CuGenDBv2

Gene ID	Spg028081
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	DNA primase
Genome location	scaffold2:45761830..45763839
RNA-Seq Expression	Spg028081
Synteny	Spg028081
Gene Ontology terms	GO:0006269 - DNA replication, synthesis of RNA primer (biological process) GO:0006865 - amino acid transport (biological process) GO:0009734 - auxin-activated signaling pathway (biological process) GO:0055085 - transmembrane transport (biological process) GO:0005658 - alpha DNA polymerase:primase complex (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0003896 - DNA primase activity (molecular function) GO:0015293 - symporter activity (molecular function) GO:0046872 - metal ion binding (molecular function)
InterPro domains	IPR002755 - DNA primase, small subunit IPR014052 - DNA primase, small subunit, eukaryotic/archaeal

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0039338.1 DNA primase, small subunit [Cucumis melo var. makuwa]

4.5e-38

87.64

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Query:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGETGSKLCR
        AQGMRRHVEEIVFSF YPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+PE CDEFDPTTVPTVAQLLEELNA DM VD ETG K+ R
Subjt:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGETGSKLCR

KAG6592333.1 DNA primase small subunit, partial [Cucurbita argyrosperma subsp. sororia]

1.1e-36

90.36

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Query:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGET
        +QGMRRHVEEIVFSF YPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+PERCDEFDPTTVPTVAQLLEELNA DM VD ET
Subjt:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGET

KAG7025155.1 DNA primase small subunit [Cucurbita argyrosperma subsp. argyrosperma]

1.1e-36

90.36

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Query:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGET
        +QGMRRHVEEIVFSF YPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+PERCDEFDPTTVPTVAQLLEELNA DM VD ET
Subjt:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGET

XP_022973496.1 DNA primase small subunit [Cucurbita maxima]

1.1e-36

90.36

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Query:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGET
        +QGMRRHVEEIVFSF YPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+PERCDEFDPTTVPTVAQLLEELNA DM VD ET
Subjt:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGET

XP_023535939.1 DNA primase small subunit [Cucurbita pepo subsp. pepo]

1.1e-36

90.36

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Query:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGET
        +QGMRRHVEEIVFSF YPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+PERCDEFDPTTVPTVAQLLEELNA DM VD ET
Subjt:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGET

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KUP7 DNA primase

7.0e-37

91.46

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Query:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGE
        AQGMRRHVEEIVFSF YPRLDSEVTKHMNHLLKAPFCVHPKTGR+CVPI+PE CDEFDPTTVPTVAQLLEELNA DM VD E
Subjt:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGE

A0A5A7T8Y3 DNA primase

2.2e-38

87.64

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Query:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGETGSKLCR
        AQGMRRHVEEIVFSF YPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+PE CDEFDPTTVPTVAQLLEELNA DM VD ETG K+ R
Subjt:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGETGSKLCR

A0A6J1CKZ3 DNA primase

2.0e-36

90.24

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Query:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGE
        AQGMRRHVEEIVFSF YPRLD EVTKH NHLLKAPFCVHPKTGR+CVPINPE CDEFDPTTVPT+AQLLEELNA DM VDGE
Subjt:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGE

A0A6J1EEZ9 DNA primase

2.7e-36

91.36

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Query:  GMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGET
        GMRRHVEEIVFSF YPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+PERCDEFDPTTVPTVAQLLEELNA DM VD ET
Subjt:  GMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGET

A0A6J1I8T4 DNA primase

5.3e-37

90.36

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Query:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGET
        +QGMRRHVEEIVFSF YPRLD EVTKHMNHLLKAPFCVHPKTGR+CVPI+PERCDEFDPTTVPTVAQLLEELNA DM VD ET
Subjt:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGET

SwissProt top hits

e value

%identity

Alignment

O14215 DNA primase small subunit

2.2e-19

63.08

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Query:  EEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELN
        ++IV +++YPRLD EV++H+NHLLK+PFCVHP T RVCVPI+ ER D F+P  VPTV  LL+EL+
Subjt:  EEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELN

P49642 DNA primase small subunit

8.5e-16

55.38

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Query:  EIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNA
        EI+  + +PRLD  V+K +NHLLK+PF VHPKTGR+ VPI+ ++ D+FDP TVPT++ +  EL+A
Subjt:  EIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNA

Q24317 DNA primase small subunit

3.7e-19

57.53

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Query:  MRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGD
        ++  V EI    +YPRLD  VT+  NHLLKAPFC+HP TG+VCVP +     +FDPTTVPT+ QLL E+NA D
Subjt:  MRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGD

Q25998 DNA primase small subunit

8.5e-16

52.86

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Query:  MRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELN
        M   V+EIVF F YPRLD  V+K +NHLLK+PFC+H  TGRVCVP++ +  + F+P +VPT+  L E+ +
Subjt:  MRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELN

Q7KQM1 DNA primase small subunit

8.5e-16

52.86

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Query:  MRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELN
        M   V+EIVF F YPRLD  V+K +NHLLK+PFC+H  TGRVCVP++ +  + F+P +VPT+  L E+ +
Subjt:  MRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELN

Arabidopsis top hits

e value

%identity

Alignment

AT5G41880.1 DNA primases;DNA primases

1.8e-29

68.75

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Query:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVD
        A  +R  VEEIVF+F YPR+D EV+K MNHLLKAPFCVHPKTGRVCVPI+P  CDEFDP  VPT++QL+EE+N+G + +D
Subjt:  AQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVD

Sequences

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CDS sequence

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ATGATGTTGGCACAAGGAATGCGTAGGCATGTTGAAGAGATTGTTTTCTCTTTTATCTACCCTAGGCTTGATTCAGAGGTTACTAAACACATGAACCATCTGCTAAAAGC
ACCATTCTGTGTTCATCCCAAAACAGGTCGTGTTTGTGTCCCTATCAACCCTGAACGATGTGATGAGTTTGATCCAACTACTGTACCAACTGTTGCCCAGCTTCTGGAAG
AACTTAATGCAGGGGATATGATAGTAGATGGTGAGACTGGCTCGAAATTGTGCAGGAGTTACTATCGCAGTCTATTAGGGCTAGGCTACTAG

mRNA sequence

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Protein sequence

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MMLAQGMRRHVEEIVFSFIYPRLDSEVTKHMNHLLKAPFCVHPKTGRVCVPINPERCDEFDPTTVPTVAQLLEELNAGDMIVDGETGSKLCRSYYRSLLGLGY

Spg028081 (gene) of Sponge gourd (cylindrica) v1 genome