CuGenDBv2

Gene ID	Spg021180
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	SpoU_methylase domain-containing protein
Genome location	scaffold6:45518387..45520325
RNA-Seq Expression	Spg021180
Synteny	Spg021180
Gene Ontology terms	GO:0030488 - tRNA methylation (biological process) GO:0003723 - RNA binding (molecular function) GO:0016423 - tRNA (guanine) methyltransferase activity (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

XP_023005246.1 uncharacterized protein LOC111498319 isoform X2 [Cucurbita maxima]

3.0e-51

77.03

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Query:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF
        I  FI + GVNICKQKSDLE +RVKFLILIMSLVQELQLV+EKI+H N+K E+KDEFT  QPSD   YAEPTI IQK  NLFSSLQVELVSFA +SCSIF
Subjt:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF

Query:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL
        WS+VK DE ILPGSVKGKLGGPSQRRLPSSIAT VLLAV S    +S+
Subjt:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL

XP_038906253.1 uncharacterized protein LOC120092116 isoform X1 [Benincasa hispida]

2.7e-52

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Query:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF
        I  F+H+HGVNICKQKSDLE +RVKFLILIM+LVQELQLVQ+KI H NYKC++ DE T  QPSDNW YA  TIF QK ANL   LQVELVSFAT+SCSIF
Subjt:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF

Query:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL
        WS+VK DE +LPGSVKGKLGGPSQRRLPSS+ATLVLLAV S+   +S+
Subjt:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL

XP_038906254.1 uncharacterized protein LOC120092116 isoform X2 [Benincasa hispida]

2.7e-52

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Query:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF
        I  F+H+HGVNICKQKSDLE +RVKFLILIM+LVQELQLVQ+KI H NYKC++ DE T  QPSDNW YA  TIF QK ANL   LQVELVSFAT+SCSIF
Subjt:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF

Query:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL
        WS+VK DE +LPGSVKGKLGGPSQRRLPSS+ATLVLLAV S+   +S+
Subjt:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL

XP_038906255.1 uncharacterized protein LOC120092116 isoform X3 [Benincasa hispida]

2.7e-52

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Query:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF
        I  F+H+HGVNICKQKSDLE +RVKFLILIM+LVQELQLVQ+KI H NYKC++ DE T  QPSDNW YA  TIF QK ANL   LQVELVSFAT+SCSIF
Subjt:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF

Query:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL
        WS+VK DE +LPGSVKGKLGGPSQRRLPSS+ATLVLLAV S+   +S+
Subjt:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL

XP_038906256.1 uncharacterized protein LOC120092116 isoform X4 [Benincasa hispida]

2.7e-52

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Query:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF
        I  F+H+HGVNICKQKSDLE +RVKFLILIM+LVQELQLVQ+KI H NYKC++ DE T  QPSDNW YA  TIF QK ANL   LQVELVSFAT+SCSIF
Subjt:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF

Query:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL
        WS+VK DE +LPGSVKGKLGGPSQRRLPSS+ATLVLLAV S+   +S+
Subjt:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL

TrEMBL top hits

e value

%identity

Alignment

A0A6J1DPL7 uncharacterized protein LOC111022655 isoform X5

1.8e-49

77.3

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Query:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF
        I  FIH+HGVNI KQKSDLE +RVKFLILI+SLVQELQLVQEK    NYKCETKDE+T  QPSD+  YA PT FI+KF NLFSSL  ELVSFAT SCSIF
Subjt:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF

Query:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRS
        WS+VK DE  LP SVKGKLGGPSQRRLPS  ATLVLLAV S
Subjt:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRS

A0A6J1F2V7 uncharacterized protein LOC111441850 isoform X2

1.6e-50

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Query:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF
        I  FI + GVNICKQKSDLE +RVKFLILIMSLVQELQLV+EKI+H N+K E+ DEFT   PSD+  YAEPTI IQK  NLFSSLQ+ELVSFA +SCSIF
Subjt:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF

Query:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL
        WS+VK DE ILPGSVKGKLGGPSQRRLPSSIAT VLLAV S    +S+
Subjt:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL

A0A6J1F8L8 uncharacterized protein LOC111441850 isoform X1

1.6e-50

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Query:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF
        I  FI + GVNICKQKSDLE +RVKFLILIMSLVQELQLV+EKI+H N+K E+ DEFT   PSD+  YAEPTI IQK  NLFSSLQ+ELVSFA +SCSIF
Subjt:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF

Query:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL
        WS+VK DE ILPGSVKGKLGGPSQRRLPSSIAT VLLAV S    +S+
Subjt:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL

A0A6J1KSL3 uncharacterized protein LOC111498319 isoform X1

1.4e-51

77.03

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Query:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF
        I  FI + GVNICKQKSDLE +RVKFLILIMSLVQELQLV+EKI+H N+K E+KDEFT  QPSD   YAEPTI IQK  NLFSSLQVELVSFA +SCSIF
Subjt:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF

Query:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL
        WS+VK DE ILPGSVKGKLGGPSQRRLPSSIAT VLLAV S    +S+
Subjt:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL

A0A6J1KUF0 uncharacterized protein LOC111498319 isoform X2

1.4e-51

77.03

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Query:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF
        I  FI + GVNICKQKSDLE +RVKFLILIMSLVQELQLV+EKI+H N+K E+KDEFT  QPSD   YAEPTI IQK  NLFSSLQVELVSFA +SCSIF
Subjt:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF

Query:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL
        WS+VK DE ILPGSVKGKLGGPSQRRLPSSIAT VLLAV S    +S+
Subjt:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSL

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT4G17610.1 tRNA/rRNA methyltransferase (SpoU) family protein

3.2e-19

38.3

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Query:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF
        I +F+ ++G++  ++K+ L+    KFLI I+S++ ELQ +Q+ I+  +   ++K      + +      + +   +KFA +  S+  EL+ FA  SCSIF
Subjt:  IRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCYAEPTIFIQKFANLFSSLQVELVSFATMSCSIF

Query:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRS
        WS   ++   LPGSV GKLGGPSQRRL     T VL AV S
Subjt:  WSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRS

Sequences

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CDS sequence

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ATGGTTGGCCTCGGCCTCGGGCCAGGCCGAGGCCGACCATATTGGCCCGACCCTTTGGTCTGGTCTTCCTCTGGATCGATTTCCTGGTCCTATCTCTGCTCGGTTGTCCT
TGTCGGCTCCTTGTGCATCAGAGTGTTTATTCATGACCATGGTGTAAATATTTGCAAGCAGAAGAGTGATTTGGAGAGTGTACGTGTGAAGTTTCTAATACTTATCATGA
GCTTGGTTCAAGAACTTCAATTAGTTCAGGAGAAAATTGCTCACAGCAACTACAAATGTGAAACCAAGGATGAGTTTACCTTCTTGCAGCCAAGTGACAATTGGTGTTAT
GCAGAACCAACTATTTTTATCCAAAAATTTGCCAACCTTTTTTCGTCTCTACAGGTAGAGTTGGTTTCTTTTGCTACCATGTCTTGTTCCATATTCTGGTCCGACGTCAA
GTTGGACGAGGCAATATTACCAGGTTCTGTGAAAGGGAAACTTGGAGGCCCCAGTCAACGCCGATTACCATCCTCTATTGCTACTTTGGTTCTGCTAGCTGTACGATCTT
CAATGCTCTTTAGCTCTCTTACATTGTAG

mRNA sequence

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Protein sequence

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MVGLGLGPGRGRPYWPDPLVWSSSGSISWSYLCSVVLVGSLCIRVFIHDHGVNICKQKSDLESVRVKFLILIMSLVQELQLVQEKIAHSNYKCETKDEFTFLQPSDNWCY
AEPTIFIQKFANLFSSLQVELVSFATMSCSIFWSDVKLDEAILPGSVKGKLGGPSQRRLPSSIATLVLLAVRSSMLFSSLTL

Spg021180 (gene) of Sponge gourd (cylindrica) v1 genome