CuGenDBv2

Gene ID	Spg021017
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Threonylcarbamoyladenosine tRNA methylthiotransferase
Genome location	scaffold9:1392984..1394922
RNA-Seq Expression	Spg021017
Synteny	Spg021017
Gene Ontology terms	GO:0035600 - tRNA methylthiolation (biological process) GO:0005783 - endoplasmic reticulum (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0035598 - N6-threonylcarbomyladenosine methylthiotransferase activity (molecular function) GO:0046872 - metal ion binding (molecular function) GO:0051539 - 4 iron, 4 sulfur cluster binding (molecular function) GO:0061712 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase (molecular function)
InterPro domains	NA

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

XP_022949069.1 threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita moschata]

3.1e-80

83.96

Show/hide

Query:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDD
        VGHTKGYIQVLVI  ETMLGTSA+VKITSVGRWSVFGEVIETI TKHHKTT LEDM I+ +VPPCS+AHE CACS+EP+ CACGPES CKGPVALD+KDD
Subjt:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDD

Query:  SSRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSS
        SSRNIPLPEE KRKNL+ WLLR+RK H PKREERESP+VSERKQA AG    +WGV+DRILVGGILISIFTIVGLLFHLGSTTLSSS
Subjt:  SSRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSS

XP_022997808.1 threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita maxima]

1.0e-78

82.89

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Query:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDD
        VGHTKGYIQVLVI  E MLGTSA+VKITSVGRWSVFGEVIETI TKHHKTT LEDM I+ +VPPCSN+HE CACS+EP+ CACGPES CKGPVALD+KDD
Subjt:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDD

Query:  SSRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSS
        SSRNIPLPEE KRKNL+ WLLR+RK HVPKREE ESP+VS RKQA AG    +WGV+DRILVGGILISIFTIVGLLFHLGSTTLSSS
Subjt:  SSRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSS

XP_023524910.1 threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita pepo subsp. pepo]

8.2e-81

84.49

Show/hide

Query:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDD
        VGHTKGYIQVLVI PE MLGTSA+VKITSVGRWSVFGEVIETI TKHHKTT LEDM I+ +VPPCSNAHE CACS+EP+ CACG ES CKGPVALD+KDD
Subjt:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDD

Query:  SSRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSS
        SSRNIPLPEE KRKNL+ WLLR+RK HVPKREERESP+VSERKQA AG    +WGV+DRILVGGILISIFTIVGLLFHLGSTTLSSS
Subjt:  SSRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSS

XP_038890156.1 threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Benincasa hispida]

7.2e-85

87.7

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Query:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDS
        VGHTKGYIQVLVIAPETMLGTSAMVKITS GRWSVFGEVIETISTKHHKTTTLED  IRD+VPPCSNAHE CACS+EP+ CACGPESCKG VA DDKDDS
Subjt:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDS

Query:  SRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSSW
        SRNIPLPEE KRKNL+ W LRRRK HVPKREERESPIVSERKQALAG    +WGVIDRILVGGILIS  TI GLLFHLGSTTLSSSW
Subjt:  SRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSSW

XP_038890157.1 threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2 [Benincasa hispida]

7.2e-85

87.7

Show/hide

Query:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDS
        VGHTKGYIQVLVIAPETMLGTSAMVKITS GRWSVFGEVIETISTKHHKTTTLED  IRD+VPPCSNAHE CACS+EP+ CACGPESCKG VA DDKDDS
Subjt:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDS

Query:  SRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSSW
        SRNIPLPEE KRKNL+ W LRRRK HVPKREERESPIVSERKQALAG    +WGVIDRILVGGILIS  TI GLLFHLGSTTLSSSW
Subjt:  SRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSSW

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KSZ4 Threonylcarbamoyladenosine tRNA methylthiotransferase

1.2e-74

78.19

Show/hide

Query:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDS
        VGHTKGYIQVLVIAPETMLGTSA VKITS+GRWSVFGEVIE ISTKHHKTTTLED L +D+V PCSN HE CACS EP+ CACG ESCKG VA+ D+ +S
Subjt:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDS

Query:  SRNIPLPEESKRKNLIGWLLRRRKGHV-PKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSSW
        SRN+P PEE KRKNLI W+LRRRK HV PKREE E+PIV+ERKQ LAG    +WGV+D+ILVGGILIS FTI GLLFHLGSTT SSSW
Subjt:  SRNIPLPEESKRKNLIGWLLRRRKGHV-PKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSSW

A0A1S3CA89 Threonylcarbamoyladenosine tRNA methylthiotransferase

3.4e-72

78.19

Show/hide

Query:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDS
        VGHTKGYIQVLVIAPETMLGTSAM KITS+GRWSVFGEVIETISTKHHK TTLED L RD+V PCSN HE CACS EP+ CAC   SCKG V++ D+ DS
Subjt:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDS

Query:  SRNIPLPEESKRKNLIGWLLRRRKGHV-PKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSSW
        SRNIPLPEE KRKNLI W+LRRRK  V PKREE E+PIV+ERKQ LAG    +WGV+D ILVGGILIS FTI GLLFH+GSTTLSSSW
Subjt:  SRNIPLPEESKRKNLIGWLLRRRKGHV-PKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSSW

A0A1S3CAU3 Threonylcarbamoyladenosine tRNA methylthiotransferase

3.4e-72

78.19

Show/hide

Query:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDS
        VGHTKGYIQVLVIAPETMLGTSAM KITS+GRWSVFGEVIETISTKHHK TTLED L RD+V PCSN HE CACS EP+ CAC   SCKG V++ D+ DS
Subjt:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDS

Query:  SRNIPLPEESKRKNLIGWLLRRRKGHV-PKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSSW
        SRNIPLPEE KRKNLI W+LRRRK  V PKREE E+PIV+ERKQ LAG    +WGV+D ILVGGILIS FTI GLLFH+GSTTLSSSW
Subjt:  SRNIPLPEESKRKNLIGWLLRRRKGHV-PKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSSW

A0A6J1GBS1 Threonylcarbamoyladenosine tRNA methylthiotransferase

1.5e-80

83.96

Show/hide

Query:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDD
        VGHTKGYIQVLVI  ETMLGTSA+VKITSVGRWSVFGEVIETI TKHHKTT LEDM I+ +VPPCS+AHE CACS+EP+ CACGPES CKGPVALD+KDD
Subjt:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDD

Query:  SSRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSS
        SSRNIPLPEE KRKNL+ WLLR+RK H PKREERESP+VSERKQA AG    +WGV+DRILVGGILISIFTIVGLLFHLGSTTLSSS
Subjt:  SSRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSS

A0A6J1KEZ5 Threonylcarbamoyladenosine tRNA methylthiotransferase

4.9e-79

82.89

Show/hide

Query:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDD
        VGHTKGYIQVLVI  E MLGTSA+VKITSVGRWSVFGEVIETI TKHHKTT LEDM I+ +VPPCSN+HE CACS+EP+ CACGPES CKGPVALD+KDD
Subjt:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPES-CKGPVALDDKDD

Query:  SSRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSS
        SSRNIPLPEE KRKNL+ WLLR+RK HVPKREE ESP+VS RKQA AG    +WGV+DRILVGGILISIFTIVGLLFHLGSTTLSSS
Subjt:  SSRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAG----DWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSS

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT1G72090.1 Methylthiotransferase

1.7e-23

40.78

Show/hide

Query:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDS
        VGHTKGY+QVLV  PE+MLGTSAM +ITSVGRWSVFGEVIET S+ + +T + E+  +     PCS+    C      + C C  ESC G     +  + 
Subjt:  VGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALDDKDDS

Query:  SRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAGDWGVIDRILVGGILISIFTIVGLLFHLGSTTL
        S NI   +++K K+              K+EE+E   V     ++A +WG ID+ LV G+ +S  TI+ LL  +    L
Subjt:  SRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAGDWGVIDRILVGGILISIFTIVGLLFHLGSTTL

Sequences

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CDS sequence

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ATGATTGAAGAAAATGTCAATGGCTGTGGATCTATGGTTGAGGAGGTTGGCCATACCAAGGGATACATACAGGTGCTAGTAATTGCCCCGGAAACTATGCTAGGAACTTC
TGCTATGGTGAAGATAACATCAGTTGGAAGGTGGTCCGTTTTTGGGGAAGTGATTGAAACCATCAGCACCAAACATCATAAAACAACTACTCTCGAAGACATGCTGATTC
GAGACGAAGTTCCTCCCTGCTCTAACGCTCACGAAATGTGTGCATGTTCCATGGAACCCAAACTTTGTGCTTGTGGACCAGAAAGCTGCAAAGGACCAGTTGCTCTGGAT
GATAAAGATGATTCCTCAAGGAATATCCCATTGCCCGAAGAGTCGAAACGGAAGAATCTGATTGGATGGCTATTGAGGAGACGGAAGGGCCATGTGCCCAAAAGGGAGGA
GAGAGAAAGTCCCATAGTATCTGAGAGAAAGCAGGCATTGGCTGGAGATTGGGGCGTCATCGATAGGATTCTTGTGGGAGGAATACTAATCAGTATCTTTACAATTGTTG
GTCTACTGTTTCATCTTGGATCCACCACACTCTCCTCCAGTTGGTGA

mRNA sequence

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Protein sequence

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MIEENVNGCGSMVEEVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGEVIETISTKHHKTTTLEDMLIRDEVPPCSNAHEMCACSMEPKLCACGPESCKGPVALD
DKDDSSRNIPLPEESKRKNLIGWLLRRRKGHVPKREERESPIVSERKQALAGDWGVIDRILVGGILISIFTIVGLLFHLGSTTLSSSW

Spg021017 (gene) of Sponge gourd (cylindrica) v1 genome