CuGenDBv2

Gene ID	Spg015735
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Histidine--tRNA ligase
Genome location	scaffold10:20145763..20150371
RNA-Seq Expression	Spg015735
Synteny	Spg015735
Gene Ontology terms	GO:0006427 - histidyl-tRNA aminoacylation (biological process) GO:0005737 - cytoplasm (cellular component) GO:0004821 - histidine-tRNA ligase activity (molecular function) GO:0005524 - ATP binding (molecular function)
InterPro domains	IPR004516 - Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunit

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAG6604395.1 Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia]

3.7e-35

72.03

Show/hide

Query:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA
        +L  +++   RIG+TA DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+D          KKELKVVGVSE+AIEE+L+VLSVK LTKLEEI GGGGEA
Subjt:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA

Query:  LSDLKQLFSLAEMFGLTN
        +SDLKQLFSLAE FG ++
Subjt:  LSDLKQLFSLAEMFGLTN

KAG7034545.1 Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma]

2.1e-35

69.35

Show/hide

Query:  SSHSISVLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIF
        S     +L  +++   RIG+TA DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+D          KKELKVVGVSE+AIEE+L+VLSVK LTKLEEI 
Subjt:  SSHSISVLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIF

Query:  GGGGEALSDLKQLFSLAEMFGLTN
        GGGGEA+SDLKQLFSLAE FG ++
Subjt:  GGGGEALSDLKQLFSLAEMFGLTN

XP_022978407.1 histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima]

3.7e-35

72.03

Show/hide

Query:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA
        +L  +++   RIG+TA DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+D          KKELKVVGVSE+AIEE+L+VLSVK LTKLEEI GGGGEA
Subjt:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA

Query:  LSDLKQLFSLAEMFGLTN
        +SDLKQLFSLAE FG ++
Subjt:  LSDLKQLFSLAEMFGLTN

XP_023544233.1 histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo subsp. pepo]

3.7e-35

72.03

Show/hide

Query:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA
        +L  +++   RIG+TA DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+D          KKELKVVGVSE+AIEE+L+VLSVK LTKLEEI GGGGEA
Subjt:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA

Query:  LSDLKQLFSLAEMFGLTN
        +SDLKQLFSLAE FG ++
Subjt:  LSDLKQLFSLAEMFGLTN

XP_038883346.1 histidine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida]

3.7e-35

71.19

Show/hide

Query:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA
        +L  +++   R+GITA DVGFKVSSRKVLQ+VLKCYGIPEN+FGKVCII+D          KKELKVVG+SE+AIEELL+VLSVK LTKLEE+ GGGGEA
Subjt:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA

Query:  LSDLKQLFSLAEMFGLTN
        LSDLKQLFSLAE FG ++
Subjt:  LSDLKQLFSLAEMFGLTN

TrEMBL top hits

e value

%identity

Alignment

A0A5D3C5Z0 Histidine--tRNA ligase

3.0e-35

71.19

Show/hide

Query:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA
        +L  +++   R+GITA DVGFKVSSRKVLQ+VLKCYGIP+N+FGKVCII+D          KKELKVVGVSE+AIEELL+VLSVK LTKLEE+ GGGGEA
Subjt:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA

Query:  LSDLKQLFSLAEMFGLTN
        LSDLKQLFSLAE FG ++
Subjt:  LSDLKQLFSLAEMFGLTN

A0A6J1ECF7 Histidine--tRNA ligase

1.8e-35

72.03

Show/hide

Query:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA
        +L  +++   RIG+TA DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+D          KKELKVVGVSE+AIEE+L+VLSVK LTKLEEI GGGGEA
Subjt:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA

Query:  LSDLKQLFSLAEMFGLTN
        +SDLKQLFSLAE FG ++
Subjt:  LSDLKQLFSLAEMFGLTN

A0A6J1EG18 Histidine--tRNA ligase

1.8e-35

72.03

Show/hide

Query:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA
        +L  +++   RIG+TA DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+D          KKELKVVGVSE+AIEE+L+VLSVK LTKLEEI GGGGEA
Subjt:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA

Query:  LSDLKQLFSLAEMFGLTN
        +SDLKQLFSLAE FG ++
Subjt:  LSDLKQLFSLAEMFGLTN

A0A6J1IL10 Histidine--tRNA ligase

1.8e-35

72.03

Show/hide

Query:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA
        +L  +++   RIG+TA DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+D          KKELKVVGVSE+AIEE+L+VLSVK LTKLEEI GGGGEA
Subjt:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA

Query:  LSDLKQLFSLAEMFGLTN
        +SDLKQLFSLAE FG ++
Subjt:  LSDLKQLFSLAEMFGLTN

A0A6J1IMK6 Histidine--tRNA ligase

1.8e-35

72.03

Show/hide

Query:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA
        +L  +++   RIG+TA DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+D          KKELKVVGVSE+AIEE+L+VLSVK LTKLEEI GGGGEA
Subjt:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEA

Query:  LSDLKQLFSLAEMFGLTN
        +SDLKQLFSLAE FG ++
Subjt:  LSDLKQLFSLAEMFGLTN

SwissProt top hits

e value

%identity

Alignment

O82413 Histidine--tRNA ligase, chloroplastic/mitochondrial

7.4e-31

68.57

Show/hide

Query:  RIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEALSDLKQLFSL
        RIGITA DVGFKVSSRKVLQE+LK YG+PE+LFG+VCII+D          KKEL   G+SE+AIE+LL+VLSVK L  LE+I GG GEA++DLKQLFSL
Subjt:  RIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEALSDLKQLFSL

Query:  AEMFG
        AE FG
Subjt:  AEMFG

Arabidopsis top hits

e value

%identity

Alignment

AT3G46100.1 Histidyl-tRNA synthetase 1

5.3e-32

68.57

Show/hide

Query:  RIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEALSDLKQLFSL
        RIGITA DVGFKVSSRKVLQE+LK YG+PE+LFG+VCII+D          KKEL   G+SE+AIE+LL+VLSVK L  LE+I GG GEA++DLKQLFSL
Subjt:  RIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILD----------KKELKVVGVSEEAIEELLKVLSVKFLTKLEEIFGGGGEALSDLKQLFSL

Query:  AEMFG
        AE FG
Subjt:  AEMFG

AT5G03406.1 Class II aaRS and biotin synthetases superfamily protein

4.2e-13

65.38

Show/hide

Query:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK
        ++  +++   RIGITA DVGFKVSSRKVLQE LK YG+PE+LFG+VC I+DK
Subjt:  VLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK

Sequences

Show/hide sequences

CDS sequence

Show/hide CDS sequence

ATGGGAGTTTTTCTTCCTCCGATGTCTGCTGTGTTGCTTCAGTTGAATTTATTGATCAAAGAGAGCTTGTGGCAGTTAGGTGTTGTTATTCCTACGATCAAGCATTTGAC
TGAGAAGGGAGCTAAAGTGCTTCTGTCTAGCCATTCGATAAGTGTGCTTTTGGGACTTCTTTCTTTGGCTTTTCGAATTGGAATTACAGCCCCAGATGTTGGATTCAAGG
TTTCCAGTCGGAAGGTTTTGCAAGAAGTATTAAAATGCTATGGAATACCTGAAAATCTGTTTGGCAAGGTTTGCATCATCTTAGACAAGAAGGAACTGAAGGTCGTGGGT
GTATCAGAGGAGGCTATTGAGGAGCTGTTGAAAGTTCTTTCAGTAAAGTTTTTGACAAAGTTAGAAGAAATATTTGGAGGTGGTGGCGAAGCGTTGTCTGATCTAAAGCA
GCTATTTTCTCTTGCTGAAATGTTTGGGTTGACAAATGAAGAAAATATTGTAGGTAAGCAAGATTGTATGCGTGTTGGTCGCCATATTGATATTGAGATCGACCCTGAGA
TTGATCGAACCTACCATCGAAGACGAAGAAATCGGCGTGCTCGAAAACCAGTAATTGAGGAACCAAGAGAGATTGCAGCACCAACCCCACAAAATCTGTCAGCAAACCCC
ATACAAACGGCTGATGATAGAGCCAGAGGAATCAGAGATTATGCAGCCTGA

mRNA sequence

Show/hide mRNA sequence

Protein sequence

Show/hide protein sequence

MGVFLPPMSAVLLQLNLLIKESLWQLGVVIPTIKHLTEKGAKVLLSSHSISVLLGLLSLAFRIGITAPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDKKELKVVG
VSEEAIEELLKVLSVKFLTKLEEIFGGGGEALSDLKQLFSLAEMFGLTNEENIVGKQDCMRVGRHIDIEIDPEIDRTYHRRRRNRRARKPVIEEPREIAAPTPQNLSANP
IQTADDRARGIRDYAA

Spg015735 (gene) of Sponge gourd (cylindrica) v1 genome