CuGenDBv2

Gene ID	Spg028141
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	tRNA-specific 2-thiouridylase MnmA
Genome location	scaffold2:44669204..44676081
RNA-Seq Expression	Spg028141
Synteny	Spg028141
Gene Ontology terms	GO:0005840 - ribosome (cellular component) GO:0003735 - structural constituent of ribosome (molecular function) GO:0016783 - sulfurtransferase activity (molecular function)
InterPro domains	IPR023382 - Adenine nucleotide alpha hydrolase-like domains

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6595682.1 39S ribosomal protein L46, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia]

1.5e-29

90.67

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Query:  FKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR
        F+MQ SF L GQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR+YPD+FLDKANAR
Subjt:  FKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR

KAG7027644.1 hypothetical protein SDJN02_11659 [Cucurbita argyrosperma subsp. argyrosperma]

1.5e-29

90.67

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Query:  FKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR
        F+MQ SF L GQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR+YPD+FLDKANAR
Subjt:  FKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR

XP_022925098.1 39S ribosomal protein L46, mitochondrial-like isoform X2 [Cucurbita moschata]

2.5e-29

91.89

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Query:  KMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR
        +MQ SF LAGQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR+YPD+FLDKANAR
Subjt:  KMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR

XP_022966446.1 39S ribosomal protein L46, mitochondrial-like isoform X1 [Cucurbita maxima]

2.5e-29

91.89

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Query:  KMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR
        +MQ SF LAGQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR+YPD+FLDKANAR
Subjt:  KMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR

XP_022992541.1 54S ribosomal protein L17, mitochondrial-like isoform X1 [Cucurbita maxima]

8.6e-30

88.31

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Query:  NNFKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR
        + F+MQRSF LA QL KRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR+YPD+FLDKANAR
Subjt:  NNFKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR

TrEMBL top hits

e value

%identity

Alignment

A0A6J1EB49 39S ribosomal protein L46, mitochondrial-like isoform X3

2.1e-29

93.15

Show/hide

Query:  MQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR
        MQ SF LAGQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR+YPD+FLDKANAR
Subjt:  MQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR

A0A6J1EEA2 39S ribosomal protein L46, mitochondrial-like isoform X2

1.2e-29

91.89

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Query:  KMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR
        +MQ SF LAGQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR+YPD+FLDKANAR
Subjt:  KMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR

A0A6J1HPC7 39S ribosomal protein L46, mitochondrial-like isoform X1

1.2e-29

91.89

Show/hide

Query:  KMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR
        +MQ SF LAGQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR+YPD+FLDKANAR
Subjt:  KMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR

A0A6J1HRN8 39S ribosomal protein L46, mitochondrial-like isoform X2

2.1e-29

93.15

Show/hide

Query:  MQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR
        MQ SF LAGQLVKRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR+YPD+FLDKANAR
Subjt:  MQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR

A0A6J1JTU9 54S ribosomal protein L17, mitochondrial-like isoform X1

4.2e-30

88.31

Show/hide

Query:  NNFKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR
        + F+MQRSF LA QL KRRGFSTNS+KIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRR+YPD+FLDKANAR
Subjt:  NNFKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR

SwissProt top hits

e value

%identity

Alignment

B2RHP0 tRNA-specific 2-thiouridylase MnmA

3.4e-13

48.44

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Query:  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNN
        L  I +++F+ R++G++E  I+E E G  +G+H+G+WF+ IGQ +GL L GGPW+VV KD K N
Subjt:  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNN

Q5LFN5 tRNA-specific 2-thiouridylase MnmA 2

4.4e-13

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Query:  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNN
        L  I +++FV R +GE+E  ++E E G  +G HRG+WF+ IGQ +GL L GGPW+VV KD ++N
Subjt:  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNN

Q64WG8 tRNA-specific 2-thiouridylase MnmA 2

4.4e-13

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Query:  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNN
        L  I +++FV R +GE+E  ++E E G  +G HRG+WF+ IGQ +GL L GGPW+VV KD ++N
Subjt:  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNN

Q7MAW9 tRNA-specific 2-thiouridylase MnmA

3.4e-13

48.44

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Query:  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNN
        L  I +++F+ R++G++E  I+E E G  +G+H+G+WF+ IGQ +GL L GGPW+VV KD K N
Subjt:  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNN

Q8ABF5 tRNA-specific 2-thiouridylase MnmA 1

5.8e-13

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Query:  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNN
        L  I +++FV R +GE E  I+E E G  +G HRG+WF+ IGQ +GL L GGPW+V+ KD + N
Subjt:  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNN

Arabidopsis top hits

e value

%identity

Alignment

AT1G14620.1 decoy

4.6e-21

77.78

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Query:  LVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR
        L  RRGF T+SDKIVASVLFERL VVIPK DP VYAFQEFKF WQQQ+RR+YPD+FLD A  R
Subjt:  LVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR

AT1G14620.2 decoy

4.6e-21

77.78

Show/hide

Query:  LVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR
        L  RRGF T+SDKIVASVLFERL VVIPK DP VYAFQEFKF WQQQ+RR+YPD+FLD A  R
Subjt:  LVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANAR

AT1G51310.1 transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases

2.6e-24

78.12

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Query:  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNN
        L  IKFS+FV RHIGE E +ILEAE G+FLG HRGFWFY IGQ QGLRLPGGPWYVV KD KNN
Subjt:  LDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNN

Sequences

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CDS sequence

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ATGTTCGGTTCAGTTTTCGGTTTGTCTTTGTCCGATCCGAACCAAGATGCGCTTGCTTATGCAGACCTTGAATCGATCTCTCAGCCGTCTCTCTGGTCTCTCAAACCGAT
CTTACAGCTGAATCAGTTTGCGCCATTGCTCGACGACATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAAGAGGAATGTGTCATATTGGAGGCGGAGTTGGGGA
ATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACGTGATTGGCCAATGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTTGTGGGAAAAGATAGAAAGAACAAT
TTCAAAATGCAAAGATCGTTCTGTTTAGCCGGTCAACTGGTGAAGAGGCGAGGATTTTCGACGAATTCCGATAAAATTGTGGCATCTGTACTGTTTGAAAGGCTGCCAGT
CGTGATTCCGAAGATTGATCCTGTTGTCTATGCATTTCAGGAGTTTAAATTTAGGTGGCAACAACAATATCGACGCAAATATCCTGATAAATTTTTAGATAAAGCTAATG
CCAGATTGTACAGCAGTGAGGAGATAAAGCCATGTGAGGGAGGAGTGCGATTGGGAGGGAGTGGGCTGCCCGCTGCTGTGTTGTGCGGCTGGAATCGAGAAGGAAGGGAG
ATACTGAGATTTAGAGCCCCAGAAGCGCCCAAGGCGCTCAAGGGCTTCGAGGAAGATGCGCGCCTTGGCCGCGCCTTCCCCACCGCGCCTCGCTTTTTCGAAGCGAGGCG
CTAA

mRNA sequence

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Protein sequence

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MFGSVFGLSLSDPNQDALAYADLESISQPSLWSLKPILQLNQFAPLLDDIKFSEFVARHIGEEECVILEAELGNFLGKHRGFWFYVIGQCQGLRLPGGPWYVVGKDRKNN
FKMQRSFCLAGQLVKRRGFSTNSDKIVASVLFERLPVVIPKIDPVVYAFQEFKFRWQQQYRRKYPDKFLDKANARLYSSEEIKPCEGGVRLGGSGLPAAVLCGWNREGRE
ILRFRAPEAPKALKGFEEDARLGRAFPTAPRFFEARR

Spg028141 (gene) of Sponge gourd (cylindrica) v1 genome