESM+LDLE+RQ+NVEAVLMEVDA+TSKTNS+RESNVHK Q+LLHKCENI+KE
Subjt:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE

XP_022989268.1 probable kinetochore protein NUF2 [Cucurbita maxima]

1.0e-72

87.64

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Query:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL
        QRALEEKKLAREEAKNAE+LAMQAFQEKTGIVE+YSKVSRKMLKHLAQ+QTIQEQVNSAKSVEKEFKALKAKLSDEEV+DKSL V+LVE ++KV+QLEDL
Subjt:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL

Query:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE
        RRQTEKERD+KCEEA K+LNNV  EVESM+LDLE+RQ+NVEAVLMEVDAVTSKTNS+RESNVHK Q+LLHKCENI+KE
Subjt:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE

XP_023529466.1 probable kinetochore protein NUF2 [Cucurbita pepo subsp. pepo]

2.9e-72

87.08

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Query:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL
        QRALEEKKLAREEAKNAE+LAMQAFQEKTGIVE+YSKVSRKMLKHLAQ+QTIQEQVNSAKSVEKEFKALKAKLSDEEV+DKSL V+LVE ++KV+QLEDL
Subjt:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL

Query:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE
        RRQTEKER++KCEEA K+LNNV  EVESM+LDLE+RQ+NVEAVLMEVDAVTSKTNS+RESNVHK Q+LLHKCENI+KE
Subjt:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KNL7 Uncharacterized protein

1.3e-62

79.78

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Query:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL
        QRALEEKKLAREEAKNAE+LAMQA QEKTGIVE+YSKVS+KMLKHL+ +QTI EQVNSAKSVE+EFKALK KLSDEEV DKSL V+LVELQ+KV QL+DL
Subjt:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL

Query:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE
         RQTEKERD+ CEEATKDL  V S+VES+R DLE+RQRNVEAV MEVD +TSKTNSIR+S+V K Q+LL+KCE+IIKE
Subjt:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE

A0A1S4E5E4 probable kinetochore protein NUF2

1.5e-61

78.65

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Query:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL
        QRALEEKKLAREEAKNAE+LAMQA QEKTGIVE+YSKVS+KMLKHL+ +QTI EQVNSAKSVE+EFKALK KLSDEEV DKSL V+LVELQ+KV QL+DL
Subjt:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL

Query:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE
          QTEKERD+ CEEATKDL  V S+VES+R DLE+RQRNVEAV MEVD +TSKTNSI++S+V K Q+LL+KCE+IIKE
Subjt:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE

A0A6J1DWL1 probable kinetochore protein NUF2

3.2e-72

88.2

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Query:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL
        QRALEEKK AREE KNAERLAMQAFQEKTGIVE+YSKVSRKM KHLAQ+ TIQEQVNSAKSVEKEFKALKAKLSDEE+LDKSL V++VELQ+KVQQLEDL
Subjt:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL

Query:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE
        RRQTEK RD+KCEEATKDLNNV  EVESMRLDLE+RQ NVEAVLMEVDAVTSKTNSIRES+VHK Q+LLHKCE+IIKE
Subjt:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE

A0A6J1EIG6 probable kinetochore protein NUF2

2.4e-72

86.52

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Query:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL
        QRALEEKKLAREEAKNAE+LAMQAFQEKTGIVE+YSKVSRKMLKHLAQ+QTIQEQVNSAKSVEKEFKALKAKLSDEEV+DKSL V+LVE ++KV+QLEDL
Subjt:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL

Query:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE
        RRQTEKER++KCEEA K+LNNV  EVESM+LDLE+RQ+NVEAVLMEVDA+TSKTNS+RESNVHK Q+LLHKCENI+KE
Subjt:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE

A0A6J1JNW1 probable kinetochore protein NUF2

4.9e-73

87.64

Show/hide

Query:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL
        QRALEEKKLAREEAKNAE+LAMQAFQEKTGIVE+YSKVSRKMLKHLAQ+QTIQEQVNSAKSVEKEFKALKAKLSDEEV+DKSL V+LVE ++KV+QLEDL
Subjt:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL

Query:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE
        RRQTEKERD+KCEEA K+LNNV  EVESM+LDLE+RQ+NVEAVLMEVDAVTSKTNS+RESNVHK Q+LLHKCENI+KE
Subjt:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE

SwissProt top hits

e value

%identity

Alignment

Q8RXJ0 Kinetochore protein NUF2 homolog

6.9e-32

49.44

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Query:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL
        Q ALEEKKL   E K AE+ AM  FQEK  I+E++ K  +K+LK  +Q+Q I EQV +AK+VEKEFKALK KLS++ V  KSL  ++VE +  V+QL + 
Subjt:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL

Query:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE
         +Q EKE+ +  ++ TK LN +  EVES R +LE RQ NVE+V+  VD  T+KTN +R+S   K ++L  K E I+K+
Subjt:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE

Arabidopsis top hits

e value

%identity

Alignment

AT1G61000.1 FUNCTIONS IN: molecular_function unknown; INVOLVED IN: mitosis; LOCATED IN: plasma membrane; EXPRESSED IN: cultured cell; CONTAINS InterPro DOMAIN/s: Kinetochore protein Nuf2 (InterPro:IPR005549); Has 50972 Blast hits to 29793 proteins in 2070 species: Archae - 902; Bacteria - 7404; Metazoa - 23628; Fungi - 4156; Plants - 2312; Viruses - 158; Other Eukaryotes - 12412 (source: NCBI BLink).

4.9e-33

49.44

Show/hide

Query:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL
        Q ALEEKKL   E K AE+ AM  FQEK  I+E++ K  +K+LK  +Q+Q I EQV +AK+VEKEFKALK KLS++ V  KSL  ++VE +  V+QL + 
Subjt:  QRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDL

Query:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE
         +Q EKE+ +  ++ TK LN +  EVES R +LE RQ NVE+V+  VD  T+KTN +R+S   K ++L  K E I+K+
Subjt:  RRQTEKERDMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKE

Sequences

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CDS sequence

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ATGGGGCAGAGGGCTTTGGAGGAGAAGAAATTAGCTAGGGAGGAGGCCAAGAATGCTGAAAGACTGGCAATGCAAGCTTTTCAAGAAAAGACTGGCATTGTTGAGATTTA
TTCGAAGGTTTCCAGGAAAATGTTGAAGCACCTTGCTCAGATCCAGACTATACAGGAACAGGTGAACTCTGCTAAATCTGTTGAAAAAGAATTCAAGGCCTTGAAAGCTA
AGCTTAGTGATGAGGAAGTTCTAGATAAATCGCTCCACGTCAGATTGGTTGAACTTCAATCAAAAGTGCAACAATTGGAAGATTTAAGGAGGCAGACAGAGAAGGAGAGA
GATATGAAATGCGAGGAAGCTACAAAAGATTTGAATAACGTGACGTCGGAGGTTGAATCGATGAGGCTTGATCTTGAACGAAGGCAAAGAAATGTTGAAGCAGTGCTCAT
GGAGGTCGATGCTGTGACTTCAAAAACAAATTCCATAAGAGAATCTAACGTCCACAAACATCAACAACTACTGCATAAATGCGAGAACATTATAAAAGAGGTTTTAAATC
CTTTGATTATGCATAATCCAATCAGTTGCGACCCTAATGGGAGTTCTCAATACCAGGATGATGTGGACAAAATCCAAGATATCCCAATTGCAGGAGACAACCATCCGGAC
ACTTTCTTTTGGCATCATGACAAATCAGGGAAGTATTCAGTCAGGAGTGGATATAAATTTGCAATTGATGATCTCCATGAAGCGTCCTCATCTGCAGGCTCATCTACCCT
ATCTTGGTGTCAGGAGGTATACGACCCTATAGAATGGGCATCTGCTTACATATCAGATTACAAGGAGGCCCAAGCTAAGCATCATGGAGTTAATCGAGATCAAGAGCGGC
TGAGAGATGCTGATGATTCCACTTTAGCTAATGGAGATATGGGATCAGGCATGATCATCATAAATTCGAATGGAGAACCAATGGCAACCGCAGAGTATTTCAGGCGAACG
AGTTGCTCAGTAGAGTGGACAGAAGCAAAGGCTCTTGTAGACGACATTCAGTTAGCTGTTGATTCAGGATTATCACCAGTCTGGGCAGAGACGGATTCCAAAATTGTATG
GAATCTACTTCATGATCGCGACAATTATCAGAACGAAATTGCTCCTCTAATTCACCATCTCAAGCTTTTTGCAATAGATAAGCACATCATAGGGTTTTTGCTAACAAAAG
AGAAGGTAACAAAGCGGCACATCAATTGA

mRNA sequence

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Protein sequence

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MGQRALEEKKLAREEAKNAERLAMQAFQEKTGIVEIYSKVSRKMLKHLAQIQTIQEQVNSAKSVEKEFKALKAKLSDEEVLDKSLHVRLVELQSKVQQLEDLRRQTEKER
DMKCEEATKDLNNVTSEVESMRLDLERRQRNVEAVLMEVDAVTSKTNSIRESNVHKHQQLLHKCENIIKEVLNPLIMHNPISCDPNGSSQYQDDVDKIQDIPIAGDNHPD
TFFWHHDKSGKYSVRSGYKFAIDDLHEASSSAGSSTLSWCQEVYDPIEWASAYISDYKEAQAKHHGVNRDQERLRDADDSTLANGDMGSGMIIINSNGEPMATAEYFRRT
SCSVEWTEAKALVDDIQLAVDSGLSPVWAETDSKIVWNLLHDRDNYQNEIAPLIHHLKLFAIDKHIIGFLLTKEKVTKRHIN

Spg006292 (gene) of Sponge gourd (cylindrica) v1 genome