CuGenDBv2

Gene ID	Spg013890
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Non-specific serine/threonine protein kinase
Genome location	scaffold3:37496015..37500732
RNA-Seq Expression	Spg013890
Synteny	Spg013890
Gene Ontology terms	GO:0006468 - protein phosphorylation (biological process) GO:0051171 - regulation of nitrogen compound metabolic process (biological process) GO:0080090 - regulation of primary metabolic process (biological process) GO:0005886 - plasma membrane (cellular component) GO:0004674 - protein serine/threonine kinase activity (molecular function) GO:0004712 - protein serine/threonine/tyrosine kinase activity (molecular function) GO:0005524 - ATP binding (molecular function) GO:0106310 - protein serine kinase activity (molecular function)
InterPro domains	IPR036691 - Endonuclease/exonuclease/phosphatase superfamily

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAG6592986.1 eIF-2-alpha kinase GCN2, partial [Cucurbita argyrosperma subsp. sororia]

1.4e-45

78.36

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Query:  VGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNLGTL
        VGC++TD+NSQ L DKVTSNE KGPYVHGYIDLFSGSGELWSWSFDMDEK  SQAQ P ADSLKLG V+E  LDKVQNLL +Q+SKR EL SPS NLGTL
Subjt:  VGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNLGTL

Query:  VEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQ
         EESED+  SITSSHSRRSLI+Q AGD  E EKQ
Subjt:  VEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQ

XP_022150409.1 eIF-2-alpha kinase GCN2 isoform X1 [Momordica charantia]

5.7e-47

51.91

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Query:  LCKVGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNL
        L  VGC+H+D NSQ L D VTS+ KKGPYVHGYIDLFSGSGELWSWSFDM+E LNSQAQ    DSLKLG V+E KL+K QNL   + SKR ELPSPSSNL
Subjt:  LCKVGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNL

Query:  GTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTDIPLDNVGDKDHVLEWAAESNPLEDLLAEEDDGSNPSVVSYS
        GTL EESEDD QSITSSHSRRSL++Q AGD I+ EKQ                                H +   A+ + L D +AE+DD  + S  S  
Subjt:  GTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTDIPLDNVGDKDHVLEWAAESNPLEDLLAEEDDGSNPSVVSYS

Query:  EDESFLSTPATKEMETMNLNAMFSE--EKACVGRG
         + SF S    +E +T N + M       AC  +G
Subjt:  EDESFLSTPATKEMETMNLNAMFSE--EKACVGRG

XP_022150410.1 eIF-2-alpha kinase GCN2 isoform X2 [Momordica charantia]

5.7e-47

51.91

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Query:  LCKVGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNL
        L  VGC+H+D NSQ L D VTS+ KKGPYVHGYIDLFSGSGELWSWSFDM+E LNSQAQ    DSLKLG V+E KL+K QNL   + SKR ELPSPSSNL
Subjt:  LCKVGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNL

Query:  GTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTDIPLDNVGDKDHVLEWAAESNPLEDLLAEEDDGSNPSVVSYS
        GTL EESEDD QSITSSHSRRSL++Q AGD I+ EKQ                                H +   A+ + L D +AE+DD  + S  S  
Subjt:  GTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTDIPLDNVGDKDHVLEWAAESNPLEDLLAEEDDGSNPSVVSYS

Query:  EDESFLSTPATKEMETMNLNAMFSE--EKACVGRG
         + SF S    +E +T N + M       AC  +G
Subjt:  EDESFLSTPATKEMETMNLNAMFSE--EKACVGRG

XP_023004333.1 eIF-2-alpha kinase GCN2 isoform X1 [Cucurbita maxima]

1.4e-45

78.36

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Query:  VGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNLGTL
        VGC++TD+NSQ L DKVTSNE KGPYVHGYIDLFSGSGELWSWSFDMDEK  SQAQ P ADSLKLG V+E  LDKVQNLL +Q+SKR EL SPS NLGTL
Subjt:  VGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNLGTL

Query:  VEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQ
         EESED+  SITSSHSRRSLI+Q AGD  E EKQ
Subjt:  VEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQ

XP_023004335.1 eIF-2-alpha kinase GCN2 isoform X2 [Cucurbita maxima]

1.4e-45

78.36

Show/hide

Query:  VGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNLGTL
        VGC++TD+NSQ L DKVTSNE KGPYVHGYIDLFSGSGELWSWSFDMDEK  SQAQ P ADSLKLG V+E  LDKVQNLL +Q+SKR EL SPS NLGTL
Subjt:  VGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNLGTL

Query:  VEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQ
         EESED+  SITSSHSRRSLI+Q AGD  E EKQ
Subjt:  VEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQ

TrEMBL top hits

e value

%identity

Alignment

A0A5A7SUN7 eIF-2-alpha kinase GCN2 isoform X4

3.4e-45

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Query:  VGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNLGTL
        VGC+HT   SQ L DK+TSNEKKGPYV+GYIDLFSGSGELWSWSFDMDEKLNSQAQ   A+SLKLG V+E KLDKVQNLL RQ+SKR EL SPSSNLGTL
Subjt:  VGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNLGTL

Query:  VEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLN
         EE+E D QSI SS+S RSLI+Q   D  EGEKQ++
Subjt:  VEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLN

A0A6J1D8E1 Non-specific serine/threonine protein kinase

2.8e-47

51.91

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Query:  LCKVGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNL
        L  VGC+H+D NSQ L D VTS+ KKGPYVHGYIDLFSGSGELWSWSFDM+E LNSQAQ    DSLKLG V+E KL+K QNL   + SKR ELPSPSSNL
Subjt:  LCKVGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNL

Query:  GTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTDIPLDNVGDKDHVLEWAAESNPLEDLLAEEDDGSNPSVVSYS
        GTL EESEDD QSITSSHSRRSL++Q AGD I+ EKQ                                H +   A+ + L D +AE+DD  + S  S  
Subjt:  GTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTDIPLDNVGDKDHVLEWAAESNPLEDLLAEEDDGSNPSVVSYS

Query:  EDESFLSTPATKEMETMNLNAMFSE--EKACVGRG
         + SF S    +E +T N + M       AC  +G
Subjt:  EDESFLSTPATKEMETMNLNAMFSE--EKACVGRG

A0A6J1D9Z7 Non-specific serine/threonine protein kinase

2.8e-47

51.91

Show/hide

Query:  LCKVGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNL
        L  VGC+H+D NSQ L D VTS+ KKGPYVHGYIDLFSGSGELWSWSFDM+E LNSQAQ    DSLKLG V+E KL+K QNL   + SKR ELPSPSSNL
Subjt:  LCKVGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNL

Query:  GTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTDIPLDNVGDKDHVLEWAAESNPLEDLLAEEDDGSNPSVVSYS
        GTL EESEDD QSITSSHSRRSL++Q AGD I+ EKQ                                H +   A+ + L D +AE+DD  + S  S  
Subjt:  GTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTDIPLDNVGDKDHVLEWAAESNPLEDLLAEEDDGSNPSVVSYS

Query:  EDESFLSTPATKEMETMNLNAMFSE--EKACVGRG
         + SF S    +E +T N + M       AC  +G
Subjt:  EDESFLSTPATKEMETMNLNAMFSE--EKACVGRG

A0A6J1KUA6 Non-specific serine/threonine protein kinase

6.8e-46

78.36

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Query:  VGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNLGTL
        VGC++TD+NSQ L DKVTSNE KGPYVHGYIDLFSGSGELWSWSFDMDEK  SQAQ P ADSLKLG V+E  LDKVQNLL +Q+SKR EL SPS NLGTL
Subjt:  VGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNLGTL

Query:  VEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQ
         EESED+  SITSSHSRRSLI+Q AGD  E EKQ
Subjt:  VEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQ

A0A6J1KZ64 Non-specific serine/threonine protein kinase

6.8e-46

78.36

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Query:  VGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNLGTL
        VGC++TD+NSQ L DKVTSNE KGPYVHGYIDLFSGSGELWSWSFDMDEK  SQAQ P ADSLKLG V+E  LDKVQNLL +Q+SKR EL SPS NLGTL
Subjt:  VGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSSNLGTL

Query:  VEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQ
         EESED+  SITSSHSRRSLI+Q AGD  E EKQ
Subjt:  VEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQ

SwissProt top hits

e value

%identity

Alignment

Q9LX30 eIF-2-alpha kinase GCN2

2.5e-05

29.88

Show/hide

Query:  VGCTHTDDNSQSLQDKVTSNEKK----GPYVHGYIDLFSGSGELWSWSFDMDEK--LNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSE--LPS
        V C     ++Q ++  + SN  K    GP+V+G+IDLFSG  +  +WS   DE   + S  Q+   D+ +   +   K DK  NL   +D  + E  LP+
Subjt:  VGCTHTDDNSQSLQDKVTSNEKK----GPYVHGYIDLFSGSGELWSWSFDMDEK--LNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSE--LPS

Query:  PSSNLGTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTD
        P + L T+ EE+ DD  SI+S  S +S       DD+E     N+ +  +++ +   +   N++
Subjt:  PSSNLGTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTD

Arabidopsis top hits

e value

%identity

Alignment

AT3G59410.1 protein kinase family protein

1.8e-06

29.88

Show/hide

Query:  VGCTHTDDNSQSLQDKVTSNEKK----GPYVHGYIDLFSGSGELWSWSFDMDEK--LNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSE--LPS
        V C     ++Q ++  + SN  K    GP+V+G+IDLFSG  +  +WS   DE   + S  Q+   D+ +   +   K DK  NL   +D  + E  LP+
Subjt:  VGCTHTDDNSQSLQDKVTSNEKK----GPYVHGYIDLFSGSGELWSWSFDMDEK--LNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSE--LPS

Query:  PSSNLGTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTD
        P + L T+ EE+ DD  SI+S  S +S       DD+E     N+ +  +++ +   +   N++
Subjt:  PSSNLGTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTD

AT3G59410.2 protein kinase family protein

1.8e-06

29.88

Show/hide

Query:  VGCTHTDDNSQSLQDKVTSNEKK----GPYVHGYIDLFSGSGELWSWSFDMDEK--LNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSE--LPS
        V C     ++Q ++  + SN  K    GP+V+G+IDLFSG  +  +WS   DE   + S  Q+   D+ +   +   K DK  NL   +D  + E  LP+
Subjt:  VGCTHTDDNSQSLQDKVTSNEKK----GPYVHGYIDLFSGSGELWSWSFDMDEK--LNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSE--LPS

Query:  PSSNLGTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTD
        P + L T+ EE+ DD  SI+S  S +S       DD+E     N+ +  +++ +   +   N++
Subjt:  PSSNLGTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTD

Sequences

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CDS sequence

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ATGCTTTCATGTCTGCTGATGGTGGTTTTCTACTTATTGTGCAAGGTTGGCTGTACTCATACAGATGACAATTCTCAATCTCTTCAAGACAAAGTAACTTCAAATGAGAA
GAAAGGACCTTATGTTCATGGCTATATAGACCTCTTCAGTGGTTCTGGGGAGTTGTGGAGCTGGAGCTTTGACATGGATGAGAAGCTCAACTCCCAAGCACAAACCCCTG
CAGCAGATAGCTTGAAACTGGGAGCAGTCCGAGAAAACAAGCTTGATAAAGTTCAAAACTTATTGGTTAGGCAAGATTCAAAGCGAAGTGAATTGCCATCTCCTTCCTCT
AACCTAGGTACCCTCGTGGAAGAAAGTGAAGATGACGGTCAGAGCATAACTTCTTCTCATTCAAGAAGATCCCTAATCCTACAGACGGCTGGAGATGATATTGAAGGTGA
GAAGCAGTTGAATAAGGGAGAAAATGGGTCGGTTGAGATGGAGGTTGGGCTAGAGTCTAAACAGAATACGGACATTCCGTTAGATAATGTGGGGGACAAAGATCACGTTT
TGGAGTGGGCTGCTGAGTCTAACCCTTTGGAGGATCTTCTTGCCGAGGAGGATGATGGCTCCAACCCTTCAGTGGTGAGTTACTCTGAGGATGAGTCGTTTTTATCGACT
CCTGCAACTAAAGAAATGGAAACCATGAATTTGAATGCCATGTTCTCGGAGGAGAAAGCCTGTGTCGGCAGAGGTGCTTCAGGATGTCTCGGTTACGGGGGATCTACCTT
AGTTGCTGATCAGGGTGAACTCGGACAAGATTCCTATCTAGATTCTTTCCAACCCTCTGGAAGTGCCTTGACTCATCCGACTGTTTATTCTGTGGGGGAGAGAATTCCGT
GCTTTGCTTCCTTAACTTGTGACTCGGGTAGTGGACTTTGGGGGGAACCTTCCCCAAATGGGCTAGTTAGCAACATCCTTTTACCTATCTATAGTCCCACAGACCTTGAA
GGCTTATCGTTCAAAGAAGGAAATAATGGATACTGGCAATTGGGGTGGGTAGCGTTGGCGTCGATTGGTATAAGGGAAGTCCCGGTTGATCATACTCCCATGAACTCCCC
AGTGAACGATAAAAAAGGCAAAAAATCGAGCAAAAAGGGAGGGGATTCTAAAAGAGTGGATAGGGAATTAAGAAAATTGGAGTCGTCCGTGAATTATGATAGGAGAAAGG
CCTCTAGGGAGGTTTGTTCTCCATGTCGATCTGGACAAAGATTTAGGGTGGGTCACTGGCACACCAGGCACAAAGGCATACAGGAGTTCTGGAACGAGCTTGGTGATCTG
TTTGGGTTGTGTGGGCATCATTGGTACATTTTGGGAGACTTTAATGTTACACGGTCCCCCTTGGAGAAGGCTTCAGGAGGGAGGGTGTCTAAATCGATGAAGTTGTTTAA
TGGGTGGATTGAGGATTGTAGCTTGTTTGATCCTCGTTTGTCTAATGGGAGATTTACCTGGGCTAATAGCAGGACGCATAGTAGAATTGATAGAGGTTTTGATTTGGAAC
ACCTCTTGAGGGTTTTGGTTTGCCCGAAACGCATGCTGCTCGAAATCGCCACCGTCGCCACCAGAACCCGCCGTCGCCTGAAACTGCCGCCGCCACCCGAACCCACCGTC
GCCTGA

mRNA sequence

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Protein sequence

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MLSCLLMVVFYLLCKVGCTHTDDNSQSLQDKVTSNEKKGPYVHGYIDLFSGSGELWSWSFDMDEKLNSQAQTPAADSLKLGAVRENKLDKVQNLLVRQDSKRSELPSPSS
NLGTLVEESEDDGQSITSSHSRRSLILQTAGDDIEGEKQLNKGENGSVEMEVGLESKQNTDIPLDNVGDKDHVLEWAAESNPLEDLLAEEDDGSNPSVVSYSEDESFLST
PATKEMETMNLNAMFSEEKACVGRGASGCLGYGGSTLVADQGELGQDSYLDSFQPSGSALTHPTVYSVGERIPCFASLTCDSGSGLWGEPSPNGLVSNILLPIYSPTDLE
GLSFKEGNNGYWQLGWVALASIGIREVPVDHTPMNSPVNDKKGKKSSKKGGDSKRVDRELRKLESSVNYDRRKASREVCSPCRSGQRFRVGHWHTRHKGIQEFWNELGDL
FGLCGHHWYILGDFNVTRSPLEKASGGRVSKSMKLFNGWIEDCSLFDPRLSNGRFTWANSRTHSRIDRGFDLEHLLRVLVCPKRMLLEIATVATRTRRRLKLPPPPEPTV
A

Spg013890 (gene) of Sponge gourd (cylindrica) v1 genome