CuGenDBv2

Gene ID	Spg033051
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	splicing factor 3B subunit 3
Genome location	scaffold5:6256003..6265323
RNA-Seq Expression	Spg033051
Synteny	Spg033051
Gene Ontology terms	GO:0000398 - mRNA splicing, via spliceosome (biological process) GO:0005686 - U2 snRNP (cellular component) GO:0004523 - RNA-DNA hybrid ribonuclease activity (molecular function) GO:0030620 - U2 snRNA binding (molecular function)
InterPro domains	IPR002156 - Ribonuclease H domain IPR004871 - Cleavage/polyadenylation specificity factor, A subunit, C-terminal IPR012337 - Ribonuclease H-like superfamily IPR036397 - Ribonuclease H superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAG7017534.1 Spliceosome-associated protein, partial [Cucurbita argyrosperma subsp. argyrosperma]

6.3e-72

97.18

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Query:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
        DNASPECNLTLNCAYYMGEIAM+LRKGSFSYKLPADDLLRGC VPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
Subjt:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH

Query:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGS
         EYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSS+VGS
Subjt:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGS

XP_022934513.1 pre-mRNA-splicing factor RSE1 [Cucurbita moschata]

6.3e-72

97.18

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Query:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
        DNASPECNLTLNCAYYMGEIAM+LRKGSFSYKLPADDLLRGC VPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
Subjt:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH

Query:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGS
         EYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSS+VGS
Subjt:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGS

XP_022982808.1 splicing factor 3B subunit 3 [Cucurbita maxima]

6.3e-72

97.18

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Query:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
        DNASPECNLTLNCAYYMGEIAM+LRKGSFSYKLPADDLLRGC VPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
Subjt:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH

Query:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGS
         EYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSS+VGS
Subjt:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGS

XP_023526646.1 splicing factor 3B subunit 3 [Cucurbita pepo subsp. pepo]

6.3e-72

97.18

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Query:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
        DNASPECNLTLNCAYYMGEIAM+LRKGSFSYKLPADDLLRGC VPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
Subjt:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH

Query:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGS
         EYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSS+VGS
Subjt:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGS

XP_038904803.1 splicing factor 3B subunit 3 isoform X1 [Benincasa hispida]

5.7e-73

96.53

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Query:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
        DNASPECNLTLNCAYYMGEIAM+LRKG FSYKLPADDLLRGCAVPGSDFDSSHNT+IASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
Subjt:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH

Query:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGSQS
        YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSS+VGS S
Subjt:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGSQS

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LEB2 Uncharacterized protein

4.0e-72

96.53

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Query:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
        DNASPECNLTLNCAYYMGEIAM+LRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLT PILGNDH
Subjt:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH

Query:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGSQS
        YEYRSRENPIGVPKILDGDILTQFLELTSMQQE VLSS+VGS S
Subjt:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGSQS

A0A1S3B741 pre-mRNA-splicing factor RSE1

9.8e-71

95.14

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Query:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
        DNASPECNLTLNCAYYMGEIAM+LRKGSFSYKLPADDLLRGCA PGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLT PILGNDH
Subjt:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH

Query:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGSQS
        YEYRSRENPIGVPKILDGDILTQFLELTSMQQE VLSS+VG  S
Subjt:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGSQS

A0A5A7TJX6 Pre-mRNA-splicing factor RSE1

9.8e-71

95.14

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Query:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
        DNASPECNLTLNCAYYMGEIAM+LRKGSFSYKLPADDLLRGCA PGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLT PILGNDH
Subjt:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH

Query:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGSQS
        YEYRSRENPIGVPKILDGDILTQFLELTSMQQE VLSS+VG  S
Subjt:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGSQS

A0A6J1F7V8 pre-mRNA-splicing factor RSE1

3.0e-72

97.18

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Query:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
        DNASPECNLTLNCAYYMGEIAM+LRKGSFSYKLPADDLLRGC VPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
Subjt:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH

Query:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGS
         EYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSS+VGS
Subjt:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGS

A0A6J1J5X2 splicing factor 3B subunit 3

3.0e-72

97.18

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Query:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
        DNASPECNLTLNCAYYMGEIAM+LRKGSFSYKLPADDLLRGC VPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH
Subjt:  DNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDH

Query:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGS
         EYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSS+VGS
Subjt:  YEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGS

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT3G11960.1 Cleavage and polyadenylation specificity factor (CPSF) A subunit protein

5.7e-47

65.49

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Query:  ASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDHYE
        +SPE NL LNCAYYMGEIAMS++KG   YKLPADD+LR   +  S  D++ +TIIA TLLGSI +F P+S +EYELLE VQAKL +HPLT P+LGNDH E
Subjt:  ASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILGNDHYE

Query:  YRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGSQS
        +R RENP    KILDGD+L QFLELT+ QQESVLS+   S S
Subjt:  YRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGSQS

AT3G11960.2 Cleavage and polyadenylation specificity factor (CPSF) A subunit protein

3.3e-47

63.95

Show/hide

Query:  DCGDNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILG
        D  + +SPE NL LNCAYYMGEIAMS++KG   YKLPADD+LR   +  S  D++ +TIIA TLLGSI +F P+S +EYELLE VQAKL +HPLT P+LG
Subjt:  DCGDNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELLEAVQAKLAVHPLTCPILG

Query:  NDHYEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGSQS
        NDH E+R RENP    KILDGD+L QFLELT+ QQESVLS+   S S
Subjt:  NDHYEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGSQS

Sequences

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CDS sequence

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ATGATCGAGGAGTTTCTTCCCCATCCTCCTTTTCCAAAACAGGGAAATTTTGTGTGGCAAGCTGAGATTTGTGCTATTGTTTGGGATTGTGGAGATAATGCAAGTCCTGA
ATGCAACTTAACGCTGAACTGTGCTTATTACATGGGTGAAATTGCCATGAGCTTGAGGAAAGGATCTTTTTCATACAAACTTCCAGCTGATGATTTGTTGAGAGGTTGTG
CTGTCCCTGGCTCTGATTTTGACTCATCACACAACACTATTATTGCCAGTACATTATTAGGGAGCATTGTAATCTTCACTCCTCTATCGAGGGATGAGTACGAACTTTTA
GAAGCTGTCCAGGCTAAACTTGCAGTTCATCCACTAACTTGCCCAATTCTGGGGAATGATCATTATGAGTATCGTAGCCGTGAAAACCCAATTGGAGTACCCAAAATACT
TGATGGTGACATACTGACTCAGTTCTTGGAACTTACAAGCATGCAACAAGAGTCAGTATTATCATCAACTGTTGGCTCACAAAGTCTCAATACCTTTGGTGCCTTCCCAT
ACTACACCATCGCTTTCGAGGGGGTGACAACACATACAGCTGCAATGTTTTACAGATTGGTCAGTGTAACTGTTGATATCTTAAAACGATGGCAACTCCTCAAAGTTGCA
TTTCTTACTCGCCTTAGAACTCAAGGACATGGTCGTCCAACCTATAGAACAGATCCTGTTCAAGTAAGGGCGTGCATTTCTTACTCTCTAAAGAGCAGAGCGGTGTCTGT
ACAGGTAGAAAGTTTGCGTGATAGCATTAATTGGAGAGAAGTGAGGAATATTCATAATACTCCCATGACACAACTGATGCCTGAAATGGAGATGCCACATACAGAGATAA
CAAGAGTATGCATCTTTACGGATGCAACAATACATCCTCAAAATGGGAATTCAGGTTATGGAGTGGTGATAGAAGACACTCAAAAACAAATCACAGCTGCAATGATGATG
ATAGATGTCGCTCCTTTGAGTGTCTTGGCAGCGGAGGTGCAAGCAATTTTACAGGGTATTAAAATCATGATCCGAATGGATATGTCTATTGCGAGTCTTTTTACTGATTC
TTTAATTGCTGACAACGTGATTCGTGGAGATGAAGCTCCATCAATGGTAGCGCATCATTGGATTGTCCAAATTCAAGAGTTGTGTAATGGTTAG

mRNA sequence

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Protein sequence

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MIEEFLPHPPFPKQGNFVWQAEICAIVWDCGDNASPECNLTLNCAYYMGEIAMSLRKGSFSYKLPADDLLRGCAVPGSDFDSSHNTIIASTLLGSIVIFTPLSRDEYELL
EAVQAKLAVHPLTCPILGNDHYEYRSRENPIGVPKILDGDILTQFLELTSMQQESVLSSTVGSQSLNTFGAFPYYTIAFEGVTTHTAAMFYRLVSVTVDILKRWQLLKVA
FLTRLRTQGHGRPTYRTDPVQVRACISYSLKSRAVSVQVESLRDSINWREVRNIHNTPMTQLMPEMEMPHTEITRVCIFTDATIHPQNGNSGYGVVIEDTQKQITAAMMM
IDVAPLSVLAAEVQAILQGIKIMIRMDMSIASLFTDSLIADNVIRGDEAPSMVAHHWIVQIQELCNG

Spg033051 (gene) of Sponge gourd (cylindrica) v1 genome