CuGenDBv2

Gene ID	Spg008918
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	transcription elongation factor B polypeptide 3
Genome location	scaffold5:24590339..24592831
RNA-Seq Expression	Spg008918
Synteny	Spg008918
Gene Ontology terms	GO:0006368 - transcription elongation from RNA polymerase II promoter (biological process) GO:0044260 - cellular macromolecule metabolic process (biological process) GO:0070449 - elongin complex (cellular component) GO:0016462 - pyrophosphatase activity (molecular function) GO:0097159 - organic cyclic compound binding (molecular function) GO:1901363 - heterocyclic compound binding (molecular function)
InterPro domains	IPR010684 - RNA polymerase II transcription factor SIII, subunit A

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6582213.1 hypothetical protein SDJN03_22215, partial [Cucurbita argyrosperma subsp. sororia]

1.3e-36

57.05

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Query:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQK
        +GDVGQTDL+FLEHILPHCTVDQLMH+E CSKGRDL+PVTNKLWK FYERKF  D  + V+E    KE+FRWKQ+YE KMK L++K  E  +R IQN + 
Subjt:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQK

Query:  EKARKQSRQIQILEVPSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSF
        EK RKQSRQ++I E+                 + KK +   +VC VSS +N KRS+
Subjt:  EKARKQSRQIQILEVPSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSF

KAG6582214.1 hypothetical protein SDJN03_22216, partial [Cucurbita argyrosperma subsp. sororia]

1.5e-37

56.33

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Query:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQK
        +GDVGQ DL+FLEHILPHCTVDQLMH+E CSKGRDL+PVTNKLWK FYE+KF +D  + V+E    KE+FRWKQ+YE KMKEL++K  E  +R IQN + 
Subjt:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQK

Query:  EKARKQSRQIQILEVPSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSFEG
        EK RKQSRQ++I E+                 + KK K + ++C VSS +N KRS+ G
Subjt:  EKARKQSRQIQILEVPSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSFEG

KAG6582215.1 40S ribosomal protein S21-1, partial [Cucurbita argyrosperma subsp. sororia]

1.5e-37

58.49

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Query:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQK
        +GDVGQTDL+FLEHILPHCTVDQLMH+E CSKGRDL+PVTNKLWK FYERKF  D  + V+E    KE+FRWKQ+YE KMK L++K  E  +R IQN + 
Subjt:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQK

Query:  EKARKQSRQIQILEV-PSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSFEG
        EKARKQSRQ++I E+ PS               + KK +   +VC VSS +N KRS+ G
Subjt:  EKARKQSRQIQILEV-PSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSFEG

XP_022979571.1 uncharacterized protein LOC111479252 [Cucurbita maxima]

1.3e-39

58.86

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Query:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQK
        +GDVGQTDL+FLEHILPHCTVDQLMH+E CSKGRDL+P+TNKLWK FYERKF KDD + VV+    KE+FRWKQ+YE K+KEL+ K  E  +R I+N Q 
Subjt:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQK

Query:  EKARKQSRQIQILEVPSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSFEG
        EKARKQSRQ++I E+                 + KK K   +VC VSS +N KRS+ G
Subjt:  EKARKQSRQIQILEVPSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSFEG

XP_023526910.1 uncharacterized protein LOC111790279 [Cucurbita pepo subsp. pepo]

1.3e-36

57.05

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Query:  DVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQKEK
        DVG+TDL+FLEHILPHCTV+QLM +E CSKGRDL+PVTNKLWK FYERKF KD  + V+E    KE+F+WKQ+YE K KEL++K  E  +R IQN   EK
Subjt:  DVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQKEK

Query:  ARKQSRQIQILEVPSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSFEG
        ARKQSRQ++I E+    +K           + KK K + +VC VSS++N KRS+ G
Subjt:  ARKQSRQIQILEVPSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSFEG

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KJR8 Nudix hydrolase domain-containing protein

5.4e-36

56.79

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Query:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVEKETFRWKQLYEAKMKELDKKERESGERLIQNYQKEKAR
        +GDVG TDL  LE IL HCT DQL+H+E CSKGRDL+P+TNKLWKNFYERKF KDD D VV+ ETF+W  LY AKMKEL+ + ++  +R+IQ+YQKEKAR
Subjt:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVEKETFRWKQLYEAKMKELDKKERESGERLIQNYQKEKAR

Query:  KQSRQIQIL-EVPSLRNKKGRNFAKTGGNLS--------KKTKREPQVCGVSSLSNKKRSFE
        KQSRQI       SL + K     +T G  S        KK KRE  V  VS+ SNK+ + E
Subjt:  KQSRQIQIL-EVPSLRNKKGRNFAKTGGNLS--------KKTKREPQVCGVSSLSNKKRSFE

A0A6J1G8K1 transcription elongation factor B polypeptide 3 isoform X2

1.9e-33

58.11

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Query:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE-----KETFRWKQLYEAKMKELDKKERESGERLIQNYQ
        +GDVG+TDL  LE ILPHCTVDQLMHVEK S+GRDL+PVT+KLWK FYER+F +++T+ V E     K  FRW QLYEAKMK+++K E E+ +R+ Q+Y 
Subjt:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE-----KETFRWKQLYEAKMKELDKKERESGERLIQNYQ

Query:  KEKARKQSRQIQIL-EVPSLRNKK------GRNFAKTGGNLSKKTKRE
        KE ARKQSRQIQI  +VP   NK+      G N   T   L KK+K E
Subjt:  KEKARKQSRQIQIL-EVPSLRNKK------GRNFAKTGGNLSKKTKRE

A0A6J1G8W9 transcription elongation factor B polypeptide 3 isoform X1

2.5e-33

57.72

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Query:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE-----KETFRWKQLYEAKMKELDKKERESGERLIQNYQ
        +GDVG+TDL  LE ILPHCTVDQLMHVEK S+GRDL+PVT+KLWK FYER+F +++T+ V E     K  FRW QLYEAKMK+++K E E+ +R+ Q+Y 
Subjt:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE-----KETFRWKQLYEAKMKELDKKERESGERLIQNYQ

Query:  KEKARKQSRQIQIL-EVPSLRNKK-------GRNFAKTGGNLSKKTKRE
        KE ARKQSRQIQI  +VP   NK+       G N   T   L KK+K E
Subjt:  KEKARKQSRQIQIL-EVPSLRNKK-------GRNFAKTGGNLSKKTKRE

A0A6J1IR58 uncharacterized protein LOC111479252

6.1e-40

58.86

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Query:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQK
        +GDVGQTDL+FLEHILPHCTVDQLMH+E CSKGRDL+P+TNKLWK FYERKF KDD + VV+    KE+FRWKQ+YE K+KEL+ K  E  +R I+N Q 
Subjt:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQK

Query:  EKARKQSRQIQILEVPSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSFEG
        EKARKQSRQ++I E+                 + KK K   +VC VSS +N KRS+ G
Subjt:  EKARKQSRQIQILEVPSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSFEG

A0A6J1ITM2 uncharacterized protein LOC111479243

4.1e-36

56.33

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Query:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQK
        +GDVGQTDL+FLEHILPHCTV+QLM +E  SKGRDL+PVTNKLWK FYERKF KD  + V+E    KE+F+WKQ+YE K+KEL++K  E  +R IQN Q 
Subjt:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVE----KETFRWKQLYEAKMKELDKKERESGERLIQNYQK

Query:  EKARKQSRQIQILEVPSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSFEG
        EKARKQSRQ++I E+                 + KK+K   + C VSS  N KRS+ G
Subjt:  EKARKQSRQIQILEVPSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSFEG

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT2G42780.1 FUNCTIONS IN: molecular_function unknown; INVOLVED IN: regulation of transcription; LOCATED IN: integral to membrane, nucleus; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: RNA polymerase II transcription factor SIII, subunit A (InterPro:IPR010684); Has 187 Blast hits to 186 proteins in 77 species: Archae - 0; Bacteria - 0; Metazoa - 104; Fungi - 29; Plants - 38; Viruses - 0; Other Eukaryotes - 16 (source: NCBI BLink).

5.3e-20

38.1

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Query:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVEK------ETFRWKQLYEAKMKELDKKERESGERLIQNY
        +G VG  D   LE IL HCT++QL H+E  +   DLSP+T+K WK FY++ + ++D   ++E         F+W+ LYE K+  + +KE+E G RL + Y
Subjt:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVEK------ETFRWKQLYEAKMKELDKKERESGERLIQNY

Query:  QKEKARKQSRQIQILE--VPSLR-----NKKGRNFAKTGGNLSKKTK
        + E  RKQSRQ ++     PS R     +  G N      N+ KK K
Subjt:  QKEKARKQSRQIQILE--VPSLR-----NKKGRNFAKTGGNLSKKTK

AT2G42780.2 FUNCTIONS IN: molecular_function unknown; INVOLVED IN: regulation of transcription; LOCATED IN: integral to membrane, nucleus; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: RNA polymerase II transcription factor SIII, subunit A (InterPro:IPR010684).

5.3e-20

38.1

Show/hide

Query:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVEK------ETFRWKQLYEAKMKELDKKERESGERLIQNY
        +G VG  D   LE IL HCT++QL H+E  +   DLSP+T+K WK FY++ + ++D   ++E         F+W+ LYE K+  + +KE+E G RL + Y
Subjt:  MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVEK------ETFRWKQLYEAKMKELDKKERESGERLIQNY

Query:  QKEKARKQSRQIQILE--VPSLR-----NKKGRNFAKTGGNLSKKTK
        + E  RKQSRQ ++     PS R     +  G N      N+ KK K
Subjt:  QKEKARKQSRQIQILE--VPSLR-----NKKGRNFAKTGGNLSKKTK

Sequences

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CDS sequence

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ATGGGGGATGTTGGCCAAACCGATTTAGATTTTCTAGAGCACATTTTGCCACATTGCACTGTTGACCAATTGATGCATGTCGAGAAATGTTCAAAGGGCAGAGATCTCAG
TCCAGTAACCAACAAATTGTGGAAAAATTTCTACGAAAGAAAGTTCCGCAAAGATGATACTGATTTTGTAGTTGAAAAAGAGACATTTCGATGGAAGCAATTGTATGAGG
CGAAGATGAAGGAGTTGGACAAAAAGGAGAGGGAGAGTGGAGAGCGATTGATCCAAAACTACCAGAAAGAAAAAGCTCGAAAACAAAGTCGTCAAATACAGATTCTTGAG
GTTCCCTCTTTGAGGAATAAGAAAGGAAGAAACTTTGCAAAAACAGGTGGCAACCTCTCCAAGAAGACAAAGAGAGAACCACAAGTTTGTGGGGTTTCCTCTTTGAGTAA
TAAGAAACGAAGCTTTGAAGGAAGTGTTGGATTTGGATGA

mRNA sequence

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Protein sequence

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MGDVGQTDLDFLEHILPHCTVDQLMHVEKCSKGRDLSPVTNKLWKNFYERKFRKDDTDFVVEKETFRWKQLYEAKMKELDKKERESGERLIQNYQKEKARKQSRQIQILE
VPSLRNKKGRNFAKTGGNLSKKTKREPQVCGVSSLSNKKRSFEGSVGFG

Spg008918 (gene) of Sponge gourd (cylindrica) v1 genome