CuGenDBv2

Gene ID	Spg025588
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Histidine--tRNA ligase
Genome location	scaffold13:30147208..30148853
RNA-Seq Expression	Spg025588
Synteny	Spg025588
Gene Ontology terms	GO:0006427 - histidyl-tRNA aminoacylation (biological process) GO:0005737 - cytoplasm (cellular component) GO:0004821 - histidine-tRNA ligase activity (molecular function) GO:0005524 - ATP binding (molecular function)
InterPro domains	IPR004516 - Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunit

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6604395.1 Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia]

3.6e-43

74.63

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Query:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG
        A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK        M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGG
Subjt:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG

Query:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY
        EA+SDLKQLFSLAEKFGY DW+QFDA IV GLAY
Subjt:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY

KAG7034545.1 Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma]

1.2e-43

65.62

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Query:  LIQESLWQLGVVIPTIKHL-TENGAKVLLSSHSISVL--LGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSE
        L+ E L ++     ++ H+  ++GA+  L S  ++    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK        M KELKVVGVSE
Subjt:  LIQESLWQLGVVIPTIKHL-TENGAKVLLSSHSISVL--LGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSE

Query:  EAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY
        +AIEE+L+VLSVK LTKLEEILGGGGEA+SDLKQLFSLAEKFGY DW+QFDA IV GLAY
Subjt:  EAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY

XP_022978407.1 histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima]

3.6e-43

74.63

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Query:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG
        A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK        M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGG
Subjt:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG

Query:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY
        EA+SDLKQLFSLAEKFGY DW+QFDA IV GLAY
Subjt:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY

XP_023544232.1 histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo subsp. pepo]

3.6e-43

74.63

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Query:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG
        A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK        M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGG
Subjt:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG

Query:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY
        EA+SDLKQLFSLAEKFGY DW+QFDA IV GLAY
Subjt:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY

XP_023544233.1 histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo subsp. pepo]

3.6e-43

74.63

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Query:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG
        A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK        M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGG
Subjt:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG

Query:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY
        EA+SDLKQLFSLAEKFGY DW+QFDA IV GLAY
Subjt:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY

TrEMBL top hits

e value

%identity

Alignment

A0A5D3C5Z0 Histidine--tRNA ligase

8.6e-43

73.13

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Query:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG
        A++L S  +    +G+ + DVGFKVSSRKVLQ+VLKCYGIP+N+FGKVCII+DK        M KELKVVGVSE+AIEELL+VLSVK LTKLEE+LGGGG
Subjt:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG

Query:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY
        EALSDLKQLFSLAEKFGY DW+QFDA IV GLAY
Subjt:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY

A0A6J1ECF7 Histidine--tRNA ligase

1.7e-43

74.63

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Query:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG
        A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK        M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGG
Subjt:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG

Query:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY
        EA+SDLKQLFSLAEKFGY DW+QFDA IV GLAY
Subjt:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY

A0A6J1EG18 Histidine--tRNA ligase

1.7e-43

74.63

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Query:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG
        A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK        M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGG
Subjt:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG

Query:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY
        EA+SDLKQLFSLAEKFGY DW+QFDA IV GLAY
Subjt:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY

A0A6J1IL10 Histidine--tRNA ligase

1.7e-43

74.63

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Query:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG
        A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK        M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGG
Subjt:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG

Query:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY
        EA+SDLKQLFSLAEKFGY DW+QFDA IV GLAY
Subjt:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY

A0A6J1IMK6 Histidine--tRNA ligase

1.7e-43

74.63

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Query:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG
        A++L S  +    +GL + DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCII+DK        M KELKVVGVSE+AIEE+L+VLSVK LTKLEEILGGGG
Subjt:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGG

Query:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY
        EA+SDLKQLFSLAEKFGY DW+QFDA IV GLAY
Subjt:  EALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY

SwissProt top hits

e value

%identity

Alignment

O82413 Histidine--tRNA ligase, chloroplastic/mitochondrial

4.0e-37

65.29

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Query:  LGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLA
        +G+ + DVGFKVSSRKVLQE+LK YG+PE+LFG+VCII+DK        + KEL   G+SE+AIE+LL+VLSVK L  LE+I+GG GEA++DLKQLFSLA
Subjt:  LGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLA

Query:  EKFGYVDWVQFDAFIVCGLAY
        EKFGY +W+QFDA +V GLAY
Subjt:  EKFGYVDWVQFDAFIVCGLAY

P70076 Histidine--tRNA ligase, cytoplasmic

5.8e-04

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Query:  DVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDKMTK---------ELKVVGVSEEAIEELLKVLSVKFLTKLEEIL---------GGGGEALSDLKQL
        D   KV+ R++L  +    G+P+N+F  +C  +DK+ K          +   G+SEEA +++   + ++    L E L               L+D+K L
Subjt:  DVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDKMTK---------ELKVVGVSEEAIEELLKVLSVKFLTKLEEIL---------GGGGEALSDLKQL

Query:  FSLAEKFGYVDWVQFDAFIVCGLAY
        FS  + F   D V FD  +  GL Y
Subjt:  FSLAEKFGYVDWVQFDAFIVCGLAY

Arabidopsis top hits

e value

%identity

Alignment

AT3G46100.1 Histidyl-tRNA synthetase 1

2.8e-38

65.29

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Query:  LGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLA
        +G+ + DVGFKVSSRKVLQE+LK YG+PE+LFG+VCII+DK        + KEL   G+SE+AIE+LL+VLSVK L  LE+I+GG GEA++DLKQLFSLA
Subjt:  LGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDK--------MTKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLA

Query:  EKFGYVDWVQFDAFIVCGLAY
        EKFGY +W+QFDA +V GLAY
Subjt:  EKFGYVDWVQFDAFIVCGLAY

AT5G03406.1 Class II aaRS and biotin synthetases superfamily protein

2.3e-11

55.36

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Query:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDKMT
        A+++ S  +    +G+ + DVGFKVSSRKVLQE LK YG+PE+LFG+VC I+DK++
Subjt:  AKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDKMT

Sequences

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CDS sequence

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ATGCTTTATTTATTTCAGAAAGTGCAATGGCTCCTTATTTATGAAGCTTCAAGGGATTGGTTGGTGTTGCTTCAGTTGAATTTATTGATCCAAGAGAGCTTGTGGCAGTT
AGGTGTTGTTATTCCTACGATCAAGCATTTGACTGAGAATGGAGCTAAAGTGCTTCTGTCTAGCCATTCGATAAGTGTGCTTTTGGGACTTCTTTCCCCAGATGTTGGAT
TCAAGGTTTCCAGTCGGAAGGTTTTGCAAGAAGTATTAAAATGCTATGGAATACCTGAAAATCTGTTTGGCAAGGTTTGCATCATCTTAGACAAGATGACGAAGGAATTG
AAGGTCGTGGGTGTATCAGAGGAGGCTATTGAGGAGCTGCTGAAAGTTCTTTCAGTAAAGTTTTTGACAAAGTTAGAAGAAATACTTGGAGGTGGTGGCGAAGCGTTGTC
TGATCTAAAGCAGCTATTTTCTCTTGCTGAAAAGTTTGGGTATGTTGATTGGGTCCAATTTGATGCATTCATTGTCTGTGGCCTTGCTTATTAA

mRNA sequence

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Protein sequence

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MLYLFQKVQWLLIYEASRDWLVLLQLNLLIQESLWQLGVVIPTIKHLTENGAKVLLSSHSISVLLGLLSPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIILDKMTKEL
KVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLAEKFGYVDWVQFDAFIVCGLAY

Spg025588 (gene) of Sponge gourd (cylindrica) v1 genome