CuGenDBv2

Gene ID	Spg019828
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	tRNA (guanine(9)-N(1))-methyltransferase
Genome location	scaffold5:32481809..32484687
RNA-Seq Expression	Spg019828
Synteny	Spg019828
Gene Ontology terms	GO:0002939 - tRNA N1-guanine methylation (biological process) GO:0005634 - nucleus (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0000049 - tRNA binding (molecular function) GO:0052905 - tRNA (guanine(9)-N(1))-methyltransferase activity (molecular function)
InterPro domains	IPR007356 - tRNA (guanine-N1-)-methyltransferase, eukaryotic

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6583717.1 hypothetical protein SDJN03_19649, partial [Cucurbita argyrosperma subsp. sororia]

1.4e-48

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Query:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE
        MEA E+DQSAK+DPP SPS     EPPLSKNARKKLLKQQRYEAKKAEKKAQ KE KKKDIERKRKEWE+TLASVTEEER+KLIESR++LRKERMGKRSE
Subjt:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE

Query:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR
        E+EKKIERLNRAK  GQ IVIDLEFS LMTPSEIHSLVQQ   C   +GR
Subjt:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR

XP_022927615.1 tRNA (guanine(9)-N1)-methyltransferase [Cucurbita moschata]

1.4e-48

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Query:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE
        MEA E+DQSAK+DPP SPS     EPPLSKNARKKLLKQQRYEAKKAEKKAQ KE KKKDIERKRKEWE+TLASVTEEER+KLIESR++LRKERMGKRSE
Subjt:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE

Query:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR
        E+EKKIERLNRAK  GQ IVIDLEFS LMTPSEIHSLVQQ   C   +GR
Subjt:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR

XP_022973151.1 tRNA (guanine(9)-N1)-methyltransferase [Cucurbita maxima]

4.3e-50

83.33

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Query:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE
        MEAIE+DQSAK+DPP SPS     EPPLSKNARKKLLKQQRYEAKKAEKKAQ KE KKKDIERKRKEWEDTLASVTEEER+KLIESRR+LRKERMGKRSE
Subjt:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE

Query:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR
        E+EKKIERLNRAK  GQ I+IDLEFS LMTPSEIHSLVQQ   C   +GR
Subjt:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR

XP_023520714.1 tRNA (guanine(9)-N1)-methyltransferase [Cucurbita pepo subsp. pepo]

4.3e-50

83.33

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Query:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE
        MEAIE+DQSAK+DPP SPS     EPPLSKNARKKLLKQQRYEAKKAEKKAQ KELKKKDIERKRKEWE+TLASVTEEER+KLIESR++LRKERMGKRSE
Subjt:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE

Query:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR
        E+EKKIERLNRAK  GQ IVIDLEFS LMTPSEIHSLVQQ   C   +GR
Subjt:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR

XP_038893739.1 tRNA (guanine(9)-N1)-methyltransferase [Benincasa hispida]

1.4e-51

84.67

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Query:  MEAIEDDQSAKVDPPSS-----PSEPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE
        MEA+E+DQSAK+DPP S     P EPPLSKNA+KKLLKQQRYEAKKAEKK QAKE KKKDIERKRKEWEDTLASVTEEERSKLIESRR+LRKERMGKRSE
Subjt:  MEAIEDDQSAKVDPPSS-----PSEPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE

Query:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR
        EREKKIERLNRAK  GQNIVIDLEFSHLMTPSEIHSLVQQ   C   +GR
Subjt:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR

TrEMBL top hits

e value

%identity

Alignment

A0A5D3CW30 tRNA (Guanine(9)-N1)-methyltransferase isoform X1

3.1e-46

78.67

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Query:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE
        ME +E   S K+DPP SPS     EP LSKNARKKLLKQQRYEAKKA+KK QAKE KKKDIERKRKEWE+ LA VTEEER+KLIESRRNLRKERMGKRSE
Subjt:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE

Query:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR
        +REKKIERLN AK  GQNIVIDLEFSHLMTPSEIHSLVQQ   C   +GR
Subjt:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR

A0A6J1DYJ8 tRNA (guanine(9)-N(1))-methyltransferase

1.8e-46

80.95

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Query:  MEAIEDDQSAKVDPPSS--PSEPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSEERE
        MEA+E+D  AK+DP S+  P E PLSKNARKKL KQQRYEAKKAEKKAQAKE KKKDIERKRKEWE+ LASV+EEERSKLIESRR+LRKERMGKRSEERE
Subjt:  MEAIEDDQSAKVDPPSS--PSEPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSEERE

Query:  KKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR
        KK+ERLNRAK  GQNIVIDLEFSHLMTPSEI+SLVQQ   C   +GR
Subjt:  KKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR

A0A6J1DZL7 tRNA (guanine(9)-N(1))-methyltransferase

1.8e-46

80.95

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Query:  MEAIEDDQSAKVDPPSS--PSEPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSEERE
        MEA+E+D  AK+DP S+  P E PLSKNARKKL KQQRYEAKKAEKKAQAKE KKKDIERKRKEWE+ LASV+EEERSKLIESRR+LRKERMGKRSEERE
Subjt:  MEAIEDDQSAKVDPPSS--PSEPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSEERE

Query:  KKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR
        KK+ERLNRAK  GQNIVIDLEFSHLMTPSEI+SLVQQ   C   +GR
Subjt:  KKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR

A0A6J1EPG7 tRNA (guanine(9)-N(1))-methyltransferase

6.8e-49

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Query:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE
        MEA E+DQSAK+DPP SPS     EPPLSKNARKKLLKQQRYEAKKAEKKAQ KE KKKDIERKRKEWE+TLASVTEEER+KLIESR++LRKERMGKRSE
Subjt:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE

Query:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR
        E+EKKIERLNRAK  GQ IVIDLEFS LMTPSEIHSLVQQ   C   +GR
Subjt:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR

A0A6J1I7W5 tRNA (guanine(9)-N(1))-methyltransferase

2.1e-50

83.33

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Query:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE
        MEAIE+DQSAK+DPP SPS     EPPLSKNARKKLLKQQRYEAKKAEKKAQ KE KKKDIERKRKEWEDTLASVTEEER+KLIESRR+LRKERMGKRSE
Subjt:  MEAIEDDQSAKVDPPSSPS-----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSE

Query:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR
        E+EKKIERLNRAK  GQ I+IDLEFS LMTPSEIHSLVQQ   C   +GR
Subjt:  EREKKIERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGR

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT5G47680.1 FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: tRNA (guanine-N1-)-methyltransferase (InterPro:IPR016009), tRNA (guanine-N(1)-)-methyltransferase, metazoa (InterPro:IPR016653), tRNA (guanine-N1-)-methyltransferase, eukaryotic (InterPro:IPR007356); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

1.2e-37

66.44

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Query:  DDQSAKVDPPSSPS----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSEEREKKI
        DD      P S P+     PPLSKNA+KK LKQQRYEAKKAEKKAQ KE K+K+ ERK KEWE+TLA+ TEEER KLIESR++LRKERM KRSEE+EKKI
Subjt:  DDQSAKVDPPSSPS----EPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSEEREKKI

Query:  ERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGRDT
        ERLN+AK  GQ IV+D++F+HLM+ SEI SLVQQ   C   +GR T
Subjt:  ERLNRAKHSGQNIVIDLEFSHLMTPSEIHSLVQQ---CLVGDGRDT

Sequences

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CDS sequence

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ATGGAGGCCATAGAAGATGACCAGAGCGCCAAAGTGGATCCTCCTTCTTCCCCCTCAGAACCTCCCCTCTCGAAGAATGCAAGGAAGAAGCTACTGAAGCAACAGCGATA
CGAAGCGAAGAAAGCGGAGAAGAAAGCTCAGGCGAAAGAGCTGAAGAAGAAAGACATCGAGCGGAAACGAAAGGAATGGGAGGACACACTGGCGAGTGTTACCGAGGAGG
AGAGATCGAAGTTGATCGAGTCTCGGAGAAATTTGAGAAAGGAGAGGATGGGGAAGAGGTCGGAGGAGAGGGAGAAGAAGATAGAGAGGCTCAACAGGGCCAAACACTCT
GGCCAAAATATTGTCATCGACCTTGAATTTTCTCACCTCATGACCCCCTCGGAGATTCACAGTCTTGTTCAGCAGTGTTTGGTAGGTGATGGGAGAGATACTTTTTTCTG
GGAGGATAAGTGGTTGGGGGATAGACACCTCTACTCTCGTTTCCTTCATTTGCATCACTTATCCTCGATGAAGAACCACTTGGTGGTGGATGTGTTGCCTAACTCTCTAA
CCTTTCCTTTGCTTGTTTTGGGGTTTCAACGTGTTTTGTCTGATAGGAAAATGTTTGATGTTATTGACCTCATCTTGTTGATGGGGGAGTTTAGTCGTAGTCTTGGGAGA
AAGGATGTTCGTCTTTGGAGTCTTTATCCTTGTGAAAGCTTCTCTTGTAAATCCTTCTCCCTTTGCATGGTCAATCTCTCTCCCTTAGTTGGTGTCTTTGCCTCATAG

mRNA sequence

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Protein sequence

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MEAIEDDQSAKVDPPSSPSEPPLSKNARKKLLKQQRYEAKKAEKKAQAKELKKKDIERKRKEWEDTLASVTEEERSKLIESRRNLRKERMGKRSEEREKKIERLNRAKHS
GQNIVIDLEFSHLMTPSEIHSLVQQCLVGDGRDTFFWEDKWLGDRHLYSRFLHLHHLSSMKNHLVVDVLPNSLTFPLLVLGFQRVLSDRKMFDVIDLILLMGEFSRSLGR
KDVRLWSLYPCESFSCKSFSLCMVNLSPLVGVFAS

Spg019828 (gene) of Sponge gourd (cylindrica) v1 genome