CuGenDBv2

Gene ID	Spg017846
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Reverse transcriptase domain-containing protein
Genome location	scaffold9:35806675..35812948
RNA-Seq Expression	Spg017846
Synteny	Spg017846
Gene Ontology terms	GO:0110165 - cellular anatomical structure (cellular component)
InterPro domains	IPR026960 - Reverse transcriptase zinc-binding domain

Homology

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GenBank top hits

e value

%identity

Alignment

RVW32713.1 hypothetical protein CK203_086334 [Vitis vinifera]

2.5e-15

40.68

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Query:  LIHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQ-RFSSFVLLPQWCVLCRSQEEDLDHLLW
        L+     DSR W+  SSD FS  SFF +LS    P        LW  K+P KVK  AW V HG VN+ D++Q R     L PQWC+LC+   E +DHL  
Subjt:  LIHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQ-RFSSFVLLPQWCVLCRSQEEDLDHLLW

Query:  DFPFVCSIWSQFFRVFGI
          P    +W + F + G+
Subjt:  DFPFVCSIWSQFFRVFGI

RVX20160.1 Protein RETICULATA, chloroplastic [Vitis vinifera]

2.0e-15

40.34

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Query:  LIHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQ-RFSSFVLLPQWCVLCRSQEEDLDHLLW
        L+     DSR W+  SS  FS  SFF +LS  L P        LW  K+P KVK  AW V HG VN+ D++Q R     L PQWC+LC+   E +DHL  
Subjt:  LIHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQ-RFSSFVLLPQWCVLCRSQEEDLDHLLW

Query:  DFPFVCSIWSQFFRVFGIA
          P    +W + F + G++
Subjt:  DFPFVCSIWSQFFRVFGIA

TYK29954.1 reverse transcriptase [Cucumis melo var. makuwa]

1.5e-15

37.59

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Query:  GRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQW----CVLCRSQEEDLDHLLWD
        G R+   W++  S GFS  S F SL     P    +F ++W++K+ KKV+FF W+VL    N  DR+ R       P+W    C+LCR  +EDLDHLLWD
Subjt:  GRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQW----CVLCRSQEEDLDHLLWD

Query:  FPFVCSIWSQFFRVFGIALARNSDFFSMFEEIL
          +  ++W  F + F + LA         EE L
Subjt:  FPFVCSIWSQFFRVFGIALARNSDFFSMFEEIL

XP_022968893.1 epimerase family protein SDR39U1 homolog, chloroplastic-like [Cucurbita maxima]

5.1e-16

41.54

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Query:  IHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQWCVLCRSQEEDLDHLLWDF
        +H G R+  FW+   S G  C SFF  LS+    P AS  S   KV + KK++FF W+ LHG VN+   IQ+ SSF+L PQWC       E+LDH++   
Subjt:  IHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQWCVLCRSQEEDLDHLLWDF

Query:  PFVCSIWSQFFRVFGIALARNSDFFSMFEE
         F    W  F   FG+ LAR  D   M  E
Subjt:  PFVCSIWSQFFRVFGIALARNSDFFSMFEE

XP_038887741.1 epimerase family protein SDR39U1 homolog, chloroplastic isoform X1 [Benincasa hispida]

4.6e-17

43.85

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Query:  NMLLDDLIHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQWCVLCRSQEEDL
        ++L D  +H  RR+  FW+   S G SC     S S  L  P  S   +L KVKI K V FFAW+ LHG VN+   IQ  SSF+L  QWC LC    E+L
Subjt:  NMLLDDLIHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQWCVLCRSQEEDL

Query:  DHLLWDFPFVCSIWSQFFRVFGIALARNSD
        DH+     F  S W+ F   FG+ LAR  D
Subjt:  DHLLWDFPFVCSIWSQFFRVFGIALARNSD

TrEMBL top hits

e value

%identity

Alignment

A0A438DB76 zf-RVT domain-containing protein

1.2e-15

40.68

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Query:  LIHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQ-RFSSFVLLPQWCVLCRSQEEDLDHLLW
        L+     DSR W+  SSD FS  SFF +LS    P        LW  K+P KVK  AW V HG VN+ D++Q R     L PQWC+LC+   E +DHL  
Subjt:  LIHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQ-RFSSFVLLPQWCVLCRSQEEDLDHLLW

Query:  DFPFVCSIWSQFFRVFGI
          P    +W + F + G+
Subjt:  DFPFVCSIWSQFFRVFGI

A0A438KG54 Protein RETICULATA, chloroplastic

9.4e-16

40.34

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Query:  LIHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQ-RFSSFVLLPQWCVLCRSQEEDLDHLLW
        L+     DSR W+  SS  FS  SFF +LS  L P        LW  K+P KVK  AW V HG VN+ D++Q R     L PQWC+LC+   E +DHL  
Subjt:  LIHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQ-RFSSFVLLPQWCVLCRSQEEDLDHLLW

Query:  DFPFVCSIWSQFFRVFGIA
          P    +W + F + G++
Subjt:  DFPFVCSIWSQFFRVFGIA

A0A5D3E255 Reverse transcriptase

7.2e-16

37.59

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Query:  GRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQW----CVLCRSQEEDLDHLLWD
        G R+   W++  S GFS  S F SL     P    +F ++W++K+ KKV+FF W+VL    N  DR+ R       P+W    C+LCR  +EDLDHLLWD
Subjt:  GRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQW----CVLCRSQEEDLDHLLWD

Query:  FPFVCSIWSQFFRVFGIALARNSDFFSMFEEIL
          +  ++W  F + F + LA         EE L
Subjt:  FPFVCSIWSQFFRVFGIALARNSDFFSMFEEIL

A0A6J1HZE6 epimerase family protein SDR39U1 homolog, chloroplastic-like

2.5e-16

41.54

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Query:  IHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQWCVLCRSQEEDLDHLLWDF
        +H G R+  FW+   S G  C SFF  LS+    P AS  S   KV + KK++FF W+ LHG VN+   IQ+ SSF+L PQWC       E+LDH++   
Subjt:  IHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQWCVLCRSQEEDLDHLLWDF

Query:  PFVCSIWSQFFRVFGIALARNSDFFSMFEE
         F    W  F   FG+ LAR  D   M  E
Subjt:  PFVCSIWSQFFRVFGIALARNSDFFSMFEE

A5AUH3 Reverse transcriptase domain-containing protein

3.6e-15

39.83

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Query:  LIHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQ-RFSSFVLLPQWCVLCRSQEEDLDHLLW
        L+     DSR W+  SS  FS  SFF +LS    P        LW  K+P KVK  AW V HG VN+ D++Q R     L PQWC+LC+   E +DHL  
Subjt:  LIHHGRRDSRFWTSKSSDGFSCASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQ-RFSSFVLLPQWCVLCRSQEEDLDHLLW

Query:  DFPFVCSIWSQFFRVFGI
          P    +W + F + G+
Subjt:  DFPFVCSIWSQFFRVFGI

SwissProt top hits

e value

%identity

Alignment

P0C2F6 Putative ribonuclease H protein At1g65750

2.7e-07

29.53

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Query:  RVSPYVDNYCN---NMLLDDLIHHGRRDSRFWTSKSSDGFSCASFFRSLST-SLPPPG-ASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFV
        ++ PY  N        ++ DL+  G RD   W       FS  S +  L+   +P P  AS F+ LWKV++P++VK F W V +  V +++  +R    +
Subjt:  RVSPYVDNYCN---NMLLDDLIHHGRRDSRFWTSKSSDGFSCASFFRSLST-SLPPPG-ASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFV

Query:  LLPQWCVLCRSQEEDLDHLLWDFPFVCSIWSQFFRVFGIALARNSDFFS
             C +C+   E + H+L D P    IW     V  +   R   FFS
Subjt:  LLPQWCVLCRSQEEDLDHLLWDFPFVCSIWSQFFRVFGIALARNSDFFS

Arabidopsis top hits

e value

%identity

Alignment

AT1G43730.1 RNA-directed DNA polymerase (reverse transcriptase)-related family protein

4.8e-04

27.52

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Query:  DSRFWTSKSSDGFSCASFFRSLSTSLP--PPGASI--FSSLW-KVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQWCVLCRSQEEDLDHLLWDFP
        DS  W    +D  + ++ F +  TSL   P    +  + ++W K  +PK   F  W V    ++++DR++ +   + +P  C+LC S +E   HL ++ P
Subjt:  DSRFWTSKSSDGFSCASFFRSLSTSLP--PPGASI--FSSLW-KVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQWCVLCRSQEEDLDHLLWDFP

Query:  FVCSIWSQF
        F  ++W  F
Subjt:  FVCSIWSQF

AT3G25270.1 Ribonuclease H-like superfamily protein

8.8e-06

31.58

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Query:  PPPG-ASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQWCVLCRSQEEDLDHLLWDFPFVCSIW
        PPPG A I + +WK+K   K+K F W++L G + + D ++R    +     C  C  ++E   HL +D  +   +W
Subjt:  PPPG-ASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQWCVLCRSQEEDLDHLLWDFPFVCSIW

Sequences

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CDS sequence

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ATGCAGATTCCGACTAATTTCAGATCTATTTTCTTCGTCTTCAGATTTCCGAAAGGTTTTGATCTTTTGATTGTGCACGGTCGAGGTCGAACCAGAATTAACAATGGGAT
TCTTGGTTACGTCCCTGATTTTCGCCGTGATTGGAGTCATCGCATTTATCTGGTCAAGCATTATATAGTTCTTGGTTTAAGTATACAAGTGACTATCTTGGCGTTCAGGG
TTTCACCTTACGTTGATAATTACTGCAACAATATGCTGCTGGATGATTTGATTCATCATGGGAGAAGGGACTCTCGGTTTTGGACCTCGAAGTCTTCTGATGGCTTCTCT
TGTGCTTCTTTTTTCCGTTCTCTTTCTACATCTCTCCCTCCTCCCGGAGCCTCCATTTTCTCCTCGTTATGGAAGGTCAAAATTCCCAAGAAAGTGAAGTTCTTTGCCTG
GGAAGTTTTACATGGTTGGGTGAATTCTCAGGATCGGATCCAAAGGTTCTCCTCCTTCGTGTTGCTGCCGCAGTGGTGTGTCCTCTGTAGGAGTCAAGAGGAGGACCTTG
ATCATTTGCTTTGGGATTTTCCGTTTGTCTGTTCTATTTGGAGTCAATTCTTCAGAGTGTTTGGAATTGCTTTGGCTCGTAACAGTGACTTCTTTTCTATGTTTGAGGAA
ATCTTGTGGCATCCTCCTTTTTGTGACAAAGGGAGAGTTCTTTGGACCTCAAAATCAGGGAAAGGAAAAGGCCTCAATGTCTGTTCTGGAGTTTCCTTTCAGATTATAAA
GATATCAAATTCACTTCAACAAATTGCAATCATACACTGGCACTTTCACATTTCAGGACAACCTGTTATCGAATCTTTGAATTTGGCTACTCTTGTTACAGCATCTATAA
GTGAAGTTTATTTTGGAACTGGTACTACTAGTTGTCTTATGAATCTAAATCATCGTGGGGTTGCTGCTGATCAACCCTAA

mRNA sequence

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Protein sequence

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MQIPTNFRSIFFVFRFPKGFDLLIVHGRGRTRINNGILGYVPDFRRDWSHRIYLVKHYIVLGLSIQVTILAFRVSPYVDNYCNNMLLDDLIHHGRRDSRFWTSKSSDGFS
CASFFRSLSTSLPPPGASIFSSLWKVKIPKKVKFFAWEVLHGWVNSQDRIQRFSSFVLLPQWCVLCRSQEEDLDHLLWDFPFVCSIWSQFFRVFGIALARNSDFFSMFEE
ILWHPPFCDKGRVLWTSKSGKGKGLNVCSGVSFQIIKISNSLQQIAIIHWHFHISGQPVIESLNLATLVTASISEVYFGTGTTSCLMNLNHRGVAADQP

Spg017846 (gene) of Sponge gourd (cylindrica) v1 genome