CuGenDBv2

Gene ID	Spg029256
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RNase H domain-containing protein
Genome location	scaffold12:33552499..33555543
RNA-Seq Expression	Spg029256
Synteny	Spg029256
Gene Ontology terms	GO:0044237 - cellular metabolic process (biological process) GO:0003676 - nucleic acid binding (molecular function) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

CAB4274760.1 unnamed protein product [Prunus armeniaca]

2.9e-09

37.17

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Query:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ
        P +  + + W  P L +LK+N DA+WS+    GG+GW++RDS G L+ AG +      S +M+E LA++  LS+  N        ++VESDS+  I +L 
Subjt:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ

Query:  GQDSVLSEVRNIV
        G+ +V S++  IV
Subjt:  GQDSVLSEVRNIV

CAB4274761.1 unnamed protein product [Prunus armeniaca]

2.9e-09

37.17

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Query:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ
        P +  + + W  P L +LK+N DA+WS+    GG+GW++RDS G L+ AG +      S +M+E LA++  LS+  N        ++VESDS+  I +L 
Subjt:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ

Query:  GQDSVLSEVRNIV
        G+ +V S++  IV
Subjt:  GQDSVLSEVRNIV

CAB4291403.1 unnamed protein product [Prunus armeniaca]

2.2e-09

38.05

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Query:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ
        P +  + + W  PP  +LK+N DA+WS+    GG+GW++RDS G L+ AG +      S LM+E LA++  LS+  N        ++VESDS+  I +L 
Subjt:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ

Query:  GQDSVLSEVRNIV
        G+ +V S++  IV
Subjt:  GQDSVLSEVRNIV

CAB4303756.1 unnamed protein product [Prunus armeniaca]

2.9e-09

37.17

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Query:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ
        P +  + + W  PP  +LK+N DA+WS+    GG+GW++RDS G L+ AG +      S +M+E LA++  LS+  N        ++VESDS+  I +L 
Subjt:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ

Query:  GQDSVLSEVRNIV
        G+ +V S++  IV
Subjt:  GQDSVLSEVRNIV

XP_022155262.1 uncharacterized protein LOC111022403 [Momordica charantia]

3.1e-11

30.87

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Query:  SRLMQRGVQFPPDLGLSNWDALTFWPKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKY
        S ++Q+  +F  +    +  +L+   K   + + W PPP+ +  LN DASWS S+  GG+GW++R   G ++LAG +  E   ++ +LEA A+ EGL   
Subjt:  SRLMQRGVQFPPDLGLSNWDALTFWPKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKY

Query:  LNHSACKSIPLMVESDSKTLINILQGQDS-------VLSEVRNIVDLAE
         N    +  PL +E+DS  + ++L  +         V+ E+ N+ D  E
Subjt:  LNHSACKSIPLMVESDSKTLINILQGQDS-------VLSEVRNIVDLAE

TrEMBL top hits

e value

%identity

Alignment

A0A6J1DNV9 uncharacterized protein LOC111022403

1.5e-11

30.87

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Query:  SRLMQRGVQFPPDLGLSNWDALTFWPKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKY
        S ++Q+  +F  +    +  +L+   K   + + W PPP+ +  LN DASWS S+  GG+GW++R   G ++LAG +  E   ++ +LEA A+ EGL   
Subjt:  SRLMQRGVQFPPDLGLSNWDALTFWPKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKY

Query:  LNHSACKSIPLMVESDSKTLINILQGQDS-------VLSEVRNIVDLAE
         N    +  PL +E+DS  + ++L  +         V+ E+ N+ D  E
Subjt:  LNHSACKSIPLMVESDSKTLINILQGQDS-------VLSEVRNIVDLAE

A0A6J5UE59 Reverse transcriptase domain-containing protein

1.4e-09

37.17

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Query:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ
        P +  + + W  P L +LK+N DA+WS+    GG+GW++RDS G L+ AG +      S +M+E LA++  LS+  N        ++VESDS+  I +L 
Subjt:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ

Query:  GQDSVLSEVRNIV
        G+ +V S++  IV
Subjt:  GQDSVLSEVRNIV

A0A6J5UF50 Uncharacterized protein

1.4e-09

37.17

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Query:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ
        P +  + + W  P L +LK+N DA+WS+    GG+GW++RDS G L+ AG +      S +M+E LA++  LS+  N        ++VESDS+  I +L 
Subjt:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ

Query:  GQDSVLSEVRNIV
        G+ +V S++  IV
Subjt:  GQDSVLSEVRNIV

A0A6J5VSP2 Uncharacterized protein

1.1e-09

38.05

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Query:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ
        P +  + + W  PP  +LK+N DA+WS+    GG+GW++RDS G L+ AG +      S LM+E LA++  LS+  N        ++VESDS+  I +L 
Subjt:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ

Query:  GQDSVLSEVRNIV
        G+ +V S++  IV
Subjt:  GQDSVLSEVRNIV

A0A6J5WPU6 Reverse transcriptase domain-containing protein

1.4e-09

37.17

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Query:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ
        P +  + + W  PP  +LK+N DA+WS+    GG+GW++RDS G L+ AG +      S +M+E LA++  LS+  N        ++VESDS+  I +L 
Subjt:  PKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQ

Query:  GQDSVLSEVRNIV
        G+ +V S++  IV
Subjt:  GQDSVLSEVRNIV

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT1G27870.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

3.6e-05

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Query:  KNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQG
        + +++H  W  P    +K N D S+ +       GW+VRDS+GS +LAG+    K  + L  E  AL   +    +H   +   +  E D+K L +++ G
Subjt:  KNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQG

AT2G34320.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein

5.0e-07

30.21

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Query:  VFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQGQD
        V W  PP   +K N DA+W   +   G+GW++R+ SG ++  G +   +  ++L  E  AL+  +   L  S      ++ ESD++ L+N+L   D
Subjt:  VFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPLMVESDSKTLINILQGQD

Sequences

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CDS sequence

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ATGGAAGCTGGAACGAGAGCCTTATTAAAGAAAGCCCAAGAAGTGGAAGAAATTCTTAGCATCCCTTTGGGAAGTAAAGAGATTATGGATGAGATTATATGGGCT
GAGCATCCTAAGGGTGTTAGGTTTTATGCCCTAAAACTCGTAGATAGTCCATTAGGTCCCACTGGACCCCTGGGGTGGGGTGCAAGACATCCCCTTCATTCTCCT
ATTCTCAGAGAATTCCCCTCAAGGCTTCCTCTAGACTCCCACCAGTCTCCTGCCTCTAGAAGTCTCAGAGTCATACCGGTGGGACCTTCACGGTTAATGCAAAGA
GGAGTCCAATTCCCACCTGATCTGGGGCTGTCAAATTGGGATGCTCTAACCTTTTGGCCGAAGAACCAGGAGAGTCATGTTTTCTGGTCTCCTCCGCCCCTATCA
GTGCTGAAGCTGAACATTGACGCTTCGTGGAGCAGCTCAAGCAGCAGCGGGGGTCTAGGGTGGTTGGTTCGTGACTCCTCAGGGTCTTTGATCTTGGCTGGTAGA
AAGAAAGCTGAGAAGAATTGGAGTATTTTAATGCTAGAAGCTTTAGCGTTGAAAGAAGGGCTTTCAAAATACCTTAATCACAGTGCATGCAAGTCCATTCCTCTC
ATGGTGGAATCAGACTCGAAGACGCTGATCAATATTCTTCAAGGGCAAGATTCAGTTCTCTCGGAGGTGAGGAACATCGTTGATCTAGCGGAGCGCGAGCAGCAG
CGGAGATCTCCTTTAGTTGGTGCCCGCAAGGTTGCAATCGAGCAGTCCATGTGTTAG

mRNA sequence

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Protein sequence

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MEAGTRALLKKAQEVEEILSIPLGSKEIMDEIIWAEHPKGVRFYALKLVDSPLGPTGPLGWGARHPLHSPILREFPSRLPLDSHQSPASRSLRVIPVGPSRLMQR
GVQFPPDLGLSNWDALTFWPKNQESHVFWSPPPLSVLKLNIDASWSSSSSSGGLGWLVRDSSGSLILAGRKKAEKNWSILMLEALALKEGLSKYLNHSACKSIPL
MVESDSKTLINILQGQDSVLSEVRNIVDLAEREQQRRSPLVGARKVAIEQSMC

Spg029256 (gene) of Sponge gourd (cylindrica) v1 genome