CuGenDBv2

Gene ID	Spg008407
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	mediator of RNA polymerase II transcription subunit 15a
Genome location	scaffold10:32405490..32415274
RNA-Seq Expression	Spg008407
Synteny	Spg008407
Gene Ontology terms	GO:0006355 - regulation of transcription, DNA-templated (biological process) GO:0003713 - transcription coactivator activity (molecular function) GO:0031490 - chromatin DNA binding (molecular function)
InterPro domains	IPR036529 - Coactivator CBP, KIX domain superfamily IPR036546 - Mediator complex subunit 15, KIX domain IPR044661 - Mediator of RNA polymerase II transcription subunit 15a/b/c-like

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6571702.1 Mediator of RNA polymerase II transcription subunit 15a, partial [Cucurbita argyrosperma subsp. sororia]

2.8e-19

51.54

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Query:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK
        M+ NN RP QG EP                GIEA DWR               METLKRHLPV G +GLSEL+KIA RFEEK+YT ATSQSDYLRKISLK
Subjt:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK

Query:  MLT-----------------MVPVNEPLDS
        MLT                 MVP N+PLDS
Subjt:  MLT-----------------MVPVNEPLDS

XP_022963528.1 mediator of RNA polymerase II transcription subunit 15a-like isoform X1 [Cucurbita moschata]

2.8e-19

51.54

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Query:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK
        M+ NN RP QG EP                GIEA DWR               METLKRHLPV G +GLSEL+KIA RFEEK+YT ATSQSDYLRKISLK
Subjt:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK

Query:  MLT-----------------MVPVNEPLDS
        MLT                 MVP N+PLDS
Subjt:  MLT-----------------MVPVNEPLDS

XP_023553730.1 mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita pepo subsp. pepo]

2.8e-19

51.54

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Query:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK
        M+ NN RP QG EP                GIEA DWR               METLKRHLPV G +GLSEL+KIA RFEEK+YT ATSQSDYLRKISLK
Subjt:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK

Query:  MLT-----------------MVPVNEPLDS
        MLT                 MVP N+PLDS
Subjt:  MLT-----------------MVPVNEPLDS

XP_038887614.1 mediator of RNA polymerase II transcription subunit 15a-like [Benincasa hispida]

9.5e-20

52.31

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Query:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK
        M+ NN RP QG EP                GIEA DWR               METLKRHLPV G +GLSELRKIA RFEEK+YT ATSQSDYLRKISLK
Subjt:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK

Query:  MLT-----------------MVPVNEPLDS
        MLT                 MVP N+PLDS
Subjt:  MLT-----------------MVPVNEPLDS

XP_038887807.1 mediator of RNA polymerase II transcription subunit 15a-like [Benincasa hispida]

7.2e-20

52.31

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Query:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK
        M+ NN RP QG EP                GIEA DWR               METLKRHLPV G +GLSELRKIA RFEEK+YT ATSQSDYLRKISLK
Subjt:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK

Query:  MLT-----------------MVPVNEPLDS
        MLT                 MVP N+PLDS
Subjt:  MLT-----------------MVPVNEPLDS

TrEMBL top hits

e value

%identity

Alignment

A0A6J1HI15 mediator of RNA polymerase II transcription subunit 15a-like isoform X1

1.3e-19

51.54

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Query:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK
        M+ NN RP QG EP                GIEA DWR               METLKRHLPV G +GLSEL+KIA RFEEK+YT ATSQSDYLRKISLK
Subjt:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK

Query:  MLT-----------------MVPVNEPLDS
        MLT                 MVP N+PLDS
Subjt:  MLT-----------------MVPVNEPLDS

A0A6J1JHV7 mediator of RNA polymerase II transcription subunit 15a-like isoform X4

2.3e-19

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Query:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK
        M+ NN RPAQG E                +G++A DWR               METLKRHLPV G +GLSELRKIA RFEEK+Y  ATSQSDYLRKISLK
Subjt:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK

Query:  MLT--------------MVPVNEPLDSDEE
        MLT              MVP N+PLDS  +
Subjt:  MLT--------------MVPVNEPLDSDEE

A0A6J1JK72 mediator of RNA polymerase II transcription subunit 15a-like isoform X3

2.3e-19

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Query:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK
        M+ NN RPAQG E                +G++A DWR               METLKRHLPV G +GLSELRKIA RFEEK+Y  ATSQSDYLRKISLK
Subjt:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK

Query:  MLT--------------MVPVNEPLDSDEE
        MLT              MVP N+PLDS  +
Subjt:  MLT--------------MVPVNEPLDSDEE

A0A6J1JM75 mediator of RNA polymerase II transcription subunit 15a-like isoform X2

2.3e-19

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Query:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK
        M+ NN RPAQG E                +G++A DWR               METLKRHLPV G +GLSELRKIA RFEEK+Y  ATSQSDYLRKISLK
Subjt:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK

Query:  MLT--------------MVPVNEPLDSDEE
        MLT              MVP N+PLDS  +
Subjt:  MLT--------------MVPVNEPLDSDEE

A0A6J1JMY4 mediator of RNA polymerase II transcription subunit 15a-like isoform X1

2.3e-19

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Query:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK
        M+ NN RPAQG E                +G++A DWR               METLKRHLPV G +GLSELRKIA RFEEK+Y  ATSQSDYLRKISLK
Subjt:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR---------------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLK

Query:  MLT--------------MVPVNEPLDSDEE
        MLT              MVP N+PLDS  +
Subjt:  MLT--------------MVPVNEPLDSDEE

SwissProt top hits

e value

%identity

Alignment

F4I171 Mediator of RNA polymerase II transcription subunit 15a

9.5e-15

48.42

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Query:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLKMLTM
        M+ NN RP+         G P   TG   T +     +      METLK+HLP  GP+G++ELR+IA RFEEK+++GA +Q+DYLRKIS+KMLTM
Subjt:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLKMLTM

Arabidopsis top hits

e value

%identity

Alignment

AT1G15780.1 unknown protein

6.8e-16

48.42

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Query:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLKMLTM
        M+ NN RP+         G P   TG   T +     +      METLK+HLP  GP+G++ELR+IA RFEEK+++GA +Q+DYLRKIS+KMLTM
Subjt:  MEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWR------METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLKMLTM

AT1G15790.1 unknown protein

1.6e-09

54.9

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Query:  METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLKMLTM
        MET  + LP   P+G +ELRKIA RFEEK++  A++Q++YLR+I +KML M
Subjt:  METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLKMLTM

AT1G15790.2 unknown protein

1.6e-09

54.9

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Query:  METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLKMLTM
        MET  + LP   P+G +ELRKIA RFEEK++  A++Q++YLR+I +KML M
Subjt:  METLKRHLPVDGPDGLSELRKIAERFEEKVYTGATSQSDYLRKISLKMLTM

Sequences

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CDS sequence

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ATGGGACAGGGTTTGTCTTTCACGCATGGCGATGCTCCTCGATCCACACTCACCGTTCTCTTGCATGGCTTGCTAAATCATCTACGACCCTTTTGCAATTACGCCAAGTC
AAATTTTTTAATGGAACCAAATAATCGGAGACCTGCCCAAGGCAGAGAACCTTTCAGCGCTGCCGGAGGTCCCCAATCAACAACAGGTGGCAGCTCAACCGGAATCGAGG
CCAGCGATTGGAGAATGGAGACATTGAAGAGGCACCTTCCTGTTGATGGTCCTGATGGATTGAGTGAGCTGAGGAAGATTGCTGAAAGGTTTGAGGAAAAGGTTTATACT
GGCGCCACCAGTCAGTCAGATTACCTGAGGAAAATTTCTCTGAAAATGCTGACTATGGTGCCTGTCAATGAGCCTCTGGATTCTGATGAAGAGGATCATTATGAGCCCAA
TGATGCTAAGGTATGTTGTCGAAATTTTCGAAGCATTCGGTTAGAATTGCGTTGGTGGCTTAGTAATGTCTTTTATGTAGTAAGGTCGTTTCTTTCTGGGTTTATCTTCC
ACGCATGGCGATGCTCCTCGATCCACACTCACCGTTCTCTTGCATGGCTTGCTAAATCATCTACGACCCTTTTGCAATTACGCCAAATCTTTGAATTAGACCCCTCTTTT
CCTGCAATCGATTACTCCTCATCGCGTCAGCGTATTCAACTTGTAAGTATTTTAAAAGGTCAAACTTTTGAATGGAAACAAATAATCGGAGACCTGACCAAGGCAAAGAA
CCTTTCAGCGCTGCCGGAGGTCCCCGATCAACAACAGGTGGCAGCTCAACCGGAATCGAGGCCAGCGATTGGAGGTCTCAATTGCAGCCCGATTCTCGACAACGAATTGT
TAAGAAGATGTATAGTTACTCTGGGATGA

mRNA sequence

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Protein sequence

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MGQGLSFTHGDAPRSTLTVLLHGLLNHLRPFCNYAKSNFLMEPNNRRPAQGREPFSAAGGPQSTTGGSSTGIEASDWRMETLKRHLPVDGPDGLSELRKIAERFEEKVYT
GATSQSDYLRKISLKMLTMVPVNEPLDSDEEDHYEPNDAKVCCRNFRSIRLELRWWLSNVFYVVRSFLSGFIFHAWRCSSIHTHRSLAWLAKSSTTLLQLRQIFELDPSF
PAIDYSSSRQRIQLVSILKGQTFEWKQIIGDLTKAKNLSALPEVPDQQQVAAQPESRPAIGGLNCSPILDNELLRRCIVTLG

Spg008407 (gene) of Sponge gourd (cylindrica) v1 genome