CuGenDBv2

Gene ID	Spg018697
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Reverse transcriptase domain-containing protein
Genome location	scaffold3:10629049..10631456
RNA-Seq Expression	Spg018697
Synteny	Spg018697
Gene Ontology terms	GO:0071704 - organic substance metabolic process (biological process) GO:0003676 - nucleic acid binding (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily IPR044730 - Ribonuclease H-like domain, plant type

Homology

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GenBank top hits

e value

%identity

Alignment

XP_022143535.1 uncharacterized protein LOC111013412 [Momordica charantia]

5.4e-13

34.31

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Query:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISEL
        WK P SN WKLNT+A+W A++  GG+GW +RD  G ++    +      +I  LE +AI EG++ I     +H   + +ESDS   I  ++   +  +E+
Subjt:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISEL

Query:  SNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRA
          +++ I         +  RH  REAN +AH +A+RA
Subjt:  SNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRA

XP_022148737.1 uncharacterized protein LOC111017329 [Momordica charantia]

1.7e-11

35.82

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Query:  PSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISELSNII
        P N WKLNTDA+W AN G GGLGW +R+    +   G Q      DI  LE +AI  GI+ + S+ S   + L +ES+S   I  I G  + ++E+  ++
Subjt:  PSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISELSNII

Query:  DLIVSAAAGPTPMVFRHCGREANALAHCVAQRAS
          I +         F+H  R+ N++A  +A RA+
Subjt:  DLIVSAAAGPTPMVFRHCGREANALAHCVAQRAS

XP_022155262.1 uncharacterized protein LOC111022403 [Momordica charantia]

3.7e-14

33.33

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Query:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISEL
        W+ PP ++W LN DASW  ++ RGG+GW +R  +G ++  G ++ +   ++ +LEA AILEG++ + +        L +E+DS  V   +N   E +++ 
Subjt:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISEL

Query:  SNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRAS
          +++ I++       + F   GRE N  AH +AQRAS
Subjt:  SNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRAS

XP_022156777.1 uncharacterized protein LOC111023608 [Momordica charantia]

7.0e-13

34.51

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Query:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIAST-C----SQHQMGLEVESDSKVVIDAINGDSE
        WK P SN WKLNTDA+W A++  GG+GW +RD  G ++    +      +I  LE +AI EG++ I    C     +H   + +ESDS   I  ++   +
Subjt:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIAST-C----SQHQMGLEVESDSKVVIDAINGDSE

Query:  IISELSNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRA
          +E+  +++ I         +  RH  REAN +AH +A+RA
Subjt:  IISELSNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRA

XP_031096851.1 uncharacterized protein LOC116001092 [Ipomoea triloba]

1.1e-10

38.13

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Query:  KPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISELSN
        +PPS M KLNTDA+   N+ R G G+ +RDS G  + V     D  +  D  E++AI E +K I +    H   L VESD ++ I+ I  D  + S L  
Subjt:  KPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISELSN

Query:  IIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRASSPS
        I D I   A+  T + F    R AN +AHC+A+ + S S
Subjt:  IIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRASSPS

TrEMBL top hits

e value

%identity

Alignment

A0A2I4H9M5 uncharacterized protein LOC109014810

9.3e-11

30.99

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Query:  TWKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISE
        TW +PP+N +KLN D +   +  + G+G  +RDS+G L     +  +++ +++I+EA A+L G++   +   Q+   LE+ESD + +++ +N ++     
Subjt:  TWKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISE

Query:  LSNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRASSPS
        +  II  I           F+HCGR +NA  H +AQ+A + S
Subjt:  LSNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRASSPS

A0A6J1CP26 uncharacterized protein LOC111013412

2.6e-13

34.31

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Query:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISEL
        WK P SN WKLNT+A+W A++  GG+GW +RD  G ++    +      +I  LE +AI EG++ I     +H   + +ESDS   I  ++   +  +E+
Subjt:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISEL

Query:  SNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRA
          +++ I         +  RH  REAN +AH +A+RA
Subjt:  SNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRA

A0A6J1D5W1 uncharacterized protein LOC111017329

8.4e-12

35.82

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Query:  PSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISELSNII
        P N WKLNTDA+W AN G GGLGW +R+    +   G Q      DI  LE +AI  GI+ + S+ S   + L +ES+S   I  I G  + ++E+  ++
Subjt:  PSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISELSNII

Query:  DLIVSAAAGPTPMVFRHCGREANALAHCVAQRAS
          I +         F+H  R+ N++A  +A RA+
Subjt:  DLIVSAAAGPTPMVFRHCGREANALAHCVAQRAS

A0A6J1DNV9 uncharacterized protein LOC111022403

1.8e-14

33.33

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Query:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISEL
        W+ PP ++W LN DASW  ++ RGG+GW +R  +G ++  G ++ +   ++ +LEA AILEG++ + +        L +E+DS  V   +N   E +++ 
Subjt:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISEL

Query:  SNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRAS
          +++ I++       + F   GRE N  AH +AQRAS
Subjt:  SNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRAS

A0A6J1DSV1 uncharacterized protein LOC111023608

3.4e-13

34.51

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Query:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIAST-C----SQHQMGLEVESDSKVVIDAINGDSE
        WK P SN WKLNTDA+W A++  GG+GW +RD  G ++    +      +I  LE +AI EG++ I    C     +H   + +ESDS   I  ++   +
Subjt:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIAST-C----SQHQMGLEVESDSKVVIDAINGDSE

Query:  IISELSNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRA
          +E+  +++ I         +  RH  REAN +AH +A+RA
Subjt:  IISELSNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRA

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT2G34320.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

2.1e-07

27.27

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Query:  VTWKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQ-HQMGLEVESDSKVVIDAINGDSEII
        V WK PP    K NTDA+W   + R G+GW +R+ +G +L +G +   +    ++LE  A LE ++    T S+ +   +  ESD++ +++ +N D +  
Subjt:  VTWKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQ-HQMGLEVESDSKVVIDAINGDSEII

Query:  SELSNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRASSPSAFDGDDFFVL
          L   ++ I         + F    R  N +A  +A+ + S S +D   F ++
Subjt:  SELSNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRASSPSAFDGDDFFVL

AT4G29090.1 Ribonuclease H-like superfamily protein

1.7e-09

32.41

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Query:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEV-ESDSKVVIDAINGDSEIISE
        W+ PP    K NTDA+W  ++ R G+GW +R+  G +  +G +   K     +LE  A LE ++    + S+ Q    + ESDS+V+I+ +N D EI   
Subjt:  WKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEV-ESDSKVVIDAINGDSEIISE

Query:  LSNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRASSPSAFD
        L   I  +    +  T + F    RE N LA  VA+ + S   +D
Subjt:  LSNIIDLIVSAAAGPTPMVFRHCGREANALAHCVAQRASSPSAFD

Sequences

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CDS sequence

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GCGAGTCACGTCACTTGGAAAAAGCCCCCCTCGAACATGTGGAAACTGAACACGGATGCATCCTGGTTAGCGAATTCGGGCAGAGGAGGCTTGGGATGGACGGTGCGTGA
CTCGAACGGGTCCTTGCTCTGTGTCGGCATGCAATACACGGATAAATATTGGGACATTGATATTCTGGAAGCATTAGCGATCCTTGAAGGGATTAAATGCATCGCTAGTA
CCTGCTCTCAACACCAAATGGGGTTGGAAGTTGAATCGGACTCGAAGGTTGTAATCGATGCCATCAACGGCGACTCAGAAATCATCTCGGAGCTGAGCAACATCATCGAC
TTGATTGTGTCAGCAGCTGCAGGTCCGACTCCGATGGTCTTCAGGCATTGCGGAAGGGAAGCCAACGCACTCGCCCATTGCGTTGCCCAGCGTGCCTCGTCTCCTTCTGC
TTTCGACGGAGATGATTTTTTTGTTCTCAGTCGAGGGTCTTCTCCTTCGCGGGAAATGAGTTGTATTTTTTGGAATCCTGACTTTCCAGGGTGGATTTTCCCTCTTATTG
ATGAGGGTAGCTGTTTTTCGATTTCTGTGATTCACAAAGACGTTGGTGCAGGGCTTGTTCAATCATCCGCCAGGATCTGTACGTACAATGGCTGCCAACTTGTAGAACTG
GGGCACGTGGCAGGAGGAGAGCCCGGATGCAGGGACACTGCCAGGTTGCGGTGGGTGAGAAGGACGCCTGCTTTTGAAAGTGCCTAG

mRNA sequence

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Protein sequence

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ASHVTWKKPPSNMWKLNTDASWLANSGRGGLGWTVRDSNGSLLCVGMQYTDKYWDIDILEALAILEGIKCIASTCSQHQMGLEVESDSKVVIDAINGDSEIISELSNIID
LIVSAAAGPTPMVFRHCGREANALAHCVAQRASSPSAFDGDDFFVLSRGSSPSREMSCIFWNPDFPGWIFPLIDEGSCFSISVIHKDVGAGLVQSSARICTYNGCQLVEL
GHVAGGEPGCRDTARLRWVRRTPAFESA

Spg018697 (gene) of Sponge gourd (cylindrica) v1 genome