CuGenDBv2

Gene ID	Spg023860
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Ent-kaurene oxidase
Genome location	scaffold13:6237480..6238272
RNA-Seq Expression	Spg023860
Synteny	Spg023860
Gene Ontology terms	GO:0009686 - gibberellin biosynthetic process (biological process) GO:0010241 - ent-kaurene oxidation to kaurenoic acid (biological process) GO:0009707 - chloroplast outer membrane (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0005506 - iron ion binding (molecular function) GO:0020037 - heme binding (molecular function) GO:0052615 - ent-kaurene oxidase activity (molecular function)
InterPro domains	IPR044225 - Ent-kaurene oxidase, chloroplastic

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6608504.1 Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]

5.9e-18

94.34

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

KAG7037828.1 Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma]

5.9e-18

94.34

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

XP_022941414.1 ent-kaurene oxidase, chloroplastic-like [Cucurbita moschata]

5.9e-18

94.34

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

XP_022982224.1 ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima]

5.9e-18

94.34

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

XP_023524792.1 ent-kaurene oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo]

5.9e-18

94.34

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

TrEMBL top hits

e value

%identity

Alignment

A0A6J1CGM6 ent-kaurene oxidase, chloroplastic

6.4e-18

92.45

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHK+ KRYILAN+LG NAQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

A0A6J1FN94 ent-kaurene oxidase, chloroplastic-like

2.9e-18

94.34

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

A0A6J1J3Y4 ent-kaurene oxidase, chloroplastic-like

2.9e-18

94.34

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

D5LXQ4 Ent-kaurene oxidase

6.4e-18

92.45

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHK+ KRYILAN+LG NAQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

Q9FQY5 Ent-kaurene oxidase

2.9e-18

94.34

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

SwissProt top hits

e value

%identity

Alignment

A0A0G2RKY1 Ent-kaurene oxidase

1.6e-13

69.81

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        M+T+YSSIS+RKLSKALTILTSDK +VAMSDYN+F+K  KR++L + LG  AQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

C7J3A2 Ent-kaurene oxidase-like protein 1

7.0e-14

67.92

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MV ++SSIS+RKLSKALT+LT DK MVA SDY DFHK+VKRY++++ LG +AQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

Q5Z5R4 Ent-kaurene oxidase 2

7.0e-14

67.92

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MV ++SSIS+RKLSKALT+LT DK MVA SDY DFHK+VKRY+++++LG +AQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

Q5Z5R7 Ent-kaurene oxidase-like 3

8.6e-12

64.15

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MV ++SSIS+RKLSKALT+L+ DK MVA SD  DFHK+ KRYI+ ++LG +AQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

Q93ZB2 Ent-kaurene oxidase, chloroplastic

2.4e-14

75.47

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MVTR+SSIS+RKLS ALT+LT DK MVA SDY+DFHKLVKR +L  LLG NAQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

Arabidopsis top hits

e value

%identity

Alignment

AT5G25900.1 GA requiring 3

1.7e-15

75.47

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Query:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ
        MVTR+SSIS+RKLS ALT+LT DK MVA SDY+DFHKLVKR +L  LLG NAQ
Subjt:  MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

Sequences

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CDS sequence

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ATGGTGACGAGATATTCCTCCATTTCCAGTAGAAAGCTAAGCAAAGCCCTCACCATTTTGACTTCTGATAAATGCATGGTTGCTATGAGTGATTACAATGACTTTCACAA
GTTGGTGAAGAGATATATATTGGCTAATCTCTTGGGTGTTAATGCTCAG

mRNA sequence

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ATGGTGACGAGATATTCCTCCATTTCCAGTAGAAAGCTAAGCAAAGCCCTCACCATTTTGACTTCTGATAAATGCATGGTTGCTATGAGTGATTACAATGACTTTCACAA
GTTGGTGAAGAGATATATATTGGCTAATCTCTTGGGTGTTAATGCTCAG

Protein sequence

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MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQ

Spg023860 (gene) of Sponge gourd (cylindrica) v1 genome