CuGenDBv2

Gene ID	Spg032258
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Non-specific serine/threonine protein kinase
Genome location	scaffold2:33088223..33093465
RNA-Seq Expression	Spg032258
Synteny	Spg032258
Gene Ontology terms	GO:0006468 - protein phosphorylation (biological process) GO:0008033 - tRNA processing (biological process) GO:0070525 - tRNA threonylcarbamoyladenosine metabolic process (biological process) GO:0000408 - EKC/KEOPS complex (cellular component) GO:0005634 - nucleus (cellular component) GO:0004674 - protein serine/threonine kinase activity (molecular function) GO:0004712 - protein serine/threonine/tyrosine kinase activity (molecular function) GO:0005524 - ATP binding (molecular function) GO:0106310 - protein serine kinase activity (molecular function)
InterPro domains	IPR000719 - Protein kinase domain IPR008266 - Tyrosine-protein kinase, active site IPR011009 - Protein kinase-like domain superfamily IPR022495 - Serine/threonine-protein kinase Bud32

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6588447.1 EKC/KEOPS complex subunit bud32, partial [Cucurbita argyrosperma subsp. sororia]

2.1e-65

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Query:  VMEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKD
        VME KTDSNDGSL+LIKQGAE                                      AEARCMTKARRL VSTPVLYAVDPI YTLTFEYVEGRSVKD
Subjt:  VMEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKD

Query:  ILLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL
        ILLEIGSSG GSKQL DIA QIGVAIGKLHDGGLIHGDLTTSNML+RS TNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNV+
Subjt:  ILLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL

XP_022154600.1 EKC/KEOPS complex subunit bud32 [Momordica charantia]

4.7e-65

74.87

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Query:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI
        MEIKTDSNDGSLILIKQGAE                                      AEARCMTKARRLGVSTPVLYAVDP  +TLTFEYVEGR+VKDI
Subjt:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI

Query:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL
        LLEIGSSG G+KQLNDIA QIGVAIGKLHDGGL HGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGN++
Subjt:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL

XP_022971412.1 EKC/KEOPS complex subunit bud32 isoform X2 [Cucurbita maxima]

2.7e-65

74.23

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Query:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI
        MEIKTDSNDGSL+LIKQGAE                                      AEARCMTKARRL VSTPVLYAVDPI +TLTFEYVEGRSVKDI
Subjt:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI

Query:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVLARF
        LLEIGSSG GSKQL DIA QIGVAIGKLHDGGLIHGDLTTSNML+RS TNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNV++ F
Subjt:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVLARF

XP_023529406.1 EKC/KEOPS complex subunit bud32 [Cucurbita pepo subsp. pepo]

1.6e-65

75.39

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Query:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI
        MEIKTDSNDGSL+LIKQGAE                                      AEARCMTKARRL VSTPVLYAVDPI YTLTFEYVEGRSVKDI
Subjt:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI

Query:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL
        LLEIGSSG GSKQL DIA QIGVAIGKLHDGGLIHGDLTTSNML+RS TNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNV+
Subjt:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL

XP_031736114.1 EKC/KEOPS complex subunit bud32 isoform X2 [Cucumis sativus]

2.0e-68

90.73

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Query:  IKTDSNDGSLILIKQGAEAEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDILLEIGSSGG-SKQLNDIASQIGVAIGKLHDGGLIHGDLTT
        ++TDSNDG+LIL+KQGAEAEARCMTKARRLGV+TPVLYAVDPI YTLTFEYVEG  VKDILLEIGSSGG SKQL+DIA QIGVAIGKLHDGGL+HGDLTT
Subjt:  IKTDSNDGSLILIKQGAEAEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDILLEIGSSGG-SKQLNDIASQIGVAIGKLHDGGLIHGDLTT

Query:  SNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL
        SNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGN++
Subjt:  SNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL

TrEMBL top hits

e value

%identity

Alignment

A0A1S3BPU1 Non-specific serine/threonine protein kinase

1.5e-64

73.3

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Query:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI
        MEIKTDSNDG+LIL+KQGAE                                      AEARCMTKARRLGV+TPVLYAVDPI YTLTFEYVEG SVKDI
Subjt:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI

Query:  LLEIGSSGG-SKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL
        LLEIGS+GG SK+L+DIA QIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLS+HSSCGN++
Subjt:  LLEIGSSGG-SKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL

A0A5D3CF78 Non-specific serine/threonine protein kinase

1.5e-64

73.3

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Query:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI
        MEIKTDSNDG+LIL+KQGAE                                      AEARCMTKARRLGV+TPVLYAVDPI YTLTFEYVEG SVKDI
Subjt:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI

Query:  LLEIGSSGG-SKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL
        LLEIGS+GG SK+L+DIA QIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLS+HSSCGN++
Subjt:  LLEIGSSGG-SKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL

A0A6J1DK25 Non-specific serine/threonine protein kinase

2.3e-65

74.87

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Query:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI
        MEIKTDSNDGSLILIKQGAE                                      AEARCMTKARRLGVSTPVLYAVDP  +TLTFEYVEGR+VKDI
Subjt:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI

Query:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL
        LLEIGSSG G+KQLNDIA QIGVAIGKLHDGGL HGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGN++
Subjt:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL

A0A6J1I1V9 Non-specific serine/threonine protein kinase

1.3e-65

74.23

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Query:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI
        MEIKTDSNDGSL+LIKQGAE                                      AEARCMTKARRL VSTPVLYAVDPI +TLTFEYVEGRSVKDI
Subjt:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI

Query:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVLARF
        LLEIGSSG GSKQL DIA QIGVAIGKLHDGGLIHGDLTTSNML+RS TNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNV++ F
Subjt:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVLARF

A0A6J1I5M0 Non-specific serine/threonine protein kinase

3.0e-65

74.87

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Query:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI
        MEIKTDSNDGSL+LIKQGAE                                      AEARCMTKARRL VSTPVLYAVDPI +TLTFEYVEGRSVKDI
Subjt:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI

Query:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL
        LLEIGSSG GSKQL DIA QIGVAIGKLHDGGLIHGDLTTSNML+RS TNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNV+
Subjt:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVL

SwissProt top hits

e value

%identity

Alignment

Q4HYC1 EKC/KEOPS complex subunit BUD32

1.6e-20

44.52

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Query:  LIKQGAEAEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSV-KDILLEIGSS----GGSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRS
        L K    +EAR + K RR GV  P +YAVD     L  E+V G  V K I   +G+         +L D+  +IG AIG +H  G++HGDLTTSNM++  
Subjt:  LIKQGAEAEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSV-KDILLEIGSS----GGSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRS

Query:  GTN---------ELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMH
          N         ELV+ID GLS  S   ED+AVDLYVLERA  S H
Subjt:  GTN---------ELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMH

Q54W07 EKC/KEOPS complex subunit bud32

2.9e-25

48.8

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Query:  EARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDILLE-IGSSGGSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGL
        E R + K ++ G+  P LY VD     +  E+++G +VK  L +   S+    Q+  I  ++G  IG +H+  +IHGDLTTSNML+R  TNELV IDFGL
Subjt:  EARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDILLE-IGSSGGSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGL

Query:  SFTSTIPEDKAVDLYVLERALLSMH
        S+TS   EDKAVDLYVLERA +S H
Subjt:  SFTSTIPEDKAVDLYVLERALLSMH

Q96S44 EKC/KEOPS complex subunit TP53RK

3.2e-24

46.38

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Query:  EARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGR-SVKDILLEIGSSGGSKQ-LNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNEL--VLID
        EAR + + RR G+S PV++ VD     L  E +EG  +V+D +     +  + Q L+++A  IG  + ++HD  LIHGDLTTSNML++    +L  VLID
Subjt:  EARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGR-SVKDILLEIGSSGGSKQ-LNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNEL--VLID

Query:  FGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVLARF
        FGLSF S +PEDK VDLYVLE+A LS H +   V   F
Subjt:  FGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVLARF

Q99PW4 EKC/KEOPS complex subunit Tp53rk

1.1e-21

47.66

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Query:  EARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGR-SVKDILLEIGSSGGSKQ-LNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNEL--VLID
        EAR + + RR G++ PV++ VD     L  E +E   +V+D +     +    Q L D+A ++G  +  +HD  LIHGDLTTSNML+R    +L  VLID
Subjt:  EARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGR-SVKDILLEIGSSGGSKQ-LNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNEL--VLID

Query:  FGLSFTSTIPEDKAVDLYVLERALLSMH
        FGLSF S +PEDK VDLYVLE+A LS H
Subjt:  FGLSFTSTIPEDKAVDLYVLERALLSMH

Q9P7N1 EKC/KEOPS complex subunit SPAP27G11.07c

1.6e-20

42.28

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Query:  EARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDILLEIGSSGGSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNEL-VLIDFGL
        EAR + K   +G+  P+LY +D  +  +  E+++G  V+D + EI      K+L  +  +IG  + K+H   ++HGDLTTSNM++ S  N + + IDFGL
Subjt:  EARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDILLEIGSSGGSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNEL-VLIDFGL

Query:  SFTSTIPEDKAVDLYVLERALLS
           S   EDKAVD+YVLERAL S
Subjt:  SFTSTIPEDKAVDLYVLERALLS

Arabidopsis top hits

e value

%identity

Alignment

AT5G26110.1 Protein kinase superfamily protein

1.0e-54

59.18

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Query:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI
        M+ + +  D SL+LIKQGAE                                      AEARCMTKAR+LGV TPVLYAVD + ++LT EY+EG SVKDI
Subjt:  MEIKTDSNDGSLILIKQGAE--------------------------------------AEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDI

Query:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVLARFGT
         LE G++G   ++L+D+A+QIG AI KLHDGGL HGDLTTSNML+RSGTN+LVLIDFGLS TST+PEDKAVDLYVLERALLSMHSSCGNV+ R  T
Subjt:  LLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVLARFGT

AT5G26110.2 Protein kinase superfamily protein

4.1e-51

75.19

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Query:  MTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDILLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTS
        MTKAR+LGV TPVLYAVD + ++LT EY+EG SVKDI LE G++G   ++L+D+A+QIG AI KLHDGGL HGDLTTSNML+RSGTN+LVLIDFGLS TS
Subjt:  MTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDILLEIGSSG-GSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTS

Query:  TIPEDKAVDLYVLERALLSMHSSCGNVLARFGT
        T+PEDKAVDLYVLERALLSMHSSCGNV+ R  T
Subjt:  TIPEDKAVDLYVLERALLSMHSSCGNVLARFGT

Sequences

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CDS sequence

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ATGGGAAGGATGTGGAGGTCGAGGGATTGTGATGATCTTCATTGTGTGGAAAGGGTGTGGCTCGGGATGGTATTTCTTGACTCATGTAATGTGGAGTATATCGTGAGACA
GACGTGTAATGGTATGGTTGATGATTTGGATCCATATATGAAAAGTATTGCACTCCCATATATGGAGTATTTGGTACAGTATATGGAATGGGATATGGACGAAACCCTTG
TGCTTGAGGCGTTTCTCGCTGCATACTTGAAGGCCGATTCTAGGTGTAATTTGTTCAAACAGAGGCGTCCCAATTTTTCGCTCACATATTCGGTTATGGAGATAAAGACG
GATAGTAATGATGGCTCGCTCATTTTAATTAAGCAAGGAGCTGAAGCTGAAGCAAGATGCATGACAAAAGCTAGGCGGCTAGGAGTTTCTACTCCAGTGTTGTATGCTGT
TGACCCGATACAATATACCCTGACATTTGAGTATGTGGAAGGTCGTTCTGTGAAAGATATATTACTTGAAATTGGGTCAAGTGGTGGTTCAAAACAATTGAATGACATTG
CAAGTCAGATTGGGGTTGCAATTGGAAAGTTGCATGATGGTGGTTTAATTCATGGTGACTTGACCACATCTAACATGTTGATCAGGAGCGGTACCAATGAACTTGTCCTT
ATTGATTTTGGCCTGAGTTTTACTTCAACTATTCCTGAAGATAAAGCAGTTGATCTATATGTGTTGGAACGAGCACTGCTATCAATGCATTCTTCGTGTGGCAACGTGCT
TGCTCGATTTGGAACTGTAAAAGCTTGGTTTCTTGAAGAATCCATACCAGATGGAGCAAATTCTTGCATCATACCGAAAGACTTCGAAGCAATGGTCGTCGACCTCAAAC
AAATTAGCTCAAGTGCGTCAGAGAGGTCGAAAACGCACCATGGTTGGTTAAGAGCTTTCTTCTTGGTCTGTTTTGTTGAATTACCAGTTGACCCCTTGTAG

mRNA sequence

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Protein sequence

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MGRMWRSRDCDDLHCVERVWLGMVFLDSCNVEYIVRQTCNGMVDDLDPYMKSIALPYMEYLVQYMEWDMDETLVLEAFLAAYLKADSRCNLFKQRRPNFSLTYSVMEIKT
DSNDGSLILIKQGAEAEARCMTKARRLGVSTPVLYAVDPIQYTLTFEYVEGRSVKDILLEIGSSGGSKQLNDIASQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVL
IDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVLARFGTVKAWFLEESIPDGANSCIIPKDFEAMVVDLKQISSSASERSKTHHGWLRAFFLVCFVELPVDPL

Spg032258 (gene) of Sponge gourd (cylindrica) v1 genome