CuGenDBv2

Gene ID	Spg021965
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	HNH domain-containing protein
Genome location	scaffold2:4885539..4889055
RNA-Seq Expression	Spg021965
Synteny	Spg021965
Gene Ontology terms	GO:0090305 - nucleic acid phosphodiester bond hydrolysis (biological process) GO:0016020 - membrane (cellular component) GO:0003676 - nucleic acid binding (molecular function) GO:0004519 - endonuclease activity (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6587544.1 hypothetical protein SDJN03_16109, partial [Cucurbita argyrosperma subsp. sororia]

4.5e-89

85.5

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Query:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW
        MR SKESKKKTR SSPLRDRT  S RFESRR+ S+ SRLSPSTSLLDR+LVT EANLLGDLKLSPD  VSDYNPRSFP+SVKQQCWDKAEKIKGRDPDRW
Subjt:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW

Query:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
        RRDALGN VFRKLVGCPGCLCHDYDHILPYSK             ATVNRSKGNRTE+SRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
Subjt:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ

XP_022933556.1 uncharacterized protein LOC111440950 [Cucurbita moschata]

2.7e-89

85.5

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Query:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW
        MR SKESKKKTR SSPLRDRT  S RFESRR+ S+ SRLSPSTSLLDR+LVT EANLLGDLKLSPDD VSDYNPRSFP+SVKQQCWDKAEKIKGRDPDRW
Subjt:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW

Query:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
        RRDALGN VFRKLVGCPGCLCHDYDHILPYSK             ATVNRSKGNRTE+SRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQD GGCRIQ
Subjt:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ

XP_022972967.1 uncharacterized protein LOC111471489 [Cucurbita maxima]

3.7e-91

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Query:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW
        MR SKESKKKTR SSPLRDRT  STRFESRR+ SN SRLSPSTSLLDR+LVT EANLLGDLKLSPDD VSDYNPRSFP+SVKQQCWDKAEKIKGRDPDRW
Subjt:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW

Query:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
        RRDALGN VFRKLVGCPGCLCHDYDHILPYSK             ATVNRSKGNRTE+SRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
Subjt:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ

XP_023529236.1 uncharacterized protein LOC111792136 [Cucurbita pepo subsp. pepo]

9.2e-90

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Query:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW
        MR SKESKKKTR SSPLRDRT  STRFESRR+ S+ SRLSPSTSLLDR+LVT EANLLGDLKLSPD  VSDYNPRSFP+SVKQQCWDKAEKIKGRDPDRW
Subjt:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW

Query:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
        RRDALGN VFRKLVGCPGCLCHDYDHILPYSK             ATVNRSKGNRTE+SRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
Subjt:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ

XP_038880609.1 uncharacterized protein LOC120072255 [Benincasa hispida]

1.1e-90

86.5

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Query:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW
        MR +KESKKKTRSSSPLRDRTG  T  ESRRY SNPS LSPS SLLDR+L+TGEA+LLGDLKLSPDD VSDYNPRSFPHSVKQQCWDKAEKIKGRDP RW
Subjt:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW

Query:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
        RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK             ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
Subjt:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LUI5 HNH domain-containing protein

8.4e-89

84.5

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Query:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW
        MR SKESKKKTR SSPLRDRTG+ T  ESR Y S+PS LSPS SLLDR+LVTGE++LLGDLK SPDD VS+YNPRSFPHSVKQQCWDKAEKIKGRDPDRW
Subjt:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW

Query:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
        RRDALGN VFRKLVGCPGCLCHDYDHILPYSK             ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
Subjt:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ

A0A1S3BWU4 uncharacterized protein LOC103493997

2.9e-89

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Query:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW
        MR SKESKKKTR SSPLRDRTGA T  ES RY S+PS LSPS SLLDR+LVTGE+NLLGDLK SP+D VS+YNPRSFPHSVKQQCWDKAEKIKGRDPDRW
Subjt:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW

Query:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
        RRDALGN VFRKLVGCPGCLCHDYDHILPYSK             ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
Subjt:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ

A0A5A7US43 HNH endonuclease

2.9e-89

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Query:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW
        MR SKESKKKTR SSPLRDRTGA T  ES RY S+PS LSPS SLLDR+LVTGE+NLLGDLK SP+D VS+YNPRSFPHSVKQQCWDKAEKIKGRDPDRW
Subjt:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW

Query:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
        RRDALGN VFRKLVGCPGCLCHDYDHILPYSK             ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
Subjt:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ

A0A6J1EZE0 uncharacterized protein LOC111440950

1.3e-89

85.5

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Query:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW
        MR SKESKKKTR SSPLRDRT  S RFESRR+ S+ SRLSPSTSLLDR+LVT EANLLGDLKLSPDD VSDYNPRSFP+SVKQQCWDKAEKIKGRDPDRW
Subjt:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW

Query:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
        RRDALGN VFRKLVGCPGCLCHDYDHILPYSK             ATVNRSKGNRTE+SRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQD GGCRIQ
Subjt:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ

A0A6J1I688 uncharacterized protein LOC111471489

1.8e-91

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Query:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW
        MR SKESKKKTR SSPLRDRT  STRFESRR+ SN SRLSPSTSLLDR+LVT EANLLGDLKLSPDD VSDYNPRSFP+SVKQQCWDKAEKIKGRDPDRW
Subjt:  MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRW

Query:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
        RRDALGN VFRKLVGCPGCLCHDYDHILPYSK             ATVNRSKGNRTE+SRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ
Subjt:  RRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT1G18680.1 HNH endonuclease domain-containing protein

8.2e-20

35.2

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Query:  PRSFPHSVKQQCWDKAEKIKGRDPDRWRRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVS
        PR F    K +CW  A+ + GR P+RWR+D  GNIV ++   C GCLC +YDHI+PYSK               VNR K  +  +    L   S   + +
Subjt:  PRSFPHSVKQQCWDKAEKIKGRDPDRWRRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVS

Query:  GRDMDLLELSAYGNVHRGQDSGGCR
         +++D++E++ YG+V R      C+
Subjt:  GRDMDLLELSAYGNVHRGQDSGGCR

AT3G47490.1 HNH endonuclease

4.6e-55

56.72

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Query:  PSKESKKKTRSSSPLRDRTGASTRFESRRYA-SNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYN--PRSFPHSVKQQCWDKAEKIKGRDPDR
        P++   +   SSSP + R   S+   +R    +   ++  S +LLDRE          ++ L P     D N  PRSFP+SVKQQCW+KAEKIKGRDP+R
Subjt:  PSKESKKKTRSSSPLRDRTGASTRFESRRYA-SNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYN--PRSFPHSVKQQCWDKAEKIKGRDPDR

Query:  WRRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRI
        WRRD LGNIVFRKLVGCPGCLCHDYDHI+PYSK             A VNRSKGN+T+ISR+ELIQ+SSYCRV+GRDMDL+EL+AYGNV R   S GCRI
Subjt:  WRRDALGNIVFRKLVGCPGCLCHDYDHILPYSK-------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRI

Query:  Q
        Q
Subjt:  Q

AT3G47490.2 HNH endonuclease

9.3e-32

54.14

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Query:  PSKESKKKTRSSSPLRDRTGASTRFESRRYA-SNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYN--PRSFPHSVKQQCWDKAEKIKGRDPDR
        P++   +   SSSP + R   S+   +R    +   ++  S +LLDRE          ++ L P     D N  PRSFP+SVKQQCW+KAEKIKGRDP+R
Subjt:  PSKESKKKTRSSSPLRDRTGASTRFESRRYA-SNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYN--PRSFPHSVKQQCWDKAEKIKGRDPDR

Query:  WRRDALGNIVFRKLVGCPGCLCHDYDHILPYSK
        WRRD LGNIVFRKLVGCPGCLCHDYDHI+PYSK
Subjt:  WRRDALGNIVFRKLVGCPGCLCHDYDHILPYSK

AT3G47490.3 HNH endonuclease

9.5e-53

51.58

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Query:  PSKESKKKTRSSSPLRDRTGASTRFESRRYA-SNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYN--PRSFPHSVKQQCWDKAEKIKGRDPDR
        P++   +   SSSP + R   S+   +R    +   ++  S +LLDRE          ++ L P     D N  PRSFP+SVKQQCW+KAEKIKGRDP+R
Subjt:  PSKESKKKTRSSSPLRDRTGASTRFESRRYA-SNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYN--PRSFPHSVKQQCWDKAEKIKGRDPDR

Query:  WRRDALGNIVFRKLVGCPGCLCHDYDHILPYSK---------------------------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDL
        WRRD LGNIVFRKLVGCPGCLCHDYDHI+PYSK                                 A VNRSKGN+T+ISR+ELIQ+SSYCRV+GRDMDL
Subjt:  WRRDALGNIVFRKLVGCPGCLCHDYDHILPYSK---------------------------------ATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDL

Query:  LELSAYGNVHRGQDSGGCRIQ
        +EL+AYGNV R   S GCRIQ
Subjt:  LELSAYGNVHRGQDSGGCRIQ

Sequences

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CDS sequence

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ATGAGGCCTAGCAAAGAATCGAAGAAGAAAACTCGTTCCTCATCACCGCTGAGGGACCGAACCGGTGCTTCAACGAGGTTCGAATCGAGGCGTTATGCGTCTAACCCATC
AAGGCTCAGCCCCTCTACTTCCCTTCTCGACCGTGAGCTCGTAACCGGCGAAGCCAATCTCCTCGGAGACCTAAAACTCTCTCCCGATGACCCAGTTTCCGACTATAATC
CCCGGAGCTTTCCTCACAGTGTGAAGCAGCAATGTTGGGACAAGGCCGAGAAGATTAAAGGTAGAGACCCAGATCGGTGGCGCCGTGATGCTCTCGGCAACATCGTTTTT
CGAAAGCTTGTTGGCTGCCCAGGTTGTCTTTGTCATGATTATGATCACATTTTGCCTTACTCTAAGGCAACCGTTAATCGATCAAAGGGAAACCGTACTGAAATTTCAAG
AGCAGAACTAATACAGAAAAGCTCTTACTGCCGGGTTTCAGGTCGAGACATGGATCTTCTTGAACTGTCTGCCTATGGCAATGTACACCGTGGACAAGACTCAGGAGGGT
GTAGAATTCAGTGA

mRNA sequence

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Protein sequence

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MRPSKESKKKTRSSSPLRDRTGASTRFESRRYASNPSRLSPSTSLLDRELVTGEANLLGDLKLSPDDPVSDYNPRSFPHSVKQQCWDKAEKIKGRDPDRWRRDALGNIVF
RKLVGCPGCLCHDYDHILPYSKATVNRSKGNRTEISRAELIQKSSYCRVSGRDMDLLELSAYGNVHRGQDSGGCRIQ

Spg021965 (gene) of Sponge gourd (cylindrica) v1 genome