CuGenDBv2

Gene ID	Spg026416
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	tRNA:m(4)X modification enzyme TRM13
Genome location	scaffold13:18189648..18194249
RNA-Seq Expression	Spg026416
Synteny	Spg026416
Gene Ontology terms	GO:0008033 - tRNA processing (biological process) GO:0008168 - methyltransferase activity (molecular function)
InterPro domains	IPR007871 - Methyltransferase TRM13

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6575728.1 tRNA:m(4)X modification enzyme TRM13, partial [Cucurbita argyrosperma subsp. sororia]

3.7e-53

50.2

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Query:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG
        +SS+E   GL D ISSEM+RNI+YGM GAEFHRLL KI+ALHGLICKDIQDSYRIKEACNMWIKGEID KIPFQE                    NYDGG
Subjt:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG

Query:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------
        EQCEGNRS GNEANV                                          L +KESL++E+    +                           
Subjt:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------

Query:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS
         DLALRCCLTKQSNHV+ E C DRPKL GLA  T CHHLCQWKHYT+
Subjt:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS

KAG7014284.1 tRNA:m(4)X modification enzyme TRM13-like protein [Cucurbita argyrosperma subsp. argyrosperma]

9.7e-54

50.61

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Query:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG
        +SSDE   GL D ISSEM+RNI+YGM GAEFHRLL KI+ALHGLICKDIQDSYRIKEACNMWIKGEID KIPFQE                    NYDGG
Subjt:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG

Query:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------
        EQCEGNRS GNEANV                                          L +KESL++E+    +                           
Subjt:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------

Query:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS
         DLALRCCLTKQSNHV+ E C DRPKL GLA  T CHHLCQWKHYT+
Subjt:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS

XP_022954384.1 tRNA:m(4)X modification enzyme TRM13 homolog [Cucurbita moschata]

3.3e-54

51.01

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Query:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG
        +SSDE   GL DSISSEM+RNI+YGM GAEFHRLL KI+ALHGLICKDIQDSYRIKEACNMWIKGEID KIPFQE                    NYDGG
Subjt:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG

Query:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------
        EQCEGNRS GNEANV                                          L +KESL++E+    +                           
Subjt:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------

Query:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS
         DLALRCCLTKQSNHV+ E C DRPKL GLA  T CHHLCQWKHYT+
Subjt:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS

XP_022991622.1 tRNA:m(4)X modification enzyme TRM13 homolog [Cucurbita maxima]

4.3e-54

50.61

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Query:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG
        +SSDE   GL DSISSEM+RN++YGM GAEFHRLL KI+ALHGLICKDIQDSYRIKEACNMWIKGEID KIPFQE                    NYDGG
Subjt:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG

Query:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------
        EQCEGNRS GNEANV                                          L +KESL++E+    +                           
Subjt:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------

Query:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS
         DLALRCCLTKQSNHV+ E C DRPKL GLA  T CHHLCQWKHYT+
Subjt:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS

XP_023547814.1 tRNA:m(4)X modification enzyme TRM13 homolog [Cucurbita pepo subsp. pepo]

9.7e-54

50.61

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Query:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG
        +SSDE   G  DSISSEM+RNI+YGM GAEFHRLL KI+ALHGLICKDIQDSYRIKEACNMWIKGEID KIPFQE                    NYDGG
Subjt:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG

Query:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------
        EQCEGNRS GNEANV                                          L +KESL++E+    +                           
Subjt:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------

Query:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS
         DLALRCCLTKQSNHV+ E C DRPKL GLA  T CHHLCQWKHYT+
Subjt:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS

TrEMBL top hits

e value

%identity

Alignment

A0A1S3CFX2 tRNA:m(4)X modification enzyme TRM13

1.3e-48

47.37

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Query:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG
        MS++ P  GL DSISSEM+RNI+YGM G +FHRLL KI+ALH LICKDIQDSY+IKEACNMWIKGEID KIPFQE                    NYDGG
Subjt:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG

Query:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------
        +Q EGNRSYGN+ANVV                                         L +KESLI+E+    +                           
Subjt:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------

Query:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS
         DLALRCCLTKQ N  D E C D+ KL GLA  T CHHLCQWKHYT+
Subjt:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS

A0A1S4E343 tRNA:m(4)X modification enzyme TRM13

1.3e-48

47.37

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Query:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG
        MS++ P  GL DSISSEM+RNI+YGM G +FHRLL KI+ALH LICKDIQDSY+IKEACNMWIKGEID KIPFQE                    NYDGG
Subjt:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG

Query:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------
        +Q EGNRSYGN+ANVV                                         L +KESLI+E+    +                           
Subjt:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------

Query:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS
         DLALRCCLTKQ N  D E C D+ KL GLA  T CHHLCQWKHYT+
Subjt:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS

A0A5D3CJH7 tRNA:m(4)X modification enzyme TRM13

1.3e-48

47.37

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Query:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG
        MS++ P  GL DSISSEM+RNI+YGM G +FHRLL KI+ALH LICKDIQDSY+IKEACNMWIKGEID KIPFQE                    NYDGG
Subjt:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG

Query:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------
        +Q EGNRSYGN+ANVV                                         L +KESLI+E+    +                           
Subjt:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------

Query:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS
         DLALRCCLTKQ N  D E C D+ KL GLA  T CHHLCQWKHYT+
Subjt:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS

A0A6J1GSU8 tRNA:m(4)X modification enzyme TRM13

1.6e-54

51.01

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Query:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG
        +SSDE   GL DSISSEM+RNI+YGM GAEFHRLL KI+ALHGLICKDIQDSYRIKEACNMWIKGEID KIPFQE                    NYDGG
Subjt:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG

Query:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------
        EQCEGNRS GNEANV                                          L +KESL++E+    +                           
Subjt:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------

Query:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS
         DLALRCCLTKQSNHV+ E C DRPKL GLA  T CHHLCQWKHYT+
Subjt:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS

A0A6J1JMC6 tRNA:m(4)X modification enzyme TRM13

2.1e-54

50.61

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Query:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG
        +SSDE   GL DSISSEM+RN++YGM GAEFHRLL KI+ALHGLICKDIQDSYRIKEACNMWIKGEID KIPFQE                    NYDGG
Subjt:  MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQE--------------------NYDGG

Query:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------
        EQCEGNRS GNEANV                                          L +KESL++E+    +                           
Subjt:  EQCEGNRSYGNEANVV-----------------------------------------LAEKESLIIEQRESTVR--------------------------

Query:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS
         DLALRCCLTKQSNHV+ E C DRPKL GLA  T CHHLCQWKHYT+
Subjt:  -DLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTS

SwissProt top hits

e value

%identity

Alignment

Q10B19 tRNA:m(4)X modification enzyme TRM13

1.3e-05

28.12

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Query:  ISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQENY
        ++S  +R +++ +   E   L+ KIK  H      ++DS+ + +AC+ W++ ++D K+P+QE +
Subjt:  ISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQENY

Arabidopsis top hits

e value

%identity

Alignment

AT4G01880.1 methyltransferases

9.4e-23

29.75

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Query:  DEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQENY---DGG------------------EQ
        +E  +G    ++SEM+RN++Y M  ++FH+L++KI+ +HG ICKDI+DSY   EACN+W   EID K+PFQE +    G                   E 
Subjt:  DEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQENY---DGG------------------EQ

Query:  CEGN----------------------------------------RSYGNEANVVLAEKESLIIEQRESTVR---------------------------DL
        CE +                                        +SY  +A+  L +KESL++E+    +                            DL
Subjt:  CEGN----------------------------------------RSYGNEANVVLAEKESLIIEQRESTVR---------------------------DL

Query:  ALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHY
        +LRCCL++Q          + P L GLA  T CHHL QWK Y
Subjt:  ALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHY

Sequences

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CDS sequence

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ATGTCTTCCGATGAACCAACTCTGGGTTTATTAGATAGCATTTCCTCTGAAATGCAGAGGAATATCATTTATGGCATGTTTGGAGCTGAGTTTCATCGACTTCTTAGAAA
GATTAAAGCTCTTCATGGTCTTATATGCAAGGATATTCAAGACTCTTATAGGATAAAGGAAGCTTGCAATATGTGGATTAAAGGGGAAATAGATATGAAAATTCCATTTC
AAGAGAATTATGATGGAGGGGAACAATGTGAGGGTAACAGATCATATGGCAATGAGGCTAATGTTGTCCTAGCGGAGAAAGAGAGCTTGATTATAGAGCAAAGAGAATCG
ACAGTAAGAGATTTGGCTCTTAGATGTTGCCTTACCAAACAATCTAATCATGTCGATATGGAACCATGCGCAGATAGGCCGAAATTGATAGGGTTGGCTGCAGTGACTTC
TTGTCATCACCTTTGTCAATGGAAGCATTACACAAGTACAACAACTTATTGCAGTGCTAAATGCCTTAGCAGCAATGTAATTGCTGTGAATGAATTAGCTGAAAAACATA
GGATACCTTTGCTGGATACATGGTGGCATCTACTGGAATACTTTCAGAAATATATATCTTCAACTTCAATACTTTTTCTGCATAATCAAAGATACTTCAATTGGCATTTC
ATGAAGCTGTCATTTTGTAGTGGGCTCGAGCAAGAACCAGAGCTGTCAAGATTGATCCACAACATTAATATAGCCACAATCTACGATGCTATTATGTTAAATGGCTTCAC
TATCAGAGTGGACAACCCTTTTTCTCTTGGTTTTGAGGACTGTCATGATTACAATGATGCAGAGGAGGAGGCGTATAAGGAAGGACCATATCATGCAGGTGTTTAA

mRNA sequence

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Protein sequence

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MSSDEPTLGLLDSISSEMQRNIIYGMFGAEFHRLLRKIKALHGLICKDIQDSYRIKEACNMWIKGEIDMKIPFQENYDGGEQCEGNRSYGNEANVVLAEKESLIIEQRES
TVRDLALRCCLTKQSNHVDMEPCADRPKLIGLAAVTSCHHLCQWKHYTSTTTYCSAKCLSSNVIAVNELAEKHRIPLLDTWWHLLEYFQKYISSTSILFLHNQRYFNWHF
MKLSFCSGLEQEPELSRLIHNINIATIYDAIMLNGFTIRVDNPFSLGFEDCHDYNDAEEEAYKEGPYHAGV

Spg026416 (gene) of Sponge gourd (cylindrica) v1 genome