CuGenDBv2

Gene ID	Spg031329
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	endo-1,31,4-beta-D-glucanase
Genome location	scaffold11:38848747..38851882
RNA-Seq Expression	Spg031329
Synteny	Spg031329
Gene Ontology terms	GO:0016787 - hydrolase activity (molecular function)
InterPro domains	IPR002925 - Dienelactone hydrolase IPR029058 - Alpha/Beta hydrolase fold

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6597473.1 hypothetical protein SDJN03_10653, partial [Cucurbita argyrosperma subsp. sororia]

8.6e-34

57.45

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Query:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL
        G EKSFDDGKA+VEAL SKGIS+IG  G CWG                              +VK  IS+L AE D  SPP LLKQ EEAL+A P++D  
Subjt:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL

Query:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL
        VKIFPKVSHGWT+RY+VEDEEAVK ADEAH D+L WF KYL
Subjt:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL

KAG7028931.1 hypothetical protein SDJN02_10114 [Cucurbita argyrosperma subsp. argyrosperma]

8.6e-34

57.45

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Query:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL
        G EKSFDDGKA+VEAL SKGIS+IG  G CWG                              +VK  IS+L AE D  SPP LLKQ EEAL+A P++D  
Subjt:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL

Query:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL
        VKIFPKVSHGWT+RY+VEDEEAVK ADEAH D+L WF KYL
Subjt:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL

XP_023539324.1 endo-1,3;1,4-beta-D-glucanase isoform X1 [Cucurbita pepo subsp. pepo]

8.6e-34

56.74

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Query:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL
        G EKSFDDGKA+VEAL SKGIS+IG  G CWG                              +VK  +S+L AE D  SPP LLKQ EEAL+A P++D  
Subjt:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL

Query:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL
        VKIFPKVSHGWT+RY+VEDEEAVK ADEAH D+L WF KYL
Subjt:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL

XP_023539325.1 endo-1,3;1,4-beta-D-glucanase isoform X2 [Cucurbita pepo subsp. pepo]

8.6e-34

56.74

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Query:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL
        G EKSFDDGKA+VEAL SKGIS+IG  G CWG                              +VK  +S+L AE D  SPP LLKQ EEAL+A P++D  
Subjt:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL

Query:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL
        VKIFPKVSHGWT+RY+VEDEEAVK ADEAH D+L WF KYL
Subjt:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL

XP_024463622.1 endo-1,3;1,4-beta-D-glucanase isoform X2 [Populus trichocarpa]

1.7e-34

63.06

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Query:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWGDVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWLVKIFPKVSHGWTLRYDVEDEEAVKRADEAH
        G  K F+D K ++EAL SKG+S+IG  GFCWG+ K PISIL AE D  SPPALLKQ EE L++  ++D  VKIFPKV+HGWT+RYDVEDE AVK A+EAH
Subjt:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWGDVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWLVKIFPKVSHGWTLRYDVEDEEAVKRADEAH

Query:  HDMLDWFLKYL
         ++L+WF KY+
Subjt:  HDMLDWFLKYL

TrEMBL top hits

e value

%identity

Alignment

A0A2C9WAD3 DLH domain-containing protein

3.9e-32

51.41

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Query:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL
        G +K FDD KA+V+AL SKG+S+IG  GFCWG                              +VK PISIL AE D  SPPAL+KQ EE L+A  ++D+ 
Subjt:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL

Query:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYLN
        VKIFPKV+HGWT+RY+VEDE AV+ ADEAH ++LDWF K++N
Subjt:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYLN

A0A5D3CVZ9 Endo-1,3

1.5e-31

50.64

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Query:  PSSTNLPL---VAREGQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLK
        P   N P+   +   G +K FDD K +VEAL +KGIS+IG  G CWG                               VK  ISIL AE D  SPP LLK
Subjt:  PSSTNLPL---VAREGQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLK

Query:  QLEEALSANPKIDWLVKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL
        Q EE LSA P++D  VKIFPKVSHGWT+RY+VEDEEAVK ADEAH D+L WF KY+
Subjt:  QLEEALSANPKIDWLVKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL

A0A6J1C860 endo-1,31,4-beta-D-glucanase-like

1.5e-31

49.36

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Query:  PSSTNLPL---VAREGQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLK
        P   N P+   +   G +K F+D K +VEAL SKGIS+IG  GFCWG                              +VK  IS+L AE D  SPP LLK
Subjt:  PSSTNLPL---VAREGQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLK

Query:  QLEEALSANPKIDWLVKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL
        Q EE LSA P++D  VKIFPKV HGW++RY+VEDEEAVK A+EAH D+L WF KYL
Subjt:  QLEEALSANPKIDWLVKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL

A0A6J1F9P2 endo-1,31,4-beta-D-glucanase

5.4e-34

56.74

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Query:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL
        G EKSFDDGKA+VEAL SKGIS+IG  G CWG                              +VK  +S+L AE D  SPP LLKQ EEAL+A P++D  
Subjt:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL

Query:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL
        VKIFPKVSHGWT+RY+VEDEEAVK ADEAH D+L WF KYL
Subjt:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL

A0A6J1IDX2 endo-1,31,4-beta-D-glucanase

5.4e-34

56.03

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Query:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL
        G EKSFDDGKA+VEAL SKGIS+IG  G CWG                              +VK  +++L AE D  SPP LLKQ EEAL+A P++D  
Subjt:  GQEKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWL

Query:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL
        VKIFPKVSHGWT+RY+VEDEEAVK ADEAH D+L WF KYL
Subjt:  VKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL

SwissProt top hits

e value

%identity

Alignment

Q5XH09 Carboxymethylenebutenolidase homolog

1.7e-08

30.4

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Query:  ISSIGVGGFCWGDVKT-------------------------------PISILVAEFDDFSPPALLKQLEEALSANPKIDWLVKIFPKVSHGWTLR--YDV
        +  IGV GFCWG V T                               P   + AE D   P   +  LE+ L  + K+D+ +K+FPK +HG+  R   D+
Subjt:  ISSIGVGGFCWGDVKT-------------------------------PISILVAEFDDFSPPALLKQLEEALSANPKIDWLVKIFPKVSHGWTLR--YDV

Query:  EDEEAVKRADEAHHDMLDWFLKYLN
          E+     +EA  DML+W  KY+N
Subjt:  EDEEAVKRADEAHHDMLDWFLKYLN

Q6P7K0 Carboxymethylenebutenolidase homolog

2.2e-08

30.65

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Query:  ISSIGVGGFCWGDVKT-------------------------------PISILVAEFDDFSPPALLKQLEEALSANPKIDWLVKIFPKVSHGWTLRYDVE-
        +  IGV GFCWG V T                               P   + AE D   P   +  LEE L  + K+D+ VK+FPK +HG+  R + + 
Subjt:  ISSIGVGGFCWGDVKT-------------------------------PISILVAEFDDFSPPALLKQLEEALSANPKIDWLVKIFPKVSHGWTLRYDVE-

Query:  DEEAVKRADEAHHDMLDWFLKYLN
        + E     +EA  +ML+W  KY+N
Subjt:  DEEAVKRADEAHHDMLDWFLKYLN

Arabidopsis top hits

e value

%identity

Alignment

AT3G23570.1 alpha/beta-Hydrolases superfamily protein

3.1e-26

41.73

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Query:  EKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWLVK
        EK F++ K IVEAL +KGI+SIG  GFCWG                              +V  PI++L AE D  SPP L++Q E+ L++ P++   VK
Subjt:  EKSFDDGKAIVEALTSKGISSIGVGGFCWG------------------------------DVKTPISILVAEFDDFSPPALLKQLEEALSANPKIDWLVK

Query:  IFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL
        IFP+  HGWT+RY+  D   V+ A EAH DML W + YL
Subjt:  IFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL

AT3G23600.1 alpha/beta-Hydrolases superfamily protein

2.7e-30

42.04

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Query:  SPSSTNLPL---VAREGQEKSFDDGKAIVEALTSKGISSIGVGGFCWG---------------------------DV---KTPISILVAEFDDFSPPALL
        +PS+ + P+   +   G +K F++ K ++E + +KGI++IG  G CWG                           D+   K PI+IL AE D  SPPALL
Subjt:  SPSSTNLPL---VAREGQEKSFDDGKAIVEALTSKGISSIGVGGFCWG---------------------------DV---KTPISILVAEFDDFSPPALL

Query:  KQLEEALSANPKIDWLVKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL
        KQ EE LS+ P+++  VKI PKVSHGWT+RY++++ EAVK A+EAH +MLDWF+ Y+
Subjt:  KQLEEALSANPKIDWLVKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL

AT3G23600.2 alpha/beta-Hydrolases superfamily protein

5.5e-31

42.86

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Query:  SPSSTNLPL---VAREGQEKSFDDGKAIVEALTSKGISSIGVGGFCWGDV---------------------------KTPISILVAEFDDFSPPALLKQL
        +PS+ + P+   +   G +K F++ K ++E + +KGI++IG  G CWG V                           K PI+IL AE D  SPPALLKQ 
Subjt:  SPSSTNLPL---VAREGQEKSFDDGKAIVEALTSKGISSIGVGGFCWGDV---------------------------KTPISILVAEFDDFSPPALLKQL

Query:  EEALSANPKIDWLVKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL
        EE LS+ P+++  VKI PKVSHGWT+RY++++ EAVK A+EAH +MLDWF+ Y+
Subjt:  EEALSANPKIDWLVKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYL

Sequences

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CDS sequence

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ATGCAAGGAGCTAACGAGGACAACCGGGCGGAGATAGGACCAAGAAGCGACCCAGAGGAAGACCAGCCAACGGGCCGGGCCAAAATGGCCCGACCCAAGCACCACACCGG
TGTGCAGGTTACTCTTTTGCAGGCCACGTCTTCCCCCTCATCTACAAATTTACCGTTGGTGGCACGTGAAGGTCAGGAAAAGTCTTTTGATGATGGGAAGGCAATAGTTG
AAGCTTTGACAAGCAAAGGTATTTCGTCGATTGGAGTTGGAGGATTTTGTTGGGGTGATGTGAAGACTCCCATTTCAATACTAGTAGCCGAGTTCGATGATTTTTCTCCT
CCAGCACTCTTGAAACAGCTTGAAGAGGCCTTATCTGCAAATCCTAAGATTGATTGGTTGGTAAAGATATTTCCAAAAGTTTCTCATGGTTGGACTTTGAGGTACGATGT
TGAAGATGAAGAGGCTGTCAAACGAGCAGATGAGGCTCACCACGACATGTTGGATTGGTTTCTTAAGTACCTTAACTAA

mRNA sequence

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Protein sequence

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MQGANEDNRAEIGPRSDPEEDQPTGRAKMARPKHHTGVQVTLLQATSSPSSTNLPLVAREGQEKSFDDGKAIVEALTSKGISSIGVGGFCWGDVKTPISILVAEFDDFSP
PALLKQLEEALSANPKIDWLVKIFPKVSHGWTLRYDVEDEEAVKRADEAHHDMLDWFLKYLN

Spg031329 (gene) of Sponge gourd (cylindrica) v1 genome