CuGenDBv2

Gene ID	Spg013508
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	crossover junction endonuclease EME1B isoform X1
Genome location	scaffold1:10072312..10073374
RNA-Seq Expression	Spg013508
Synteny	Spg013508
Gene Ontology terms	GO:0006281 - DNA repair (biological process) GO:0005634 - nucleus (cellular component) GO:0048476 - Holliday junction resolvase complex (cellular component) GO:0004518 - nuclease activity (molecular function)
InterPro domains	IPR033310 - Mms4/EME1/EME2

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

XP_004142045.1 crossover junction endonuclease EME1B [Cucumis sativus]

1.1e-70

70.95

Show/hide

Query:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNS-EKFAGIS
        M QPI+LSDEEDRYSTP+PI SKKRR ESD  P+I STVLVLDDDPTPQKS  NS ASFVPETPLSP PSSDVAIVKCTKA L+LHARDLNS +KFAGIS
Subjt:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNS-EKFAGIS

Query:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ
        GLICLESDNESESDSQKKKC QS+WIGSDC E+KN+G++  FDSAGSG SL                        GNDDLQEISGD KHSTFGRD+DIYQ
Subjt:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ

Query:  VDPLKAHHNI
        V       N+
Subjt:  VDPLKAHHNI

XP_022977602.1 crossover junction endonuclease EME1B isoform X1 [Cucurbita maxima]

1.7e-71

72.38

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Query:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSE-KFAGIS
        M +PIVLSDEEDRYSTPLPI SKKRR ES AFPSI STVLVLDDDPTPQKSG NS ASFVPETPLSPPPSSDV IVKCTK  LDL ARDLNS+ KFAGIS
Subjt:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSE-KFAGIS

Query:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ
        GLICLESDNESESDS KK CG S+WIGSDCD++KNLGSTS FDSAGSGPS+                        GNDDL EISGDTKHST GRD+ IYQ
Subjt:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ

Query:  VDPLKAHHNI
        V       N+
Subjt:  VDPLKAHHNI

XP_022977603.1 crossover junction endonuclease EME1B isoform X2 [Cucurbita maxima]

1.7e-71

72.38

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Query:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSE-KFAGIS
        M +PIVLSDEEDRYSTPLPI SKKRR ES AFPSI STVLVLDDDPTPQKSG NS ASFVPETPLSPPPSSDV IVKCTK  LDL ARDLNS+ KFAGIS
Subjt:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSE-KFAGIS

Query:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ
        GLICLESDNESESDS KK CG S+WIGSDCD++KNLGSTS FDSAGSGPS+                        GNDDL EISGDTKHST GRD+ IYQ
Subjt:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ

Query:  VDPLKAHHNI
        V       N+
Subjt:  VDPLKAHHNI

XP_022977604.1 crossover junction endonuclease EME1B isoform X3 [Cucurbita maxima]

1.5e-70

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Query:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSE-KFAGIS
        M +PIVLSDEEDRYSTPLPI SKKRR ES AFPSI STVLVLDDDPTPQKSG NS ASFVPETPLSPPPSSDV IVKCTK  LDL ARDLNS+ KFAGIS
Subjt:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSE-KFAGIS

Query:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ
        GLICLESDNESESDS KK CG S+WIGSDCD++KNLGSTS FDSAGSGPS+                        GNDDL EISGDTKHST GRD+ IYQ
Subjt:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ

XP_038880967.1 crossover junction endonuclease EME1B-like [Benincasa hispida]

1.7e-71

75.12

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Query:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNS-EKFAGIS
        M QPIVLSDEEDR STPLPI SKKRR ESD FP I STVLVLDDDPTPQKS RNS  SFVPETPLSPPPSSDVAIVKCTKA L+LHAR L S +KFAGIS
Subjt:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNS-EKFAGIS

Query:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ
        GLICLESDNESESDSQK+KCG S+WIGSDC E+KN+GST  FDSAGSGPSL                        GNDDLQEISGDTKHSTFG+D+DIYQ
Subjt:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ

Query:  V
        V
Subjt:  V

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KHY1 ERCC4 domain-containing protein

5.4e-71

70.95

Show/hide

Query:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNS-EKFAGIS
        M QPI+LSDEEDRYSTP+PI SKKRR ESD  P+I STVLVLDDDPTPQKS  NS ASFVPETPLSP PSSDVAIVKCTKA L+LHARDLNS +KFAGIS
Subjt:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNS-EKFAGIS

Query:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ
        GLICLESDNESESDSQKKKC QS+WIGSDC E+KN+G++  FDSAGSG SL                        GNDDLQEISGD KHSTFGRD+DIYQ
Subjt:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ

Query:  VDPLKAHHNI
        V       N+
Subjt:  VDPLKAHHNI

A0A5D3CPM4 Crossover junction endonuclease EME1B isoform X1

2.7e-70

70.95

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Query:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNS-EKFAGIS
        M QPIVLSDEEDRYSTP+PI SKKRR ESD  PSI STVLVLDDDPTPQKS  NS ASFVPETPLSP PSSDVAIVKCTKA L+LHARDLNS +KFAGIS
Subjt:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNS-EKFAGIS

Query:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ
        GLICLESDNESESDSQK+KC QS+WIGSDC E+KN+G++  FDSAGSG S                         GNDDLQEISGDTKHS FGRD DIYQ
Subjt:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ

Query:  VDPLKAHHNI
        V       N+
Subjt:  VDPLKAHHNI

A0A6J1IIZ2 crossover junction endonuclease EME1B isoform X1

8.3e-72

72.38

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Query:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSE-KFAGIS
        M +PIVLSDEEDRYSTPLPI SKKRR ES AFPSI STVLVLDDDPTPQKSG NS ASFVPETPLSPPPSSDV IVKCTK  LDL ARDLNS+ KFAGIS
Subjt:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSE-KFAGIS

Query:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ
        GLICLESDNESESDS KK CG S+WIGSDCD++KNLGSTS FDSAGSGPS+                        GNDDL EISGDTKHST GRD+ IYQ
Subjt:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ

Query:  VDPLKAHHNI
        V       N+
Subjt:  VDPLKAHHNI

A0A6J1IQH9 crossover junction endonuclease EME1B isoform X2

8.3e-72

72.38

Show/hide

Query:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSE-KFAGIS
        M +PIVLSDEEDRYSTPLPI SKKRR ES AFPSI STVLVLDDDPTPQKSG NS ASFVPETPLSPPPSSDV IVKCTK  LDL ARDLNS+ KFAGIS
Subjt:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSE-KFAGIS

Query:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ
        GLICLESDNESESDS KK CG S+WIGSDCD++KNLGSTS FDSAGSGPS+                        GNDDL EISGDTKHST GRD+ IYQ
Subjt:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ

Query:  VDPLKAHHNI
        V       N+
Subjt:  VDPLKAHHNI

A0A6J1IRV2 crossover junction endonuclease EME1B isoform X3

7.1e-71

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Query:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSE-KFAGIS
        M +PIVLSDEEDRYSTPLPI SKKRR ES AFPSI STVLVLDDDPTPQKSG NS ASFVPETPLSPPPSSDV IVKCTK  LDL ARDLNS+ KFAGIS
Subjt:  MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSE-KFAGIS

Query:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ
        GLICLESDNESESDS KK CG S+WIGSDCD++KNLGSTS FDSAGSGPS+                        GNDDL EISGDTKHST GRD+ IYQ
Subjt:  GLICLESDNESESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQ

SwissProt top hits

e value

%identity

Alignment

C5H8J1 Crossover junction endonuclease EME1B

1.2e-06

38.33

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Query:  IVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNS--TASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSEKFAGISGLI
        I++SD ED+ +TPLP  SK+ R     +P   S +L+ D DPTPQK    S  T  FVPETPL    S D ++VKC+  +  L +     +KF+G   +I
Subjt:  IVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNS--TASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSEKFAGISGLI

Query:  CLESDNESESDSQKKKCGQS
         L+S+ E     +  K  +S
Subjt:  CLESDNESESDSQKKKCGQS

Arabidopsis top hits

e value

%identity

Alignment

AT2G22140.1 essential meiotic endonuclease 1B

8.4e-08

38.33

Show/hide

Query:  IVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNS--TASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSEKFAGISGLI
        I++SD ED+ +TPLP  SK+ R     +P   S +L+ D DPTPQK    S  T  FVPETPL    S D ++VKC+  +  L +     +KF+G   +I
Subjt:  IVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNS--TASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSEKFAGISGLI

Query:  CLESDNESESDSQKKKCGQS
         L+S+ E     +  K  +S
Subjt:  CLESDNESESDSQKKKCGQS

Sequences

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CDS sequence

Show/hide CDS sequence

ATGTTCCAGCCGATCGTCCTCTCCGACGAGGAGGACCGGTACTCTACACCCCTCCCGATCCACTCCAAGAAACGCCGAATTGAATCGGATGCCTTTCCGAGCATAAACTC
TACTGTTCTTGTACTTGATGATGATCCGACCCCACAAAAATCCGGTCGCAACTCTACGGCCTCGTTCGTGCCGGAGACGCCTCTCTCTCCGCCTCCGAGTTCTGACGTCG
CCATTGTTAAATGTACGAAGGCGGCATTGGACTTGCATGCTAGGGATTTGAATTCGGAGAAGTTCGCTGGAATCAGTGGATTGATATGCTTGGAGTCCGACAATGAGTCT
GAAAGTGATTCTCAGAAGAAAAAGTGTGGGCAGAGTGATTGGATCGGTTCTGACTGTGATGAGGTGAAGAACTTAGGATCGACTTCTTTATTTGACTCGGCAGGATCGGG
ACCTTCTCTTGGTAGAAGTGTGTTCATTCTCCGGACTCCGCCATCCTCGAAAACGATCGATATTAATGGAAGTGTTTTGTCAGGGAATGATGATCTGCAGGAAATTTCTG
GGGACACGAAGCATTCAACTTTTGGGCGGGATAATGATATATATCAGGTGGATCCTCTCAAAGCGCATCATAATATCTATTTTCTTTTTCTGGAGATTGCATATGGATAG

mRNA sequence

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Protein sequence

Show/hide protein sequence

MFQPIVLSDEEDRYSTPLPIHSKKRRIESDAFPSINSTVLVLDDDPTPQKSGRNSTASFVPETPLSPPPSSDVAIVKCTKAALDLHARDLNSEKFAGISGLICLESDNES
ESDSQKKKCGQSDWIGSDCDEVKNLGSTSLFDSAGSGPSLGRSVFILRTPPSSKTIDINGSVLSGNDDLQEISGDTKHSTFGRDNDIYQVDPLKAHHNIYFLFLEIAYG

Spg013508 (gene) of Sponge gourd (cylindrica) v1 genome