CuGenDBv2

Gene ID	Spg023287
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RNase H domain-containing protein
Genome location	scaffold13:7456515..7457130
RNA-Seq Expression	Spg023287
Synteny	Spg023287
Gene Ontology terms	GO:0044237 - cellular metabolic process (biological process) GO:0003676 - nucleic acid binding (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function) GO:0016740 - transferase activity (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily IPR044730 - Ribonuclease H-like domain, plant type

Homology

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GenBank top hits

e value

%identity

Alignment

EMS54402.1 Ketohexokinase [Triticum urartu]

5.5e-09

36.36

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Query:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVAD
        V RD +GD + A + F+         EL AI  GI LA  +GCN I IESDC+ A+D +++     G   A +      +  F+  SF H  ++ N VAD
Subjt:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVAD

Query:  KIAKRARMLNSSDIWVFSISDWIVSLVDSDCS
        K+AK +    SS  W   I D+I  L+ +D S
Subjt:  KIAKRARMLNSSDIWVFSISDWIVSLVDSDCS

VAH20608.1 unnamed protein product [Triticum turgidum subsp. durum]

2.5e-09

33.33

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Query:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELET--IWSDVASFSDCSFIHTPKKKNLV
        V RD +GD + A + F+     A   E+ AI  G+ LA  +GCN +I+ESD + A+D+++++     E+   +E   + SD   F+    IH  ++ N V
Subjt:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELET--IWSDVASFSDCSFIHTPKKKNLV

Query:  ADKIAKRARMLNSSDIWVFSISDWIVSLVDSD
        AD IAK A    SS +W   + D+I   + +D
Subjt:  ADKIAKRARMLNSSDIWVFSISDWIVSLVDSD

VAH65567.1 unnamed protein product [Triticum turgidum subsp. durum]

1.2e-08

34.85

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Query:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVAD
        V RD +GD + A + F+         EL AI  GI LA  +GCN I IESDC+ A++ +++     G   A +      +  F+  SF H  ++ N VAD
Subjt:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVAD

Query:  KIAKRARMLNSSDIWVFSISDWIVSLVDSDCS
        ++AK      SS  W   I D+I  L+ +D S
Subjt:  KIAKRARMLNSSDIWVFSISDWIVSLVDSDCS

XP_023915006.1 uncharacterized protein LOC112026546 [Quercus suber]

8.5e-10

29.61

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Query:  FSKANLREAPRE---VSSPRLVCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSD
        F+K N+  A  +    SS  ++ RDS+G ++ A    L + F A + E  A+ +G+ LA  LG +K+I ESD    I  +++ +   GE+   ++ I   
Subjt:  FSKANLREAPRE---VSSPRLVCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSD

Query:  VASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSISDWIVSLVDSDC
         +SFS C+F H  ++ N  A ++A+ ARM   S +W      ++  ++  +C
Subjt:  VASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSISDWIVSLVDSDC

XP_038902513.1 uncharacterized protein LOC120089172 [Benincasa hispida]

5.0e-10

33.33

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Query:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVAD
        + RD++G     +   +DA +  PL E   + +G++L   +   KII++SDC+ AIDL  K       +   LE IW     F   +F + P+  N +AD
Subjt:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVAD

Query:  KIAKRARMLNSSDIWVFSIS
         +AKR R+L  +D+W+ S S
Subjt:  KIAKRARMLNSSDIWVFSIS

TrEMBL top hits

e value

%identity

Alignment

A0A446K5I3 RNase H domain-containing protein

1.2e-09

33.33

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Query:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELET--IWSDVASFSDCSFIHTPKKKNLV
        V RD +GD + A + F+     A   E+ AI  G+ LA  +GCN +I+ESD + A+D+++++     E+   +E   + SD   F+    IH  ++ N V
Subjt:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELET--IWSDVASFSDCSFIHTPKKKNLV

Query:  ADKIAKRARMLNSSDIWVFSISDWIVSLVDSD
        AD IAK A    SS +W   + D+I   + +D
Subjt:  ADKIAKRARMLNSSDIWVFSISDWIVSLVDSD

A0A446NU09 RNase H domain-containing protein

6.0e-09

34.85

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Query:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVAD
        V RD +GD + A + F+         EL AI  GI LA  +GCN I IESDC+ A++ +++     G   A +      +  F+  SF H  ++ N VAD
Subjt:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVAD

Query:  KIAKRARMLNSSDIWVFSISDWIVSLVDSDCS
        ++AK      SS  W   I D+I  L+ +D S
Subjt:  KIAKRARMLNSSDIWVFSISDWIVSLVDSDCS

A0A446W4K1 RNase H domain-containing protein

1.9e-07

28.79

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Query:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVAD
        + RD +G+ + A   F+     A   E++AI  G  LA+ +GCN +I+ESD   A+    ++    G     +         F+  S++H  ++ N VAD
Subjt:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVAD

Query:  KIAKRARMLNSSDIWVFSISDWIVSLVDSDCS
         +AK + +  SS +W  S  D+I   + +D S
Subjt:  KIAKRARMLNSSDIWVFSISDWIVSLVDSDCS

M7Z366 Ketohexokinase

2.7e-09

36.36

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Query:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVAD
        V RD +GD + A + F+         EL AI  GI LA  +GCN I IESDC+ A+D +++     G   A +      +  F+  SF H  ++ N VAD
Subjt:  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVAD

Query:  KIAKRARMLNSSDIWVFSISDWIVSLVDSDCS
        K+AK +    SS  W   I D+I  L+ +D S
Subjt:  KIAKRARMLNSSDIWVFSISDWIVSLVDSDCS

M8AHN8 Zinc finger CCCH domain-containing protein 7

1.6e-09

33.55

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Query:  EYLEEFSKANLREAPREVSSPRLVCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKS--APWGELEAELET
        E + EF  A L  A      P   CRD +G+ +GA + F+     A   E+ AI  G+ LA  LGCN +I+ESD   AI+  ++     P   + AE +T
Subjt:  EYLEEFSKANLREAPREVSSPRLVCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKS--APWGELEAELET

Query:  IWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSISDWIVSLVDSD
        I  D+A F      H  ++ N VAD IAK +   +S++ W   I D++   +  D
Subjt:  IWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSISDWIVSLVDSD

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT1G52990.1 thioredoxin family protein

4.4e-04

32.58

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Query:  ELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIW
        E  A+   I+     G  K+I E D +    L++ KS     L+  L+TI S + SF+   FI T +++N  AD + K+A  + SS  W
Subjt:  ELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIW

AT2G34320.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

1.1e-04

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Query:  ELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSI-SDWIVS
        EL+A+   +         +II ESD    ++L++     W  L+  LE I   +  F +  F  TP+  N VAD+IA+ +   ++ D  +FSI   W+ S
Subjt:  ELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSI-SDWIVS

Sequences

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CDS sequence

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ATGATCAGAAACTCTTGGATAAAAGAATACTTAGAAGAATTTTCGAAAGCAAACCTGAGAGAAGCTCCAAGAGAAGTTTCGAGCCCTCGCCTTGTTTGCAGAGACTCAAA
AGGCGATGCCCTAGGAGCGAAAACCATTTTTCTTGATGCGGACTTTAGGGCTCCTTTGATAGAATTGAAAGCTATCGGAGAAGGAATCAAGCTGGCAAAACTATTGGGTT
GCAATAAAATCATAATTGAATCTGACTGTACTCAAGCCATTGACCTTGTATCTAAAAAGTCAGCCCCCTGGGGTGAGTTGGAAGCAGAGCTGGAGACCATATGGTCAGAT
GTGGCAAGTTTTTCTGATTGTTCCTTTATTCATACTCCTAAGAAGAAGAATTTAGTGGCAGATAAAATAGCTAAAAGAGCGAGAATGTTAAATTCGAGTGATATCTGGGT
TTTCTCTATCTCGGATTGGATTGTATCTTTGGTCGATTCGGACTGTTCATCGTTTGTCCAAGTGGCGCCTTAA

mRNA sequence

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Protein sequence

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MIRNSWIKEYLEEFSKANLREAPREVSSPRLVCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSD
VASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSISDWIVSLVDSDCSSFVQVAP

Spg023287 (gene) of Sponge gourd (cylindrica) v1 genome