CuGenDBv2

Gene ID	Spg024421
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Quinolinate synthase
Genome location	scaffold9:24162939..24163605
RNA-Seq Expression	Spg024421
Synteny	Spg024421
Gene Ontology terms	GO:0019805 - quinolinate biosynthetic process (biological process) GO:0034628 - 'de novo' NAD biosynthetic process from aspartate (biological process) GO:0009507 - chloroplast (cellular component) GO:0008987 - quinolinate synthetase A activity (molecular function) GO:0046872 - metal ion binding (molecular function) GO:0051539 - 4 iron, 4 sulfur cluster binding (molecular function)
InterPro domains	IPR003808 - Fe-S metabolism associated domain, SufE-like

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6584113.1 Quinolinate synthase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]

1.2e-24

84.51

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Query:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR
        MGC   VWLEVRIDQE KMRF+ADS+SEISKGFCSCLVSVLDGA+PEDVLRLK D+LAALNV L GGERSR
Subjt:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR

XP_004152496.1 quinolinate synthase, chloroplastic [Cucumis sativus]

2.0e-24

83.1

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Query:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR
        MGC   VWLEVRIDQE KMRF+ADS+SEISKGFCSCLVSVLDGA+PEDVLRLK ++LAALNV L GGERSR
Subjt:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR

XP_022923855.1 quinolinate synthase, chloroplastic [Cucurbita moschata]

1.2e-24

84.51

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Query:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR
        MGC   VWLEVRIDQE KMRF+ADS+SEISKGFCSCLVSVLDGA+PEDVLRLK D+LAALNV L GGERSR
Subjt:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR

XP_023001233.1 quinolinate synthase, chloroplastic [Cucurbita maxima]

2.7e-24

83.1

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Query:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR
        MGC   VWLEVRIDQE KMRF+ADS+SEISKGFCSCLVSVLDGA+PEDVL+LK D+LAALNV L GGERSR
Subjt:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR

XP_023520087.1 quinolinate synthase, chloroplastic [Cucurbita pepo subsp. pepo]

1.2e-24

84.51

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Query:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR
        MGC   VWLEVRIDQE KMRF+ADS+SEISKGFCSCLVSVLDGA+PEDVLRLK D+LAALNV L GGERSR
Subjt:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LRC5 Quinolinate synthase

9.9e-25

83.1

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Query:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR
        MGC   VWLEVRIDQE KMRF+ADS+SEISKGFCSCLVSVLDGA+PEDVLRLK ++LAALNV L GGERSR
Subjt:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR

A0A1S3AXE7 Quinolinate synthase

4.9e-24

80.28

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Query:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR
        MGC   VWLEVRIDQE KMRF+ADS+SEIS+GFCSCLVSVLDGA+PEDVLRLK ++L+ALNV L GGERSR
Subjt:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR

A0A5A7UJT0 Quinolinate synthase

4.9e-24

80.28

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Query:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR
        MGC   VWLEVRIDQE KMRF+ADS+SEIS+GFCSCLVSVLDGA+PEDVLRLK ++L+ALNV L GGERSR
Subjt:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR

A0A6J1ED41 Quinolinate synthase

5.8e-25

84.51

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Query:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR
        MGC   VWLEVRIDQE KMRF+ADS+SEISKGFCSCLVSVLDGA+PEDVLRLK D+LAALNV L GGERSR
Subjt:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR

A0A6J1KPX9 Quinolinate synthase

1.3e-24

83.1

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Query:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR
        MGC   VWLEVRIDQE KMRF+ADS+SEISKGFCSCLVSVLDGA+PEDVL+LK D+LAALNV L GGERSR
Subjt:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR

SwissProt top hits

e value

%identity

Alignment

Q2QTL0 Quinolinate synthase, chloroplastic

6.0e-11

35.62

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Query:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLG-GERSRWPKKPIRVAVNRLHARKNRRRREALPK-
        MGC   VWL  R D   +MRF+ADS+SE+S+G+C+CLVS LDGA PE+VL +   +LA L     G G RSR       +   +  AR     RE  P  
Subjt:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLG-GERSRWPKKPIRVAVNRLHARKNRRRREALPK-

Query:  EPHADVLLISTNVQILERSFSRSFTRINPAEESKNEEEKKEKERKR
        EP   +++        + +++ +        ESK  E  K    K+
Subjt:  EPHADVLLISTNVQILERSFSRSFTRINPAEESKNEEEKKEKERKR

Q9FGS4 Quinolinate synthase, chloroplastic

2.7e-19

41.73

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Query:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR---WPKKPIRVAVNRLHARKNRRRREALP
        MGC   VWL+  + Q+ KMRF ADS+S++SKG CSCL+ VLD A P +V+ LK ++LA LNV LLGGERSR   W    + V++ +   R    R   +P
Subjt:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR---WPKKPIRVAVNRLHARKNRRRREALP

Query:  K-EPHADVLLISTNVQILERSFSRSFTRINPAEESKNEE
          EP   ++L +  ++  + SF+++  +    EES+ EE
Subjt:  K-EPHADVLLISTNVQILERSFSRSFTRINPAEESKNEE

Q9FXE3 SufE-like protein 2, chloroplastic

6.6e-18

55.71

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Query:  GCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR
        GC T VWLE+++D+  +MRF ADS+SEISKGFCSCL+ +LDGA PE+V+ ++ ++L+ +NV + G E+SR
Subjt:  GCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR

Arabidopsis top hits

e value

%identity

Alignment

AT1G67810.1 sulfur E2

4.7e-19

55.71

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Query:  GCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR
        GC T VWLE+++D+  +MRF ADS+SEISKGFCSCL+ +LDGA PE+V+ ++ ++L+ +NV + G E+SR
Subjt:  GCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR

AT4G26500.1 chloroplast sulfur E

1.5e-04

32.43

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Query:  GCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSRWPKK
        GC + VW+    D+E  + + ADS+S ++KG  + LV  L G    ++LR+  D        LLG ++S  P +
Subjt:  GCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSRWPKK

AT5G50210.1 quinolinate synthase

1.9e-20

41.73

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Query:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR---WPKKPIRVAVNRLHARKNRRRREALP
        MGC   VWL+  + Q+ KMRF ADS+S++SKG CSCL+ VLD A P +V+ LK ++LA LNV LLGGERSR   W    + V++ +   R    R   +P
Subjt:  MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSR---WPKKPIRVAVNRLHARKNRRRREALP

Query:  K-EPHADVLLISTNVQILERSFSRSFTRINPAEESKNEE
          EP   ++L +  ++  + SF+++  +    EES+ EE
Subjt:  K-EPHADVLLISTNVQILERSFSRSFTRINPAEESKNEE

Sequences

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CDS sequence

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ATGGGGTGTAAGACGCATGTGTGGTTGGAGGTGAGGATTGATCAAGAGGACAAGATGAGGTTCTCTGCGGATAGTGAATCGGAGATCTCTAAAGGGTTTTGCTCGTGTTT
AGTTTCGGTGCTTGATGGTGCGTTGCCAGAGGATGTTCTGAGGTTGAAAATGGACAATTTGGCTGCTCTGAATGTGGATTTGCTTGGTGGAGAGCGATCGAGATGGCCGA
AGAAACCCATTCGCGTCGCCGTCAATCGCCTGCACGCACGAAAAAATCGACGACGGCGTGAAGCTTTGCCTAAAGAGCCCCACGCCGATGTTCTTCTCATCAGTACAAAC
GTCCAGATCCTTGAACGAAGCTTTTCCCGAAGCTTCACCCGAATAAATCCAGCTGAAGAGAGTAAAAATGAAGAAGAAAAGAAGGAGAAAGAAAGAAAAAGAAAAAGAAA
AAAATCGTCGGCGGCGATCGTCAGAGGTCGGAGTTGCAACAACAGTAGTATCAGGTGGTGA

mRNA sequence

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Protein sequence

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MGCKTHVWLEVRIDQEDKMRFSADSESEISKGFCSCLVSVLDGALPEDVLRLKMDNLAALNVDLLGGERSRWPKKPIRVAVNRLHARKNRRRREALPKEPHADVLLISTN
VQILERSFSRSFTRINPAEESKNEEEKKEKERKRKRKKSSAAIVRGRSCNNSSIRW

Spg024421 (gene) of Sponge gourd (cylindrica) v1 genome