CuGenDBv2

Gene ID	Spg029706
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Prolyl-tRNA synthetase
Genome location	scaffold6:9256920..9262775
RNA-Seq Expression	Spg029706
Synteny	Spg029706
Gene Ontology terms	GO:0006433 - prolyl-tRNA aminoacylation (biological process) GO:0005737 - cytoplasm (cellular component) GO:0004827 - proline-tRNA ligase activity (molecular function) GO:0005524 - ATP binding (molecular function)
InterPro domains	IPR004499 - Proline-tRNA ligase, class IIa, archaeal-type

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6574195.1 Proline--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia]

6.6e-19

32.99

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Query:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT
        R KPFVR LEF W EG T  A PEEAEKE                                                                     FT
Subjt:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT

Query:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------
        DENG+RQHV+ +  W ++   +   +++ GDDGGLMLP  LAP+        +K+  KAEV +AA+SV+ NL++AGIKVKLDDS  R   +         
Subjt:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------

Query:  -----PAPPIDI-----------------YTFILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS
                P D+                   F +S+ P++                         SN+VDVSSYNELKEAISQGK AR PWSAS
Subjt:  -----PAPPIDI-----------------YTFILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS

XP_004149956.3 proline--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus]

2.7e-20

34.01

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Query:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT
        R KPFVR LEF W EG T  A PEEAEKE                                                                     FT
Subjt:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT

Query:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------
        DENG+RQHV+ +  W ++   +   +++ GDDGGLMLP  LAP+        +KD DKAEV +AASSVKE LQ+AG+KVKLD+S  +   +         
Subjt:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------

Query:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS
                P D+ +                 F +S+ P+V                         SNIVDVSSYNELKEAIS+GK AR PWSAS
Subjt:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS

XP_008456101.1 PREDICTED: proline--tRNA ligase, chloroplastic/mitochondrial, partial [Cucumis melo]

2.7e-20

34.01

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Query:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT
        R KPFVR LEF W EG T  A PEEAEKE                                                                     FT
Subjt:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT

Query:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------
        DENG+RQHV+ +  W ++   +   +++ GDDGGLMLP  LAP+        +KD DKAEV +AASSVKE LQ+AG+KVKLD+S  +   +         
Subjt:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------

Query:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS
                P D+ +                 F +S+ P+V                         SNIVDVSSYNELKEAIS+GK AR PWSAS
Subjt:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS

XP_022136703.1 proline--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia]

5.4e-21

34.35

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Query:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT
        R KPFVR LEF W EG T  A PEEAEKE                                                                     F 
Subjt:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT

Query:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------
        DENG+RQHV+ +  W ++   +   +++ GDDGGLMLP  LAP+        +KD+D+AEV SA+SSVKE+LQ+AGIKVKLDDS  R   +         
Subjt:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------

Query:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS
                P D+ +                 F +S+ P++                         SNIVDVSSYNELKEAISQGK AR PWSAS
Subjt:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS

XP_038878181.1 proline--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida]

1.2e-20

34.01

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Query:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT
        R KPF+R LEF W EG T  A PEEAEKE                                                                     FT
Subjt:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT

Query:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------
        DENG+RQHV+ +  W ++   +   +++ GDDGGLMLP  LAP+        +KD DK EV SAASSVKE+LQ+AGIKVKLD+S  R   +         
Subjt:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------

Query:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS
                P D+ +                 F +S+ P+V                         SNIVDVSSY+ELKEAIS+GK AR PWSAS
Subjt:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS

TrEMBL top hits

e value

%identity

Alignment

A0A067JQI8 Prolyl-tRNA synthetase

9.3e-19

33.33

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Query:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT
        R KPFVR LEF W EG T  A PEEAEKE                                                                     FT
Subjt:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT

Query:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------
        DE+G+RQHV+ +  W ++   +   +++ GDD GLMLP  +AP+        +KDN+KA V SAASSVKE LQSAG+KVKLDDS  R   +         
Subjt:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------

Query:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS
                P D+ +                 F +S+ P++                         SNIVDVSSY+ELKEAIS GK AR PWSAS
Subjt:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS

A0A0A0L6I6 Prolyl-tRNA synthetase

1.3e-20

34.01

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Query:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT
        R KPFVR LEF W EG T  A PEEAEKE                                                                     FT
Subjt:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT

Query:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------
        DENG+RQHV+ +  W ++   +   +++ GDDGGLMLP  LAP+        +KD DKAEV +AASSVKE LQ+AG+KVKLD+S  +   +         
Subjt:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------

Query:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS
                P D+ +                 F +S+ P+V                         SNIVDVSSYNELKEAIS+GK AR PWSAS
Subjt:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS

A0A1S3C2J3 Prolyl-tRNA synthetase

1.3e-20

34.01

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Query:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT
        R KPFVR LEF W EG T  A PEEAEKE                                                                     FT
Subjt:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT

Query:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------
        DENG+RQHV+ +  W ++   +   +++ GDDGGLMLP  LAP+        +KD DKAEV +AASSVKE LQ+AG+KVKLD+S  +   +         
Subjt:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------

Query:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS
                P D+ +                 F +S+ P+V                         SNIVDVSSYNELKEAIS+GK AR PWSAS
Subjt:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS

A0A6J1C8A3 Prolyl-tRNA synthetase

2.6e-21

34.35

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Query:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT
        R KPFVR LEF W EG T  A PEEAEKE                                                                     F 
Subjt:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT

Query:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------
        DENG+RQHV+ +  W ++   +   +++ GDDGGLMLP  LAP+        +KD+D+AEV SA+SSVKE+LQ+AGIKVKLDDS  R   +         
Subjt:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------

Query:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS
                P D+ +                 F +S+ P++                         SNIVDVSSYNELKEAISQGK AR PWSAS
Subjt:  -----PAPPIDIYT-----------------FILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS

A0A6J1G067 Prolyl-tRNA synthetase

3.2e-19

32.99

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Query:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT
        R KPFVR LEF W EG T  A PEEAEKE                                                                     FT
Subjt:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT

Query:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------
        DENG+RQHV+ +  W ++   +   +++ GDDGGLMLP  LAP+        +K+  KAEV +AA+SV+ NL++AGIKVKLDDS  R   +         
Subjt:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF---------

Query:  -----PAPPIDI-----------------YTFILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS
                P D+                   F +S+ P++                         SN+VDVSSYNELKEAISQGK AR PWSAS
Subjt:  -----PAPPIDI-----------------YTFILSI-PTVC------------------------SNIVDVSSYNELKEAISQGKLARCPWSAS

SwissProt top hits

e value

%identity

Alignment

A5FA31 Proline--tRNA ligase

6.2e-04

30.85

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Query:  EFTDENGKRQHVYGRHHWQLAPHLLEEL-LSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF
        +F +  GK++HV+G   W ++  L+  L ++  DD GL+LP  LAP+        + D   A++ +A + +   L+   I VK DD   +K  F
Subjt:  EFTDENGKRQHVYGRHHWQLAPHLLEEL-LSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAF

Q9FYR6 Proline--tRNA ligase, chloroplastic/mitochondrial

2.6e-18

29.59

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Query:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT
        R KPF+R LEF W EG T  A PEEAEKE                                                                     F 
Subjt:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT

Query:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYR-------------
        DENG+RQHV+ +  W ++   +   +++ GDD GLMLP  +AP+        +KD +K  V SAASSVKE LQ+AG++VKLDD+  R             
Subjt:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYR-------------

Query:  --------------------KHAFPAPPIDIYTFILSIPTVC-----------------------SNIVDVSSYNELKEAISQGKLARCPWSAS
                            +   P     ++   +   T+                        SNIVDV+SY ELK+AIS GK AR PWSAS
Subjt:  --------------------KHAFPAPPIDIYTFILSIPTVC-----------------------SNIVDVSSYNELKEAISQGKLARCPWSAS

Arabidopsis top hits

e value

%identity

Alignment

AT5G52520.1 Class II aaRS and biotin synthetases superfamily protein

1.8e-19

29.59

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Query:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT
        R KPF+R LEF W EG T  A PEEAEKE                                                                     F 
Subjt:  RWKPFVRALEFFWLEGRT--ACPEEAEKE---------------------------------------------------------------------FT

Query:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYR-------------
        DENG+RQHV+ +  W ++   +   +++ GDD GLMLP  +AP+        +KD +K  V SAASSVKE LQ+AG++VKLDD+  R             
Subjt:  DENGKRQHVYGRHHWQLAPHLLEE-LLSFGDDGGLMLPSMLAPMS------GRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYR-------------

Query:  --------------------KHAFPAPPIDIYTFILSIPTVC-----------------------SNIVDVSSYNELKEAISQGKLARCPWSAS
                            +   P     ++   +   T+                        SNIVDV+SY ELK+AIS GK AR PWSAS
Subjt:  --------------------KHAFPAPPIDIYTFILSIPTVC-----------------------SNIVDVSSYNELKEAISQGKLARCPWSAS

Sequences

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CDS sequence

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ATGCCCGTTCCGTTCGCCGCCGATGGCTGCAAGCTTCTGTGCGGAAAGAACATTGAAATGTTAAGCTCAAGGAGACCGGTCGCAGAAACATGTACTTTCTACAGGTGTGG
CTCTGGCGTCGTTAAGAGTTTCATTGGGTTAATGTTAAAAGATGGGAGATGCAGATGGAAACCATTTGTCAGAGCTCTTGAATTTTTTTGGCTGGAGGGTCGTACTGCCT
GTCCAGAAGAGGCAGAAAAAGAGTTCACAGATGAAAATGGAAAAAGGCAACATGTATATGGCAGACATCATTGGCAATTAGCACCCCATTTGTTGGAGGAATTATTGTCT
TTTGGAGATGATGGAGGTTTAATGCTGCCTTCGATGCTTGCTCCAATGAGTGGAAGGAAGGATAATGATAAAGCAGAAGTTTTCAGCGCTGCATCTTCTGTAAAAGAAAA
TCTTCAAAGTGCAGGGATAAAGGTTAAACTTGATGACTCAGCATATCGTAAACATGCATTTCCTGCCCCTCCTATAGATATATATACCTTCATTTTGTCTATACCTACCG
TTTGCAGTAACATTGTGGATGTGAGTTCATATAATGAACTTAAAGAGGCAATCTCGCAGGGAAAATTGGCTAGGTGTCCTTGGTCAGCAAGTATGGACATTACTCTATCA
AATATTTTCTCTGGAATTACAATTCGCCCTCCCCATTTTATAGGAGATGTCCCAATGGAGAAGGCTTTAAGAGCCATGACTCTTTAA

mRNA sequence

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Protein sequence

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MPVPFAADGCKLLCGKNIEMLSSRRPVAETCTFYRCGSGVVKSFIGLMLKDGRCRWKPFVRALEFFWLEGRTACPEEAEKEFTDENGKRQHVYGRHHWQLAPHLLEELLS
FGDDGGLMLPSMLAPMSGRKDNDKAEVFSAASSVKENLQSAGIKVKLDDSAYRKHAFPAPPIDIYTFILSIPTVCSNIVDVSSYNELKEAISQGKLARCPWSASMDITLS
NIFSGITIRPPHFIGDVPMEKALRAMTL

Spg029706 (gene) of Sponge gourd (cylindrica) v1 genome